LOCUS       sequence-405275-        1644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the Bpi ES8 insert with a chloramphenicol
            resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo33
FEATURES             Location/Qualifiers
     source          1..1644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      429..456
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(477..1136)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGE"
     primer_bind     1051..1070
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1137..1239)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      join(1298..1644,1..42)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 caaagcaatt ttgagtgaca caggaacact taacggctga catgggggtc tcggagacgt
       61 tacggctaaa tctgctgcac caaagaccgg cgcttttacc agctaatccc atacaataga
      121 gcatgaattt tgataggcgg cgggcggaag cttgccgcct attttgcgtg tcttcgcctc
      181 tctcagccct cttcctgagc gatccgcttt tcagcagctc tgtcgggtaa gttgtgcgga
      241 tggctatcca cacctcccaa ataggtagac aattactctt gtaattttaa ttatacatct
      301 gtattcagaa tgagagttcg ctatgggctg gaggcagagg agccttcccg ggtgatcttt
      361 caagcagtca ggcgggagga agtttcgtct gatttccaag ggaggaatta attacgagac
      421 ctcacactgg ctcaccttcg ggtgggcctt tctgcgttta tacctaggag tcaaaattac
      481 tccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct accgacatgg
      541 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
      601 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
      661 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
      721 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
      781 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
      841 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
      901 tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
      961 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     1021 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     1081 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     1141 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     1201 tcattatggt gaaagttgga acctcttacg tgccgatcag tcaattcgag ggggatcaat
     1261 tccgtattga tccggccacg atgcgtccgg cgtagaggat ctgaagatca gcagttcaac
     1321 ctgttgatag tacgtactaa gctctcatgt ttcacgtact aagctctcat gtttaacgta
     1381 ctaagctctc atgtttaacg aactaaaccc tcatggctaa cgtactaagc tctcatggct
     1441 aacgtactaa gctctcatgt ttcacgtact aagctctcat gtttgaacaa taaaattaat
     1501 ataaatcagc aacttaaata gcctctaagg ttttaagttt tataagaaaa aaaagaatat
     1561 ataaggcttt taaagctttt aaggtttaac ggttgtggac aacaagccag ggatgtaacg
     1621 cactgagaag cccttagagc ctct
//
