LOCUS       sequence-405175-        1734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the Hro ES3 insert with a chloramphenicol
            resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo22
FEATURES             Location/Qualifiers
     source          1..1734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      117..144
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(165..824)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGE"
     primer_bind     739..758
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(825..927)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     oriT            1087..1196
                     /note="incP origin of transfer"
     rep_origin      1311..1699
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 aatacattta aaaaataaag tatacatata aaaacctatt cagtagaatc agccgcatca
       61 ggcaacgacc attcaatcaa aaagaggagg caggcaacat tacgagacct cacactggct
      121 caccttcggg tgggcctttc tgcgtttata cctaggagtc aaaattactc cccgccctgc
      181 cactcatcgc agtactgttg taattcatta agcattctac cgacatggaa gccatcacaa
      241 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      301 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
      361 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
      421 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
      481 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
      541 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
      601 atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
      661 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
      721 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
      781 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
      841 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
      901 aagttggaac ctcttacgtg ccgatcagtc aattcgaggg ggatcaattc cgttttccgc
      961 tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc
     1021 acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg
     1081 tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt
     1141 cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccggctac
     1201 cgccggcgta acagatgagg gcaagcggat ggctgatgaa accaagccaa ccaggaaggg
     1261 cagcccacct atcacggaat tgatccggcc acgatgcgtc cggcgtagag gatctgaaga
     1321 tcagcagttc aacctgttga tagtacgtac taagctctca tgtttcacgt actaagctct
     1381 catgtttaac gtactaagct ctcatgttta acgaactaaa ccctcatggc taacgtacta
     1441 agctctcatg gctaacgtac taagctctca tgtttcacgt actaagctct catgtttgaa
     1501 caataaaatt aatataaatc agcaacttaa atagcctcta aggttttaag ttttataaga
     1561 aaaaaaagaa tatataaggc ttttaaagct tttaaggttt aacggttgtg gacaacaagc
     1621 cagggatgta acgcactgag aagcccttag agcctctcaa agcaattttg agtgacacag
     1681 gaacacttaa cggctgacat gggggtctcg gagacatcta tatatttaat tatg
//
