LOCUS       sequence-405313-        7903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the expression of Nanoluciferase (Nluc) under the PgpdA 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7903)
  AUTHORS   Gandia M, Moreno-Gimenez E, Giner-Llorca M, Garrigues S, 
            Ropero-Perez C, Locascio A, Martinez-Culebras PV, Marcos JF, 
            Manzanares P
  TITLE     Development of a FungalBraid Penicillium expansum-based expression 
            system for the production of antifungal proteins in fungal 
            biofactories.
  JOURNAL   Microb Biotechnol. 2022 Feb;15(2):630-647. doi: 
            10.1111/1751-7915.14006. Epub 2022 Jan 27.
  PUBMED    35084102
REFERENCE   2  (bases 1 to 7903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/1751-7915.14006"; journalName: "Microb Biotechnol"; date: 
            "2022-02"; volume: "15"; issue: "2"; pages: "630-647"
COMMENT     Sequence Label: FB312
FEATURES             Location/Qualifiers
     source          1..7903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1544..1568
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2869..3498
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3927..5000
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5066..5260
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5496..5518)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5604..5744
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5659..5678
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5759..5776)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5930..6518)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6010..6029)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6606..7421)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6606..7421)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7329..7348
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    7854..7878
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tcatcatgca acatgcatgt actgtctgat gtattaagag tataggggtc aacaactaga
       61 gtgtctgaca ggtatatcta gcagtttgat ttgaccattc ttttattaaa acactgtaac
      121 ccgcaacgaa gaactcctcc gcttcaagtc aagaatgcgc atatgtgcgt aaaatcgaca
      181 agcccggcgg cattccaccg agcagggtat tgcctttggc actttacgtg cgcagaggag
      241 cctgaatgtt gagtggaatg atggtggaat gatgaagctt atgcgagaat acgttcgcaa
      301 aggcgccaac cagtaactcc gttaatggta acacgaaaca gcaaggagcc atcagggttg
      361 atgaggcgct catcgataat cttgtttcca ttccacaaag taccggtcac agtaatcttc
      421 ttaccgtcga acacagcgat accctcgtag ggtcggccga agtagtcaat catgttgggg
      481 gtgacgccat cgatcacaag agtaccgtag tgcaggataa ctttaaagtg atgatcgtcg
      541 actggataaa cgaccttgaa aattttctcg atctgtccca tttgatcgcc agagaggccc
      601 tcgtatggga taatgacgtg gatgtcaatc ttaagaccgt tctctccgct caacacaata
      661 cgctggatag gagtaacgga aacgccgaga ttctggaaca aggaagagac tcctccctgc
      721 tccagcacct ggtcaaggtt atatccggcg gtttggcgcc agtcgccgac gaaatcttca
      781 agggtgaaaa ccattttaaa ggttcttgga tgggaagatg aatatactga agatgggaaa
      841 agaaagagaa aagaaaagag cagctggtgg ggagagcagg aaaatatggc aacaaatgtt
      901 ggactgacgc aacgaccttg tcaaccccgc cgacacaccg ggcggacaga cggggcaaag
      961 ctgcctacca gggactgagg gacctcagca ggtcgagtgc agagcaccgg atgggtcgac
     1021 tgccagcttg tgttcccggt ctgcgccgct ggccagctcc tgagcggcct ttccggtttc
     1081 atacaccggg caaagcagga gaggcacgat atttggacgc cctacagatg ccggatgggc
     1141 caattaggga gcttacgcgc cgggtactcg ctctacctac ttcggagaag gtactatctc
     1201 gtgaatcttt taccagatcg gaagcaattg gacttctgta cctaggttaa tggcatgcta
     1261 tttcgccgac ggctatacac ccctggcttc acattctcct tcgcttactg ccggtgattc
     1321 gatgaagctc catattctcc gatgatgcaa tagattcttg gtcaacgagg ggcacaccag
     1381 cctttccact tcggggcgga ggggcggccg gtcccggatt aataatcatc cactgcacct
     1441 cagagccgcc agagctgtct ggcgcagtgg cgcttattac tcagcccttc tctctgcgtc
     1501 cgtccgtctg tccgcatgcg ccactccgtc atgagacgga tcctgacagg atatattggc
     1561 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
     1621 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
     1681 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
     1741 agccgtcatc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
     1801 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
     1861 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
     1921 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
     1981 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
     2041 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
     2101 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
     2161 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
     2221 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
     2281 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     2341 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     2401 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     2461 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     2521 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     2581 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     2641 cgggtacgtg ttcgagccgc ccgcgcacct ctcaaccgtg cggctgcatg aaatcctggc
     2701 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     2761 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     2821 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     2881 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     2941 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     3001 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     3061 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     3121 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     3181 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     3241 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     3301 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     3361 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     3421 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     3481 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     3541 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     3601 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     3661 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     3721 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     3781 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     3841 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
     3901 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     3961 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     4021 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     4081 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     4141 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     4201 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     4261 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
     4321 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
     4381 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
     4441 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
     4501 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
     4561 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
     4621 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
     4681 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
     4741 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
     4801 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
     4861 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
     4921 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
     4981 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
     5041 aaaaaggtcg aaaaggactc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     5101 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     5161 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
     5221 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
     5281 ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
     5341 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
     5401 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
     5461 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggtg
     5521 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     5581 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     5641 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     5701 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     5761 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5821 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5881 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5941 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6001 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6061 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6121 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6181 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6241 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6301 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6361 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6421 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6481 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6541 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
     6601 ggtgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
     6661 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
     6721 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     6781 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     6841 acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca
     6901 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
     6961 gattgcgcct gagcgagtcg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
     7021 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     7081 ggatattctt ctaatacctg gaatgctgtt ttccctggga tcgcagtggt gagtaaccat
     7141 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
     7201 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
     7261 agaaacaact ctggcgcatc gggcttccca tacaatcggt agattgtcgc acctgattgc
     7321 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
     7381 cgcggccttg agcaagacgt ttcccgttga atatggctca taacagaact tattatttcc
     7441 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
     7501 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
     7561 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc
     7621 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg
     7681 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa
     7741 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta
     7801 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
     7861 atatattgtg gtgtaaacat aacggatccg tctcaggaga gcg
//
