LOCUS       sequence-405263-       10000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This vector encodes of a synCAM (JAM-B) and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Stevens AJ, Harris AR, Gerdts J, Kim KH, Trentesaux C, Ramirez JT, 
            McKeithan WL, Fattahi F, Klein OD, Fletcher DA, Lim WA
  TITLE     Programming multicellular assembly with synthetic cell adhesion 
            molecules.
  JOURNAL   Nature. 2023 Feb;614(7946):144-152. doi: 10.1038/s41586-022-05622-z.
            Epub 2022 Dec 12.
  PUBMED    36509107
REFERENCE   2  (bases 1 to 10000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-05622-z"; journalName: "Nature"; date: 
            "2023-02"; volume: "614"; issue: "7946"; pages: "144-152"
COMMENT     Sequence Label: pHR-flag-GFP-JAM-B
FEATURES             Location/Qualifiers
     source          1..10000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     120..137
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(207..227)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        212..541
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      392..527
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1675..1740
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1870..1890
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2315..2449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2352..2371)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2406..2425
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2617..3250
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3297..3422
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3919..4152
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4337..4381
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4666..4783
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4936..5343
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             5435..5458
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(5465..5467,5468..5470,5471..6181)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    6480..7068
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6533..6553)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6951..6962)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7071..7087)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7072..7088)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7178..7411
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7436..7454)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7437..7454)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7744..7763)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7977..7996)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8096..8118
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8156..8174)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8242..8346
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8347..8415,8416..9207)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8565..8584)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9378..9966
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9867..9886
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
       61 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
      121 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
      181 gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
      241 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
      301 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
      361 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
      421 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
      481 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
      541 aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt
      601 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
      661 cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata
      721 aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga
      781 tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg
      841 ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca
      901 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
      961 gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
     1021 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
     1081 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
     1141 gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
     1201 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
     1261 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
     1321 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
     1381 cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga
     1441 ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg
     1501 cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg
     1561 gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac
     1621 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     1681 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     1741 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     1801 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     1861 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     1921 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     1981 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
     2041 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
     2101 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
     2161 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
     2221 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     2281 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     2341 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2401 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
     2461 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
     2521 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
     2581 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
     2641 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     2701 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     2761 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     2821 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     2881 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     2941 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     3001 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     3061 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     3121 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     3181 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     3241 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     3301 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     3361 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     3421 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     3481 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     3541 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     3601 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     3661 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     3721 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
     3781 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     3841 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     3901 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     3961 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     4021 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     4081 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     4141 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     4201 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     4261 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     4321 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     4381 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     4441 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     4501 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     4561 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     4621 gaacggatct cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg
     4681 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     4741 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     4801 cagcagagat ccagtttgga tcgataagct tgatatcgaa ttcctgcagc cccgataaaa
     4861 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
     4921 ggcaagctag ctgcagtaac gccattttgc aaggcatgga aaaataccaa accaagaata
     4981 gagaagttca gatcaagggc gggtacatga aaatagctaa cgttgggcca aacaggatat
     5041 ctgcggtgag cagtttcggc cccggcccgg ggccaagaac agatggtcac cgcagtttcg
     5101 gccccggccc gaggccaaga acagatggtc cccagatatg gcccaaccct cagcagtttc
     5161 ttaagaccca tcagatgttt ccaggctccc ccaaggacct gaaatgaccc tgcgccttat
     5221 ttgaattaac caatcagcct gcttctcgct tctgttcgcg cgcttctgct tcccgagctc
     5281 tataaaagag ctcacaaccc ctcactcggc gcgccagtcc tccgacagac tgagtcgccc
     5341 gggggggatc tggagctctc gagaattctc acgcgtcgcc gccaccatga agacaattat
     5401 cgccctcagt tacatttttt gtcttgtttt tgcggattat aaagatgacg atgataaagg
     5461 atccatggtt tctaagggtg aagaattgtt caccggagtt gtgcccatcc tggttgagct
     5521 ggacggagac gttaacggac acaagttctc agtgagcggc gaaggagagg gcgacgctac
     5581 gtacggtaaa ctgacgttga agttcatctg tacaacagga aagctgccag tgccctggcc
     5641 tacactcgtg accaccctca cctacggagt ccagtgcttc tcccggtacc ccgaccacat
     5701 gaaacagcac gatttcttca agtccgcgat gcctgagggc tatgttcagg aacgcaccat
     5761 attcttcaag gacgacggta actataaaac cagggctgaa gttaagttcg agggggacac
     5821 gttggtaaat agaattgagt tgaaaggaat cgatttcaaa gaagacggca atattttggg
     5881 ccacaagctg gagtacaact acaacagcca caacgtgtat attatggctg ataagcagaa
     5941 gaacggcatc aaggtgaact tcaaaatccg acacaatatc gaggatggct ctgtgcaact
     6001 ggccgaccat taccagcaaa acacgcctat cggcgacggt ccggttttgc tgccagataa
     6061 ccactacctc tccacgcaat cagccctctc caaggatcct aacgaaaaaa gagaccatat
     6121 ggttctgctt gaattcgtga cggctgctgg gatcaccctg ggaatggatg agctgtataa
     6181 gggatccggg atcatcgcgg cagttgtcgt agtggcgctc gtgatttctg tgtgcggtct
     6241 tggagtttgc tacgcacagc ggaaaggtta tttctctaaa gagacttcat tccagaagag
     6301 taattctagc agtaaagcta caaccatgag tgagaacgat ttcaagcata ccaagtcatt
     6361 cattatataa gatccttgac ttgcggccgc aactcccacc tgcaacatgc gtgactgact
     6421 gaggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata
     6481 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     6541 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6601 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6661 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6721 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6781 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6841 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     6901 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6961 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     7021 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     7081 gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta caaggcagct
     7141 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     7201 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     7261 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7321 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7381 cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat
     7441 agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt
     7501 ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat
     7561 catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg
     7621 gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc
     7681 agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     7741 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     7801 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     7861 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     7921 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca
     7981 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     8041 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     8101 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     8161 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     8221 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     8281 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     8341 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     8401 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     8461 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     8521 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     8581 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     8641 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     8701 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     8761 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     8821 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     8881 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     8941 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     9001 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     9061 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     9121 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     9181 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     9241 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     9301 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     9361 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     9421 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     9481 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     9541 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     9601 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     9661 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     9721 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     9781 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9841 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9901 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9961 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
//
