LOCUS       sequence-405264-       10372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This vector encodes of a synCAM (DLL1) and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Stevens AJ, Harris AR, Gerdts J, Kim KH, Trentesaux C, Ramirez JT, 
            McKeithan WL, Fattahi F, Klein OD, Fletcher DA, Lim WA
  TITLE     Programming multicellular assembly with synthetic cell adhesion 
            molecules.
  JOURNAL   Nature. 2023 Feb;614(7946):144-152. doi: 10.1038/s41586-022-05622-z.
            Epub 2022 Dec 12.
  PUBMED    36509107
REFERENCE   2  (bases 1 to 10372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-05622-z"; journalName: "Nature"; date: 
            "2023-02"; volume: "614"; issue: "7946"; pages: "144-152"
COMMENT     Sequence Label: pHR-flag-GFP-DLL1
FEATURES             Location/Qualifiers
     source          1..10372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..42)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(196..784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(276..295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(955..1746,1747..1815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1578..1597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1816..1920)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1988..2006
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2044..2066)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2166..2185
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2399..2418
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2708..2726
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2708..2725
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             2751..2984
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     3074..3090
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3075..3091
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3094..3682
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3200..3211
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3609..3629
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(4353..5063,5064..5066,5067..5069))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(5076..5099)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(5191..5598)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    5751..5868
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6153..6197)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6382..6615
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7112..7237
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7284..7917
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8085..8219
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8109..8128)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8163..8182
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8644..8664)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8794..8859
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(9993..10322)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10007..10142
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10307..10327
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
       61 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      841 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      901 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
      961 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1021 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1081 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1141 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1201 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1261 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1321 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1381 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1441 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1501 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     1561 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     1621 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     1681 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     1741 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     1801 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     1861 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     1921 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     1981 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     2041 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     2101 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     2161 ctatgcggca tcagagcaga ttgtactgag agtgcaccat tcgacgctct cccttatgcg
     2221 actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa
     2281 ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
     2341 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
     2401 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
     2461 tgcgtccggc gtagaggatc tggctagcga tgaccctgct gattggttcg ctgaccattt
     2521 ccgggtgcgg gacggcgtta ccagaaactc agaaggttcg tccaaccaaa ccgactctga
     2581 cggcagttta cgagagagat gatagggtct gcttcagtaa gccagatgct acacaattag
     2641 gcttgtacat attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac
     2701 acatacgatt taggtgacac tatagaatac acggaattaa ttctagatgc tgctagagat
     2761 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
     2821 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
     2881 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaagc
     2941 aaaaagcaga tcttgtcttc gttgggagtg aattagccct tccagtcccc ccttttcttt
     3001 taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag gtaccgagct
     3061 cgaattaatt ccctcgaggt cgacggtatc gatgcgggga ggcggcccaa agggagatcc
     3121 gactcgtctg agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc
     3181 gggaaggaag gtccgctgga ttgagggccg aagggacgta gcagaaggac gtcccgcgca
     3241 gaatccaggt ggcaacacag gcgagcagcc aaggaaagga cgatgatttc cccgacaaca
     3301 ccacggaatt gtcagtgccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg
     3361 cgatgagttc cgccgtggca atagggaggg ggaaagcgaa agtcccggaa aggagctgac
     3421 aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc
     3481 cacgttgcct gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca
     3541 aggaggagaa aatgaaagcc atacgggaag caatagcatg atacaaaggc attaaagcag
     3601 cgtatccaca tagcgtaaaa ggagcaacat agttaagaat accagtcaat ctttcacaaa
     3661 ttttgtaatc cagaggttga ttatcgataa gcttgatatc aagcttgcat gcctgcaggt
     3721 cgactctaga gtcgcggcct cagtcagtca cgcatgttgc aggtgggagt tgcggccgca
     3781 agtcaaggat cttacacttc agtagctatt acgcattcgt ctttctcctc tgaaataaca
     3841 tacacggatt ggtatttagt gtctttggaa gtagagcacc ctgagtcagg tcgcttcctc
     3901 tcgcttgctt cgcctcccct gagcgtggtt ggcgttcctt tttcctcacc agatgagccc
     3961 tggggttgac acttggtatc tcgctttgag tgcgcatctc gcacggctgt gtcgtcacct
     4021 ttcagatcct ggaccagatt atagtccacg gccggatatc tggccttgaa accatttttg
     4081 tcggcggaat gatcaccatg aaaatcggcc ttcttgttag tgtttttaat ttgcgtagcc
     4141 cctattattg agacacttat atctttttcc ctttggcaat tagccagatt gttcatggtc
     4201 tctgtttcgc ctcggcatgg atctgccggg ggccgatgct tctgcaatct caggcgtaca
     4261 cagacgacga cagcggcgca gcccagaagc aacatgagga cgagtataac acctgcgcat
     4321 actgctaccc agggaaacgg cccaccggat cccttataca gctcatccat tcccagggtg
     4381 atcccagcag ccgtcacgaa ttcaagcaga accatatggt ctcttttttc gttaggatcc
     4441 ttggagaggg ctgattgcgt ggagaggtag tggttatctg gcagcaaaac cggaccgtcg
     4501 ccgataggcg tgttttgctg gtaatggtcg gccagttgca cagagccatc ctcgatattg
     4561 tgtcggattt tgaagttcac cttgatgccg ttcttctgct tatcagccat aatatacacg
     4621 ttgtggctgt tgtagttgta ctccagcttg tggcccaaaa tattgccgtc ttctttgaaa
     4681 tcgattcctt tcaactcaat tctatttacc aacgtgtccc cctcgaactt aacttcagcc
     4741 ctggttttat agttaccgtc gtccttgaag aatatggtgc gttcctgaac atagccctca
     4801 ggcatcgcgg acttgaagaa atcgtgctgt ttcatgtggt cggggtaccg ggagaagcac
     4861 tggactccgt aggtgagggt ggtcacgagt gtaggccagg gcactggcag ctttcctgtt
     4921 gtacagatga acttcaacgt cagtttaccg tacgtagcgt cgccctctcc ttcgccgctc
     4981 actgagaact tgtgtccgtt aacgtctccg tccagctcaa ccaggatggg cacaactccg
     5041 gtgaacaatt cttcaccctt agaaaccatg gatcctttat catcgtcatc tttataatcc
     5101 gcaaaaacaa gacaaaaaat gtaactgagg gcgataattg tcttcatggt ggcggcgacg
     5161 cgtgagaatt ctcgagagct ccagatcccc cccgggcgac tcagtctgtc ggaggactgg
     5221 cgcgccgagt gaggggttgt gagctctttt atagagctcg ggaagcagaa gcgcgcgaac
     5281 agaagcgaga agcaggctga ttggttaatt caaataaggc gcagggtcat ttcaggtcct
     5341 tgggggagcc tggaaacatc tgatgggtct taagaaactg ctgagggttg ggccatatct
     5401 ggggaccatc tgttcttggc ctcgggccgg ggccgaaact gcggtgacca tctgttcttg
     5461 gccccgggcc ggggccgaaa ctgctcaccg cagatatcct gtttggccca acgttagcta
     5521 ttttcatgta cccgcccttg atctgaactt ctctattctt ggtttggtat ttttccatgc
     5581 cttgcaaaat ggcgttactg cagctagctt gccaaaccta caggtggggt ctttcattcc
     5641 cccctttttc tggagactaa ataaaatctt ttattttatc ggggctgcag gaattcgata
     5701 tcaagcttat cgatccaaac tggatctctg ctgtccctgt aataaacccg aaaattttga
     5761 atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact
     5821 attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat
     5881 actgccattt ggcgataccg tcgagatccg ttcactaatc gaatggatct gtctctgtct
     5941 ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg
     6001 tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca
     6061 gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac
     6121 cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat
     6181 aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag
     6241 cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt
     6301 tccagagatt tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt
     6361 ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg
     6421 attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc
     6481 tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc
     6541 tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca
     6601 aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc
     6661 ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata taattcactt
     6721 ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg gccgcttgct
     6781 gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt
     6841 cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc
     6901 taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc
     6961 cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc
     7021 atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc
     7081 atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct
     7141 agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg
     7201 tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt
     7261 tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag
     7321 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     7381 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     7441 gatctggtct aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc
     7501 tgagggctcg ccactcccca gtcccgccca ggccacgcct ccctggaaag tccccagcgg
     7561 aaagtccctt gtagcaagct cgatatcagc agttcttgaa gtactccgga tgcagctctc
     7621 gggccacgtg atgaaatgct aggcggctgt caaacctcca ctctaacact tctctctccg
     7681 ggtcatccat cccatgcagg ctcacagggt gtaacaagct ggtgttctct cctttattgg
     7741 cctcttctac cttatctggc tcaactggta ctagcttgta gcaccatcca aaggtcagtg
     7801 gatatctgac ccctggccct ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt
     7861 ggtagatcca cagatcaagg atatcttgtc ttctttggga gtgaattagc ccttccaact
     7921 actaagtttg tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag
     7981 gaatcacagg tttagagtaa atgaaaccac aggtaattgg cagtggtaat agggtatggg
     8041 gtgggaagtt tgggatgatt ttggttagct tgagttatcc agttgatcca gacatgataa
     8101 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     8161 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     8221 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     8281 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctctag tcaaggcact
     8341 atacatcaaa tattccttat taaccccttt acaaattaaa aagctaaagg tacacaattt
     8401 ttgagcatag ttattaatag cagacactct atgcctgtgt ggagtaagaa aaaacagtat
     8461 gttatgatta taactgttat gcctacttat aaaggttaca gaatattttt ccataatttt
     8521 cttgtatagc agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag caagagttct
     8581 attactaaac acagcatgac tcaaaaaact tagcaattct gaaggaaagt ccttggggtc
     8641 ttctaccttt ctcttctttt ttggaggagt agaatgttga gagtcagcag tagcctcatc
     8701 atcactagat ggcatttctt ctgagcaaaa caggttttcc tcattaaagg cattccacca
     8761 ctgctcccat tcatcagttc cataggttgg aatctaaaat acacaaacaa ttagaatcag
     8821 tagtttaaca cattatacac ttaaaaattt tatatttacc ttagagcttt aaatctctgt
     8881 aggtagtttg tccaattatg tcacaccaca gaagtaaggt tccttcacaa agatccgggg
     8941 cccactcata aatccagttg ccgccacggt agccaatcac cgtatcgtat aaatcatcgt
     9001 cggtacgttc ggcatcgctc atcacaatac gtgcctggac gtcgaggatt tcgcgtgggt
     9061 caatgccgcg ccagatccac atcagacggt taatcatgcg ataccagtga gggatggttt
     9121 taccatcaag ggccgactgc acaggcggtt gtgcgccgtg attaaagcgg cggactagcg
     9181 tcgaggtttc aggatgttta aagcggggtt tgaacagggt ttcgctcagg tttgcctgtg
     9241 tcatggatgc agcctccaga atacttactg gaaactattg taacccgcct gaagttaaaa
     9301 agaacaacgc ccggcagtgc caggcgttga aaagattagc gaccggagat tggcgggacg
     9361 aatacgacgc ccatatccca cggctgttca atccaggtat cttgcgggat atcaacaaca
     9421 tagtcatcaa ccagcggacg accagccggt tttgcgaaga tggtgacaaa gtgcgctttt
     9481 ggatacattt cacgaatcgc aaccgcagta ccaccggtat ccaccaggtc atcaataacg
     9541 atgaagcctt cgccatcgcc ttctgcgcgt ttcagcactt taagctcgcg ctggttgtcg
     9601 tgatcgtagc tggaaataca aacggtatcg acatgacgaa tacccagttc acgcgccagt
     9661 aacgcacccg gtacagaccg ccacggctta cggcaataat gcctttccat tgttcagaag
     9721 gcatcagtcg gcttgcgagt ttacgtgcat ggatctgcaa catgtcccag gtgacgatgt
     9781 atttttcgct catgtgaagt gtcccagcct gtttatctac ggcttaaaaa gtgttcgagg
     9841 ggaaaatagg ttgcgcgaga ttatagagat ctggcgcact aaaaaccagt atttcacatg
     9901 agtccgcgtc tttttacgca ctgcctctcc ctgacgcggg ataaagtggt attctcaaac
     9961 atatctcgca agcctgtctt gtgtccaagc tttttgcaaa agcctaggcc tccaaaaaag
    10021 cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata
    10081 aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg
    10141 atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata
    10201 cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc
    10261 atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac
    10321 acattccaca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt tt
//
