LOCUS       sequence-405178-        1820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the Bpi ES1 insert with a chloramphenicol
            resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo26
FEATURES             Location/Qualifiers
     source          1..1820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      25..52
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(73..732)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGE"
     primer_bind     647..666
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(733..835)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      894..1282
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 tttcgaatta cgagacctca cactggctca ccttcgggtg ggcctttctg cgtttatacc
       61 taggagtcaa aattactccc cgccctgcca ctcatcgcag tactgttgta attcattaag
      121 cattctaccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
      181 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
      241 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
      301 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
      361 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
      421 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
      481 tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg
      541 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
      601 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
      661 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
      721 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
      781 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcagtcaa
      841 ttcgaggggg atcaattccg tattgatccg gccacgatgc gtccggcgta gaggatctga
      901 agatcagcag ttcaacctgt tgatagtacg tactaagctc tcatgtttca cgtactaagc
      961 tctcatgttt aacgtactaa gctctcatgt ttaacgaact aaaccctcat ggctaacgta
     1021 ctaagctctc atggctaacg tactaagctc tcatgtttca cgtactaagc tctcatgttt
     1081 gaacaataaa attaatataa atcagcaact taaatagcct ctaaggtttt aagttttata
     1141 agaaaaaaaa gaatatataa ggcttttaaa gcttttaagg tttaacggtt gtggacaaca
     1201 agccagggat gtaacgcact gagaagccct tagagcctct caaagcaatt ttgagtgaca
     1261 caggaacact taacggctga catgggggtc tcggagactg gcacctggaa caagctcaat
     1321 cgcttcgatg ctgcctatct cgccttctcg caagcctatg ggttcatgtc gaaccgcatc
     1381 attgcttata ttccgggcct tgttccctat atgagtggcg ctgtcagctt ccgtaattat
     1441 gtgactggcg cggaactggt tgcagccatg atcaatgatt gctcatggtt tattcaggtc
     1501 atgccggcca ttgccaatct tcgggcgaat gccggtcgtg tgacgggtct tgctcaggca
     1561 atcgacagtg ttcaggagcc ggccgcatta cagaacaagg caaactcaac cgtccataaa
     1621 cacaacgcgg cgcttttgaa aaagtggctt tgaaactcta tactaagcca agtaataagc
     1681 ttgtaacggg tcgttgacgt cactactgcc agtgaagcgc gcagtgcata tggttattct
     1741 tgttcagaat cgctcaatct cctcttgggc ggtttcgaat ttttgattat gaatgggctt
     1801 atatcattat tgaaggtgtg
//
