LOCUS       sequence-405255-        9976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This vector encodes of a synCAM (iCAM1) and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Stevens AJ, Harris AR, Gerdts J, Kim KH, Trentesaux C, Ramirez JT, 
            McKeithan WL, Fattahi F, Klein OD, Fletcher DA, Lim WA
  TITLE     Programming multicellular assembly with synthetic cell adhesion 
            molecules.
  JOURNAL   Nature. 2023 Feb;614(7946):144-152. doi: 10.1038/s41586-022-05622-z.
            Epub 2022 Dec 12.
  PUBMED    36509107
REFERENCE   2  (bases 1 to 9976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-05622-z"; journalName: "Nature"; date: 
            "2023-02"; volume: "614"; issue: "7946"; pages: "144-152"
COMMENT     Sequence Label: pHR-flag-GFP-iCAM-1
FEATURES             Location/Qualifiers
     source          1..9976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..144
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(214..231)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(385..973)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(465..484)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1144..1935,1936..2004))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1767..1786
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2005..2109)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2177..2195
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2233..2255)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2355..2374
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2588..2607
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2897..2915
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2897..2914
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             2940..3173
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     3263..3279
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3264..3280
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3283..3871
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3389..3400
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3798..3818
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(4146..4856,4857..4859,4860..4862))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(4869..4892)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(4984..5391)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    5544..5661
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5946..5990)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6175..6408
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6905..7030
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7077..7710
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7878..8012
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7902..7921)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7956..7975
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8437..8457)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8587..8652
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(join(9786..9976,1..139))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9800..9935
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg
       61 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
      121 cacaccctaa ctgacacaca ttccacagct gcattaatga atcggccaac gcgcggggag
      181 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      241 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      301 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      361 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      421 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      481 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      541 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      601 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      661 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      721 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
      781 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
      841 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
      901 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
      961 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1021 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1081 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1141 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     1201 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     1261 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     1321 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     1381 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     1441 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     1501 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     1561 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     1621 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     1681 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     1741 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     1801 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     1861 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     1921 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     1981 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     2041 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     2101 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     2161 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     2221 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     2281 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     2341 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccatt cgacgctctc
     2401 ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg
     2461 ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc
     2521 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
     2581 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
     2641 cggccacgat gcgtccggcg tagaggatct ggctagcgat gaccctgctg attggttcgc
     2701 tgaccatttc cgggtgcggg acggcgttac cagaaactca gaaggttcgt ccaaccaaac
     2761 cgactctgac ggcagtttac gagagagatg atagggtctg cttcagtaag ccagatgcta
     2821 cacaattagg cttgtacata ttgtcgttag aacgcggcta caattaatac ataaccttat
     2881 gtatcataca catacgattt aggtgacact atagaataca cggaattaat tctagatgct
     2941 gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca
     3001 caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag
     3061 tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc
     3121 agtacaagca aaaagcagat cttgtcttcg ttgggagtga attagccctt ccagtccccc
     3181 cttttctttt aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg
     3241 taccgagctc gaattaattc cctcgaggtc gacggtatcg atgcggggag gcggcccaaa
     3301 gggagatccg actcgtctga gggcgaaggc gaagacgcgg aagaggccgc agagccggca
     3361 gcaggccgcg ggaaggaagg tccgctggat tgagggccga agggacgtag cagaaggacg
     3421 tcccgcgcag aatccaggtg gcaacacagg cgagcagcca aggaaaggac gatgatttcc
     3481 ccgacaacac cacggaattg tcagtgccca acagccgagc ccctgtccag cagcgggcaa
     3541 ggcaggcggc gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa
     3601 ggagctgaca ggtggtggca atgccccaac cagtgggggt tgcgtcagca aacacagtgc
     3661 acaccacgcc acgttgcctg acaacgggcc acaactcctc ataaagagac agcaaccagg
     3721 atttatacaa ggaggagaaa atgaaagcca tacgggaagc aatagcatga tacaaaggca
     3781 ttaaagcagc gtatccacat agcgtaaaag gagcaacata gttaagaata ccagtcaatc
     3841 tttcacaaat tttgtaatcc agaggttgat tatcgataag cttgatatca agcttgcatg
     3901 cctgcaggtc gactctagag tcgcggcctc agtcagtcac gcatgttgca ggtgggagtt
     3961 gcggccgcaa gtcaaggatc ttacggtggt gtagcctgag tatttggctt cattggagtc
     4021 cccttttggg cctgttggag cctatacttt tttatcttgc gttggcggtt atacaggtag
     4081 gtagaaaggc cagccgtccc cataatgaca gccgccgcaa cgaccgttat aatcactatg
     4141 gatcccttat acagctcatc cattcccagg gtgatcccag cagccgtcac gaattcaagc
     4201 agaaccatat ggtctctttt ttcgttagga tccttggaga gggctgattg cgtggagagg
     4261 tagtggttat ctggcagcaa aaccggaccg tcgccgatag gcgtgttttg ctggtaatgg
     4321 tcggccagtt gcacagagcc atcctcgata ttgtgtcgga ttttgaagtt caccttgatg
     4381 ccgttcttct gcttatcagc cataatatac acgttgtggc tgttgtagtt gtactccagc
     4441 ttgtggccca aaatattgcc gtcttctttg aaatcgattc ctttcaactc aattctattt
     4501 accaacgtgt ccccctcgaa cttaacttca gccctggttt tatagttacc gtcgtccttg
     4561 aagaatatgg tgcgttcctg aacatagccc tcaggcatcg cggacttgaa gaaatcgtgc
     4621 tgtttcatgt ggtcggggta ccgggagaag cactggactc cgtaggtgag ggtggtcacg
     4681 agtgtaggcc agggcactgg cagctttcct gttgtacaga tgaacttcaa cgtcagttta
     4741 ccgtacgtag cgtcgccctc tccttcgccg ctcactgaga acttgtgtcc gttaacgtct
     4801 ccgtccagct caaccaggat gggcacaact ccggtgaaca attcttcacc cttagaaacc
     4861 atggatcctt tatcatcgtc atctttataa tccgcaaaaa caagacaaaa aatgtaactg
     4921 agggcgataa ttgtcttcat ggtggcggcg acgcgtgaga attctcgaga gctccagatc
     4981 ccccccgggc gactcagtct gtcggaggac tggcgcgccg agtgaggggt tgtgagctct
     5041 tttatagagc tcgggaagca gaagcgcgcg aacagaagcg agaagcaggc tgattggtta
     5101 attcaaataa ggcgcagggt catttcaggt ccttggggga gcctggaaac atctgatggg
     5161 tcttaagaaa ctgctgaggg ttgggccata tctggggacc atctgttctt ggcctcgggc
     5221 cggggccgaa actgcggtga ccatctgttc ttggccccgg gccggggccg aaactgctca
     5281 ccgcagatat cctgtttggc ccaacgttag ctattttcat gtacccgccc ttgatctgaa
     5341 cttctctatt cttggtttgg tatttttcca tgccttgcaa aatggcgtta ctgcagctag
     5401 cttgccaaac ctacaggtgg ggtctttcat tccccccttt ttctggagac taaataaaat
     5461 cttttatttt atcggggctg caggaattcg atatcaagct tatcgatcca aactggatct
     5521 ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg taatttgttt ttgtaattct
     5581 ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact gtacccccca
     5641 atcccccctt ttcttttaaa attgtggatg aatactgcca tttggcgata ccgtcgagat
     5701 ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct
     5761 tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat
     5821 ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag
     5881 cctcctacta tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa
     5941 ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg
     6001 ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc
     6061 cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca
     6121 ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat ccccaggagc
     6181 tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc
     6241 cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa attgttctgc
     6301 tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg
     6361 cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat tcccactgct
     6421 cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc taatggttca
     6481 atttttacta ctttatattt atataattca cttctccaat tgtccctcat atctcctcct
     6541 ccaggtctga agatcagcgg ccggccgctt gctgtgcggt ggtcttactt ttgttttgct
     6601 cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt tgatgcacac
     6661 aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct gtctgaaggg
     6721 atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac aggccaggat
     6781 taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat ttatattttt
     6841 tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc ccccgcttaa
     6901 tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa atttttggcg
     6961 tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc
     7021 gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg cgccactgct
     7081 agagattttc cacactgact aaaagggtct gagggatctc tagttaccag agtcacacaa
     7141 cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct taagcagtgg
     7201 gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga gagacccagt
     7261 acaggcaaaa agcagctgct tatatgcagg atctgagggc tcgccactcc ccagtcccgc
     7321 ccaggccacg cctccctgga aagtccccag cggaaagtcc cttgtagcaa gctcgatatc
     7381 agcagttctt gaagtactcc ggatgcagct ctcgggccac gtgatgaaat gctaggcggc
     7441 tgtcaaacct ccactctaac acttctctct ccgggtcatc catcccatgc aggctcacag
     7501 ggtgtaacaa gctggtgttc tctcctttat tggcctcttc taccttatct ggctcaactg
     7561 gtactagctt gtagcaccat ccaaaggtca gtggatatct gacccctggc cctggtgtgt
     7621 agttctgcta atcagggaag tagccttgtg tgtggtagat ccacagatca aggatatctt
     7681 gtcttctttg ggagtgaatt agcccttcca actactaagt ttgtagtaca tatttaacaa
     7741 atacaatttc tttaaaatga aaataattca gaggaatcac aggtttagag taaatgaaac
     7801 cacaggtaat tggcagtggt aatagggtat ggggtgggaa gtttgggatg attttggtta
     7861 gcttgagtta tccagttgat ccagacatga taagatacat tgatgagttt ggacaaacca
     7921 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     7981 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     8041 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     8101 gtatggctga ttatgatctc tagtcaaggc actatacatc aaatattcct tattaacccc
     8161 tttacaaatt aaaaagctaa aggtacacaa tttttgagca tagttattaa tagcagacac
     8221 tctatgcctg tgtggagtaa gaaaaaacag tatgttatga ttataactgt tatgcctact
     8281 tataaaggtt acagaatatt tttccataat tttcttgtat agcagtgcag ctttttcctt
     8341 tgtggtgtaa atagcaaagc aagcaagagt tctattacta aacacagcat gactcaaaaa
     8401 acttagcaat tctgaaggaa agtccttggg gtcttctacc tttctcttct tttttggagg
     8461 agtagaatgt tgagagtcag cagtagcctc atcatcacta gatggcattt cttctgagca
     8521 aaacaggttt tcctcattaa aggcattcca ccactgctcc cattcatcag ttccataggt
     8581 tggaatctaa aatacacaaa caattagaat cagtagttta acacattata cacttaaaaa
     8641 ttttatattt accttagagc tttaaatctc tgtaggtagt ttgtccaatt atgtcacacc
     8701 acagaagtaa ggttccttca caaagatccg gggcccactc ataaatccag ttgccgccac
     8761 ggtagccaat caccgtatcg tataaatcat cgtcggtacg ttcggcatcg ctcatcacaa
     8821 tacgtgcctg gacgtcgagg atttcgcgtg ggtcaatgcc gcgccagatc cacatcagac
     8881 ggttaatcat gcgataccag tgagggatgg ttttaccatc aagggccgac tgcacaggcg
     8941 gttgtgcgcc gtgattaaag cggcggacta gcgtcgaggt ttcaggatgt ttaaagcggg
     9001 gtttgaacag ggtttcgctc aggtttgcct gtgtcatgga tgcagcctcc agaatactta
     9061 ctggaaacta ttgtaacccg cctgaagtta aaaagaacaa cgcccggcag tgccaggcgt
     9121 tgaaaagatt agcgaccgga gattggcggg acgaatacga cgcccatatc ccacggctgt
     9181 tcaatccagg tatcttgcgg gatatcaaca acatagtcat caaccagcgg acgaccagcc
     9241 ggttttgcga agatggtgac aaagtgcgct tttggataca tttcacgaat cgcaaccgca
     9301 gtaccaccgg tatccaccag gtcatcaata acgatgaagc cttcgccatc gccttctgcg
     9361 cgtttcagca ctttaagctc gcgctggttg tcgtgatcgt agctggaaat acaaacggta
     9421 tcgacatgac gaatacccag ttcacgcgcc agtaacgcac ccggtacaga ccgccacggc
     9481 ttacggcaat aatgcctttc cattgttcag aaggcatcag tcggcttgcg agtttacgtg
     9541 catggatctg caacatgtcc caggtgacga tgtatttttc gctcatgtga agtgtcccag
     9601 cctgtttatc tacggcttaa aaagtgttcg aggggaaaat aggttgcgcg agattataga
     9661 gatctggcgc actaaaaacc agtatttcac atgagtccgc gtctttttac gcactgcctc
     9721 tccctgacgc gggataaagt ggtattctca aacatatctc gcaagcctgt cttgtgtcca
     9781 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     9841 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     9901 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     9961 gttgctgact aattga
//
