LOCUS       sequence-405170-       12000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid allows for inducible expression of engineered 
            BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12000)
  AUTHORS   Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
  TITLE     Molecular features driving condensate formation and gene expression 
            by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
  JOURNAL   Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
  PUBMED    37488172
REFERENCE   2  (bases 1 to 12000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-07-24"; volume: "13(1):11907. doi"; pages: " 
            10.1038/s41598-023-39102-9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(I)
FEATURES             Location/Qualifiers
     source          1..12000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             990..1016
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1020..1724
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     primer_bind     complement(7952..7969)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7958..8182
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8315..8334)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8466..8513
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8521..9543
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8521..9543
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9673..9794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9710..9729)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9764..9783
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9843..9859)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9843..9859)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9856..9878)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9867..9883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9891..9897,9898..9915,9916..9921))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9936..9957
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10074..10091)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10245..10833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10325..10344)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11004..11795,11796..11864))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11627..11646
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11865..11969)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttatgggac cggctgccaa gcgcgtgaaa ctcgatggcg
     1021 tgagcaaggg cgaggaggat aacatggcct ctctcccagc gacacatgag ttacacatct
     1081 ttggctccat caacggtgtg gactttgaca tggtgggtca gggcaccggc aatccaaatg
     1141 atggttatga ggagttaaac ctgaagtcca ccaagggtga cctccagttc tccccctgga
     1201 ttctggtccc tcatatcggg tatggcttcc atcagtacct gccctaccct gacgggatgt
     1261 cgcctttcca ggccgcaatg gtagatggct ccggatacca agtccatcgc acaatgcagt
     1321 ttgaagatgg tgcctccctt actgttaact accgctacac ctacgaggga agccacatca
     1381 aaggagaggc ccaggtgaag gggactggtt tccctgctga cggtcctgtg atgaccaact
     1441 cgctgaccgc tgcggactgg tgcaggtcga agaagactta ccccaacgac aaaaccatca
     1501 tcagtacctt taagtggagt tacaccactg gaaatggcaa gcgctaccgg agcactgcgc
     1561 ggaccaccta cacctttgcc aagccaatgg cggctaacta tctgaagaac cagccgatgt
     1621 acgtgttccg taagacggaa ctcaagcact ccaagaccga actcaacttc aaggagtggc
     1681 aaaaggcctt taccgatgtg atgggcatgg acgagctgta caagggcggc tcgtacgtgt
     1741 acaagtctgc ggagagcggc cctgggacga gattgagaaa tctgccagta atgggggatg
     1801 gactagaaac ttcccaaatg tctacaacac aggcccaggc ccaaccccag ccagccaacg
     1861 cagccagcac caaccccccg cccccagaga cctccaaccc taacaagccc aagaggcaga
     1921 ccaaccaact gcaatacctg ctcagagtgg tgctcaagac actatggaaa caccagtttg
     1981 catggccttt ccagcagcct gtggatgccg tcaagctgaa cctccctgat tactataaga
     2041 tcattaaaac gcctatggat atgggaacaa taaagaagcg cttggaaaac aactattact
     2101 ggaatgctca ggaatgtatc caggacttca acactatgtt tacaaattgt tacatctaca
     2161 acaagcctgg agatgacata gtcttaatgg cagaagctct ggaaaagctc ttcttgcaaa
     2221 aaataaatga gctacccaca gaagaaaccg agatcatgat agtccaggca aaaggaagag
     2281 gacgtgggag gaaagaaaca gggacagcaa aacctggcgt ttccacggta ccaaacacaa
     2341 ctcaagcatc gactcctccg cagacccaga cccctcagcc gaatcctcct cctgtgcagg
     2401 ccacgcctca ccccttccct gccgtcaccc cggacctcat cgtccagacc cctgtcatga
     2461 cagtggtgcc tccccagcca ctgcagacgc ccccgccagt gcccccccag ccacaacccc
     2521 cacccgctcc agctccccag cccgtacaga gccacccacc catcatcgcg gccaccccac
     2581 agcctgtgaa gacaaagaag ggagtgaaga ggaaagcaga caccaccacc cccaccacca
     2641 ttgaccccat tcacgagcca ccctcgctgc ccccggagcc caagaccacc aagctgggcc
     2701 agcggcggga gagcagccgg cctgtgaaac ctccaaagaa ggacgtgccc gactctcagc
     2761 agcacccagc accagagaag agcagcaagg tctcggagca gctcaagtgc tgcagcggca
     2821 tcctcaagga gatgtttgcc aagaagcacg ccgcctacgc ctggcccttc tacaagcctg
     2881 tggacgtgga ggcactgggc ctacacgact actgtgacat catcaagcac cccatggaca
     2941 tgagcacaat caagtctaaa ctggaggccc gtgagtaccg tgatgctcag gagtttggtg
     3001 ctgacgtccg attgatgttc tccaactgct ataagtacaa ccctcctgac catgaggtgg
     3061 tggccatggc ccgcaagctc caggatgtgt tcgaaatgcg ctttgccaag atgccggacg
     3121 agcctgagga gccagtggtg gccgtgtcct ccccggcagt gccccctccc accaaggttg
     3181 tggccccgcc ctcatccagc gacagcagca gcgatagctc ctcggacagt gacagttcga
     3241 ctgatgactc tgaggaggag cgagcccagc ggctggctga gctccaggag cagctcaaag
     3301 ccgtgcacga gcagcttgca gccctctctc agccccagca gaacaaacca aagaaaaagg
     3361 agaaagacaa gaaggaaaag aaaaaagaaa agcacaaaag gaaagaggaa gtggaagaga
     3421 ataaaaaaag caaagccaag gaacctcctc ctaaaaagac gaagaaaaat aatagcagca
     3481 acagcaatgt gagcaagaag gagccagcgc ccatgaagag caagccccct cccacgtatg
     3541 agtcggagga agaggacaag tgcaagccta tgtcctatga ggagaagcgg cagctcagct
     3601 tggacatcaa caagctcccc ggcgagaagc tgggccgcgt ggtgcacatc atccagtcac
     3661 gggagccctc cctgaagaat tccaaccccg acgagattga aatcgacttt gagaccctga
     3721 agccgtccac actgcgtgag ctggagcgct atgtcacctc ctgtttgcgg aagaaaagga
     3781 aacctcaagc tgagaaagtt gatgtgattg ccggctcctc caagatgaag ggcttctcgt
     3841 cctcagagtc ggagagctcc agtgagtcca gctcctctga cagcgaagac tccgaaacaa
     3901 tggccgagaa tgtggtggaa ccggggccgc cttcagccaa gcggcctaaa ctctcatctc
     3961 cggccctctc ggcgtccgcc agcgatggca cagattttgg ctctctattt gacttggagc
     4021 acgacttacc agatgaatta atcaactcta cagaattggg actaaccaat ggtggtgata
     4081 ttaatcagct tcagacaagt cttggcatgg tacaagatgc agcttctaaa cataaacagc
     4141 tgtcagaatt gctgcgatct ggtagttccc ctaacctcaa tatgggagtt ggtggcccag
     4201 gtcaagtcat ggccagccag gcccaacaga gcagtcctgg attaggtttg ataaatagca
     4261 tggtcaaaag cccaatgaca caggcaggct tgacttctcc caacatgggg atgggcacta
     4321 gtggaccaaa tcagggtcct acgcagtcaa caggtatgat gaacagtcca gtaaatcagc
     4381 ctgccatggg aatgaacaca gggatgaatg cgggcatgaa tcctggaatg ttggctgcag
     4441 gcaatggaca agggataatg cctaatcaag tcatgaacgg ttcaattgga gcaggccgag
     4501 ggcgacagaa tatgcagtac ccaaacccag gcatgggaag tgctggcaac ttactgactg
     4561 agcctcttca gcagggctct ccccagatgg gaggacaaac aggattgaga ggcccccagc
     4621 ctcttaagat gggaatgatg aacaacccca atccttatgg ttcaccatat actcagaatc
     4681 ctggacagca gattggagcc agtggccttg gtctccagat tcagacaaaa actgtactat
     4741 caaataactt atctccattt gctatggaca aaaaggcagt tcctggtgga ggaatgccca
     4801 acatgggtca acagccagcc ccgcaggtcc agcagccagg tctggtgact ccagttgccc
     4861 aagggatggg ttctggagca catacagctg aaaaacgaag gaccagacta cagaagcaga
     4921 acatgctacc aaatgctgca ggcatggttc cagtttccat gaatccaggg cctaacatgg
     4981 gacagccgca accaggaatg acttctaatg gccctctacc tgacccaagt atgatccgtg
     5041 gcagtgtgcc aaaccagatg atgcctcgaa taactccaca atctggtttg aatcaatttg
     5101 gccagatgag catggcccag ccccctattg taccccggca aacccctcct cttcagcacc
     5161 atggacagtt ggctcaacct ggagctctca acccgcctat gggctatggg cctcgtatgc
     5221 aacagccttc caaccagggc cagttccttc ctcagactca gttcccatca cagggaatga
     5281 atgtaacaaa tatccctttg gctccgtcca gcggtcaagc tccagtgtct caagcacaaa
     5341 tgtctagttc ttcctgcccg gtgaactctc ctataatgcc tccagggtct caggggagcc
     5401 acattcactg tccccagctt cctcaaccag ctcttcatca gaattcaccc tcgcctgtac
     5461 ctagtcgtac ccccacccct caccatactc ccccaagcat aggggctcag cagccaccag
     5521 caacaacaat tccagcccct gttcctacac ctcctgccat gccacctggg ccacagtccc
     5581 aggctctaca tccccctcca aggcagacac ctacaccacc aacaacacaa cttccccaac
     5641 aagtgcagcc ttcacttcct gctgcacctt ctgctgacca gccccagcag cagcctcgct
     5701 cacagcagag cacagcagcg tctgttccta ccccaacagc accgctgctt cctccgcagc
     5761 ctgcaactcc actttcccag ccagctgtaa gcattgaagg acaggtatca aatcctccat
     5821 ctactagtag cacagaagtg aattctcagg ccattgctga gaagcagcct tcccaggaag
     5881 tgaagatgga ggccaaaatg gaagtggatc aaccagaacc agcagatact cagccggagg
     5941 atatttcaga gtctaaagtg gaagactgta aaatggaatc taccgaaaca gaagagagaa
     6001 gcactgagtt aaaaactgaa ataaaagagg aggaagacca gccaagtact tcagctaccc
     6061 agtcatctcc ggctccagga cagtcaaaga aaaagcagaa gctccggcag caacagctgc
     6121 agcaccgact acagcaggcc caaatgcttc gcaggaggat ggccagcatg cagcggactg
     6181 gtgtggttgg gcagcaacag ggcctccctt cccccactcc tgccactcca acgacaccaa
     6241 ctggccaaca gccaaccacc ccgcagacgc cccagcccac ttctcagcct cagcctaccc
     6301 ctcccaatag catgccaccc tacttgccca ggactcaagc tgctggccct gtgtcccagg
     6361 gtaaggcagc aggccaggtg acccctccaa cccctcctca gactgctcag ccaccccttc
     6421 cagggccccc acctgcagca gtggaaatgg caatgcagat tcagagagca gcggagacgc
     6481 agcgccagat ggcccacgtg caaatttttc aaaggccaat ccaacaccag atgcccccga
     6541 tgactcccat ggcccccatg ggtatgaacc cacctcccat gaccagaggt cccagtgggc
     6601 atttggagcc agggatggga ccgacaggga tgcagcaaca gccaccctgg agccaaggag
     6661 gattgcctca gccccagcaa ctacagtctg ggatgccaag gccagccatg atgtcagtgg
     6721 cccagcatgg tcaacctttg aacatggctc cacaaccagg attgggccag gtaggtatca
     6781 gcccactcaa accaggcact gtgtctcaac aagccttaca aaaccttttg cggactctca
     6841 ggtctcccag ctctcccctg cagcagcaac aggtgcttag tatccttcac gccaaccccc
     6901 agctgttggc tgcattcatc aagcagcggg ctgccaagta tgccaactct aatccacaac
     6961 ccatccctgg gcagcctggc atgccccagg ggcagccagg gctacagcca cctaccatgc
     7021 caggtcagca gggggtccac tccaatccag ccatgcagaa catgaatcca atgcaggcgg
     7081 gcgttcagag ggctggcctg ccccagcagc aaccacagca gcaactccag ccacccatgg
     7141 gagggatgag cccccaggct cagcagatga acatgaacca caacaccatg ccttcacaat
     7201 tccgagacat cttgagacga cagcaaatga tgcaacagca gcagcaacag ggagcagggc
     7261 caggaatagg ccctggaatg gccaaccata accagttcca gcaaccccaa ggagttggct
     7321 acccaccaca gcagcagcag cggatgcagc atcacatgca acagatgcaa caaggaaata
     7381 tgggacagat aggccagctt ccccaggcct tgggagcaga ggcaggtgcc agtctacagg
     7441 cctatcagca gcgactcctt cagcaacaga tggggtcccc tgttcagccc aaccccatga
     7501 gcccccagca gcatatgctc ccaaatcagg cccagtcccc acacctacaa ggccagcaga
     7561 tccctaattc tctctccaat caagtgcgct ctccccagcc tgtcccttct ccacggccac
     7621 agtcccagcc cccccactcc agtccttccc caaggatgca gcctcagcct tctccacacc
     7681 acgtttcccc acagacaagt tccccacatc ctggactggt agctgcccag gccaacccca
     7741 tggaacaagg gcattttgcc agcccggacc agaattcaat gctttctcag cttgctagca
     7801 atccaggcat ggcaaacctc catggtgcaa gcgccacgga cctgggactc agcaccgata
     7861 actcagactt gaattcgaac ctctcacaga gtacactaga catatgagcg gccgctcgag
     7921 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     7981 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     8041 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     8101 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     8161 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     8221 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8281 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     8341 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8401 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     8461 acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttccttggcc
     8521 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     8581 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     8641 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     8701 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     8761 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     8821 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     8881 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     8941 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     9001 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     9061 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     9121 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     9181 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     9241 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     9301 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     9361 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     9421 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     9481 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     9541 tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     9601 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     9661 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     9721 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9781 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     9841 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     9901 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     9961 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    10021 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    10081 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    10141 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    10201 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    10261 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    10321 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    10381 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    10441 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    10501 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    10561 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    10621 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    10681 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    10741 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    10801 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    10861 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    10921 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    10981 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    11041 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    11101 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    11161 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    11221 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    11281 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    11341 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    11401 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    11461 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    11521 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    11581 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    11641 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    11701 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    11761 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    11821 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    11881 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    11941 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
