LOCUS       sequence-405331-        9081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conditional expression of target gene Brn2 with fluorescent reporter
            mScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Fisher J, Verhagen M, Long Z, Moissidis M, Yan Y, He C, Wang J, 
            Micoli E, Alastruey CM, Moors R, Marin O, Mi D, Lim L
  TITLE     Cortical somatostatin long-range projection neurons and interneurons
            exhibit divergent developmental trajectories.
  JOURNAL   Neuron. 2023 Dec 7:S0896-6273(23)00887-5. doi: 
            10.1016/j.neuron.2023.11.013.
  PUBMED    38086373
REFERENCE   2  (bases 1 to 9081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2023 Dec 7:S0896-6273(23)00887-5. doi: 
            10.1016/j.neuron.2023.11.013."
COMMENT     Sequence Label: p194-RV-CAG-dio-mScarlet-T2A-Brn2
FEATURES             Location/Qualifiers
     source          1..9081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     4..24
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             54..229
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    292..491
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             692..1108
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1038..1060
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1076..1092
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1122..1501
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1503..1779
                     /label=chicken beta-actin promoter
     primer_bind     2689..2711
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2773..2792
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(2835..2868)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(2919..2952)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(4299..4352)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(4353..5048)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(4628..4647)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4897..4915
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    5123..5156
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    5207..5240
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5250..5838
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5303..5323)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5721..5732)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6021..6490
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6620..6637)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6791..7379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6871..6890)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7539..8330,8331..8399))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8162..8181
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     enhancer        8551..8930
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(8931..9081,1..53)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcaataaa
       61 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
      121 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      181 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      241 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      301 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      361 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      421 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      481 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      541 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      601 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
      661 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
      721 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
      781 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
      841 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
      901 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
      961 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1021 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1081 tccagccctc actccttctc taggcgccgg aattcgatgt cgacattgat tattgactag
     1141 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     1201 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     1261 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     1321 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     1381 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     1441 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1501 ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc
     1561 aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg
     1621 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
     1681 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
     1741 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
     1801 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
     1861 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
     1921 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
     1981 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     2041 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
     2101 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     2161 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
     2221 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
     2281 cacggcccgg cttcgggtgc ggggctccgt acggggggtg gcggcaggtg ggggtgccgg
     2341 gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga
     2401 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg
     2461 agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc
     2521 gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg
     2581 gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg
     2641 gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct
     2701 ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc
     2761 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcg
     2821 ctagcggatc cggaataact tcgtatagga tactttatac gaagttatgc agaatggtag
     2881 ctggattgta gctgctatta gcaatatgaa acctcttaat aacttcgtat agcatacatt
     2941 atacgaagtt atttaattaa tcactggacg ggcgtctgca ccccgtggtg tggtggagtg
     3001 tccctactcc ccccgtacac atcctcggcg cccggcagag tccctccggg aggggtcatc
     3061 cttttctctt tctgtctcct gttacaaaac caaactctca ccacctcctt ctccagctgt
     3121 aagctgtccg cgagggaggt gatctcctgg gccgagggct tggggcattt gaggaaatgg
     3181 ctctccagag cccccttgac gctcacctcg atggaggtcc gctttttccg cttgcgccct
     3241 tgcgctgcga tcttgtctat gctcgtgggg ctgcccgagg acgagtccgc ctcctccaac
     3301 cacttgttca acaaaggctt cagcttgcac atgttcttga agctcagctg cagggcctca
     3361 aacctgcaga tggtggtctg cgagaacacg ttgccataca gggtgcccag agccagcccc
     3421 acgtccgctt gggtaaatcc cagtttgatc cgccgctgct tgaactgctt ggcgaactgc
     3481 tccaggtcgt ccgaggtcgg cgtgtcctcg tccgagtgcg ggtcgtggtg cgcgcctggg
     3541 tggccaggcg gaccctgcgg gggcggcggg ggcggcggct gctggtgtgg gtgcgagtgc
     3601 gggtgcgggt ggtggtcggc atggtgtggc tcgtcgtgcg cgtcccgcag gccgtggtgg
     3661 tgcagaccgg ccggctgccc gccggcgccc agcatgccgt tcaccgtgaa gctgggctgc
     3721 gagtagagca agccgccgtt ggacgctccc atggagggtg ggaggtgcgc tgcagccgcc
     3781 gcgctccgcc atgccccggg tcccgggtgg tggttagcgg cgtggtgcac cagatgcggc
     3841 ggccgctgtt gctgctgctg ctgctgctgt tgctgctgtt gctgctgctg ttgctgttgc
     3901 tgctgctgat gctgctgctg cagggcgcct ggcccgtgca gctcgtctcc gcggccgccc
     3961 tgctgcacca ccaccgaggg cttgatgtcc ggctggccca gggggctggt ggaccacggg
     4021 gagccgtcgc cgccgccccc gccgccgccc ccgccccccc cgccgccgcc accgcccccg
     4081 ccgccgccgc cgtgggacag cgcggtgatc cactggtgag cgtggctgag cgggtgtccg
     4141 ttgctctgca gagcgccgta gtcgccctgc accaggctct gcgcttcgcg gtagcccccc
     4201 gcgccctgct gcatgccgcc gggcggctcg gcgtgcacga tggaggcgct ggaggtgagc
     4261 aggctgtagt ggttagacgc tgcggtcgcc atctcgagag ggccgggatt ctcttccacg
     4321 tctccgcatg tcagaagact tcctctgccc tccttgtaca gctcgtccat gccgccggtg
     4381 gagtggcggc cctcggagcg ttcgtactgt tccaccacgg tgtagtcctc gttgtgggag
     4441 gtgatgtcca acttgcggtc gacgttgtag gcgccgggca tctgcacggg cttcttggcc
     4501 ttgtaggtgg tcttgaagtc cgccaggtag cggccgccgt ccttcaggcg cagggccatc
     4561 ttaatgtcgc ccttcagcac gccgtcctcg gggtacaacc gctcggtgga cgcttcccag
     4621 cccattgtct tcttctgcat tacggggccg tcaggaggga agttggtgcc gcggagcttc
     4681 accttgtaga tcagggtgcc gtcctccagg gaggtgtcct gggtcacggt cacggcgccg
     4741 ccgtcctcga agttcatcac gcgctcccac ttgaagccct cggggaagga ctgcttatag
     4801 tagtcgggga tgtcggcggg gtgcttgatg aaggccctgg agccgtacat gaactgaggg
     4861 gacaggatgt cccaggagaa gggcaggggg ccacccttgg tcaccttcag cttggcggtc
     4921 tgggtgccct cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc
     4981 atggagccct ccatgtgcac cttgaaccgc atgaactcct tgatcactgc ctcgcccttg
     5041 ctcaccatga tcttttggtc ctcatcattc ttttcaacct gtttggttct tctcatacag
     5101 cacagcatgg tggcgcgccg cgataacttc gtataaagta tcctatacga agttatttgc
     5161 cttaacccag aaattatcac tgttattctt tagaatggtg caaagaataa cttcgtataa
     5221 tgtatgctat acgaagttat gtttaaacca atcaacctct ggattacaaa atttgtgaaa
     5281 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5341 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5401 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5461 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     5521 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5581 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     5641 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5701 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5761 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5821 tttgggccgc ctccccgcct ggaattcgag ctcgacgata aaataaaaga ttttatttag
     5881 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     5941 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag
     6001 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     6061 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
     6121 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     6181 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     6241 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     6301 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
     6361 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     6421 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     6481 gggtctttca catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca
     6541 ttaaatggcc atagttgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6601 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6661 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6721 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6781 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6841 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6901 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6961 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7021 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7081 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7141 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7201 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7261 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7321 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7381 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7441 ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg cggccgcaaa
     7501 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7561 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7621 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7681 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7741 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7801 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7861 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7921 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7981 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     8041 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     8101 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     8161 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     8221 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     8281 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     8341 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8401 ctcttccttt ttcaatgatc cggccattag ccatattatt cattggttat atagcataaa
     8461 tcaatattgg ctattggcca ttgcatacgt tgtatccata tcataatatg tacatttata
     8521 ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt tattaatagt
     8581 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     8641 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     8701 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     8761 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     8821 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     8881 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     8941 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     9001 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     9061 gtcgtaacaa ctccgcccca t
//
