LOCUS       sequence-405169-       11457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid allows for inducible expression of full-length BRD4-NUT
            fusion tagged with mNeonGreen, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11457)
  AUTHORS   Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
  TITLE     Molecular features driving condensate formation and gene expression 
            by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
  JOURNAL   Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
  PUBMED    37488172
REFERENCE   2  (bases 1 to 11457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-07-24"; volume: "13(1):11907. doi"; pages: " 
            10.1038/s41598-023-39102-9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT
FEATURES             Location/Qualifiers
     source          1..11457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1058..1084
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1088..1792
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     primer_bind     complement(7409..7426)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7415..7639
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7772..7791)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7923..7970
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7978..9000
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7978..9000
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9130..9251
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9167..9186)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9221..9240
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9300..9316)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9300..9316)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9313..9335)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9324..9340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9348..9354,9355..9372,9373..9378))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9393..9414
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9531..9548)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9702..10290)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9782..9801)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10461..11252,11253..11321))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11084..11103
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11322..11426)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     1021 attctgcaga tatccagcac agtggcggcc gatgggaccg gctgccaagc gcgtgaaact
     1081 cgatggcgtg agcaagggcg aggaggataa catggcctct ctcccagcga cacatgagtt
     1141 acacatcttt ggctccatca acggtgtgga ctttgacatg gtgggtcagg gcaccggcaa
     1201 tccaaatgat ggttatgagg agttaaacct gaagtccacc aagggtgacc tccagttctc
     1261 cccctggatt ctggtccctc atatcgggta tggcttccat cagtacctgc cctaccctga
     1321 cgggatgtcg cctttccagg ccgcaatggt agatggctcc ggataccaag tccatcgcac
     1381 aatgcagttt gaagatggtg cctcccttac tgttaactac cgctacacct acgagggaag
     1441 ccacatcaaa ggagaggccc aggtgaaggg gactggtttc cctgctgacg gtcctgtgat
     1501 gaccaactcg ctgaccgctg cggactggtg caggtcgaag aagacttacc ccaacgacaa
     1561 aaccatcatc agtaccttta agtggagtta caccactgga aatggcaagc gctaccggag
     1621 cactgcgcgg accacctaca cctttgccaa gccaatggcg gctaactatc tgaagaacca
     1681 gccgatgtac gtgttccgta agacggaact caagcactcc aagaccgaac tcaacttcaa
     1741 ggagtggcaa aaggccttta ccgatgtgat gggcatggac gagctgtaca agggcggctc
     1801 gtacgtgtac aagtctgcgg agagcggccc tgggacgaga ttgagaaatc tgccagtaat
     1861 gggggatgga ctagaaactt cccaaatgtc tacaacacag gcccaggccc aaccccagcc
     1921 agccaacgca gccagcacca accccccgcc cccagagacc tccaacccta acaagcccaa
     1981 gaggcagacc aaccaactgc aatacctgct cagagtggtg ctcaagacac tatggaaaca
     2041 ccagtttgca tggcctttcc agcagcctgt ggatgccgtc aagctgaacc tccctgatta
     2101 ctataagatc attaaaacgc ctatggatat gggaacaata aagaagcgct tggaaaacaa
     2161 ctattactgg aatgctcagg aatgtatcca ggacttcaac actatgttta caaattgtta
     2221 catctacaac aagcctggag atgacatagt cttaatggca gaagctctgg aaaagctctt
     2281 cttgcaaaaa ataaatgagc tacccacaga agaaaccgag atcatgatag tccaggcaaa
     2341 aggaagagga cgtgggagga aagaaacagg gacagcaaaa cctggcgttt ccacggtacc
     2401 aaacacaact caagcatcga ctcctccgca gacccagacc cctcagccga atcctcctcc
     2461 tgtgcaggcc acgcctcacc ccttccctgc cgtcaccccg gacctcatcg tccagacccc
     2521 tgtcatgaca gtggtgcctc cccagccact gcagacgccc ccgccagtgc ccccccagcc
     2581 acaaccccca cccgctccag ctccccagcc cgtacagagc cacccaccca tcatcgcggc
     2641 caccccacag cctgtgaaga caaagaaggg agtgaagagg aaagcagaca ccaccacccc
     2701 caccaccatt gaccccattc acgagccacc ctcgctgccc ccggagccca agaccaccaa
     2761 gctgggccag cggcgggaga gcagccggcc tgtgaaacct ccaaagaagg acgtgcccga
     2821 ctctcagcag cacccagcac cagagaagag cagcaaggtc tcggagcagc tcaagtgctg
     2881 cagcggcatc ctcaaggaga tgtttgccaa gaagcacgcc gcctacgcct ggcccttcta
     2941 caagcctgtg gacgtggagg cactgggcct acacgactac tgtgacatca tcaagcaccc
     3001 catggacatg agcacaatca agtctaaact ggaggcccgt gagtaccgtg atgctcagga
     3061 gtttggtgct gacgtccgat tgatgttctc caactgctat aagtacaacc ctcctgacca
     3121 tgaggtggtg gccatggccc gcaagctcca ggatgtgttc gaaatgcgct ttgccaagat
     3181 gccggacgag cctgaggagc cagtggtggc cgtgtcctcc ccggcagtgc cccctcccac
     3241 caaggttgtg gccccgccct catccagcga cagcagcagc gatagctcct cggacagtga
     3301 cagttcgact gatgactctg aggaggagcg agcccagcgg ctggctgagc tccaggagca
     3361 gctcaaagcc gtgcacgagc agcttgcagc cctctctcag ccccagcaga acaaaccaaa
     3421 gaaaaaggag aaagacaaga aggaaaagaa aaaagaaaag cacaaaagga aagaggaagt
     3481 ggaagagaat aaaaaaagca aagccaagga acctcctcct aaaaagacga agaaaaataa
     3541 tagcagcaac agcaatgtga gcaagaagga gccagcgccc atgaagagca agccccctcc
     3601 cacgtatgag tcggaggaag aggacaagtg caagcctatg tcctatgagg agaagcggca
     3661 gctcagcttg gacatcaaca agctccccgg cgagaagctg ggccgcgtgg tgcacatcat
     3721 ccagtcacgg gagccctccc tgaagaattc caaccccgac gagattgaaa tcgactttga
     3781 gaccctgaag ccgtccacac tgcgtgagct ggagcgctat gtcacctcct gtttgcggaa
     3841 gaaaaggaaa cctcaagctg agaaagttga tgtgattgcc ggctcctcca agatgaaggg
     3901 cttctcgtcc tcagagtcgg agagctccag tgagtccagc tcctctgaca gcgaagactc
     3961 cgaaacagca tctgcattgc cgggaccgga tatgagcatg aaacctagtg ccgccctgtc
     4021 tccatcccct gcacttccct ttctcccacc aacttctgac ccaccagacc acccacccag
     4081 ggagccacct ccacagccca tcatgccttc agtattctct ccagacaacc ctctgatgct
     4141 ctctgctttc cccagctcac tgttggtgac aggggacggg ggcccttgcc tcagtggggc
     4201 tggggctggc aaggtcattg tcaaagtcaa gacagaaggg gggtcagctg agccctctca
     4261 aactcagaac tttatcctta ctcagactgc cctcaattcg actgccccgg gcactccctg
     4321 tggaggcctt gagggtcctg cacctccatt tgtgacagca tctaatgtga agaccattct
     4381 gccctctaag gctgttggtg tcagccagga gggtcctcca ggccttccgc ctcagcctcc
     4441 accaccagtt gctcaactgg tccccattgt gcccctggaa aaagcttggc cagggccaca
     4501 tgggacaacc ggggaaggag gtcctgtggc cactctatcc aagccttccc taggtgaccg
     4561 ctccaaaatt tccaaggacg tttatgagaa cttccgtcag tggcagcgtt acaaagcctt
     4621 ggcccggagg cacctatccc agagtcctga cacagaagct ctttcctgtt ttcttatccc
     4681 agtgcttcgt tccctggccc ggctgaagcc cactatgacc ctggaggagg gactgccatt
     4741 ggctgtgcag gagtgggagc acaccagcaa ctttgaccgg atgatctttt atgagatggc
     4801 agaaaggttc atggagtttg aggctgagga gatgcagatt cagaacacac agctgatgaa
     4861 tgggtctcag ggcctgtctc ctgcaacccc tttgaaactt gatcctctag ggcccctggc
     4921 ctctgaggtt tgccagcagc cagtgtacat tccgaagaag gcagcctcca agacacgggc
     4981 cccccgccgg cgtcagcgta aagcccagag acctcctgct cctgaggcac ccaaggagat
     5041 cccaccagaa gctgtgaagg agtatgttga catcatggaa tggctggtgg ggactcactt
     5101 ggccactggg gagtcagatg gaaaacaaga ggaagaaggg cagcagcagg aggaggaagg
     5161 gatgtatcca gatccaggtc tcctgagcta catcaatgag ctgtgttctc agaaggtctt
     5221 tgtctccaag gtggaggctg tcattcaccc tcaatttctg gcagatctgc tgtccccaga
     5281 aaaacagaga gatcccttgg ccttaattga ggagctagag caagaagaag gactcactct
     5341 tgcccagctg gtccagaagc gactcatggc cttggaagag gaggaagatg cagaggcgcc
     5401 tccaagtttc agtggcgctc agttggactc aagtccttct ggttctgttg aggatgaaga
     5461 tggggatggg cggcttcggc cctcacctgg gcttcagggg gctgggggcg ccgcttgcct
     5521 tggaaaggtt tcttcttcag gaaaacgggc aagagaagtg catggtgggc aggagcaagc
     5581 cctagatagc cccagaggga tgcacaggga tgggaacact ctgccatccc ccagcagctg
     5641 ggacctgcag ccagaacttg cagctccaca gggaactccg ggacccttgg gtgtggagag
     5701 gagagggtct gggaaggtta taaaccaggt atctctacat caggatggcc atctaggagg
     5761 cgctgggcct cctgggcact gcctggtggc tgataggact tcagaggctc tgcccctttg
     5821 ttggcaggga ggcttccagc ctgagagcac tcccagtttg gatgctggac ttgcagagct
     5881 ggctcctctg caaggacaag ggttagaaaa gcaagtcctg ggattgcaga aaggacaaca
     5941 aacagggggt cgtggagtgc ttcctcaagg gaaggagcct ttagcagtgc cctgggaagg
     6001 ctcttcagga gccatgtggg gagatgacag aggtaccccc atggctcaga gttatgatca
     6061 gaatccttcc cctagagcag ctggggagag ggacgatgtc tgtctcagcc caggagtttg
     6121 gctgagcagt gagatggatg ctgtaggctt ggagctgcct gtacaaatag aggaggtcat
     6181 agagagcttc caagttgaga agtgtgtaac tgagtatcag gaaggctgcc agggactggg
     6241 ctccaggggc aacatttccc tgggtcctgg agaaacccta gtacctgggg atacggagag
     6301 cagtgtgatt ccctgtggag gcacagttgc ggcagctgcc ctagaaaaga gaaactattg
     6361 cagcttgcca ggacctttga gggccaacag cccacccttg aggtccaaag aaaatcaaga
     6421 acagagctgt gaaaccgtag ggcatcccag tgatctgtgg gcagaaggtt gcttcccatt
     6481 gctagaaagt ggtgattcca cactggggtc ttccaaagaa acccttccac ccacatgcca
     6541 aggcaatctc cttatcatgg ggactgagga tgcctcctcc ttgcctgaag ccagccaaga
     6601 ggcagggagc agaggcaatt ccttttctcc tctgttggaa accatagaac ctgtcaacat
     6661 actagatgtt aaagatgact gtggcctcca actaagggtc agcgaggaca cctgcccact
     6721 gaatgttcat tcttatgacc cccaaggaga aggcagggtg gatcctgatc tgtccaagcc
     6781 taaaaacctt gctcctttac aagagagtca ggagtcttac acaactggga ctcccaaagc
     6841 aacatcttct caccagggcc ttggaagcac tttgcctaga aggggaacca ggaatgccat
     6901 agttccgaga gaaacttctg ttagtaaaac acacaggtca gcagacaggg ccaaaggaaa
     6961 ggagaaaaag aaaaaggaag cagaggaaga ggatgaggaa ctctccaact ttgcttacct
     7021 cttggcctct aaacttagcc tctcaccaag ggagcatccc ctcagtcctc accatgcctc
     7081 aggaggtcag ggcagccaga gagcatccca cctgctccct gctggagcaa aaggccccag
     7141 caaacttcca tatcctgttg ccaagtctgg gaagcgagct ctagctggag gtccagcccc
     7201 tactgaaaag acaccccact caggagctca acttggggtc cccagggaga aacccctagc
     7261 tctgggagta gttcgaccct cacagcctcg taaaaggcgg tgtgacagtt ttgtcacggg
     7321 cagaaggaag aaacgacgtc gtagccaggg ctcgacctga tcatagtgag gctcgagtct
     7381 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     7441 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     7501 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     7561 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     7621 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     7681 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7741 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     7801 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     7861 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtacc
     7921 tagaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc cttggccaaa
     7981 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     8041 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     8101 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
     8161 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
     8221 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
     8281 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
     8341 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
     8401 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     8461 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     8521 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
     8581 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     8641 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
     8701 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
     8761 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     8821 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     8881 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     8941 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
     9001 cacgtactac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     9061 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     9121 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9181 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9241 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     9301 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9361 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9421 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9481 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9661 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9721 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9781 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9841 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9901 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9961 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    10021 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    10081 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10141 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10201 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10261 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10321 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10381 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10441 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10501 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10561 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10621 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10681 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    10741 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    10801 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    10861 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    10921 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    10981 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    11041 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    11101 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    11161 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    11221 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    11281 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11341 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11401 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
