LOCUS       sequence-405300-        9398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single-color FUCCI4 sensor for S/G2 cell cycle transition and G2/M 
            transition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Qian Y, Celiker OT, Wang Z, Guner-Ataman B, Boyden ES
  TITLE     Temporally multiplexed imaging of dynamic signaling networks in 
            living cells.
  JOURNAL   Cell. 2023 Dec 7;186(25):5656-5672.e21. doi: 
            10.1016/j.cell.2023.11.010. Epub 2023 Nov 28.
  PUBMED    38029746
REFERENCE   2  (bases 1 to 9398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.11.010"; journalName: "Cell"; date: "2023-12-7-
            7"; volume: "186"; issue: "25"; pages: "5656-5672.e21"
COMMENT     Sequence Label: pLL3.7m-YFP-SLBP(18-126)-IRES-H1-Skylan62A
FEATURES             Location/Qualifiers
     source          1..9398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(157..174)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     384..403
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1189..1869
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFAYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     protein_bind    complement(1897..1930)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    1986..2574
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2039..2059)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2457..2468)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2577..2593)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             2816..2996
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(3058..3646)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3138..3157)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3817..4608,4609..4677))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4440..4459
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4678..4782)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4873..5176
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5178..5376
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             5394..5574
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5621..5746
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6243..6476
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6661..6705
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7003..7120
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7179..7491
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     7410..7430
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     7508..7524
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7566..7599)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        7715..8018
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8019..8222
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             join(8258..8260,8261..8263,8264..8974)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8303..8324)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8564..8583)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8911..8932
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(9388..9398,1..562)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
       61 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
      121 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
      181 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
      241 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
      301 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
      361 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
      421 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
      481 catgtgttta gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt
      541 cctttgaaaa acacgatgat aatggccaca gaattcatga ccgagaactc cacctccgcc
      601 ccggcggcga agcccaaacg ggccaaggct tccaagaagt ccacggacca ccccaagtat
      661 tcagacatga tcgtggctgc tatccaggca gagaagaacc gtgccggctc ctcgcgccag
      721 tccatccaaa agtatatcaa gagccactac aaggtgggtg agaacgccga ctcccagatc
      781 aagttgtcca tcaagcgcct agtgaccacc ggtgttctca agcaaaccaa aggggtgggc
      841 gcctcggggt ccttcaggct ggccaagggc gatgagccca aaaggtcggt ggctttcaag
      901 aagaccaaga aggaagtcaa gaaagtggcc actccaaaga aggcagccaa gcccaagaag
      961 gctgcctcca aagccccaag caagaaaccc aaagccaccc ctgtcaagaa ggccaagaag
     1021 aagccggctg ccacgcccaa gaaagccaaa aagcccaagg ttgtcaaagt caaaccagtc
     1081 aaggcctcca aacccaagaa ggccaaaacc gtgaagccca aagccaagtc gagtgccaag
     1141 agggggagca agaagaagtc cggactcaga tctggaggct ccggaggcat gagtgcgatt
     1201 aagccagaca tgaagatcaa actccgtatg gaaggcaacg taaacgggca ccactttgtg
     1261 atcgacggag atggtacagg caagcctttt gagggaaaac agagtatgga tcttgaagtc
     1321 aaagagggcg gacctctgcc ttttgccttt gatatcctga ccactgcatt cgcgtacggc
     1381 aacagggtat tcgccaaata tccagacaac atacaagact attttaagca gtcgtttcct
     1441 aaggggtatt cgtgggaacg aagcttgact ttcgaagacg ggggcatttg caacgccaga
     1501 aacgacataa caatggaagg ggacactttc tataataaag ttcgatttta tggtaccaac
     1561 tttcccgcca atggtccagt tatgcagaag aagacgctga aatgggagcc ctccactgag
     1621 aaaatgtatg tgcgtgatgg agtgctgacg ggtgatgtag agatggcttt gttgcttgaa
     1681 ggaaatgccc attaccgatg tgacttcaga actacttaca aagctaagga gaagggtgtc
     1741 aagttaccag gcgcccactt tgtggaccac tgcattgaga ttttaagcca tgacaaagat
     1801 tacaacaagg ttaagctgta tgagcatgct gttgctcatt ctggattgcc tgacaatgcc
     1861 agacgatgag tttaaacgaa ttcgtcgagg gacctaataa cttcgtatag catacattat
     1921 acgaagttat acatgtttaa gggttccggt tccactaggt acaattcgat atcaagctta
     1981 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     2041 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     2101 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     2161 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     2221 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     2281 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     2341 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     2401 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     2461 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     2521 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     2581 accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     2641 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     2701 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgacgc gtgattttaa
     2761 aagaaaaggg gggactggaa gggctaattc actcccactg ctttttgcct gtactgggtc
     2821 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     2881 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     2941 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     3001 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3061 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3121 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3181 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3241 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3301 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3361 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3421 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3481 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3541 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3601 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3661 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3721 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3781 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3841 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3901 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3961 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4021 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4081 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4141 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4201 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4261 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4321 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4381 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4441 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4501 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4561 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4621 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4681 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     4741 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4801 gccacctgac actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     4861 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     4921 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     4981 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     5041 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     5101 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     5161 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     5221 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     5281 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     5341 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
     5401 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5461 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5521 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     5581 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     5641 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     5701 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     5761 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     5821 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     5881 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     5941 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     6001 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     6061 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
     6121 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     6181 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     6241 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     6301 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     6361 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     6421 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     6481 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     6541 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     6601 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     6661 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     6721 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     6781 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     6841 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     6901 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     6961 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     7021 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     7081 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     7141 cagagatcca gtttggttag taccgggccc gctctagaga tccgacgcgc catctctagg
     7201 cccgcgccgg ccccctcgca cagacttgtg ggagaagctc ggctactccc ctgccccggt
     7261 taatttgcat ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat
     7321 aatctgttct ttttaatact agctacattt tacatgatag gcttggattt ctataagaga
     7381 tacaaatact aaattattat tttaaaaaac agcacaaaag gaaactcacc ctaactgtaa
     7441 agtaattgtg tgttttgaga ctataaatat cccttggaga aaagccttgt taacgcgcgg
     7501 tgaccctcga ggtcgacggt atcgataagc tcgcttcacg agattccagc aggtcgaggg
     7561 acctaataac ttcgtatagc atacattata cgaagttata ttaagggttc caagcttaag
     7621 cggccgcgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg
     7681 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     7741 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     7801 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     7861 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     7921 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     7981 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     8041 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     8101 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     8161 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     8221 ctggtttagt gaaccgtcag atccgctagc accaggtatg gtgagcaagg gcgaggagct
     8281 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     8341 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     8401 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     8461 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     8521 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     8581 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     8641 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     8701 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     8761 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     8821 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
     8881 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     8941 cgggatcact ctcggcatgg acgagctgta caagtactca atgttgcctt ccctgggagg
     9001 atacccatca cactggcgac cactcgaggc cagcccgccg tcccccgcgc gatggagcct
     9061 gggacggaag cgcagagccg acggcaggcg ctggaggccc gaagacgccg aggaggcaga
     9121 gcaccgcggc gccgagcgca gacccgagag ctttaccact cctgaaggcc ctaaaccccg
     9181 ttccagatgc tctgactggg caagtgcagt tgaagaagat gaaatgagga ccagagttaa
     9241 caaagaaatg gcaagatata aaaggaaact cctcatcaat gactttggaa gagagagaaa
     9301 atcatcatca ggaagttctg attcaaagga gtctatgtct actgtgccgg ctgacgatcg
     9361 aggatgaaga aggatcttag cttaaggccc ctctccct
//
