LOCUS       sequence-405245-        1613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the Bpi ES7 insert with a chloramphenicol
            resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo32
FEATURES             Location/Qualifiers
     source          1..1613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      175..202
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(223..882)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGE"
     primer_bind     797..816
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(883..985)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      1044..1432
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 atagttacag cggttccaac gattcagcct taactggaac cgctgtaagc ctctcccaat
       61 ttttatatat tttatctatt tctcacttgc caaacaccat caatccgatg attttaaata
      121 gtcgaatcgt aattttatct ataatttgag gaagccatta cgagacctca cactggctca
      181 ccttcgggtg ggcctttctg cgtttatacc taggagtcaa aattactccc cgccctgcca
      241 ctcatcgcag tactgttgta attcattaag cattctaccg acatggaagc catcacaaac
      301 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
      361 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
      421 ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
      481 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
      541 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
      601 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
      661 acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
      721 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
      781 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
      841 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
      901 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
      961 gttggaacct cttacgtgcc gatcagtcaa ttcgaggggg atcaattccg tattgatccg
     1021 gccacgatgc gtccggcgta gaggatctga agatcagcag ttcaacctgt tgatagtacg
     1081 tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt
     1141 ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc
     1201 tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact
     1261 taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa
     1321 gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct
     1381 tagagcctct caaagcaatt ttgagtgaca caggaacact taacggctga catgggggtc
     1441 tcggagactc ttaagatgac catgcgagct gccgcacatg cagctcgcgc gcctgcggct
     1501 gctttgattt taaagcattt ccagcaaaag tgggaacctc ctccgcaggg aaatcagttc
     1561 gccggactga tttcttttcc tgctgcgatg cgtcggataa tgcgtacaaa caa
//
