LOCUS       sequence-405260-       10372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This vector encodes of a synCAM (Ecad) and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Stevens AJ, Harris AR, Gerdts J, Kim KH, Trentesaux C, Ramirez JT, 
            McKeithan WL, Fattahi F, Klein OD, Fletcher DA, Lim WA
  TITLE     Programming multicellular assembly with synthetic cell adhesion 
            molecules.
  JOURNAL   Nature. 2023 Feb;614(7946):144-152. doi: 10.1038/s41586-022-05622-z.
            Epub 2022 Dec 12.
  PUBMED    36509107
REFERENCE   2  (bases 1 to 10372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-05622-z"; journalName: "Nature"; date: 
            "2023-02"; volume: "614"; issue: "7946"; pages: "144-152"
COMMENT     Sequence Label: pHR-flag-GFP-Ecad
FEATURES             Location/Qualifiers
     source          1..10372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     207..227
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(297..314)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(468..1056)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(548..567)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1227..2018,2019..2087))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1850..1869
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2088..2192)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2260..2278
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2316..2338)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2438..2457
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2671..2690
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2980..2998
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2980..2997
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             3023..3256
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     3346..3362
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3347..3363
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3366..3954
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3472..3483
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3881..3901
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(4625..5335,5336..5338,5339..5341))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(5348..5371)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(5463..5870)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    6023..6140
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6425..6469)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6654..6887
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7384..7509
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7556..8189
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8357..8491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8381..8400)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8435..8454
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8916..8936)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          9066..9131
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(join(10265..10372,1..222))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10279..10372,1..42)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg
       61 gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
      121 tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc
      181 tgctggggag cctggggact ttccacaccc taactgacac acattccaca gctgcattaa
      241 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
      301 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      361 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      421 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      481 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      541 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      601 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      661 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      721 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      781 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      841 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      901 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      961 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1021 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1081 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1141 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1201 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     1261 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     1321 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     1381 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     1441 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1501 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1561 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1621 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     1681 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1741 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1801 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1861 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1921 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1981 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2041 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2101 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2161 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     2221 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     2281 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     2341 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     2401 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     2461 agagtgcacc attcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag
     2521 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc
     2581 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     2641 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     2701 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctggctagc
     2761 gatgaccctg ctgattggtt cgctgaccat ttccgggtgc gggacggcgt taccagaaac
     2821 tcagaaggtt cgtccaacca aaccgactct gacggcagtt tacgagagag atgatagggt
     2881 ctgcttcagt aagccagatg ctacacaatt aggcttgtac atattgtcgt tagaacgcgg
     2941 ctacaattaa tacataacct tatgtatcat acacatacga tttaggtgac actatagaat
     3001 acacggaatt aattctagat gctgctagag attttccaca ctgactaaaa gggtctgagg
     3061 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     3121 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     3181 atctggtcta accagagaga cccagtacaa gcaaaaagca gatcttgtct tcgttgggag
     3241 tgaattagcc cttccagtcc ccccttttct tttaaaaagt ggctaagatc tacagctgcc
     3301 ttgtaagtca ttggtcttaa aggtaccgag ctcgaattaa ttccctcgag gtcgacggta
     3361 tcgatgcggg gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg
     3421 cggaagaggc cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc
     3481 cgaagggacg tagcagaagg acgtcccgcg cagaatccag gtggcaacac aggcgagcag
     3541 ccaaggaaag gacgatgatt tccccgacaa caccacggaa ttgtcagtgc ccaacagccg
     3601 agcccctgtc cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg caatagggag
     3661 ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg
     3721 ggttgcgtca gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc
     3781 ctcataaaga gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga
     3841 agcaatagca tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac
     3901 atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt gattatcgat
     3961 aagcttgata tcaagcttgc atgcctgcag gtcgactcta gagtcgcggc ctcagtcagt
     4021 cacgcatgtt gcaggtggga gttgcggccg caagtcaagg atccactgtc cacctgcctt
     4081 gctccacttg acgcgtttag tcgtcctcgc cgcctccgta catgtcagcc agcttcttga
     4141 agcgattgcc ccattcgttc aagtagtcat agtcctggtc tttgtctgac tctgaggagt
     4201 tcagggagct cagactagca gcttcggaac cgcttccttc atagtcaaac acgagcagag
     4261 aatcataagg cggggctgtg gggtcagtat cagccgcttt cagattttca tcaataaaat
     4321 ttccaatttc atcgggattg gcagggcggg gaagataccg ggggacactc atgagggttg
     4381 gtgcaacgtc gttacgagtc acttcaggcc gagcgtccag gcccctgtgc agctggctca
     4441 agtcaaagtc ctggtcctct tctccgcctc cttcttcatc atagtaataa acgttgtccc
     4501 gggtgtcatc ctctgggggc agtaagggct ctttgaccac cgctctcctc cgaagaaaca
     4561 gcaagagcag cagaatcaga attagcaaag caagaattcc tccaagaatc cccagaatgg
     4621 atcccttata cagctcatcc attcccaggg tgatcccagc agccgtcacg aattcaagca
     4681 gaaccatatg gtctcttttt tcgttaggat ccttggagag ggctgattgc gtggagaggt
     4741 agtggttatc tggcagcaaa accggaccgt cgccgatagg cgtgttttgc tggtaatggt
     4801 cggccagttg cacagagcca tcctcgatat tgtgtcggat tttgaagttc accttgatgc
     4861 cgttcttctg cttatcagcc ataatataca cgttgtggct gttgtagttg tactccagct
     4921 tgtggcccaa aatattgccg tcttctttga aatcgattcc tttcaactca attctattta
     4981 ccaacgtgtc cccctcgaac ttaacttcag ccctggtttt atagttaccg tcgtccttga
     5041 agaatatggt gcgttcctga acatagccct caggcatcgc ggacttgaag aaatcgtgct
     5101 gtttcatgtg gtcggggtac cgggagaagc actggactcc gtaggtgagg gtggtcacga
     5161 gtgtaggcca gggcactggc agctttcctg ttgtacagat gaacttcaac gtcagtttac
     5221 cgtacgtagc gtcgccctct ccttcgccgc tcactgagaa cttgtgtccg ttaacgtctc
     5281 cgtccagctc aaccaggatg ggcacaactc cggtgaacaa ttcttcaccc ttagaaacca
     5341 tggatccttt atcatcgtca tctttataat ccgcaaaaac aagacaaaaa atgtaactga
     5401 gggcgataat tgtcttcatg gtggcggcga cgcgtgagaa ttctcgagag ctccagatcc
     5461 cccccgggcg actcagtctg tcggaggact ggcgcgccga gtgaggggtt gtgagctctt
     5521 ttatagagct cgggaagcag aagcgcgcga acagaagcga gaagcaggct gattggttaa
     5581 ttcaaataag gcgcagggtc atttcaggtc cttgggggag cctggaaaca tctgatgggt
     5641 cttaagaaac tgctgagggt tgggccatat ctggggacca tctgttcttg gcctcgggcc
     5701 ggggccgaaa ctgcggtgac catctgttct tggccccggg ccggggccga aactgctcac
     5761 cgcagatatc ctgtttggcc caacgttagc tattttcatg tacccgccct tgatctgaac
     5821 ttctctattc ttggtttggt atttttccat gccttgcaaa atggcgttac tgcagctagc
     5881 ttgccaaacc tacaggtggg gtctttcatt cccccctttt tctggagact aaataaaatc
     5941 ttttatttta tcggggctgc aggaattcga tatcaagctt atcgatccaa actggatctc
     6001 tgctgtccct gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt
     6061 tagtttgtat gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa
     6121 tccccccttt tcttttaaaa ttgtggatga atactgccat ttggcgatac cgtcgagatc
     6181 cgttcactaa tcgaatggat ctgtctctgt ctctctctcc accttcttct tctattcctt
     6241 cgggcctgtc gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc
     6301 cctgcctaac tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc
     6361 ctcctactat cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat
     6421 tccacaaact tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt
     6481 tttgcgattc ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc
     6541 actccatcca ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat
     6601 tccaaggcac agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct
     6661 gttgatcctt taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc
     6721 agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct
     6781 gctgcactat accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc
     6841 ccatagtgct tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc
     6901 ttttttctct ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa
     6961 tttttactac tttatattta tataattcac ttctccaatt gtccctcata tctcctcctc
     7021 caggtctgaa gatcagcggc cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc
     7081 ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca
     7141 atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga
     7201 tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccaggatt
     7261 aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt
     7321 ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat
     7381 actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt
     7441 actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg
     7501 tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta
     7561 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     7621 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     7681 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
     7741 caggcaaaaa gcagctgctt atatgcagga tctgagggct cgccactccc cagtcccgcc
     7801 caggccacgc ctccctggaa agtccccagc ggaaagtccc ttgtagcaag ctcgatatca
     7861 gcagttcttg aagtactccg gatgcagctc tcgggccacg tgatgaaatg ctaggcggct
     7921 gtcaaacctc cactctaaca cttctctctc cgggtcatcc atcccatgca ggctcacagg
     7981 gtgtaacaag ctggtgttct ctcctttatt ggcctcttct accttatctg gctcaactgg
     8041 tactagcttg tagcaccatc caaaggtcag tggatatctg acccctggcc ctggtgtgta
     8101 gttctgctaa tcagggaagt agccttgtgt gtggtagatc cacagatcaa ggatatcttg
     8161 tcttctttgg gagtgaatta gcccttccaa ctactaagtt tgtagtacat atttaacaaa
     8221 tacaatttct ttaaaatgaa aataattcag aggaatcaca ggtttagagt aaatgaaacc
     8281 acaggtaatt ggcagtggta atagggtatg gggtgggaag tttgggatga ttttggttag
     8341 cttgagttat ccagttgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     8401 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     8461 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     8521 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     8581 tatggctgat tatgatctct agtcaaggca ctatacatca aatattcctt attaacccct
     8641 ttacaaatta aaaagctaaa ggtacacaat ttttgagcat agttattaat agcagacact
     8701 ctatgcctgt gtggagtaag aaaaaacagt atgttatgat tataactgtt atgcctactt
     8761 ataaaggtta cagaatattt ttccataatt ttcttgtata gcagtgcagc tttttccttt
     8821 gtggtgtaaa tagcaaagca agcaagagtt ctattactaa acacagcatg actcaaaaaa
     8881 cttagcaatt ctgaaggaaa gtccttgggg tcttctacct ttctcttctt ttttggagga
     8941 gtagaatgtt gagagtcagc agtagcctca tcatcactag atggcatttc ttctgagcaa
     9001 aacaggtttt cctcattaaa ggcattccac cactgctccc attcatcagt tccataggtt
     9061 ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac acttaaaaat
     9121 tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta tgtcacacca
     9181 cagaagtaag gttccttcac aaagatccgg ggcccactca taaatccagt tgccgccacg
     9241 gtagccaatc accgtatcgt ataaatcatc gtcggtacgt tcggcatcgc tcatcacaat
     9301 acgtgcctgg acgtcgagga tttcgcgtgg gtcaatgccg cgccagatcc acatcagacg
     9361 gttaatcatg cgataccagt gagggatggt tttaccatca agggccgact gcacaggcgg
     9421 ttgtgcgccg tgattaaagc ggcggactag cgtcgaggtt tcaggatgtt taaagcgggg
     9481 tttgaacagg gtttcgctca ggtttgcctg tgtcatggat gcagcctcca gaatacttac
     9541 tggaaactat tgtaacccgc ctgaagttaa aaagaacaac gcccggcagt gccaggcgtt
     9601 gaaaagatta gcgaccggag attggcggga cgaatacgac gcccatatcc cacggctgtt
     9661 caatccaggt atcttgcggg atatcaacaa catagtcatc aaccagcgga cgaccagccg
     9721 gttttgcgaa gatggtgaca aagtgcgctt ttggatacat ttcacgaatc gcaaccgcag
     9781 taccaccggt atccaccagg tcatcaataa cgatgaagcc ttcgccatcg ccttctgcgc
     9841 gtttcagcac tttaagctcg cgctggttgt cgtgatcgta gctggaaata caaacggtat
     9901 cgacatgacg aatacccagt tcacgcgcca gtaacgcacc cggtacagac cgccacggct
     9961 tacggcaata atgcctttcc attgttcaga aggcatcagt cggcttgcga gtttacgtgc
    10021 atggatctgc aacatgtccc aggtgacgat gtatttttcg ctcatgtgaa gtgtcccagc
    10081 ctgtttatct acggcttaaa aagtgttcga ggggaaaata ggttgcgcga gattatagag
    10141 atctggcgca ctaaaaacca gtatttcaca tgagtccgcg tctttttacg cactgcctct
    10201 ccctgacgcg ggataaagtg gtattctcaa acatatctcg caagcctgtc ttgtgtccaa
    10261 gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag
    10321 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tg
//
