LOCUS       sequence-405172-       11004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid allows for inducible expression of BRD4-NUT(deltaMIN) 
            mutant fusion tagged with mNeonGreen, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11004)
  AUTHORS   Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
  TITLE     Molecular features driving condensate formation and gene expression 
            by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
  JOURNAL   Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
  PUBMED    37488172
REFERENCE   2  (bases 1 to 11004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-07-24"; volume: "13(1):11907. doi"; pages: " 
            10.1038/s41598-023-39102-9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(deltaMIN)
FEATURES             Location/Qualifiers
     source          1..11004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1058..1084
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1088..1792
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     primer_bind     complement(6956..6973)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6962..7186
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7319..7338)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7470..7517
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7525..8547
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7525..8547
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8677..8798
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8714..8733)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8768..8787
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8847..8863)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8847..8863)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8860..8882)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8871..8887
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8895..8901,8902..8919,8920..8925))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8940..8961
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9078..9095)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9249..9837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9329..9348)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10008..10799,10800..10868))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10631..10650
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10869..10973)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     1021 attctgcaga tatccagcac agtggcggcc gatgggaccg gctgccaagc gcgtgaaact
     1081 cgatggcgtg agcaagggcg aggaggataa catggcctct ctcccagcga cacatgagtt
     1141 acacatcttt ggctccatca acggtgtgga ctttgacatg gtgggtcagg gcaccggcaa
     1201 tccaaatgat ggttatgagg agttaaacct gaagtccacc aagggtgacc tccagttctc
     1261 cccctggatt ctggtccctc atatcgggta tggcttccat cagtacctgc cctaccctga
     1321 cgggatgtcg cctttccagg ccgcaatggt agatggctcc ggataccaag tccatcgcac
     1381 aatgcagttt gaagatggtg cctcccttac tgttaactac cgctacacct acgagggaag
     1441 ccacatcaaa ggagaggccc aggtgaaggg gactggtttc cctgctgacg gtcctgtgat
     1501 gaccaactcg ctgaccgctg cggactggtg caggtcgaag aagacttacc ccaacgacaa
     1561 aaccatcatc agtaccttta agtggagtta caccactgga aatggcaagc gctaccggag
     1621 cactgcgcgg accacctaca cctttgccaa gccaatggcg gctaactatc tgaagaacca
     1681 gccgatgtac gtgttccgta agacggaact caagcactcc aagaccgaac tcaacttcaa
     1741 ggagtggcaa aaggccttta ccgatgtgat gggcatggac gagctgtaca agggcggctc
     1801 gtacgtgtac aagtctgcgg agagcggccc tgggacgaga ttgagaaatc tgccagtaat
     1861 gggggatgga ctagaaactt cccaaatgtc tacaacacag gcccaggccc aaccccagcc
     1921 agccaacgca gccagcacca accccccgcc cccagagacc tccaacccta acaagcccaa
     1981 gaggcagacc aaccaactgc aatacctgct cagagtggtg ctcaagacac tatggaaaca
     2041 ccagtttgca tggcctttcc agcagcctgt ggatgccgtc aagctgaacc tccctgatta
     2101 ctataagatc attaaaacgc ctatggatat gggaacaata aagaagcgct tggaaaacaa
     2161 ctattactgg aatgctcagg aatgtatcca ggacttcaac actatgttta caaattgtta
     2221 catctacaac aagcctggag atgacatagt cttaatggca gaagctctgg aaaagctctt
     2281 cttgcaaaaa ataaatgagc tacccacaga agaaaccgag atcatgatag tccaggcaaa
     2341 aggaagagga cgtgggagga aagaaacagg gacagcaaaa cctggcgttt ccacggtacc
     2401 aaacacaact caagcatcga ctcctccgca gacccagacc cctcagccga atcctcctcc
     2461 tgtgcaggcc acgcctcacc ccttccctgc cgtcaccccg gacctcatcg tccagacccc
     2521 tgtcatgaca gtggtgcctc cccagccact gcagacgccc ccgccagtgc ccccccagcc
     2581 acaaccccca cccgctccag ctccccagcc cgtacagagc cacccaccca tcatcgcggc
     2641 caccccacag cctgtgaaga caaagaaggg agtgaagagg aaagcagaca ccaccacccc
     2701 caccaccatt gaccccattc acgagccacc ctcgctgccc ccggagccca agaccaccaa
     2761 gctgggccag cggcgggaga gcagccggcc tgtgaaacct ccaaagaagg acgtgcccga
     2821 ctctcagcag cacccagcac cagagaagag cagcaaggtc tcggagcagc tcaagtgctg
     2881 cagcggcatc ctcaaggaga tgtttgccaa gaagcacgcc gcctacgcct ggcccttcta
     2941 caagcctgtg gacgtggagg cactgggcct acacgactac tgtgacatca tcaagcaccc
     3001 catggacatg agcacaatca agtctaaact ggaggcccgt gagtaccgtg atgctcagga
     3061 gtttggtgct gacgtccgat tgatgttctc caactgctat aagtacaacc ctcctgacca
     3121 tgaggtggtg gccatggccc gcaagctcca ggatgtgttc gaaatgcgct ttgccaagat
     3181 gccggacgag cctgaggagc cagtggtggc cgtgtcctcc ccggcagtgc cccctcccac
     3241 caaggttgtg gccccgccct catccagcga cagcagcagc gatagctcct cggacagtga
     3301 cagttcgact gatgactctg aggaggagcg agcccagcgg ctggctgagc tccaggagca
     3361 gctcaaagcc gtgcacgagc agcttgcagc cctctctcag ccccagcaga acaaaccaaa
     3421 gaaaaaggag aaagacaaga aggaaaagaa aaaagaaaag cacaaaagga aagaggaagt
     3481 ggaagagaat aaaaaaagca aagccaagga acctcctcct aaaaagacga agaaaaataa
     3541 tagcagcaac agcaatgtga gcaagaagga gccagcgccc atgaagagca agccccctcc
     3601 cacgtatgag tcggaggaag aggacaagtg caagcctatg tcctatgagg agaagcggca
     3661 gctcagcttg gacatcaaca agctccccgg cgagaagctg ggccgcgtgg tgcacatcat
     3721 ccagtcacgg gagccctccc tgaagaattc caaccccgac gagattgaaa tcgactttga
     3781 gaccctgaag ccgtccacac tgcgtgagct ggagcgctat gtcacctcct gtttgcggaa
     3841 gaaaaggaaa cctcaagctg agaaagttga tgtgattgcc ggctcctcca agatgaaggg
     3901 cttctcgtcc tcagagtcgg agagctccag tgagtccagc tcctctgaca gcgaagactc
     3961 cgaaacagca tctgcattgc cgggaccgga tatgagcatg aaacctagtg ccgccctgtc
     4021 tccatcccct gcacttccct ttctcccacc aacttctgac ccaccagacc acccacccag
     4081 ggagccacct ccacagccca tcatgccttc agtattctct ccagacaacc ctctgatgct
     4141 ctctgctttc cccagctcac tgttggtgac aggggacggg ggcccttgcc tcagtggggc
     4201 tggggctggc aaggtcattg tcaaagtcaa gacagaaggg gggtcagctg agccctctca
     4261 aactcagaac tttatcctta ctcagactgc cctcaattcg actgccccgg gcactccctg
     4321 tggaggcctt gagggtcctg cacctccatt tgtgacagca tctaatgtga agaccattct
     4381 gccctctaag gctgttggtg tcagccagga gggtcctcca ggccttccgc ctcagcctcc
     4441 accaccagtt gctcaactgg tccccattgt gcccctggaa aaagcttggc cagggccaca
     4501 tgggacaacc ggggaaggag gtcctgtggc cactctatcc aagccttccc taggtgaccg
     4561 ctccaaaatt tccaaggacg tttatgagaa cttccgtcag tggcagcgtt acaaagcctt
     4621 ggcccggagg cacctatccc agagtcctga cacagaagct ctttcctgtt ttcttatccc
     4681 agtgcttcgt tccctggccc ggctgaagcc cactatgacc ctggaggagg gactgccatt
     4741 ggctgtgcag gagtgggagc acaccagcaa ctttgaccgg atgatctttt atgagatggc
     4801 agaaaggttc atggagtttg aggctgagga gatgcagatt cagaacacac agctgatgaa
     4861 tgggtctcag ggcctgtctc ctgcaacccc tttgaaactt gatcctctag ggcccctggc
     4921 ctctgaggtt tgccagcagc cagtgtacat tccgaagaag gcagcctcca agacacgggc
     4981 cccccgccgg cgtcagcgta aagcccagag acctgggcgg cttcggccct cacctgggct
     5041 tcagggggct gggggcgccg cttgccttgg aaaggtttct tcttcaggaa aacgggcaag
     5101 agaagtgcat ggtgggcagg agcaagccct agatagcccc agagggatgc acagggatgg
     5161 gaacactctg ccatccccca gcagctggga cctgcagcca gaacttgcag ctccacaggg
     5221 aactccggga cccttgggtg tggagaggag agggtctggg aaggttataa accaggtatc
     5281 tctacatcag gatggccatc taggaggcgc tgggcctcct gggcactgcc tggtggctga
     5341 taggacttca gaggctctgc ccctttgttg gcagggaggc ttccagcctg agagcactcc
     5401 cagtttggat gctggacttg cagagctggc tcctctgcaa ggacaagggt tagaaaagca
     5461 agtcctggga ttgcagaaag gacaacaaac agggggtcgt ggagtgcttc ctcaagggaa
     5521 ggagccttta gcagtgccct gggaaggctc ttcaggagcc atgtggggag atgacagagg
     5581 tacccccatg gctcagagtt atgatcagaa tccttcccct agagcagctg gggagaggga
     5641 cgatgtctgt ctcagcccag gagtttggct gagcagtgag atggatgctg taggcttgga
     5701 gctgcctgta caaatagagg aggtcataga gagcttccaa gttgagaagt gtgtaactga
     5761 gtatcaggaa ggctgccagg gactgggctc caggggcaac atttccctgg gtcctggaga
     5821 aaccctagta cctggggata cggagagcag tgtgattccc tgtggaggca cagttgcggc
     5881 agctgcccta gaaaagagaa actattgcag cttgccagga cctttgaggg ccaacagccc
     5941 acccttgagg tccaaagaaa atcaagaaca gagctgtgaa accgtagggc atcccagtga
     6001 tctgtgggca gaaggttgct tcccattgct agaaagtggt gattccacac tggggtcttc
     6061 caaagaaacc cttccaccca catgccaagg caatctcctt atcatgggga ctgaggatgc
     6121 ctcctccttg cctgaagcca gccaagaggc agggagcaga ggcaattcct tttctcctct
     6181 gttggaaacc atagaacctg tcaacatact agatgttaaa gatgactgtg gcctccaact
     6241 aagggtcagc gaggacacct gcccactgaa tgttcattct tatgaccccc aaggagaagg
     6301 cagggtggat cctgatctgt ccaagcctaa aaaccttgct cctttacaag agagtcagga
     6361 gtcttacaca actgggactc ccaaagcaac atcttctcac cagggccttg gaagcacttt
     6421 gcctagaagg ggaaccagga atgccatagt tccgagagaa acttctgtta gtaaaacaca
     6481 caggtcagca gacagggcca aaggaaagga gaaaaagaaa aaggaagcag aggaagagga
     6541 tgaggaactc tccaactttg cttacctctt ggcctctaaa cttagcctct caccaaggga
     6601 gcatcccctc agtcctcacc atgcctcagg aggtcagggc agccagagag catcccacct
     6661 gctccctgct ggagcaaaag gccccagcaa acttccatat cctgttgcca agtctgggaa
     6721 gcgagctcta gctggaggtc cagcccctac tgaaaagaca ccccactcag gagctcaact
     6781 tggggtcccc agggagaaac ccctagctct gggagtagtt cgaccctcac agcctcgtaa
     6841 aaggcggtgt gacagttttg tcacgggcag aaggaagaaa cgacgtcgta gccagggctc
     6901 gacctgatca tagtgaggct cgagtctaga gggcccgttt aaacccgctg atcagcctcg
     6961 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     7021 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     7081 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     7141 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     7201 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     7261 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     7321 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     7381 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     7441 cttgattagg gtgatggttc acgtacctag aagttcctat tccgaagttc ctattctcta
     7501 gaaagtatag gaacttcctt ggccaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     7561 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     7621 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     7681 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     7741 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
     7801 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
     7861 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
     7921 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     7981 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     8041 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     8101 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     8161 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     8221 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     8281 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
     8341 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     8401 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     8461 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     8521 agcactcgtc cgagggcaaa ggaatagcac gtactacgag atttcgattc caccgccgcc
     8581 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     8641 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     8701 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8761 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     8821 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8881 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     8941 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     9001 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     9061 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     9121 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     9181 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     9241 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9301 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9361 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     9421 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     9481 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     9541 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9601 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9661 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9721 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9781 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9841 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9901 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9961 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    10021 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    10081 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    10141 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    10201 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    10261 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    10321 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    10381 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10441 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10501 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10561 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10621 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10681 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10741 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10801 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10861 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10921 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10981 ccccgaaaag tgccacctga cgtc
//
