LOCUS       sequence-405249-        1427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the Sma ES1 insert with a chloramphenicol
            resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo34
FEATURES             Location/Qualifiers
     source          1..1427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     546..565
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(632..734)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      793..1181
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      1351..1378
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(1399..1427,1..631))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGE"
ORIGIN
        1 actgttgtaa ttcattaagc attctaccga catggaagcc atcacaaacg gcatgatgaa
       61 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
      121 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
      181 cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
      241 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
      301 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
      361 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cggaattccg
      421 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
      481 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
      541 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
      601 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
      661 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
      721 ttacgtgccg atcagtcaat tcgaggggga tcaattccgt attgatccgg ccacgatgcg
      781 tccggcgtag aggatctgaa gatcagcagt tcaacctgtt gatagtacgt actaagctct
      841 catgtttcac gtactaagct ctcatgttta acgtactaag ctctcatgtt taacgaacta
      901 aaccctcatg gctaacgtac taagctctca tggctaacgt actaagctct catgtttcac
      961 gtactaagct ctcatgtttg aacaataaaa ttaatataaa tcagcaactt aaatagcctc
     1021 taaggtttta agttttataa gaaaaaaaag aatatataag gcttttaaag cttttaaggt
     1081 ttaacggttg tggacaacaa gccagggatg taacgcactg agaagccctt agagcctctc
     1141 aaagcaattt tgagtgacac aggaacactt aacggctgac atgggggtct cggagacggt
     1201 tttgcctgta tcatgcccgg ctcgacccac ccaggacggg tcgtacgccg atcatggcag
     1261 gacggaaaga gcggcccagg ccgccgccag atcagtcaga aaccagggtc atatcccatc
     1321 aaggacattg caattacgag acctcacact ggctcacctt cgggtgggcc tttctgcgtt
     1381 tatacctagg agtcaaaatt actccccgcc ctgccactca tcgcagt
//
