LOCUS       sequence-405168-       15247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid allows for inducible expression of engineered BRD4-p300
            fusion tagged with mNeonGreen, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15247)
  AUTHORS   Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
  TITLE     Molecular features driving condensate formation and gene expression 
            by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
  JOURNAL   Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
  PUBMED    37488172
REFERENCE   2  (bases 1 to 15247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-07-24"; volume: "13(1):11907. doi"; pages: " 
            10.1038/s41598-023-39102-9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-mNeonGreen-BRD4-p300
FEATURES             Location/Qualifiers
     source          1..15247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             990..1016
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1020..1724
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     CDS             7041..8891
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      8655..8664
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(11199..11216)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11205..11429
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11562..11581)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    11713..11760
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             11768..12790
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             11768..12790
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    12920..13041
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12957..12976)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13011..13030
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13090..13106)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13090..13106)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13103..13125)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13114..13130
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13138..13144,13145..13162,13163..13168))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13183..13204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13321..13338)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13492..14080)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13572..13591)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14251..15042,15043..15111))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14874..14893
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15112..15216)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttatgggac cggctgccaa gcgcgtgaaa ctcgatggcg
     1021 tgagcaaggg cgaggaggat aacatggcct ctctcccagc gacacatgag ttacacatct
     1081 ttggctccat caacggtgtg gactttgaca tggtgggtca gggcaccggc aatccaaatg
     1141 atggttatga ggagttaaac ctgaagtcca ccaagggtga cctccagttc tccccctgga
     1201 ttctggtccc tcatatcggg tatggcttcc atcagtacct gccctaccct gacgggatgt
     1261 cgcctttcca ggccgcaatg gtagatggct ccggatacca agtccatcgc acaatgcagt
     1321 ttgaagatgg tgcctccctt actgttaact accgctacac ctacgaggga agccacatca
     1381 aaggagaggc ccaggtgaag gggactggtt tccctgctga cggtcctgtg atgaccaact
     1441 cgctgaccgc tgcggactgg tgcaggtcga agaagactta ccccaacgac aaaaccatca
     1501 tcagtacctt taagtggagt tacaccactg gaaatggcaa gcgctaccgg agcactgcgc
     1561 ggaccaccta cacctttgcc aagccaatgg cggctaacta tctgaagaac cagccgatgt
     1621 acgtgttccg taagacggaa ctcaagcact ccaagaccga actcaacttc aaggagtggc
     1681 aaaaggcctt taccgatgtg atgggcatgg acgagctgta caagggcggc tcgtacgtgt
     1741 acaagtctgc ggagagcggc cctgggacga gattgagaaa tctgccagta atgggggatg
     1801 gactagaaac ttcccaaatg tctacaacac aggcccaggc ccaaccccag ccagccaacg
     1861 cagccagcac caaccccccg cccccagaga cctccaaccc taacaagccc aagaggcaga
     1921 ccaaccaact gcaatacctg ctcagagtgg tgctcaagac actatggaaa caccagtttg
     1981 catggccttt ccagcagcct gtggatgccg tcaagctgaa cctccctgat tactataaga
     2041 tcattaaaac gcctatggat atgggaacaa taaagaagcg cttggaaaac aactattact
     2101 ggaatgctca ggaatgtatc caggacttca acactatgtt tacaaattgt tacatctaca
     2161 acaagcctgg agatgacata gtcttaatgg cagaagctct ggaaaagctc ttcttgcaaa
     2221 aaataaatga gctacccaca gaagaaaccg agatcatgat agtccaggca aaaggaagag
     2281 gacgtgggag gaaagaaaca gggacagcaa aacctggcgt ttccacggta ccaaacacaa
     2341 ctcaagcatc gactcctccg cagacccaga cccctcagcc gaatcctcct cctgtgcagg
     2401 ccacgcctca ccccttccct gccgtcaccc cggacctcat cgtccagacc cctgtcatga
     2461 cagtggtgcc tccccagcca ctgcagacgc ccccgccagt gcccccccag ccacaacccc
     2521 cacccgctcc agctccccag cccgtacaga gccacccacc catcatcgcg gccaccccac
     2581 agcctgtgaa gacaaagaag ggagtgaaga ggaaagcaga caccaccacc cccaccacca
     2641 ttgaccccat tcacgagcca ccctcgctgc ccccggagcc caagaccacc aagctgggcc
     2701 agcggcggga gagcagccgg cctgtgaaac ctccaaagaa ggacgtgccc gactctcagc
     2761 agcacccagc accagagaag agcagcaagg tctcggagca gctcaagtgc tgcagcggca
     2821 tcctcaagga gatgtttgcc aagaagcacg ccgcctacgc ctggcccttc tacaagcctg
     2881 tggacgtgga ggcactgggc ctacacgact actgtgacat catcaagcac cccatggaca
     2941 tgagcacaat caagtctaaa ctggaggccc gtgagtaccg tgatgctcag gagtttggtg
     3001 ctgacgtccg attgatgttc tccaactgct ataagtacaa ccctcctgac catgaggtgg
     3061 tggccatggc ccgcaagctc caggatgtgt tcgaaatgcg ctttgccaag atgccggacg
     3121 agcctgagga gccagtggtg gccgtgtcct ccccggcagt gccccctccc accaaggttg
     3181 tggccccgcc ctcatccagc gacagcagca gcgatagctc ctcggacagt gacagttcga
     3241 ctgatgactc tgaggaggag cgagcccagc ggctggctga gctccaggag cagctcaaag
     3301 ccgtgcacga gcagcttgca gccctctctc agccccagca gaacaaacca aagaaaaagg
     3361 agaaagacaa gaaggaaaag aaaaaagaaa agcacaaaag gaaagaggaa gtggaagaga
     3421 ataaaaaaag caaagccaag gaacctcctc ctaaaaagac gaagaaaaat aatagcagca
     3481 acagcaatgt gagcaagaag gagccagcgc ccatgaagag caagccccct cccacgtatg
     3541 agtcggagga agaggacaag tgcaagccta tgtcctatga ggagaagcgg cagctcagct
     3601 tggacatcaa caagctcccc ggcgagaagc tgggccgcgt ggtgcacatc atccagtcac
     3661 gggagccctc cctgaagaat tccaaccccg acgagattga aatcgacttt gagaccctga
     3721 agccgtccac actgcgtgag ctggagcgct atgtcacctc ctgtttgcgg aagaaaagga
     3781 aacctcaagc tgagaaagtt gatgtgattg ccggctcctc caagatgaag ggcttctcgt
     3841 cctcagagtc ggagagctcc agtgagtcca gctcctctga cagcgaagac tccgaaacaa
     3901 tggccgagaa tgtggtggaa ccggggccgc cttcagccaa gcggcctaaa ctctcatctc
     3961 cggccctctc ggcgtccgcc agcgatggca cagattttgg ctctctattt gacttggagc
     4021 acgacttacc agatgaatta atcaactcta cagaattggg actaaccaat ggtggtgata
     4081 ttaatcagct tcagacaagt cttggcatgg tacaagatgc agcttctaaa cataaacagc
     4141 tgtcagaatt gctgcgatct ggtagttccc ctaacctcaa tatgggagtt ggtggcccag
     4201 gtcaagtcat ggccagccag gcccaacaga gcagtcctgg attaggtttg ataaatagca
     4261 tggtcaaaag cccaatgaca caggcaggct tgacttctcc caacatgggg atgggcacta
     4321 gtggaccaaa tcagggtcct acgcagtcaa caggtatgat gaacagtcca gtaaatcagc
     4381 ctgccatggg aatgaacaca gggatgaatg cgggcatgaa tcctggaatg ttggctgcag
     4441 gcaatggaca agggataatg cctaatcaag tcatgaacgg ttcaattgga gcaggccgag
     4501 ggcgacagaa tatgcagtac ccaaacccag gcatgggaag tgctggcaac ttactgactg
     4561 agcctcttca gcagggctct ccccagatgg gaggacaaac aggattgaga ggcccccagc
     4621 ctcttaagat gggaatgatg aacaacccca atccttatgg ttcaccatat actcagaatc
     4681 ctggacagca gattggagcc agtggccttg gtctccagat tcagacaaaa actgtactat
     4741 caaataactt atctccattt gctatggaca aaaaggcagt tcctggtgga ggaatgccca
     4801 acatgggtca acagccagcc ccgcaggtcc agcagccagg tctggtgact ccagttgccc
     4861 aagggatggg ttctggagca catacagctg atccagagaa gcgcaagctc atccagcagc
     4921 agcttgttct ccttttgcat gctcacaagt gccagcgccg ggaacaggcc aatggggaag
     4981 tgaggcagtg caaccttccc cactgtcgca caatgaagaa tgtcctaaac cacatgacac
     5041 actgccagtc aggcaagtct tgccaagtgg cacactgtgc atcttctcga caaatcattt
     5101 cacactggaa gaattgtaca agacatgatt gtcctgtgtg tctccccctc aaaaatgctg
     5161 gtgataagag aaatcaacag ccaattttga ctggagcacc cgttggactt ggaaatccta
     5221 gctctctagg ggtgggtcaa cagtctgccc ccaacctaag cactgttagt cagattgatc
     5281 ccagctccat agaaagagcc tatgcagctc ttggactacc ctatcaagta aatcagatgc
     5341 cgacacaacc ccaggtgcaa gcaaagaacc agcagaatca gcagcctggg cagtctcccc
     5401 aaggcatgcg gcccatgagc aacatgagtg ctagtcctat gggagtaaat ggaggtgtag
     5461 gagttcaaac gccgagtctt ctttctgact caatgttgca ttcagccata aattctcaaa
     5521 acccaatgat gagtgaaaat gccagtgtgc cctccatggg tcctatgcca acagcagctc
     5581 aaccatccac tactggaatt cggaaacagt ggcacgaaga tattactcag gatcttcgaa
     5641 atcatcttgt tcacaaactc gtccaagcca tatttcctac gccggatcct gctgctttaa
     5701 aagacagacg gatggaaaac ctagttgcat atgctcggaa agttgaaggg gacatgtatg
     5761 aatctgcaaa caatcgagcg gaatactacc accttctagc tgagaaaatc tataagatcc
     5821 agaaagaact agaagaaaaa cgaaggacca gactacagaa gcagaacatg ctaccaaatg
     5881 ctgcaggcat ggttccagtt tccatgaatc cagggcctaa catgggacag ccgcaaccag
     5941 gaatgacttc taatggccct ctacctgacc caagtatgat ccgtggcagt gtgccaaacc
     6001 agatgatgcc tcgaataact ccacaatctg gtttgaatca atttggccag atgagcatgg
     6061 cccagccccc tattgtaccc cggcaaaccc ctcctcttca gcaccatgga cagttggctc
     6121 aacctggagc tctcaacccg cctatgggct atgggcctcg tatgcaacag ccttccaacc
     6181 agggccagtt ccttcctcag actcagttcc catcacaggg aatgaatgta acaaatatcc
     6241 ctttggctcc gtccagcggt caagctccag tgtctcaagc acaaatgtct agttcttcct
     6301 gcccggtgaa ctctcctata atgcctccag ggtctcaggg gagccacatt cactgtcccc
     6361 agcttcctca accagctctt catcagaatt caccctcgcc tgtacctagt cgtaccccca
     6421 cccctcacca tactccccca agcatagggg ctcagcagcc accagcaaca acaattccag
     6481 cccctgttcc tacacctcct gccatgccac ctgggccaca gtcccaggct ctacatcccc
     6541 ctccaaggca gacacctaca ccaccaacaa cacaacttcc ccaacaagtg cagccttcac
     6601 ttcctgctgc accttctgct gaccagcccc agcagcagcc tcgctcacag cagagcacag
     6661 cagcgtctgt tcctacccca acagcaccgc tgcttcctcc gcagcctgca actccacttt
     6721 cccagccagc tgtaagcatt gaaggacagg tatcaaatcc tccatctact agtagcacag
     6781 aagtgaattc tcaggccatt gctgagaagc agccttccca ggaagtgaag atggaggcca
     6841 aaatggaagt ggatcaacca gaaccagcag atactcagcc ggaggatatt tcagagtcta
     6901 aagtggaaga ctgtaaaatg gaatctaccg aaacagaaga gagaagcact gagttaaaaa
     6961 ctgaaataaa agaggaggaa gaccagccaa gtacttcagc tacccagtca tctccggctc
     7021 caggacagtc aaagaaaaag attttcaaac cagaagaact acgacaggca ctgatgccaa
     7081 ctttggaggc actttaccgt caggatccag aatcccttcc ctttcgtcaa cctgtggacc
     7141 ctcagctttt aggaatccct gattactttg atattgtgaa gagccccatg gatctttcta
     7201 ccattaagag gaagttagac actggacagt atcaggagcc ctggcagtat gtcgatgata
     7261 tttggcttat gttcaataat gcctggttat ataaccggaa aacatcacgg gtatacaaat
     7321 actgctccaa gctctctgag gtctttgaac aagaaattga cccagtgatg caaagccttg
     7381 gatactgttg tggcagaaag ttggagttct ctccacagac actgtgttgc tacggcaaac
     7441 agttgtgcac aatacctcgt gatgccactt attacagtta ccagaacagg tatcatttct
     7501 gtgagaagtg tttcaatgag atccaagggg agagcgtttc tttgggggat gacccttccc
     7561 agcctcaaac tacaataaat aaagaacaat tttccaagag aaaaaatgac acactggatc
     7621 ctgaactgtt tgttgaatgt acagagtgcg gaagaaagat gcatcagatc tgtgtccttc
     7681 accatgagat catctggcct gctggattcg tctgtgatgg ctgtttaaag aaaagtgcac
     7741 gaactaggaa agaaaataag ttttctgcta aaaggttgcc atctaccaga cttggcacct
     7801 ttctagagaa tcgtgtgaat gactttctga ggcgacagaa tcaccctgag tcaggagagg
     7861 tcactgttag agtagttcat gcttctgaca aaaccgtgga agtaaaacca ggcatgaaag
     7921 caaggtttgt ggacagtgga gagatggcag aatcctttcc ataccgaacc aaagccctct
     7981 ttgcctttga agaaattgat ggtgttgacc tgtgcttctt tggcatgcat gttcaagagt
     8041 atggctctga ctgccctcca cccaaccaga ggagagtata catatcttac ctcgatagtg
     8101 ttcatttctt ccgtcctaaa tgcttgagga ctgcagtcta tcatgaaatc ctaattggat
     8161 atttagaata tgtcaagaaa ttaggttaca caacagggca tatttgggca tgtccaccaa
     8221 gtgagggaga tgattatatc ttccattgcc atcctcctga ccagaagata cccaagccca
     8281 agcgactgca ggaatggtac aaaaaaatgc ttgacaaggc tgtatcagag cgtattgtcc
     8341 atgactacaa ggatattttt aaacaagcta ctgaagatag attaacaagt gcaaaggaat
     8401 tgccttattt cgagggtgat ttctggccca atgttctgga agaaagcatt aaggaactgg
     8461 aacaggagga agaagagaga aaacgagagg aaaacaccag caatgaaagc acagatgtga
     8521 ccaagggaga cagcaaaaat gctaaaaaga agaataataa gaaaaccagc aaaaataaga
     8581 gcagcctgag taggggcaac aagaagaaac ccgggatgcc caatgtatct aacgacctct
     8641 cacagaaact atatgccacc atggagaagc ataaagaggt cttctttgtg atccgcctca
     8701 ttgctggccc tgctgccaac tccctgcctc ccattgttga tcctgatcct ctcatcccct
     8761 gcgatctgat ggatggtcgg gatgcgtttc tcacgctggc aagggacaag cacctggagt
     8821 tctcttcact ccgaagagcc cagtggtcca ccatgtgcat gctggtggag ctgcacacgc
     8881 agagccagga ccgctttgtc tacacctgca atgaatgcaa gcaccatgtg gagacacgct
     8941 ggcactgtac tgtctgtgag gattatgact tgtgtatcac ctgctataac actaaaaacc
     9001 atgaccacaa aatggagaaa ctaggccttg gcttagatga tgagagcaac aaccagcagg
     9061 ctgcagccac ccagagccca ggcgattctc gccgcctgag tatccagcgc tgcatccagt
     9121 ctctggtcca tgcttgccag tgtcggaatg ccaattgctc actgccatcc tgccagaaga
     9181 tgaagcgggt tgtgcagcat accaagggtt gcaaacggaa aaccaatggc gggtgcccca
     9241 tctgcaagca gctcattgcc ctctgctgct accatgccaa gcactgccag gagaacaaat
     9301 gcccggtgcc gttctgccta aacatcaagc agaagctccg gcagcaacag ctgcagcacc
     9361 gactacagca ggcccaaatg cttcgcagga ggatggccag catgcagcgg actggtgtgg
     9421 ttgggcagca acagggcctc ccttccccca ctcctgccac tccaacgaca ccaactggcc
     9481 aacagccaac caccccgcag acgccccagc ccacttctca gcctcagcct acccctccca
     9541 atagcatgcc accctacttg cccaggactc aagctgctgg ccctgtgtcc cagggtaagg
     9601 cagcaggcca ggtgacccct ccaacccctc ctcagactgc tcagccaccc cttccagggc
     9661 ccccacctgc agcagtggaa atggcaatgc agattcagag agcagcggag acgcagcgcc
     9721 agatggccca cgtgcaaatt tttcaaaggc caatccaaca ccagatgccc ccgatgactc
     9781 ccatggcccc catgggtatg aacccacctc ccatgaccag aggtcccagt gggcatttgg
     9841 agccagggat gggaccgaca gggatgcagc aacagccacc ctggagccaa ggaggattgc
     9901 ctcagcccca gcaactacag tctgggatgc caaggccagc catgatgtca gtggcccagc
     9961 atggtcaacc tttgaacatg gctccacaac caggattggg ccaggtaggt atcagcccac
    10021 tcaaaccagg cactgtgtct caacaagcct tacaaaacct tttgcggact ctcaggtctc
    10081 ccagctctcc cctgcagcag caacaggtgc ttagtatcct tcacgccaac ccccagctgt
    10141 tggctgcatt catcaagcag cgggctgcca agtatgccaa ctctaatcca caacccatcc
    10201 ctgggcagcc tggcatgccc caggggcagc cagggctaca gccacctacc atgccaggtc
    10261 agcagggggt ccactccaat ccagccatgc agaacatgaa tccaatgcag gcgggcgttc
    10321 agagggctgg cctgccccag cagcaaccac agcagcaact ccagccaccc atgggaggga
    10381 tgagccccca ggctcagcag atgaacatga accacaacac catgccttca caattccgag
    10441 acatcttgag acgacagcaa atgatgcaac agcagcagca acagggagca gggccaggaa
    10501 taggccctgg aatggccaac cataaccagt tccagcaacc ccaaggagtt ggctacccac
    10561 cacagcagca gcagcggatg cagcatcaca tgcaacagat gcaacaagga aatatgggac
    10621 agataggcca gcttccccag gccttgggag cagaggcagg tgccagtcta caggcctatc
    10681 agcagcgact ccttcagcaa cagatggggt cccctgttca gcccaacccc atgagccccc
    10741 agcagcatat gctcccaaat caggcccagt ccccacacct acaaggccag cagatcccta
    10801 attctctctc caatcaagtg cgctctcccc agcctgtccc ttctccacgg ccacagtccc
    10861 agccccccca ctccagtcct tccccaagga tgcagcctca gccttctcca caccacgttt
    10921 ccccacagac aagttcccca catcctggac tggtagctgc ccaggccaac cccatggaac
    10981 aagggcattt tgccagcccg gaccagaatt caatgctttc tcagcttgct agcaatccag
    11041 gcatggcaaa cctccatggt gcaagcgcca cggacctggg actcagcacc gataactcag
    11101 acttgaattc aaacctctca cagagtacac tagacatagg ctcgacctga tcatagtgag
    11161 gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
    11221 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
    11281 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
    11341 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
    11401 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
    11461 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
    11521 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
    11581 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
    11641 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
    11701 ttcacgtacc tagaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
    11761 cttggccaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
    11821 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
    11881 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
    11941 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
    12001 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
    12061 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
    12121 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
    12181 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
    12241 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
    12301 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
    12361 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
    12421 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
    12481 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
    12541 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
    12601 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
    12661 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
    12721 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
    12781 aaaggaatag cacgtactac gagatttcga ttccaccgcc gccttctatg aaaggttggg
    12841 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
    12901 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
    12961 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    13021 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
    13081 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    13141 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    13201 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    13261 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    13321 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    13381 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    13441 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    13501 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    13561 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    13621 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    13681 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    13741 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    13801 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    13861 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    13921 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    13981 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    14041 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    14101 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    14161 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    14221 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    14281 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    14341 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    14401 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    14461 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    14521 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    14581 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    14641 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    14701 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    14761 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    14821 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    14881 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    14941 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    15001 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    15061 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    15121 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    15181 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    15241 tgacgtc
//
