LOCUS       sequence-405330-       10036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conditional expression of target gene Dach1 with fluorescent 
            reporter mScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10036)
  AUTHORS   Fisher J, Verhagen M, Long Z, Moissidis M, Yan Y, He C, Wang J, 
            Micoli E, Alastruey CM, Moors R, Marin O, Mi D, Lim L
  TITLE     Cortical somatostatin long-range projection neurons and interneurons
            exhibit divergent developmental trajectories.
  JOURNAL   Neuron. 2023 Dec 7:S0896-6273(23)00887-5. doi: 
            10.1016/j.neuron.2023.11.013.
  PUBMED    38086373
REFERENCE   2  (bases 1 to 10036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2023 Dec 7:S0896-6273(23)00887-5. doi: 
            10.1016/j.neuron.2023.11.013."
COMMENT     Sequence Label: p254-RV-CAG-dio-mScarlet-T2A-Dach1-202
FEATURES             Location/Qualifiers
     source          1..10036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        135..514
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        515..718
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     669..689
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             719..894
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    957..1156
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1357..1773
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1703..1725
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1741..1757
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1787..2166
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2168..2444
                     /label=chicken beta-actin promoter
     intron          2445..3461
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3385..3407
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3469..3488
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(3531..3564)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(3615..3648)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(5919..5972)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5973..6668)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(6248..6267)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6517..6535
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    6743..6776
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    6827..6860
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6870..7458
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6923..6943)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7341..7352)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7641..8110
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8240..8257)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8411..8999)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8491..8510)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9159..9950,9951..10019))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9782..9801
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gatccggcca ttagccatat tattcattgg ttatatagca taaatcaata ttggctattg
       61 gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac
      121 attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      181 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      241 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      301 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      361 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      421 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      481 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      541 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      601 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      661 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctca
      721 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
      781 ggtacccgta ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg tctcgctgtt
      841 ccttgggagg gtctcctctg agtgattgac tacccacgac gggggtcttt catttggggg
      901 ctcgtccggg atttggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
      961 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgtttga tgttatgcgc
     1021 ctgcgtctgt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
     1081 gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt
     1141 tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt
     1201 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     1261 tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc
     1321 tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta
     1381 agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat
     1441 gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga
     1501 tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt
     1561 tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg
     1621 gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt
     1681 cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc
     1741 ctttatccag ccctcactcc ttctctaggc gccggaattc gatgtcgaca ttgattattg
     1801 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     1861 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     1921 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     1981 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2041 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2101 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2161 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
     2221 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
     2281 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
     2341 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
     2401 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
     2461 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
     2521 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
     2581 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
     2641 gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
     2701 ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg
     2761 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
     2821 gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
     2881 cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg
     2941 ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg
     3001 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg
     3061 gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
     3121 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     3181 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     3241 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
     3301 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
     3361 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     3421 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     3481 ttattgtgct gtctcatcat tttggcaaag aattcgctag cggatccgga ataacttcgt
     3541 ataggatact ttatacgaag ttatgcagaa tggtagctgg attgtagctg ctattagcaa
     3601 tatgaaacct cttaataact tcgtatagca tacattatac gaagttattt aattaatcag
     3661 tacatgacag tagttttcaa atacagtctt ccatcttgta ttgtcctttc agcatctgct
     3721 ctccctccgc tgcggtcagc ttctatctca ggggtcaggg agtcatttaa gacccggaga
     3781 ctgtccgctg aagctgcctg tttcagtgtc tgctccgctt gctcacggcg ttttgtctca
     3841 aattctagtg cctcctgcag ttttctcttt gctttctttt ccttctttag cctcttttga
     3901 actatggctc tgttcttttg ttccatggcc agctgcttct ccagtgtttc tcttagttct
     3961 ctttctctta aaaaatccat cttcagctct gttttttcca gttggacctg cttttcttga
     4021 gctctggcat tgtctatggc aactttcaag aggccctgta tgttagtgag aagagtctct
     4081 atggaggaca ggccatcagg aaacagaaag ggagatggga agccgggagg tagtggttgt
     4141 ccatgcccag ttagagaaag tttgtcgatg ctgtctcttg cggttggtgt ggaaagtggg
     4201 gtctcatctt tttcaatgtg gatccgtttt gactcatgcc ccatgtcatg accagccaaa
     4261 tccccctcct ttggcccagg aagcacattt ggggataaac ccataagcat ctggttcatg
     4321 gacaggccat tctgatggac agggattctg tcggaagaac tctcagtccg cgccggggag
     4381 ctggacacac tgctgctgcg gtgtgaggat gggtggctgc cgggcctccg gccctcctcc
     4441 agagatggag caggcgaggg actgtcggga acacgctcct taataacaga tgtctccacc
     4501 ctggatggag gactctgaac ttgtgctgcc gccgccatat ttgcaatggt gctaaggtgg
     4561 ttcatctgac tcattgccat ggtgacagat gctggaggta ggctgacagg aatgagaggg
     4621 tggggcatca tcataaaagg aagttccagt ccatttggcg gaaggtggct gtgctgcaga
     4681 tggttgagag gatggctaac tggaaggcta aacgctgcag gcatgcgggc atgtgcaaca
     4741 ggagcctggg atccctggga tgggggagag tgcagtgttt ccttatccca agaaccacca
     4801 ctgctgccaa cacttgaatt catgtccccg ttttcagaat ctgctccatg ttggttgtta
     4861 ctggcatgat agttgctcat agcttctaat tttatttttt tcaccttcat tgcttcagca
     4921 atagctgcat tggtagcagc tgcagcagct gcagcagctg cagtcagacc tgttggtgga
     4981 atgattccag gagacatgag gccagggaca gaatgcggca tgatgtgaga gttctctggg
     5041 gaagtgacac tttgagtcct cttaggaggc cttccaggtc tggaactggc gttggtgcag
     5101 tcattgtaga gggtctcgaa gtccttcctg gagatgagtt tgcagcggtt cactccgggc
     5161 tggatggccc ccagtcccct caggatgcga acctgttcca cattgcacac caccggcgtg
     5221 atctccaacc gcttcagctt ggtgtagacg gtgtgcaagc cccccaccaa gtgcttcagg
     5281 aacaggtcga aagcctgggg caggcagatc agctcgcagc cctccaccgt aaaggaagcc
     5341 actttggccc ctctcagatc caccattttg cactcattat tctggggggt gttttccact
     5401 ggggacgggg ttgagtacac gggtttccca gggagggggc cgcagctgct gctgctactg
     5461 ctgctgctgc tgctactgct gctgctgctg ctactgctgc tgctgctgct gctactgctg
     5521 ctgctgccgg tgctcgcggt gatgggggtg ctggaggcgc cgccgccgcc agcgctaacg
     5581 ccgccgccgc tgctcccggc cgccaggctg gggttgcagt tgctcccgcc gccgcctccg
     5641 ttgccgccgc tgccaccgcc gcctccgctg ccgccgccgc cgccaccagg agaggtgact
     5701 gtggccgcgg ctgaagaaga agcagaagag gcaatgggct ccggccggaa cagagttggc
     5761 ccagacgacg ccgggggtcc gatggatgga gccggagacg aggtcgccga ggaggtggag
     5821 gtggtggtgc ccgaggagga agcagaagta gagatcgggg gttgaggggg gaccagctgg
     5881 gtcggaggga tcaaagccgc cggcactgcc atctcgagag ggccgggatt ctcttccacg
     5941 tctccgcatg tcagaagact tcctctgccc tccttgtaca gctcgtccat gccgccggtg
     6001 gagtggcggc cctcggagcg ttcgtactgt tccaccacgg tgtagtcctc gttgtgggag
     6061 gtgatgtcca acttgcggtc gacgttgtag gcgccgggca tctgcacggg cttcttggcc
     6121 ttgtaggtgg tcttgaagtc cgccaggtag cggccgccgt ccttcaggcg cagggccatc
     6181 ttaatgtcgc ccttcagcac gccgtcctcg gggtacaacc gctcggtgga cgcttcccag
     6241 cccattgtct tcttctgcat tacggggccg tcaggaggga agttggtgcc gcggagcttc
     6301 accttgtaga tcagggtgcc gtcctccagg gaggtgtcct gggtcacggt cacggcgccg
     6361 ccgtcctcga agttcatcac gcgctcccac ttgaagccct cggggaagga ctgcttatag
     6421 tagtcgggga tgtcggcggg gtgcttgatg aaggccctgg agccgtacat gaactgaggg
     6481 gacaggatgt cccaggagaa gggcaggggg ccacccttgg tcaccttcag cttggcggtc
     6541 tgggtgccct cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc
     6601 atggagccct ccatgtgcac cttgaaccgc atgaactcct tgatcactgc ctcgcccttg
     6661 ctcaccatga tcttttggtc ctcatcattc ttttcaacct gtttggttct tctcatacag
     6721 cacagcatgg tggcgcgccg cgataacttc gtataaagta tcctatacga agttatttgc
     6781 cttaacccag aaattatcac tgttattctt tagaatggtg caaagaataa cttcgtataa
     6841 tgtatgctat acgaagttat gtttaaacca atcaacctct ggattacaaa atttgtgaaa
     6901 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6961 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     7021 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     7081 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7141 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7201 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7261 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7321 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7381 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7441 tttgggccgc ctccccgcct ggaattcgag ctcgacgata aaataaaaga ttttatttag
     7501 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     7561 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag
     7621 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     7681 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
     7741 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     7801 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     7861 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     7921 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
     7981 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     8041 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     8101 gggtctttca catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca
     8161 ttaaatggcc atagttgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8221 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8281 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8341 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8401 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8461 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8521 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8581 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8641 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8701 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8761 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8821 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8881 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8941 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9001 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9061 ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg cggccgcaaa
     9121 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     9181 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9241 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9301 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9361 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9421 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9481 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9541 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9601 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9661 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9721 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9781 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9841 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9901 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9961 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    10021 ctcttccttt ttcaat
//
