LOCUS       sequence-405198-        1787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the Sma ES2 insert with a chloramphenicol
            resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo35
FEATURES             Location/Qualifiers
     source          1..1787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(20..122)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      181..569
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      1099..1126
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(1147..1787,1..19))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGE"
     primer_bind     1721..1740
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
       61 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat
      121 cagtcaattc gagggggatc aattccgtat tgatccggcc acgatgcgtc cggcgtagag
      181 gatctgaaga tcagcagttc aacctgttga tagtacgtac taagctctca tgtttcacgt
      241 actaagctct catgtttaac gtactaagct ctcatgttta acgaactaaa ccctcatggc
      301 taacgtacta agctctcatg gctaacgtac taagctctca tgtttcacgt actaagctct
      361 catgtttgaa caataaaatt aatataaatc agcaacttaa atagcctcta aggttttaag
      421 ttttataaga aaaaaaagaa tatataaggc ttttaaagct tttaaggttt aacggttgtg
      481 gacaacaagc cagggatgta acgcactgag aagcccttag agcctctcaa agcaattttg
      541 agtgacacag gaacacttaa cggctgacat gggggtctcg gagacgattc cgaggccgaa
      601 gccatcctga atcgcgttca ggaaggtgtc gagaagccgc gtccgaaggt gctgttcgag
      661 ccgggccaga tggttcgcgt caccgacggc ccgttcaacg atttcaatgg cgtcgtcgaa
      721 gaagtcaact acgagaagag ccgtctgcgc gtctcggtgc tgatcttcgg tcgtgccact
      781 ccggtcgagc tcgagttcgg ccaggtcgaa aaggccgtct gaccggccct gtagagtcga
      841 gccatgctcg actcccgacc agaaaccggg ccttgcccgg tttcgttcgt ttatatgccg
      901 cttcaggcat gtgaagaaaa aacctgttat agtgcgcggc tccccgctgg gaagccagca
      961 ggacgaggcc gccgcaaggt ggccttcagc atgatttcaa aacgcgatgc cgggacgcgt
     1021 gattcccggt ccgatgggga gcctgttgtc gcaaggcgct agcacccgga gagcatactc
     1081 attacgagac ctcacactgg ctcaccttcg ggtgggcctt tctgcgttta tacctaggag
     1141 tcaaaattac tccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     1201 accgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1261 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1321 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     1381 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1441 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     1501 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     1561 cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag
     1621 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     1681 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     1741 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatc
//
