LOCUS       sequence-405312-       13209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the expression of geneticin resistance and 
            Nanoluciferase, and the expression of firefly luciferase under the 
            Ppaf promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13209)
  AUTHORS   Gandia M, Moreno-Gimenez E, Giner-Llorca M, Garrigues S, 
            Ropero-Perez C, Locascio A, Martinez-Culebras PV, Marcos JF, 
            Manzanares P
  TITLE     Development of a FungalBraid Penicillium expansum-based expression 
            system for the production of antifungal proteins in fungal 
            biofactories.
  JOURNAL   Microb Biotechnol. 2022 Feb;15(2):630-647. doi: 
            10.1111/1751-7915.14006. Epub 2022 Jan 27.
  PUBMED    35084102
REFERENCE   2  (bases 1 to 13209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/1751-7915.14006"; journalName: "Microb Biotechnol"; date: 
            "2022-02"; volume: "15"; issue: "2"; pages: "630-647"
COMMENT     Sequence Label: FB323
FEATURES             Location/Qualifiers
     source          1..13209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(287..1081)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(398..417)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1008..1027
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             4933..6585
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4999..5017)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    6858..6882
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8183..8812
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9241..10314
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10380..10574
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10810..10832)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10918..11058
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10973..10992
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11073..11090)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11244..11832)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11324..11343)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11920..12735)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(11920..12735)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12643..12662
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    13168..13192
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagagcgtc atcatgcaac atgcatgtac tgtctgatgt attaagagta taggggtcaa
       61 caactagagt gtctgacagg tatatctagc agtttgattt gaccattctt ttattaaaac
      121 actgtaaccc gcaacgaaga actcctccgc ttcaagtcaa gaatgcgcat atgtgcgtaa
      181 aatcgacaag cccggcggca ttccaccgag cagggtattg cctttggcac tttacgtgcg
      241 cagaggagcc tgaatgttga gtggaatgat ggtggaatga tgaagctcag aagaactcgt
      301 caagaaggcg atagaaggct atgcgctgcg aatcgggagc ggcgataccg taaagcacga
      361 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
      421 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
      481 cattttccac catgatattc ggcaagcagg cgtcgccgtg ggtcacgacg agatcctcgc
      541 cgtcgggcat ccgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
      601 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
      661 tgcggtgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
      721 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
      781 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
      841 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
      901 ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
      961 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     1021 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     1081 ttttggatgc ttgggtagaa taggtaagtc agattgaatc tgaaataaag ggaggaaggg
     1141 cgaacttaag aaggtatgac cgggtcgtcc acttaccttg cttgacaaac gcaccaagtt
     1201 atcgtgcacc aagcagcaga tgataataat gtcctcgttc ctgtctgcta ataagagtca
     1261 cacttcgagc gccgccgcta ctgctacaag tggggctgat ctgaccagtt gcctaaatga
     1321 accatcttgt caaacgacac aaattttgtg ctcaccgcct ggacgactaa accaaaatag
     1381 gcattcattg ttgacctcca ctagctccag ccaagcccaa aaaatgctcc ttcaatatca
     1441 gttctcctga cggagtggcg catgcggaca gacggacgga cgcagagaga agggctgagt
     1501 aataagcgcc actgcgccag acagctctgg cggctctgag gtgcagtgga tgattattaa
     1561 tccgggaccg gccgcccctc cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca
     1621 agaatctatt gcatcatcgg agaatatgga gcttcatcga atcaccggca gtaagcgaag
     1681 gagaatgtga agccaggggt gtatagccgt cggcgaaata gcatgccatt aacctaggta
     1741 cagaagtcca attgcttccg atctggtaaa agattcacga gatagtacct tctccgaagt
     1801 aggtagagcg agtacccggc gcgtaagctc cctaattggc ccatccggca tctgtagggc
     1861 gtccaaatat cgtgcctctc ctgctttgcc cggtgtatga aaccggaaag gccgctcagg
     1921 agctggccag cggcgcagac cgggaacaca agctggcagt cgacccatcc ggtgctctgc
     1981 actcgacctg ctgaggtccc tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg
     2041 gtgtgtcggc ggggttgaca aggtcgttgc gtcagtccaa catttgttgc catattttcc
     2101 tgctctcccc accagctgct cttttctttt ctctttcttt tcccatcttc agtatattca
     2161 tcttcccatc caagaacctt taaaatggtt ttcacccttg aagatttcgt cggcgactgg
     2221 cgccaaaccg ccggatataa ccttgaccag gtgctggagc agggaggagt ctcttccttg
     2281 ttccagaatc tcggcgtttc cgttactcct atccagcgta ttgtgttgag cggagagaac
     2341 ggtcttaaga ttgacatcca cgtcattatc ccatacgagg gcctctctgg cgatcaaatg
     2401 ggacagatcg agaaaatttt caaggtcgtt tatccagtcg acgatcatca ctttaaagtt
     2461 atcctgcact acggtactct tgtgatcgat ggcgtcaccc ccaacatgat tgactacttc
     2521 ggccgaccct acgagggtat cgctgtgttc gacggtaaga agattactgt gaccggtact
     2581 ttgtggaatg gaaacaagat tatcgatgag cgcctcatca accctgatgg ctccttgctg
     2641 tttcgtgtta ccattaacgg agttactggt tggcgccttt gcgaacgtat tctcgcataa
     2701 gcttcatcat tccaccatca ttccactcaa cattcaggct cctctgcgca cgtaaagtgc
     2761 caaaggcaat accctgctcg gtggaatgcc gccgggcttg tcgattttac gcacatatgc
     2821 gcattcttga cttgaagcgg aggagttctt cgttgcgggt tacagtgttt taataaaaga
     2881 atggtcaaat caaactgcta gatatacctg tcagacactc tagttgttga cccctatact
     2941 cttaatacat cagacagtac atgcatgttg catgatgacg ctctccgtca ggagtggcta
     3001 ttgttgttat catcaaaatc tatattgata ctattatact cgatatgtta tagactttat
     3061 taaaaaaaac tttgtttgtt tgcagaaagt ggatttttaa tcaaatttaa ttgtgatgat
     3121 tacaatgtca attggttatg caagatatta attaatatgt ttaagatttt tcacttttaa
     3181 taagtaaaat aaaatacgtc aaaaaaattt cctaaagata atgccaaaaa ggttaaatta
     3241 tagaaattaa actaaaatat attaaaagtt gacttaaaaa tgcaatgttg accaccgacc
     3301 taggggaaaa caaatttacc gtatctggga tagaatctct tctgtatcat ctcagataaa
     3361 gattactaca taaacacact ttacagtttt acgacgccca aaattcaatt cccggagatt
     3421 aaaaacccta aaacaaacac tggcgattcg attttccctc ccaattcaaa tcatctctta
     3481 atcttcttct tctcactatc ctctcaaatt cgccgccgat cttccatcaa ttttgatttc
     3541 tcgcaccgaa atttcgactt ttcatctcct atcgacgaaa ccctaatttt tcacactcca
     3601 gaaatcgaac tttgacgacg atgttaatgg ctaaaccccg ctgtcaggag gaattcagag
     3661 agcttttcgt acgaagtgcg taatgtacgt agcattttat ggtagcatgc aaagcacatt
     3721 ttgctgcaac cccaatttaa tgcggtcctg ctcaataatt gatctgcact aaggccttgg
     3781 cgatggggcc agaaaagggt tgttcagtgg tgtgtactcc gtaatggtca agccgatttc
     3841 gagaatgacc gtagtgttca ttcatcagtg cgatattaaa tcagttagct actctatctg
     3901 aaagctaata aatttcttta ccactaacaa tactcttctc tgactgaaag taccttttcc
     3961 actcccctca tacttcatgt tttaagctca accgtaggaa agcctgtata tcttaaaaga
     4021 tttggattta ctcttccagc gcttactgtc tgctctttcg gccgagcgaa ccttggcagt
     4081 atgatcggac tatgtacttt gttacacaaa aggagaagcg gggctgccac tgaggacaac
     4141 ccctgttcaa gggctagcat cccgctgtaa gcccacccat cccaccttga agtatgcaac
     4201 ttttgaccgc ctagaccatg tgagcttatg ttactgaaat actacccgcg aatcatttcc
     4261 taatttgctt tggctcgaat ccaccccagc cctacgtaac acaaccggga gctgccttac
     4321 agcttggctg tatcacagta tcacatagat acatacatag tatagtgcct ttgccttttc
     4381 gacctataag catccgccat atgctaaacc ttctcatata ccaacatttt ggatttggag
     4441 atcatttcct agtgaaacaa ctttatcaaa tgcaatgcag ccatcgtcct ttgcagatcc
     4501 gagtggccca gtcaccgtgt caacgtgtca gccgttttct ctgcttttta ggaaatgatt
     4561 accactaggt aagcccaaaa atatcttcct ggtaaacaag tagtgcatct taccccggag
     4621 gctgaagcag gtaagggatt ttggagagtc cccacccgta agaatatacc agccaagagg
     4681 tccagtatcc tgaagtatgt gaggcattaa tgtcattgga gaagtcatgc aatccataag
     4741 ctgccacccc caagatgact gcattggacc tgagcattgt atgtgtcacc tttcacacag
     4801 agctcatgat ctggtttata aaggcggctt catgaccctc aattccatat agtatcactc
     4861 ccatcacagc atttcgatat cttcaaccac tttaaccttc tccagaggat catcatctca
     4921 agcccttcat aaatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg
     4981 ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt
     5041 cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac
     5101 ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac
     5161 agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta
     5221 tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac
     5281 agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt
     5341 ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg
     5401 gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt
     5461 aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg
     5521 aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc
     5581 gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg
     5641 attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg
     5701 atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc
     5761 cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc
     5821 aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc
     5881 gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc
     5941 aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat
     6001 gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg
     6061 gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg
     6121 attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga
     6181 tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac
     6241 cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc
     6301 atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac
     6361 gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa
     6421 gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt
     6481 gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga
     6541 gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaatgagc ttacttaacg
     6601 ttactgaaat catcaaacag cttgacgaat ctggatataa gatcgttggt gtcgatgtca
     6661 gctccggagt tgagacaaat ggtgttcagg atctcgataa gatacgttca tttgtccaag
     6721 cagcaaagag tgccttctag tgatttaata gctccatgtc aacaagaata aaacgcgttt
     6781 cgggtttacc tcttccagat acagctcatc tgcaatgcat cgaccgtctg agacatgcgc
     6841 tgtcatgaga cgaattctga caggatatat tggcgggtaa acctaagaga aaagagcgtt
     6901 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
     6961 tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc
     7021 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt catctgaaaa cgacatgtcg
     7081 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
     7141 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
     7201 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
     7261 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
     7321 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
     7381 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
     7441 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
     7501 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
     7561 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga
     7621 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
     7681 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
     7741 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
     7801 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
     7861 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
     7921 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
     7981 acctctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
     8041 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
     8101 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
     8161 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
     8221 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
     8281 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
     8341 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
     8401 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
     8461 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
     8521 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
     8581 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
     8641 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
     8701 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
     8761 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
     8821 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
     8881 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
     8941 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
     9001 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
     9061 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
     9121 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
     9181 cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
     9241 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
     9301 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
     9361 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
     9421 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
     9481 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
     9541 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
     9601 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
     9661 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
     9721 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
     9781 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
     9841 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
     9901 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
     9961 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
    10021 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
    10081 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
    10141 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
    10201 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
    10261 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
    10321 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg actctttcct
    10381 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
    10441 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
    10501 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
    10561 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
    10621 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
    10681 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
    10741 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
    10801 cggtgaaaac ctctgacaca tgcagctccc ggtgacggtc acagcttgtc tgtaagcgga
    10861 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
    10921 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
    10981 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
    11041 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    11101 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    11161 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    11221 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    11281 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    11341 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    11401 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    11461 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    11521 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    11581 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    11641 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
    11701 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    11761 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    11821 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    11881 aactcacgtt aagggatttt ggtcatgcat tctaggtgat tagaaaaact catcgagcat
    11941 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
    12001 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
    12061 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
    12121 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
    12181 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
    12241 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gtcgaaatac
    12301 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
    12361 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
    12421 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
    12481 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
    12541 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
    12601 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
    12661 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
    12721 ttgaatatgg ctcataacag aacttattat ttccttcctc ttttctacag tatttaaaga
    12781 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
    12841 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
    12901 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
    12961 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
    13021 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
    13081 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
    13141 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acataacgaa
    13201 ttcgtctca
//
