LOCUS       sequence-405261-        9955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This vector encodes of a synCAM (MUC4) and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Stevens AJ, Harris AR, Gerdts J, Kim KH, Trentesaux C, Ramirez JT, 
            McKeithan WL, Fattahi F, Klein OD, Fletcher DA, Lim WA
  TITLE     Programming multicellular assembly with synthetic cell adhesion 
            molecules.
  JOURNAL   Nature. 2023 Feb;614(7946):144-152. doi: 10.1038/s41586-022-05622-z.
            Epub 2022 Dec 12.
  PUBMED    36509107
REFERENCE   2  (bases 1 to 9955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-05622-z"; journalName: "Nature"; date: 
            "2023-02"; volume: "614"; issue: "7946"; pages: "144-152"
COMMENT     Sequence Label: pHR-flag-GFP-MUC4
FEATURES             Location/Qualifiers
     source          1..9955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(151..480)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      165..300
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     465..485
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(555..572)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(726..1314)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(806..825)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1485..2276,2277..2345))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2108..2127
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2346..2450)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2518..2536
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2574..2596)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2696..2715
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2929..2948
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3238..3256
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3238..3255
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             3281..3514
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     3604..3620
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3605..3621
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3624..4212
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3730..3741
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4139..4159
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(4466..5176,5177..5179,5180..5182))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(5189..5212)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(5304..5711)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    5864..5981
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6266..6310)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6495..6728
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7225..7350
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7397..8030
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8198..8332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8222..8241)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8276..8295
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8757..8777)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8907..8972
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 aaaataggtt gcgcgagatt atagagatct ggcgcactaa aaaccagtat ttcacatgag
       61 tccgcgtctt tttacgcact gcctctccct gacgcgggat aaagtggtat tctcaaacat
      121 atctcgcaag cctgtcttgt gtccaagctt tttgcaaaag cctaggcctc caaaaaagcc
      181 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
      241 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
      301 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
      361 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
      421 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
      481 attccacagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      541 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      601 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      661 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      721 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      781 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      841 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      901 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      961 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1021 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1081 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1141 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1201 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1261 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1321 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1381 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1441 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1501 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1561 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1621 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1681 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1741 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1801 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1861 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1921 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     1981 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2041 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2101 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     2161 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2221 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     2281 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     2341 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2401 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2461 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2521 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     2581 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     2641 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     2701 tcagagcaga ttgtactgag agtgcaccat tcgacgctct cccttatgcg actcctgcat
     2761 taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc
     2821 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
     2881 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
     2941 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
     3001 gtagaggatc tggctagcga tgaccctgct gattggttcg ctgaccattt ccgggtgcgg
     3061 gacggcgtta ccagaaactc agaaggttcg tccaaccaaa ccgactctga cggcagttta
     3121 cgagagagat gatagggtct gcttcagtaa gccagatgct acacaattag gcttgtacat
     3181 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
     3241 taggtgacac tatagaatac acggaattaa ttctagatgc tgctagagat tttccacact
     3301 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact
     3361 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca
     3421 gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaagc aaaaagcaga
     3481 tcttgtcttc gttgggagtg aattagccct tccagtcccc ccttttcttt taaaaagtgg
     3541 ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag gtaccgagct cgaattaatt
     3601 ccctcgaggt cgacggtatc gatgcgggga ggcggcccaa agggagatcc gactcgtctg
     3661 agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag
     3721 gtccgctgga ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt
     3781 ggcaacacag gcgagcagcc aaggaaagga cgatgatttc cccgacaaca ccacggaatt
     3841 gtcagtgccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc
     3901 cgccgtggca atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc
     3961 aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct
     4021 gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa
     4081 aatgaaagcc atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca
     4141 tagcgtaaaa ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc
     4201 cagaggttga ttatcgataa gcttgatatc aagcttgcat gcctgcaggt cgactctaga
     4261 gtcgcggcct cagtcagtca cgcatgttgc aggtgggagt tgcggccgca agtcaaggat
     4321 cttatggcaa ggcttcggcg gaattaagga agtaggaaaa tcgcgcccct gagcaccccc
     4381 agaaccgaag tacaacgaat gttcccaccc caagcaacaa gagcccgccg agagctccaa
     4441 aaaaaatacc aaaaaaggcg gatcccttat acagctcatc cattcccagg gtgatcccag
     4501 cagccgtcac gaattcaagc agaaccatat ggtctctttt ttcgttagga tccttggaga
     4561 gggctgattg cgtggagagg tagtggttat ctggcagcaa aaccggaccg tcgccgatag
     4621 gcgtgttttg ctggtaatgg tcggccagtt gcacagagcc atcctcgata ttgtgtcgga
     4681 ttttgaagtt caccttgatg ccgttcttct gcttatcagc cataatatac acgttgtggc
     4741 tgttgtagtt gtactccagc ttgtggccca aaatattgcc gtcttctttg aaatcgattc
     4801 ctttcaactc aattctattt accaacgtgt ccccctcgaa cttaacttca gccctggttt
     4861 tatagttacc gtcgtccttg aagaatatgg tgcgttcctg aacatagccc tcaggcatcg
     4921 cggacttgaa gaaatcgtgc tgtttcatgt ggtcggggta ccgggagaag cactggactc
     4981 cgtaggtgag ggtggtcacg agtgtaggcc agggcactgg cagctttcct gttgtacaga
     5041 tgaacttcaa cgtcagttta ccgtacgtag cgtcgccctc tccttcgccg ctcactgaga
     5101 acttgtgtcc gttaacgtct ccgtccagct caaccaggat gggcacaact ccggtgaaca
     5161 attcttcacc cttagaaacc atggatcctt tatcatcgtc atctttataa tccgcaaaaa
     5221 caagacaaaa aatgtaactg agggcgataa ttgtcttcat ggtggcggcg acgcgtgaga
     5281 attctcgaga gctccagatc ccccccgggc gactcagtct gtcggaggac tggcgcgccg
     5341 agtgaggggt tgtgagctct tttatagagc tcgggaagca gaagcgcgcg aacagaagcg
     5401 agaagcaggc tgattggtta attcaaataa ggcgcagggt catttcaggt ccttggggga
     5461 gcctggaaac atctgatggg tcttaagaaa ctgctgaggg ttgggccata tctggggacc
     5521 atctgttctt ggcctcgggc cggggccgaa actgcggtga ccatctgttc ttggccccgg
     5581 gccggggccg aaactgctca ccgcagatat cctgtttggc ccaacgttag ctattttcat
     5641 gtacccgccc ttgatctgaa cttctctatt cttggtttgg tatttttcca tgccttgcaa
     5701 aatggcgtta ctgcagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
     5761 ttctggagac taaataaaat cttttatttt atcggggctg caggaattcg atatcaagct
     5821 tatcgatcca aactggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg
     5881 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
     5941 cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca
     6001 tttggcgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc
     6061 caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct
     6121 gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa
     6181 ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata taccacagcc
     6241 aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt
     6301 gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt
     6361 aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag
     6421 atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc
     6481 aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg
     6541 cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag
     6601 ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg
     6661 tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca
     6721 aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg
     6781 gtgctactcc taatggttca atttttacta ctttatattt atataattca cttctccaat
     6841 tgtccctcat atctcctcct ccaggtctga agatcagcgg ccggccgctt gctgtgcggt
     6901 ggtcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat
     6961 ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc
     7021 ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga
     7081 tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat
     7141 atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga
     7201 tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc
     7261 gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc
     7321 ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc
     7381 cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct gagggatctc
     7441 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
     7501 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
     7561 tctaaccaga gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc
     7621 tcgccactcc ccagtcccgc ccaggccacg cctccctgga aagtccccag cggaaagtcc
     7681 cttgtagcaa gctcgatatc agcagttctt gaagtactcc ggatgcagct ctcgggccac
     7741 gtgatgaaat gctaggcggc tgtcaaacct ccactctaac acttctctct ccgggtcatc
     7801 catcccatgc aggctcacag ggtgtaacaa gctggtgttc tctcctttat tggcctcttc
     7861 taccttatct ggctcaactg gtactagctt gtagcaccat ccaaaggtca gtggatatct
     7921 gacccctggc cctggtgtgt agttctgcta atcagggaag tagccttgtg tgtggtagat
     7981 ccacagatca aggatatctt gtcttctttg ggagtgaatt agcccttcca actactaagt
     8041 ttgtagtaca tatttaacaa atacaatttc tttaaaatga aaataattca gaggaatcac
     8101 aggtttagag taaatgaaac cacaggtaat tggcagtggt aatagggtat ggggtgggaa
     8161 gtttgggatg attttggtta gcttgagtta tccagttgat ccagacatga taagatacat
     8221 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     8281 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     8341 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     8401 gtaaaacctc tacaaatgtg gtatggctga ttatgatctc tagtcaaggc actatacatc
     8461 aaatattcct tattaacccc tttacaaatt aaaaagctaa aggtacacaa tttttgagca
     8521 tagttattaa tagcagacac tctatgcctg tgtggagtaa gaaaaaacag tatgttatga
     8581 ttataactgt tatgcctact tataaaggtt acagaatatt tttccataat tttcttgtat
     8641 agcagtgcag ctttttcctt tgtggtgtaa atagcaaagc aagcaagagt tctattacta
     8701 aacacagcat gactcaaaaa acttagcaat tctgaaggaa agtccttggg gtcttctacc
     8761 tttctcttct tttttggagg agtagaatgt tgagagtcag cagtagcctc atcatcacta
     8821 gatggcattt cttctgagca aaacaggttt tcctcattaa aggcattcca ccactgctcc
     8881 cattcatcag ttccataggt tggaatctaa aatacacaaa caattagaat cagtagttta
     8941 acacattata cacttaaaaa ttttatattt accttagagc tttaaatctc tgtaggtagt
     9001 ttgtccaatt atgtcacacc acagaagtaa ggttccttca caaagatccg gggcccactc
     9061 ataaatccag ttgccgccac ggtagccaat caccgtatcg tataaatcat cgtcggtacg
     9121 ttcggcatcg ctcatcacaa tacgtgcctg gacgtcgagg atttcgcgtg ggtcaatgcc
     9181 gcgccagatc cacatcagac ggttaatcat gcgataccag tgagggatgg ttttaccatc
     9241 aagggccgac tgcacaggcg gttgtgcgcc gtgattaaag cggcggacta gcgtcgaggt
     9301 ttcaggatgt ttaaagcggg gtttgaacag ggtttcgctc aggtttgcct gtgtcatgga
     9361 tgcagcctcc agaatactta ctggaaacta ttgtaacccg cctgaagtta aaaagaacaa
     9421 cgcccggcag tgccaggcgt tgaaaagatt agcgaccgga gattggcggg acgaatacga
     9481 cgcccatatc ccacggctgt tcaatccagg tatcttgcgg gatatcaaca acatagtcat
     9541 caaccagcgg acgaccagcc ggttttgcga agatggtgac aaagtgcgct tttggataca
     9601 tttcacgaat cgcaaccgca gtaccaccgg tatccaccag gtcatcaata acgatgaagc
     9661 cttcgccatc gccttctgcg cgtttcagca ctttaagctc gcgctggttg tcgtgatcgt
     9721 agctggaaat acaaacggta tcgacatgac gaatacccag ttcacgcgcc agtaacgcac
     9781 ccggtacaga ccgccacggc ttacggcaat aatgcctttc cattgttcag aaggcatcag
     9841 tcggcttgcg agtttacgtg catggatctg caacatgtcc caggtgacga tgtatttttc
     9901 gctcatgtga agtgtcccag cctgtttatc tacggcttaa aaagtgttcg agggg
//
