LOCUS       sequence-405262-       10243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This vector encodes of a synCAM (NCAM1) and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Stevens AJ, Harris AR, Gerdts J, Kim KH, Trentesaux C, Ramirez JT, 
            McKeithan WL, Fattahi F, Klein OD, Fletcher DA, Lim WA
  TITLE     Programming multicellular assembly with synthetic cell adhesion 
            molecules.
  JOURNAL   Nature. 2023 Feb;614(7946):144-152. doi: 10.1038/s41586-022-05622-z.
            Epub 2022 Dec 12.
  PUBMED    36509107
REFERENCE   2  (bases 1 to 10243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-05622-z"; journalName: "Nature"; date: 
            "2023-02"; volume: "614"; issue: "7946"; pages: "144-152"
COMMENT     Sequence Label: pHR-flag-GFP-NCAM-1
FEATURES             Location/Qualifiers
     source          1..10243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     242..262
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(332..349)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(503..1091)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(583..602)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1262..2053,2054..2122))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1885..1904
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2123..2227)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2295..2313
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2351..2373)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2473..2492
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2706..2725
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3015..3033
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3015..3032
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             3058..3291
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     3381..3397
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3382..3398
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3401..3989
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3507..3518
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3916..3936
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(4531..5241,5242..5244,5245..5247))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(5254..5277)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(5369..5776)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    5929..6046
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6331..6375)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6560..6793
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7290..7415
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7462..8095
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8263..8397
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8287..8306)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8341..8360
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8822..8842)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8972..9037
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(join(10171..10243,1..257))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10185..10243,1..77)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc
       61 ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga
      121 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct
      181 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
      241 caccctaact gacacacatt ccacagctgc attaatgaat cggccaacgc gcggggagag
      301 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      361 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      421 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      481 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      541 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      601 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      661 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      721 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      781 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      841 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      901 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
      961 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1021 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1081 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1141 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1201 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1261 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1321 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1381 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1441 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1501 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1561 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1621 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1681 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1741 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1801 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1861 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     1921 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     1981 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2041 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2101 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2161 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2221 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     2281 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     2341 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     2401 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     2461 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccattcg acgctctccc
     2521 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc
     2581 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
     2641 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
     2701 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
     2761 gccacgatgc gtccggcgta gaggatctgg ctagcgatga ccctgctgat tggttcgctg
     2821 accatttccg ggtgcgggac ggcgttacca gaaactcaga aggttcgtcc aaccaaaccg
     2881 actctgacgg cagtttacga gagagatgat agggtctgct tcagtaagcc agatgctaca
     2941 caattaggct tgtacatatt gtcgttagaa cgcggctaca attaatacat aaccttatgt
     3001 atcatacaca tacgatttag gtgacactat agaatacacg gaattaattc tagatgctgc
     3061 tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca
     3121 acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg
     3181 ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag
     3241 tacaagcaaa aagcagatct tgtcttcgtt gggagtgaat tagcccttcc agtcccccct
     3301 tttcttttaa aaagtggcta agatctacag ctgccttgta agtcattggt cttaaaggta
     3361 ccgagctcga attaattccc tcgaggtcga cggtatcgat gcggggaggc ggcccaaagg
     3421 gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc
     3481 aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc
     3541 ccgcgcagaa tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc
     3601 gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg
     3661 caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg
     3721 agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac
     3781 accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat
     3841 ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt
     3901 aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt
     3961 tcacaaattt tgtaatccag aggttgatta tcgataagct tgatatcaag cttgcatgcc
     4021 tgcaggtcga ctctagagtc gcggcctcag tcagtcacgc atgttgcagg tgggagttgc
     4081 ggccgcaagt caaggatctt acgccttgct ctcgttctcc ttcgtttgcg tcgcgtcatt
     4141 gggaactgtt ttgacctccg ctggcgcagg cttagtctct gtctcctggc actcaggttt
     4201 agcttccaca ggcccctttt ccggttcggt cagcggtgtg gtctcgtttg gctccgtatg
     4261 tttgccccca tcgtgattag gtgttctttc ttcttcagtc cgaacctcta cgatgggctc
     4321 tttagattca tccttagaga aagccgcctt gccctcctcc atgtccttgc ctttagcccc
     4381 aggcccagcc ttcccgcaga gattgaccgc aatacacatg aacaagccgc atttattaag
     4441 gaaatagcat gttatatcca ccaccaccag caggagtaca aagataacta taagaatccc
     4501 gactatcgca cccgtactaa ggccggatcc cttatacagc tcatccattc ccagggtgat
     4561 cccagcagcc gtcacgaatt caagcagaac catatggtct cttttttcgt taggatcctt
     4621 ggagagggct gattgcgtgg agaggtagtg gttatctggc agcaaaaccg gaccgtcgcc
     4681 gataggcgtg ttttgctggt aatggtcggc cagttgcaca gagccatcct cgatattgtg
     4741 tcggattttg aagttcacct tgatgccgtt cttctgctta tcagccataa tatacacgtt
     4801 gtggctgttg tagttgtact ccagcttgtg gcccaaaata ttgccgtctt ctttgaaatc
     4861 gattcctttc aactcaattc tatttaccaa cgtgtccccc tcgaacttaa cttcagccct
     4921 ggttttatag ttaccgtcgt ccttgaagaa tatggtgcgt tcctgaacat agccctcagg
     4981 catcgcggac ttgaagaaat cgtgctgttt catgtggtcg gggtaccggg agaagcactg
     5041 gactccgtag gtgagggtgg tcacgagtgt aggccagggc actggcagct ttcctgttgt
     5101 acagatgaac ttcaacgtca gtttaccgta cgtagcgtcg ccctctcctt cgccgctcac
     5161 tgagaacttg tgtccgttaa cgtctccgtc cagctcaacc aggatgggca caactccggt
     5221 gaacaattct tcacccttag aaaccatgga tcctttatca tcgtcatctt tataatccgc
     5281 aaaaacaaga caaaaaatgt aactgagggc gataattgtc ttcatggtgg cggcgacgcg
     5341 tgagaattct cgagagctcc agatcccccc cgggcgactc agtctgtcgg aggactggcg
     5401 cgccgagtga ggggttgtga gctcttttat agagctcggg aagcagaagc gcgcgaacag
     5461 aagcgagaag caggctgatt ggttaattca aataaggcgc agggtcattt caggtccttg
     5521 ggggagcctg gaaacatctg atgggtctta agaaactgct gagggttggg ccatatctgg
     5581 ggaccatctg ttcttggcct cgggccgggg ccgaaactgc ggtgaccatc tgttcttggc
     5641 cccgggccgg ggccgaaact gctcaccgca gatatcctgt ttggcccaac gttagctatt
     5701 ttcatgtacc cgcccttgat ctgaacttct ctattcttgg tttggtattt ttccatgcct
     5761 tgcaaaatgg cgttactgca gctagcttgc caaacctaca ggtggggtct ttcattcccc
     5821 cctttttctg gagactaaat aaaatctttt attttatcgg ggctgcagga attcgatatc
     5881 aagcttatcg atccaaactg gatctctgct gtccctgtaa taaacccgaa aattttgaat
     5941 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
     6001 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac
     6061 tgccatttgg cgataccgtc gagatccgtt cactaatcga atggatctgt ctctgtctct
     6121 ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg
     6181 ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc
     6241 aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca
     6301 cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta attccaataa
     6361 ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct
     6421 tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc
     6481 cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc
     6541 agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat
     6601 tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc
     6661 aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg
     6721 taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag
     6781 gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt
     6841 ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata attcacttct
     6901 ccaattgtcc ctcatatctc ctcctccagg tctgaagatc agcggccggc cgcttgctgt
     6961 gcggtggtct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct
     7021 tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta
     7081 agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct
     7141 tctgatgttt ctaacaggcc aggattaact gcgaatcgtt ctagctccct gcttgcccat
     7201 actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat tttttcccat
     7261 cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct ctccttctag
     7321 cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg
     7381 cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc tttcgctttc
     7441 aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     7501 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     7561 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     7621 tctggtctaa ccagagagac ccagtacagg caaaaagcag ctgcttatat gcaggatctg
     7681 agggctcgcc actccccagt cccgcccagg ccacgcctcc ctggaaagtc cccagcggaa
     7741 agtcccttgt agcaagctcg atatcagcag ttcttgaagt actccggatg cagctctcgg
     7801 gccacgtgat gaaatgctag gcggctgtca aacctccact ctaacacttc tctctccggg
     7861 tcatccatcc catgcaggct cacagggtgt aacaagctgg tgttctctcc tttattggcc
     7921 tcttctacct tatctggctc aactggtact agcttgtagc accatccaaa ggtcagtgga
     7981 tatctgaccc ctggccctgg tgtgtagttc tgctaatcag ggaagtagcc ttgtgtgtgg
     8041 tagatccaca gatcaaggat atcttgtctt ctttgggagt gaattagccc ttccaactac
     8101 taagtttgta gtacatattt aacaaataca atttctttaa aatgaaaata attcagagga
     8161 atcacaggtt tagagtaaat gaaaccacag gtaattggca gtggtaatag ggtatggggt
     8221 gggaagtttg ggatgatttt ggttagcttg agttatccag ttgatccaga catgataaga
     8281 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     8341 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     8401 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     8461 agcaagtaaa acctctacaa atgtggtatg gctgattatg atctctagtc aaggcactat
     8521 acatcaaata ttccttatta acccctttac aaattaaaaa gctaaaggta cacaattttt
     8581 gagcatagtt attaatagca gacactctat gcctgtgtgg agtaagaaaa aacagtatgt
     8641 tatgattata actgttatgc ctacttataa aggttacaga atatttttcc ataattttct
     8701 tgtatagcag tgcagctttt tcctttgtgg tgtaaatagc aaagcaagca agagttctat
     8761 tactaaacac agcatgactc aaaaaactta gcaattctga aggaaagtcc ttggggtctt
     8821 ctacctttct cttctttttt ggaggagtag aatgttgaga gtcagcagta gcctcatcat
     8881 cactagatgg catttcttct gagcaaaaca ggttttcctc attaaaggca ttccaccact
     8941 gctcccattc atcagttcca taggttggaa tctaaaatac acaaacaatt agaatcagta
     9001 gtttaacaca ttatacactt aaaaatttta tatttacctt agagctttaa atctctgtag
     9061 gtagtttgtc caattatgtc acaccacaga agtaaggttc cttcacaaag atccggggcc
     9121 cactcataaa tccagttgcc gccacggtag ccaatcaccg tatcgtataa atcatcgtcg
     9181 gtacgttcgg catcgctcat cacaatacgt gcctggacgt cgaggatttc gcgtgggtca
     9241 atgccgcgcc agatccacat cagacggtta atcatgcgat accagtgagg gatggtttta
     9301 ccatcaaggg ccgactgcac aggcggttgt gcgccgtgat taaagcggcg gactagcgtc
     9361 gaggtttcag gatgtttaaa gcggggtttg aacagggttt cgctcaggtt tgcctgtgtc
     9421 atggatgcag cctccagaat acttactgga aactattgta acccgcctga agttaaaaag
     9481 aacaacgccc ggcagtgcca ggcgttgaaa agattagcga ccggagattg gcgggacgaa
     9541 tacgacgccc atatcccacg gctgttcaat ccaggtatct tgcgggatat caacaacata
     9601 gtcatcaacc agcggacgac cagccggttt tgcgaagatg gtgacaaagt gcgcttttgg
     9661 atacatttca cgaatcgcaa ccgcagtacc accggtatcc accaggtcat caataacgat
     9721 gaagccttcg ccatcgcctt ctgcgcgttt cagcacttta agctcgcgct ggttgtcgtg
     9781 atcgtagctg gaaatacaaa cggtatcgac atgacgaata cccagttcac gcgccagtaa
     9841 cgcacccggt acagaccgcc acggcttacg gcaataatgc ctttccattg ttcagaaggc
     9901 atcagtcggc ttgcgagttt acgtgcatgg atctgcaaca tgtcccaggt gacgatgtat
     9961 ttttcgctca tgtgaagtgt cccagcctgt ttatctacgg cttaaaaagt gttcgagggg
    10021 aaaataggtt gcgcgagatt atagagatct ggcgcactaa aaaccagtat ttcacatgag
    10081 tccgcgtctt tttacgcact gcctctccct gacgcgggat aaagtggtat tctcaaacat
    10141 atctcgcaag cctgtcttgt gtccaagctt tttgcaaaag cctaggcctc caaaaaagcc
    10201 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcg
//
