LOCUS       sequence-405171-        8529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid allows for inducible expression of BRD4-NUT(MIN) mutant
            fusion tagged with mNeonGreen, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
  TITLE     Molecular features driving condensate formation and gene expression 
            by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
  JOURNAL   Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
  PUBMED    37488172
REFERENCE   2  (bases 1 to 8529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-07-24"; volume: "13(1):11907. doi"; pages: " 
            10.1038/s41598-023-39102-9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(MIN)
FEATURES             Location/Qualifiers
     source          1..8529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1058..1084
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1088..1792
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     primer_bind     complement(4481..4498)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4487..4711
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4844..4863)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4995..5042
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5050..6072
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5050..6072
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6202..6323
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6239..6258)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6293..6312
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6372..6388)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6372..6388)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6385..6407)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6396..6412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6420..6426,6427..6444,6445..6450))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6465..6486
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6603..6620)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6774..7362)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6854..6873)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7533..8324,8325..8393))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8156..8175
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8394..8498)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     1021 attctgcaga tatccagcac agtggcggcc gatgggaccg gctgccaagc gcgtgaaact
     1081 cgatggcgtg agcaagggcg aggaggataa catggcctct ctcccagcga cacatgagtt
     1141 acacatcttt ggctccatca acggtgtgga ctttgacatg gtgggtcagg gcaccggcaa
     1201 tccaaatgat ggttatgagg agttaaacct gaagtccacc aagggtgacc tccagttctc
     1261 cccctggatt ctggtccctc atatcgggta tggcttccat cagtacctgc cctaccctga
     1321 cgggatgtcg cctttccagg ccgcaatggt agatggctcc ggataccaag tccatcgcac
     1381 aatgcagttt gaagatggtg cctcccttac tgttaactac cgctacacct acgagggaag
     1441 ccacatcaaa ggagaggccc aggtgaaggg gactggtttc cctgctgacg gtcctgtgat
     1501 gaccaactcg ctgaccgctg cggactggtg caggtcgaag aagacttacc ccaacgacaa
     1561 aaccatcatc agtaccttta agtggagtta caccactgga aatggcaagc gctaccggag
     1621 cactgcgcgg accacctaca cctttgccaa gccaatggcg gctaactatc tgaagaacca
     1681 gccgatgtac gtgttccgta agacggaact caagcactcc aagaccgaac tcaacttcaa
     1741 ggagtggcaa aaggccttta ccgatgtgat gggcatggac gagctgtaca agggcggctc
     1801 gtacgtgtac aagtctgcgg agagcggccc tgggacgaga ttgagaaatc tgccagtaat
     1861 gggggatgga ctagaaactt cccaaatgtc tacaacacag gcccaggccc aaccccagcc
     1921 agccaacgca gccagcacca accccccgcc cccagagacc tccaacccta acaagcccaa
     1981 gaggcagacc aaccaactgc aatacctgct cagagtggtg ctcaagacac tatggaaaca
     2041 ccagtttgca tggcctttcc agcagcctgt ggatgccgtc aagctgaacc tccctgatta
     2101 ctataagatc attaaaacgc ctatggatat gggaacaata aagaagcgct tggaaaacaa
     2161 ctattactgg aatgctcagg aatgtatcca ggacttcaac actatgttta caaattgtta
     2221 catctacaac aagcctggag atgacatagt cttaatggca gaagctctgg aaaagctctt
     2281 cttgcaaaaa ataaatgagc tacccacaga agaaaccgag atcatgatag tccaggcaaa
     2341 aggaagagga cgtgggagga aagaaacagg gacagcaaaa cctggcgttt ccacggtacc
     2401 aaacacaact caagcatcga ctcctccgca gacccagacc cctcagccga atcctcctcc
     2461 tgtgcaggcc acgcctcacc ccttccctgc cgtcaccccg gacctcatcg tccagacccc
     2521 tgtcatgaca gtggtgcctc cccagccact gcagacgccc ccgccagtgc ccccccagcc
     2581 acaaccccca cccgctccag ctccccagcc cgtacagagc cacccaccca tcatcgcggc
     2641 caccccacag cctgtgaaga caaagaaggg agtgaagagg aaagcagaca ccaccacccc
     2701 caccaccatt gaccccattc acgagccacc ctcgctgccc ccggagccca agaccaccaa
     2761 gctgggccag cggcgggaga gcagccggcc tgtgaaacct ccaaagaagg acgtgcccga
     2821 ctctcagcag cacccagcac cagagaagag cagcaaggtc tcggagcagc tcaagtgctg
     2881 cagcggcatc ctcaaggaga tgtttgccaa gaagcacgcc gcctacgcct ggcccttcta
     2941 caagcctgtg gacgtggagg cactgggcct acacgactac tgtgacatca tcaagcaccc
     3001 catggacatg agcacaatca agtctaaact ggaggcccgt gagtaccgtg atgctcagga
     3061 gtttggtgct gacgtccgat tgatgttctc caactgctat aagtacaacc ctcctgacca
     3121 tgaggtggtg gccatggccc gcaagctcca ggatgtgttc gaaatgcgct ttgccaagat
     3181 gccggacgag cctgaggagc cagtggtggc cgtgtcctcc ccggcagtgc cccctcccac
     3241 caaggttgtg gccccgccct catccagcga cagcagcagc gatagctcct cggacagtga
     3301 cagttcgact gatgactctg aggaggagcg agcccagcgg ctggctgagc tccaggagca
     3361 gctcaaagcc gtgcacgagc agcttgcagc cctctctcag ccccagcaga acaaaccaaa
     3421 gaaaaaggag aaagacaaga aggaaaagaa aaaagaaaag cacaaaagga aagaggaagt
     3481 ggaagagaat aaaaaaagca aagccaagga acctcctcct aaaaagacga agaaaaataa
     3541 tagcagcaac agcaatgtga gcaagaagga gccagcgccc atgaagagca agccccctcc
     3601 cacgtatgag tcggaggaag aggacaagtg caagcctatg tcctatgagg agaagcggca
     3661 gctcagcttg gacatcaaca agctccccgg cgagaagctg ggccgcgtgg tgcacatcat
     3721 ccagtcacgg gagccctccc tgaagaattc caaccccgac gagattgaaa tcgactttga
     3781 gaccctgaag ccgtccacac tgcgtgagct ggagcgctat gtcacctcct gtttgcggaa
     3841 gaaaaggaaa cctcaagctg agaaagttga tgtgattgcc ggctcctcca agatgaaggg
     3901 cttctcgtcc tcagagtcgg agagctccag tgagtccagc tcctctgaca gcgaagactc
     3961 cgaaacacct gctcctgagg cacccaagga aatcccacca gaagctgtga aggagtatgt
     4021 tgacatcatg gaatggctgg tggggactca cttggccact ggggagtcag atggaaaaca
     4081 agaggaagaa gggcagcagc aggaggagga agggatgtat ccagatccag gtctcctgag
     4141 ctacatcaat gagctgtgtt ctcagaaggt ctttgtctcc aaggtggagg ctgtcattca
     4201 ccctcaattt ctggcagatc tgctgtcccc agaaaaacag agagatccct tggccttaat
     4261 tgaggagcta gagcaagaag aaggactcac tcttgcccag ctggtccaga agcgactcat
     4321 ggccttggaa gaggaggaag atgcagaggc gcctccaagt ttcagtggcg ctcagttgga
     4381 ctcaagtcct tctggttctg ttgaggatga agatggggat ggctcgacct gatcatagtg
     4441 aggctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     4501 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     4561 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     4621 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4681 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     4741 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4801 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4861 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4921 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4981 ggttcacgta cctagaagtt cctattccga agttcctatt ctctagaaag tataggaact
     5041 tccttggcca aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     5101 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     5161 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     5221 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     5281 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     5341 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     5401 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     5461 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     5521 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     5581 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     5641 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     5701 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     5761 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     5821 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     5881 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     5941 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     6001 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     6061 gcaaaggaat agcacgtact acgagatttc gattccaccg ccgccttcta tgaaaggttg
     6121 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6181 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     6241 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6301 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     6361 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6421 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6481 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6541 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6601 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6661 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6721 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6781 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6841 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6901 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6961 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7021 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7081 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7141 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7201 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7261 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7321 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7381 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7441 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7501 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7561 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7621 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7681 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7741 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7801 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7861 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7921 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7981 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8041 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8101 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8161 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8221 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8281 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8341 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8401 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8461 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8521 cctgacgtc
//
