LOCUS       sequence-405311-       11302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid of en31FnCas9ABEmax8.17d base editor 
            with T2A-EGFP and cloning backbone for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11302)
  AUTHORS   Acharya S, Ansari AH, Kumar Das P, Hirano S, Aich M, Rauthan R, 
            Mahato S, Maddileti S, Sarkar S, Kumar M, Phutela R, Gulati S, 
            Rahman A, Goel A, Afzal C, Paul D, Agrawal T, Pulimamidi VK, Jalali 
            S, Nishimasu H, Mariappan I, Nureki O, Maiti S, Chakraborty D
  TITLE     PAM-flexible Engineered FnCas9 variants for robust and ultra-precise
            genome editing and diagnostics.
  JOURNAL   Nat Commun. 2024 Jun 28;15(1):5471. doi: 10.1038/s41467-024-49233-w.
  PUBMED    38942756
REFERENCE   2  (bases 1 to 11302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-06-28"; volume: "15(1):5471. doi"; pages: " 
            10.1038/s41467-024-49233-w"
COMMENT     Sequence Label: PX458-3xHA-en31FnCas9ABEmax8.17d
FEATURES             Location/Qualifiers
     source          1..11302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        268..340
                     /label=Fn gRNA scaffold
                     /note="guide RNA scaffold for the Francisella novicida 
                     CRISPR/Cas9 system (Hirano et al., 2016)"
     enhancer        437..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          997..1224
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1236..1245
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1284..1304
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2493..7376
                     /codon_start=1
                     /product="Cas9 endonuclease from the Francisella novicida 
                     Type II CRISPR/Cas system (Hirano et al., 2016)"
                     /label=FnCas9
                     /translation="NFKILPIAIALGVKNTGVFSAFYQKGTSLERLDNKNGKVYELSKD
                     SYTLLMNNRTARRHQRRGIDRKQLVKRLFKLIWTEQLNLEWDKDTQQAISFLFNRRGFS
                     FITDGYSPEYLNIVPEQVKAILMDIFDDYNGEDDLDSYLKLATEQESKISEIYNKLMQK
                     ILEFKLMKLCTDIKDDKVSTKTLKEITSYEFELLADYLANYSESLKTQKFSYTDKQGNL
                     KELSYYHHDKYNIQEFLKRHATINDRILDTLLTDDLDIWNFNFEKFDFDKNEEKLQNQE
                     DKDHIQAHLHHFVFAVNKIKSEMASGGRHRSQYFQEITNVLDENNHQEGYLKNFCENLH
                     NKKYSNLSVKNLVNLIGNLSNLELKPLRKYFNDKIHAKADHWDEQKFTETYCHWILGEW
                     RVGVKDQDKKDGAKYSYKDLCNELKQKVTKAGLVDFLLELDPCRTIPPYLDNNNRKPPK
                     CQSLILNPKFLDNQYPNWQQYLQELKKLQSIQNYLDSFETDLKVLKSSKDQPYFVEYKS
                     SNQQIASGQRDYKDLDARILQFIFDRVKASDELLLNEIYFQAKKLKQKASSELEKLESS
                     KKLDEVIANSQLSQILKSQHTNGIFEQGTFLHLVCKYYKQRQRARDSRLYIMPEYRYDK
                     KLHKYNNTGRFDDDNQLLTYCNHKPRQKRYQLLNDLAGVLQVSPNFLKDKIGSDDDLFI
                     SKWLVEHIRGFKKACEDSLKIQKDNRGLLNHKINIARNTKGKCEKEIFNLICKIEGSED
                     KKGNYKHGLAYELGVLLFGEPNEASKPEFDRKIKKFNSIYSFAQIQQIAFAERKGNANT
                     CAVCSADNAHRMQQIKITEPVEDNKDKIILSAKAQRLPAIPTRIVDGAVKKMATILAKN
                     IVDDNWQNIKQVLSAKHQLHIPIITESNAFEFEPALADVKGKSLKDRRKKALERISPEN
                     IFKDKNNRIKEFAKGISAYSGANLTDGDFDGAKEELDHIIPRSHKKYGTLNDEANLICV
                     TRGDNKNKGNRIFCLRDLADNYKLKQFETTDDLEIEKKIADTIWDANKKDFKFGNYRSF
                     INLTPQEQKAFRHALFLADENPIKQAVIRAINNRNRTFVNGTQRYFAEVLANNIYLRAK
                     KENLNTDKISFDYFGIPTIGNGRGIAEIRQLYEKVDSDIQAYAKGDKPQASYSHLIDAM
                     LAFCIAADEHRNDGSIGLEIDKNYSLYPLDKNTGEVFTKDIFSQIKITDNEFSDKKLVR
                     KKAIEGFNTHRQMTRDTIYAENYLPILIHKELNEVRKGYTWKNSEEIKIFKGKKYDIQQ
                     LNNLVYCLKFVDKPISIDIQISTLEELRNILTTNNIAATAEYYYINLKTQKLHEYYIEN
                     YNTALGYKKYSKEMEFLRSLAYRSRRVKIKSIDDVKQVLDKDSNFIIGKITLPFKKEWQ
                     RLYREWQNTTIKDDYEFLKSFFNVKSITKLHKKVRKDFSLPISTNHGKFLVKRKTWDNN
                     FIYQILNDSDSRADGTKPFIPAFDISKNEIVEAIIDSFTSKNIFWLPKNIELQKVDNKN
                     IFAIDTSKWFEVETPSDLRDIGIATIQYKIDNNSRPKVRVKLDYVIDDDSKINYFMNHS
                     LLKSRYPDKVLEILKQSTIIEFESSGFNKTIKEMLGMKLAGIYNETSNN"
     CDS             7419..7439
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7452..7532
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             7548..7601
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7602..7604,7605..8315)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(7602..7604,7605..8315)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7644..7665)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7905..7924)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8252..8273
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8343..8360)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8349..8556
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8565..8705
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   8565..8694
                     /label=AAV2 ITR
     rep_origin      8780..9235
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8867..8886)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9077..9098
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9252..9271)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9371..9393
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9431..9449)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9517..9621
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9622..9690,9691..10482)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9840..9859)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10653..11241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11142..11161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg ggtcttcgag aagacctgtt tcagttgcgc cgaaaggcgc tctgtaatca
      301 tttaaaagta ttttgaacgg acctctgttt gacacgtctg ttttttgttt tagagctaga
      361 aatagcaagt taaaataagg ctagtccgtt tttagcgcgt gcgccaattc tgcagacaaa
      421 tggctctaga ggtacccgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      481 acgacccccg cccattgacg tcaatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattgt gcccagtaca
      661 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc
      781 caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg gggcggggcg
      841 gggcgagggg cggggcgggg cgaggcggag gggcggggcg gggcgaggcg gagaggtgcg
      901 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      961 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
     1021 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
     1081 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gctgagcaag
     1141 aggtaagggt ttaagggatg gttggttggt ggggtattaa tgtttaatta cctggagcac
     1201 ctgcctgaaa tcactttttt tcaggttgga ccggtgccac catgggatcc aaacggacag
     1261 ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtctctgaa gtcgagttta
     1321 gccacgagta ttggatgagg cacgcactga ccctggcaaa gcgagcatgg gatgaaagag
     1381 aagtccccgt gggcgccgtg ctggtgcaca acaatagagt gatcggagag ggatggaaca
     1441 ggccaatcgg ccgccacgac cctaccgcac acgcagagat catggcactg aggcagggag
     1501 gcctggtcat gcagaattac cgcctgatcg atgccaccct gtatgtgaca ctggagccat
     1561 gcgtgatgtg cgcaggagca atgatccaca gcaggatcgg aagagtggtg ttcggagcac
     1621 gggacgccaa gaccggcgca gcaggctccc tgatggatgt gctgcaccac cccggcatga
     1681 accaccgggt ggagatcaca gagggaatcc tggcagacga gtgcgccgcc ctgctgagcg
     1741 atttctttag aatgcggaga caggagatca aggcccagaa gaaggcacag agctccaccg
     1801 actctggagg atctagcgga ggatcctctg gaagcgagac accaggcaca agcgagtccg
     1861 ccacaccaga gagctccggc ggctcctccg gaggatcctc tgaggtggag ttttcccacg
     1921 agtactggat gagacatgcc ctgaccctgg ccaagagggc acgcgatgag agggaggtgc
     1981 ctgtgggagc cgtgctggtg ctgaacaata gagtgatcgg cgagggctgg aacagagcca
     2041 tcggcctgca cgacccaaca gcccatgccg aaattatggc cctgagacag ggcggcctgg
     2101 tcatgcagaa ctacagactg attgacgcca ccctgtacag cacattcgag ccttgcgtga
     2161 tgtgcgccgg cgccatgatc cactctagga tcggccgcgt ggtgtttggc tggaggaacg
     2221 caaaaaccgg cgccgcaggc tccctgatgg acgtgctgca ctaccccggc atgaatcacc
     2281 gcgtcgaaat taccgaggga atcctggcag atgaatgtgc cgccctgctg tgctatttct
     2341 ttcggatgcc tagaagggtg ttcaatgctc agaagaaggc ccagagctcc accgactccg
     2401 gaggatctag cggaggctcc tctggctctg agacacctgg cacaagcgag agcgcaacac
     2461 ctgaaagcag cgggggcagc agcggggggt caaattttaa gatcctgcct atcgcaatcg
     2521 ccctgggcgt caagaatact ggcgtgttta gtgcttttta tcagaagggg acctcactgg
     2581 agagactgga caataagaac ggaaaagtgt atgaactgtc caaggattct tacactctgc
     2641 tgatgaacaa taggaccgca cggagacacc agaggcgagg aattgacagg aaacagctgg
     2701 tgaagcgcct gttcaaactg atctggacag agcagctgaa cctggaatgg gataaggaca
     2761 ctcagcaggc catcagcttc ctgtttaatc gacggggatt ctcttttatt actgacggct
     2821 atagtcctga gtacctgaac atcgtgccag aacaggtcaa ggcaatcctg atggacattt
     2881 tcgacgatta taatggcgag gacgatctgg attcctacct gaaactggcc acagagcaag
     2941 agagtaagat cagcgaaatc tacaacaagc tgatgcagaa gatcctggag ttcaagctga
     3001 tgaaactgtg caccgacatc aaggacgata aagtgagtac caagacactg aaagagatca
     3061 caagctacga gttcgaactg ctggccgatt atctggctaa ctacagcgaa tccctgaaaa
     3121 cccagaaatt ttcctacaca gacaagcagg gcaatctgaa agagctgtct tactaccacc
     3181 atgataagta caacatccag gagttcctga agagacacgc caccatcaat gacaggattc
     3241 tggatacact gctgactgac gatctggaca tctggaactt caacttcgag aagttcgatt
     3301 tcgacaagaa cgaggaaaaa ctgcagaatc aggaagataa ggaccacatt caggctcatc
     3361 tgcaccattt cgtgtttgca gtcaataaga tcaaaagcga gatggcatcc ggcgggcgcc
     3421 atcgaagcca gtacttccag gaaatcacca acgtgctgga cgagaacaat caccaggaag
     3481 gctacctgaa aaacttctgt gagaatctgc ataacaagaa gtacagcaat ctgtccgtga
     3541 agaatctggt caacctgatt ggaaatctgt ccaacctgga actgaagccc ctgcgcaaat
     3601 acttcaacga caagatccac gctaaagcag accattggga tgagcagaag tttactgaaa
     3661 cctattgcca ctggattctg ggcgagtggc gggtgggggt caaggatcag gacaagaaag
     3721 acggcgcaaa gtattcttac aaggacctgt gtaacgagct gaagcagaaa gtgactaagg
     3781 ccgggctggt ggacttcctg ctggagctgg acccctgccg aaccattcca ccttacctgg
     3841 acaacaataa cagaaagcca cccaaatgtc agagcctgat cctgaatccc aagtttctgg
     3901 ataatcagta tcctaactgg cagcagtacc tgcaggagct gaagaaactg cagtcaatcc
     3961 agaactacct ggacagcttc gaaaccgatc tgaaggtgct gaaaagctcc aaggaccagc
     4021 cttacttcgt cgagtacaag tctagtaacc agcagatcgc ttccggccag cgggattaca
     4081 aggatctgga cgcaagaatc ctgcagttca tttttgacag ggtgaaggcc tctgatgagc
     4141 tgctgctgaa cgaaatctat ttccaggcaa agaaactgaa gcagaaagcc tcaagcgagc
     4201 tggaaaagct ggagtcctct aagaaactgg acgaagtgat cgctaactct cagctgagtc
     4261 agattctgaa gtctcagcac acaaatggaa tcttcgagca gggcactttt ctgcatctgg
     4321 tgtgcaaata ctataagcag cgacagagag ccagggacag ccgcctgtac atcatgcctg
     4381 aatatcgata cgataagaaa ctgcacaagt acaacaacac cggccgcttt gacgatgaca
     4441 accagctgct gacatattgt aatcataagc cccggcagaa aagataccag ctgctgaacg
     4501 acctggcagg agtgctgcag gtctctccta attttctgaa ggataaaatc gggtccgatg
     4561 acgatctgtt catttctaag tggctggtgg agcacatccg gggctttaag aaggcctgcg
     4621 aagatagcct gaaaatccag aaggataaca ggggactgct gaatcataag atcaacattg
     4681 cacgcaatac caagggcaaa tgcgagaaag aaatcttcaa cctgatctgt aagattgagg
     4741 ggagcgaaga taagaaaggg aattataagc acggactggc ctacgagctg ggagtgctgc
     4801 tgttcggaga gccaaacgag gccagcaagc ccgaatttga taggaaaatc aagaaattca
     4861 attcaatcta cagctttgcc cagatccagc agattgcctt tgctgagagg aaggggaatg
     4921 caaacacatg cgccgtgtgt agtgcagaca acgcccatcg catgcagcag atcaaaatta
     4981 ctgagccagt cgaagataat aaggataaaa tcattctgtc agcaaaggca cagcgactgc
     5041 ctgcaatccc aacccgaatt gtggatggag ctgtcaagaa aatggctaca attctggcaa
     5101 agaatatcgt ggacgataat tggcagaaca ttaagcaggt cctgagcgca aaacaccagc
     5161 tgcatatccc aatcattacc gagtccaacg ccttcgagtt tgaacccgct ctggcagacg
     5221 tgaagggcaa atctctgaag gatagaagga agaaagccct ggagcgaatt agtcccgaaa
     5281 acatcttcaa ggataagaac aacagaatca aggagtttgc taaggggatt tccgcctact
     5341 ctggagctaa cctgacagat ggggacttcg atggagcaaa ggaggaactg gatcacatca
     5401 ttcctcgcag ccataagaaa tatggcactc tgaacgacga ggctaatctg atttgcgtga
     5461 cccggggcga taataagaac aaagggaacc ggatcttctg tctgagagac ctggccgata
     5521 attacaagct gaaacagttt gagaccacag acgatctgga gatcgaaaag aaaattgccg
     5581 acaccatctg ggatgctaat aagaaggact tcaagttcgg aaactatcgg agcttcatca
     5641 atctgacacc tcaggagcag aaagcattca gacacgccct gtttctggct gatgaaaacc
     5701 caatcaagca ggcagtgatc agagccatta ataaccgcaa ccgaaccttc gtgaatggca
     5761 cacagaggta ttttgctgag gtcctggcaa ataacatcta cctgcgcgcc aagaaagaaa
     5821 atctgaacac tgacaagatc agcttcgatt actttggaat ccctaccatt ggaaacggcc
     5881 gagggatcgc tgagattcgg cagctgtatg aaaaggtgga cagtgatatc caggcctacg
     5941 ctaaaggcga caagccacag gcctcttata gtcacctgat tgatgctatg ctggcattct
     6001 gcatcgccgc tgacgagcat cggaacgatg gatctattgg cctggaaatc gacaaaaact
     6061 atagtctgta ccctctggat aagaatactg gcgaggtgtt caccaaagac atcttttcac
     6121 agatcaagat taccgacaac gagttcagcg ataagaaact ggtcagaaag aaagctattg
     6181 aagggtttaa cacacacaga cagatgacta gggataccat ctatgcagag aattacctgc
     6241 ctatcctgat tcataaggag ctgaacgaag tgaggaaggg gtacacatgg aaaaattccg
     6301 aggaaatcaa aattttcaag ggaaagaaat acgacatcca gcagctgaat aacctggtgt
     6361 attgtctgaa gtttgtggac aaaccaatca gtattgatat ccagatttca accctggagg
     6421 aactgagaaa catcctgact accaataaca ttgcagccac tgccgagtac tattacatta
     6481 atctgaaaac ccagaagctg cacgagtatt acatcgaaaa ttacaacaca gccctggggt
     6541 ataagaaata cagcaaggag atggagttcc tgaggtccct ggcttatagg tctaggcgcg
     6601 tgaagatcaa aagtattgac gatgtcaagc aggtcctgga caaggattca aacttcatca
     6661 tcggaaagat cacactgccc ttcaagaaag agtggcagcg actgtaccgg gaatggcaga
     6721 acacaactat caaagacgat tatgagtttc tgaagagctt ctttaatgtg aagtccatta
     6781 ctaaactgca caagaaagtc cggaaagact tctctctgcc catcagtaca aaccacggca
     6841 agtttctggt gaagagaaaa acttgggata ataacttcat ctaccagatt ctgaatgact
     6901 cagatagcag ggcagacggg actaaaccct tcattcctgc ctttgatatc agcaagaacg
     6961 agattgtgga agccatcatt gacagtttca cctcaaaaaa catcttttgg ctgccaaaga
     7021 atattgagct gcagaaggtg gacaacaaga acatcttcgc cattgatacc agcaagtggt
     7081 ttgaggtcga aacaccatcc gacctgcgcg atatcggcat tgctaccatt cagtacaaga
     7141 tcgacaataa ctcacgcccc aaggtgcgag tcaaactgga ttacgtgatc gacgatgaca
     7201 gcaagattaa ctatttcatg aatcactcac tgctgaagag ccggtatccc gacaaagtcc
     7261 tggagatcct gaagcagagc acaatcattg agttcgaaag ttcagggttt aacaaaacta
     7321 ttaaggagat gctgggaatg aagctggccg gcatctacaa tgaaacctcc aataactctg
     7381 gcggctcaaa aagaaccgcc gacggcagcg agttcgagcc caagaagaag aggaaagtca
     7441 gcggcggcag ctacccatac gatgttccag attacgctta cccatacgat gttccagatt
     7501 acgcttaccc atacgatgtt ccagattacg ctgaattcgg cagtggagag ggcagaggaa
     7561 gtctgctaac atgcggtgac gtcgaggaga atcctggccc agtgagcaag ggcgaggagc
     7621 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     7681 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     7741 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     7801 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     7861 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     7921 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     7981 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     8041 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     8101 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     8161 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     8221 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     8281 ccgggatcac tctcggcatg gacgagctgt acaaggagtt ctaactagag ctcgctgatc
     8341 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     8401 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     8461 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     8521 ggaggattgg gaagagaata gcaggcatgc tggggagcgg ccgcaggaac ccctagtgat
     8581 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
     8641 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct
     8701 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     8761 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     8821 gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc
     8881 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     8941 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     9001 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9061 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg
     9121 ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag
     9181 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg
     9241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     9301 acacccgctg acgcgccctg acgggcttgt ctgctcccag catccgctta cagacaagct
     9361 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     9421 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     9481 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     9541 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     9601 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     9661 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     9721 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     9781 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     9841 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     9901 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     9961 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    10021 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    10081 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    10141 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    10201 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    10261 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    10321 ggagccggtg agcgtggaag ccgcggtatc attgcagcac tggggccaga tggtaagccc
    10381 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    10441 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    10501 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    10561 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    10621 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    10681 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    10741 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    10801 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    10861 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    10921 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    10981 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    11041 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    11101 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    11161 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    11221 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    11281 tgctggcctt ttgctcacat gt
//
