LOCUS       sequence-405272-        1503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the Sma ES4 insert with a chloramphenicol
            resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo37
FEATURES             Location/Qualifiers
     source          1..1503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      386..413
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(434..1093)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGE"
     primer_bind     1008..1027
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1094..1196)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      join(1255..1503,1..140)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 aaaaaaaaga atatataagg cttttaaagc ttttaaggtt taacggttgt ggacaacaag
       61 ccagggatgt aacgcactga gaagccctta gagcctctca aagcaatttt gagtgacaca
      121 ggaacactta acggctgaca tgggggtctc ggagacacag tggcctgctg gccccgcagc
      181 gccggttcaa cctctgacag ggggagggcg gttcagcccc ggtccttgtt cccggagccc
      241 ctgaccgtta caatttcgcg gccgttttcc ggcaacctgc ctcgcgcagg gttcatcctc
      301 gtagtccacg gcgagcctgg cggtgcgcga agcgcgttcc tgccggccac gcatcacaga
      361 gaaaaagatt acgagacctc acactggctc accttcgggt gggcctttct gcgtttatac
      421 ctaggagtca aaattactcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
      481 gcattctacc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
      541 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
      601 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
      661 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
      721 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
      781 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
      841 atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca ttcatcaggc
      901 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
      961 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     1021 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     1081 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     1141 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcagtca
     1201 attcgagggg gatcaattcc gtattgatcc ggccacgatg cgtccggcgt agaggatctg
     1261 aagatcagca gttcaacctg ttgatagtac gtactaagct ctcatgtttc acgtactaag
     1321 ctctcatgtt taacgtacta agctctcatg tttaacgaac taaaccctca tggctaacgt
     1381 actaagctct catggctaac gtactaagct ctcatgtttc acgtactaag ctctcatgtt
     1441 tgaacaataa aattaatata aatcagcaac ttaaatagcc tctaaggttt taagttttat
     1501 aag
//
