LOCUS       sequence-405274-        1686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the Sma ES5 insert with a chloramphenicol
            resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo38
FEATURES             Location/Qualifiers
     source          1..1686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..95
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(116..775)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGE"
     primer_bind     690..709
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(776..878)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      937..1325
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 caccgccaaa ggagttgtag agaatgaaca agaccgaatt gatcgatgca ttacgagacc
       61 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat acctaggagt caaaattact
      121 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattcta ccgacatgga
      181 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
      241 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
      301 ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa
      361 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
      421 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
      481 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
      541 ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
      601 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
      661 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
      721 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
      781 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
      841 cattatggtg aaagttggaa cctcttacgt gccgatcagt caattcgagg gggatcaatt
      901 ccgtattgat ccggccacga tgcgtccggc gtagaggatc tgaagatcag cagttcaacc
      961 tgttgatagt acgtactaag ctctcatgtt tcacgtacta agctctcatg tttaacgtac
     1021 taagctctca tgtttaacga actaaaccct catggctaac gtactaagct ctcatggcta
     1081 acgtactaag ctctcatgtt tcacgtacta agctctcatg tttgaacaat aaaattaata
     1141 taaatcagca acttaaatag cctctaaggt tttaagtttt ataagaaaaa aaagaatata
     1201 taaggctttt aaagctttta aggtttaacg gttgtggaca acaagccagg gatgtaacgc
     1261 actgagaagc ccttagagcc tctcaaagca attttgagtg acacaggaac acttaacggc
     1321 tgacatgggg gtctcggaga ctgcagatcg tgccggtgaa gtacatcgag gaagtgctgg
     1381 acctggcgct ggagcgtccg ttggcaccga agaaggcgcg caagagtgcg cagcgcgtca
     1441 cggtgcgcag caaggccaaa ccgagtggaa acgcgcgcgt caagcattga cgcgcgctgt
     1501 cgaaatgcct gaaacccgcg tcgttgctgg ctttccacct tgcgtggggg taggggcgct
     1561 ggtataaaag cagcacccgc gaatgagtga tcgcagtctg gtgatcactg aattcggcga
     1621 acagtttcca catggcggcc gtgcgcgcat cgcgtgcggt tgcttccctg tcgaataagc
     1681 ggaacc
//
