LOCUS       sequence-405332-       12813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox inducible FLAG tag ARID1a in a sleeping beauty transposon 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12813)
  AUTHORS   Yang Y, Gomez N, Infarinato N, Adam RC, Sribour M, Baek I, Laurin M,
            Fuchs E
  TITLE     The pioneer factor SOX9 competes for epigenetic factors to switch 
            stem cell fates.
  JOURNAL   Nat Cell Biol. 2023 Jul 24. doi: 10.1038/s41556-023-01184-y.
  PUBMED    37488435
REFERENCE   2  (bases 1 to 12813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2023 Jul 24. doi: 10.1038/s41556-023-01184-y."
COMMENT     Sequence Label: pT5_TRE-ARID1A-FLAG_PGK-H2B-RFP
FEATURES             Location/Qualifiers
     source          1..12813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     69..91
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     83..100
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     84..100
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     110..129
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        110..128
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    442..460
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    484..502
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    526..544
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    568..586
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    610..628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    652..670
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        703..741
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     738..762
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6572..6581
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7703..7726
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    7771..7852
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7978..8488
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8407..8425
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8500..8877
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      8890..8899
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8896..9573
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             8896..9573
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(9026..9044)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9294..9313
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9488..9511
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    9633..9714
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10109..10129)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10109..10127)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10148..10164)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10148..10164)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10161..10183)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10172..10188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10196..10202,10203..10220,10221..10226))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10241..10262
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10379..10396)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10550..11138)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10630..10649)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11309..12100,12101..12169))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAAKLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11932..11951
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12170..12274)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12301..12756
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12388..12407)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12598..12619
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
       61 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc
      121 actatagggc gaattggagc tagcgtttaa acctacagtt gaagtcggaa gtttacatac
      181 acttaagttg gagtcattaa aactcgtttt tcaactactc cacaaatttc ttgttaacaa
      241 acaatagttt tggcaagtca gttaggacat ctactttgtg catgacacaa gtcatttttc
      301 caacaattgt ttacagacag attatttcac ttataattca ctgtatcaca attccagtgg
      361 gtcagaagtg tacatacacg cgcttgactg tgcctttaag cttgatatcg atgccctttc
      421 gtcttcactc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
      481 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
      541 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
      601 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
      661 gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg
      721 gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
      781 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcga ccggtccgca
      841 ggtatgcacg cgtatggccg cgcaggtcgc ccccgccgcc gccagcagcc tgggcaaccc
      901 gccgccgccg ccctcggagc tgaagaaagc cgagcagcaa cagcgggagg aggcgggggg
      961 cgaggcggcg gcggcagcgg ccgagcgcgg ggaaatgaag gcagccgccg ggcaggagag
     1021 cgagggcccc gccgtggggc cgccgcagcc gctgggaaag gagctgcagg acggggccga
     1081 gagcaatggg ggtggcggcg gcggcggagc cggcagcggc ggcgggcccg gcgcggagcc
     1141 ggacctgaag aactcgaacg ggaacgcggg ccctaggccc gccctgaaca ataacctccc
     1201 ggagccgccc ggcggcggcg gcggcggcgg cagcagcagc agcgacgggg tgggggcgcc
     1261 tcctcactcg gccgcggccg ccctgccgcc cccagcctac ggcttcgggc aagcctacgg
     1321 ccggagcccg tctgccgtcg ccgccgcggc ggccgccgtc ttccaccaac aacatggcgg
     1381 acaacaaagc cctggcctgg cagcgctgca gagcggcggc ggcgggggct tggagcccta
     1441 cgccgggccc cagcagaact cgcacgacca cggcttcccc aaccaccagt acaactccta
     1501 ctaccccaac cgcagcgcct accccccgcc tccccaggcc tacgcgctga gctccccgag
     1561 aggtggcact ccgggctccg gcgcggcggc ggccgccggc tccaagccgc ctccctcctc
     1621 cagcgcctct gcctcctcgt cgtcttcgtc cttcgcacag cagcgcttcg gggccatggg
     1681 gggaggcggc ccctcagcgg ccggcggggg aactccccag cccaccgcca cccccaccct
     1741 caaccaactg ctcacgtcgc ccagctcggc ccgtggctac cagggctacc ccgggggcga
     1801 ctacggcggc gggccccagg acgggggcgc gggcaaaggc ccggcggaca tggcctcgca
     1861 gtgctggggg gctgcggcgg cggcggcggc ggcggcagcg gccgtctcgg gaggggccca
     1921 acaaaggagc caccacgcgc ccatgagccc cgggagcagc ggcggcgggg ggcagccgct
     1981 cgcccggacc cctcagtcat ccagtccaat ggatcagatg ggaaagatga gacctcagcc
     2041 gtatggtggg actaacccat actcgcaaca acagggacct ccttcaggac cgcaacaagg
     2101 acatgggtac ccagggcagc catatgggtc ccagactcca cagcggtacc ccatgaccat
     2161 gcagggccgg gctcagagtg ccatgggcag cctctcttat gcacagcaga ttccacctta
     2221 tggccagcaa ggccccagtg cgtatggcca gcagggccag actccatact ataaccagca
     2281 aagtcctcat ccccagcagc agccacctta cgcccagcaa ccaccatccc agacccctca
     2341 tgcccagcct tcgtatcagc agcagccgca gactcagcaa ccacagcttc agtcctctca
     2401 gcctccatat tcccagcagc catcccagcc tccacatcag cagtccccaa ctccatatcc
     2461 ctcccagcag tccaccacac aacagcatcc ccagagccag cccccctact cacaaccaca
     2521 ggcacagtct ccctaccagc agcagcaacc tcagcagcca gcatcctcgt cgctctccca
     2581 gcaggctgca tatcctcagc cccagcctca gcagtcccag caaactgcct attcccagca
     2641 gcgcttccct ccaccacagg agctttctca agattcattt gggtctcagg catcctcagc
     2701 cccctcaatg acctccagta agggagggca agaagatatg aacctgagtc ttcagtcaag
     2761 gccctccagc ttgcctgatc tgtctggttc aatcgatgat ctccccatgg ggacagaagg
     2821 agctctgagt cctggcgtga gcacatcagg gatttccagc agccaaggag agcagagcaa
     2881 tccagctcag tctccctttt ctcctcacac ctcccctcac ctgcctggca tccgaggccc
     2941 gtccccgtcc cctgttggct ctcctgccag tgtcgcgcag tctcgctcag gaccactctc
     3001 gcctgctgca gtgccaggca accagatgcc acctcggcca cccagtggcc agtcagacag
     3061 catcatgcac ccttccatga accaatcaag cattgcccaa gatcgaggtt atatgcagag
     3121 gaacccccag atgccccagt acacttcccc tcagcctggc tcggccttat ccccacgtca
     3181 gccgtctgga ggacagatgc actcgggcgt gggctcctac cagcagaact ccatggggag
     3241 ctacggcccc cagggcagtc agtatggccc acaaggaggc tatcctaggc agcctaacta
     3301 taatgccttg cccaacgcca actaccccaa tgcaggcatg gccggaagta tgaaccctat
     3361 gggtgctgga ggtcagatgc atgggcagcc tggaatccca ccttacggca cactccctcc
     3421 agggagaatg gctcatgcgt ctatgggcaa caggccctat ggccctaata tggccaatat
     3481 gccacctcag gttgggtcag ggatgtgtcc tccaccaggg ggaatgaaca ggaaaactca
     3541 agagtctgct gttgccatgc atgttgctgc caactctatc caaaacaggc caccaggcta
     3601 cccaaatatg aatcaagggg gcatgatggg aactggacct ccctatggac aggggatcaa
     3661 tagtatggct ggcatgatca accctcaggg acccccatat cctatgggtg gaaccatggc
     3721 caacaattca gcagggatgg cagccagccc agagatgatg ggccttgggg atgttaagtt
     3781 aactcccgcc acaaaaatga acaacaaggc agatggaaca cccaagacag aatccaaatc
     3841 taagaaatcc agttcttcta ccaccaccaa tgagaagatc accaaattgt atgagttggg
     3901 tggtgagccc gagaggaaga tgtgggtgga ccggtacctg gccttcacag aggagaaggc
     3961 catgggcatg acaaatctgc ctgctgtggg gaggaagcct ctggacctct atcgcctcta
     4021 tgtgtctgtg aaggagattg gtgggttgac tcaggtcaac aagaacaaaa aatggcggga
     4081 acttgcaacc aacctcaatg tgggtacatc aagcagtgct gccagctcac tgaaaaagca
     4141 gtatatccaa tgtctctatg cctttgagtg caagatcgag cgtggagaag accctccccc
     4201 cgatatcttc gcagctgctg actccaagaa gtcccaaccc aagatccagc ccccctctcc
     4261 tgcgggatca gggtctatgc aggggccaca aactcctcag tcaaccagca gttctatggc
     4321 agaaggagga gacctgaagc caccaactcc agcatccaca ccacatagtc aaattccccc
     4381 cttaccaggc atgagcagga gcaactcagt cggaatccag gatgcctttc ctgatggaag
     4441 tgaccccaca ttccagaagc ggaattccat gactccaaac cctgggtacc agcccagtat
     4501 gaatacctct gacatgatgg ggcgcatgtc ctatgagcca aataaggatc cttatggcag
     4561 catgaggaaa gcgccaggaa gtgatccctt catgtcctca gggcagggcc ccaatggcgg
     4621 gatgggtgat ccctacagcc gtgctgctgg ccctgggctg ggaagtgtgg cgatgggacc
     4681 acggcagcac tatccctatg gaggtcctta cgacagagtg aggacggagc ctggaatcgg
     4741 gcctgaagga aatatgggca ctggagcccc tcagccaaat ctcatgcctt ccaccccaga
     4801 ttcggggatg tattctccta gccgctaccc cccgcagcag cagcagcaac agcagcaaca
     4861 acatgattcc tatggcaatc aattctctac ccaaggcacc ccttccagca gccccttccc
     4921 cagccagcag accacaatgt atcagcagca gcagcagaat tataagaggc caatggatgg
     4981 cacatatggc ccccctgcca agcggcatga aggggagatg tacagtgtgc cgtacagcgc
     5041 tgggcaaggc cagcctcaac agcagcagtt gcctgcagct cagtcccagc ctgccagcca
     5101 gccacaagct gcccagcctt cccctcagca ggacgtgtac aaccagtaca gcaatgccta
     5161 ccctgcctcc gccaccgctg ctactgatcg ccgaccagca ggcggccccc agaaccaatt
     5221 tccattccag tttggccgag accgagtctc tgcacctcct ggttccagtg cccagcagaa
     5281 catgccacca caaatgatgg gtggccccat acaggcatca gctgaggttg ctcagcaggg
     5341 caccatgtgg caggggcgaa atgacatgac ctacaattat gccaacaggc agaacacagg
     5401 ctctgccacc cagggccctg cgtatcatgg tgtgaaccga acagatgaaa tgctccacac
     5461 agatcagagg gccaaccatg aaggcccatg gccttcccat ggcacacgcc agcctccgta
     5521 tggtccttca gcccctgttc cccccatgac aaggccccct ccatctaact accagccccc
     5581 accaagcatg ccgaatcaca ttcctcaggt atccagcccc gctcccctcc cccggcccat
     5641 ggagaaccgt acttctccta gcaagtctcc attcctgcac tctgggatga aaatgcaaaa
     5701 ggcgggtcca ccggtgcctg cttcgcacat agcgcctacc cctgtgcagc cgcctatgat
     5761 tcggcgggat atcaccttcc cacctggctc tgtagaggcc actcagcctg tgttgaagca
     5821 gagaaggcgg ctcacaatga aagacattgg aaccccggag gcatggcggg taatgatgtc
     5881 cctcaagtcc gggctcctgg cagagagcac gtgggcgtta gacaccatta acattctact
     5941 gtatgatgac aacagcatta tgaccttcaa cctcagccag ctcccaggct tgctagagct
     6001 ccttgtggaa tatttccgta gatgcctaat tgaaatcttt ggcattttaa aggagtatga
     6061 ggtaggggac ccaggacaga gaacattact agaccctggg agattcacca aggtgtatag
     6121 tccagcccat acagaggaag aagaggaaga acaccttgat cctaaactgg aggaggaaga
     6181 ggaagaaggg gttggaaatg atgaggagat ggcctttttg ggcaaggaca agccatcttc
     6241 agagaataat gaggagaagc tagtcagtaa gtttgacaag cttccggtaa agatcgtgca
     6301 gaggaatgac ccatttgtgg tggactgctc agataagctt gggcgcgtgc aggagtttga
     6361 cagtggcctg ctacactggc ggattggtgg tggggatacc actgagcata tccagaccca
     6421 ctttgagagc aagatagagc tgctgccttc ccggccttat gtgccctgcc caacgccccc
     6481 tcggaaacac ctcacaacag tagagggcac accagggaca acggagcagg agggcccccc
     6541 gcccgatggc cttccagaga aaaggatcac agccaccatg gatgacatgt tgtctacccg
     6601 gtctagcaca ttgactgatg agggggcaaa gagtgcagag gccaccaagg aaagcagcaa
     6661 gtttccattt ggcattagcc cagcacagag ccaccggaac atcaaaattt tagaggatga
     6721 accccatagt aaggatgaga ccccactgtg tacccttctg gactggcagg attcccttgc
     6781 taagcgctgt gtctgtgtct ccaataccat ccggagcctg tcgtttgtgc caggcaacga
     6841 ctttgagatg tccaaacacc cagggctgct gcttatcctg ggcaagctga tcctgctgca
     6901 ccacaagcac ccagagcgga agcaggcacc actaacttat gagaaggagg aggaacagga
     6961 ccaaggggtg agctgtgaca aagtggagtg gtggtgggac tgcttggaga tgctccgaga
     7021 aaacacgctg gtcaccctcg ccaacatctc ggggcaattg gacctatccc catatcctga
     7081 gagcatctgc ctgcctgtcc tggacggact cctacactgg gcagtttgcc cttcagctga
     7141 agcccaggac cccttctcaa ccctaggccc caatgccgtc ctctcccccc agagattggt
     7201 cttggaaacc ctcagcaaac tcagcatcca ggacaacaat gtggacctga tcctggccac
     7261 tccccctttt agccgcctgg agaagttgta tagtaccatg gtgcgcttcc tcagtgaccg
     7321 aaagaaccca gtgtgccggg agatggccgt ggtactgctg gcaaatctgg cccaggggga
     7381 cagcctggca gcccgggcca ttgcagtgca gaagggcagc atcggcaacc tcctgggttt
     7441 cctggaggac agccttgctg ccacacagtt ccagcagagc caggcaagcc tcctgcatat
     7501 gcagaatcca ccctttgaac caactagtgt ggacatgatg cggcgggctg cccgagcact
     7561 gcttgccctg gccaaggtgg atgagaacca ctcagagttc actctgtatg agtcacggct
     7621 gttggacatc tccgtgtcac cactgatgaa ctcattggtt tcacaagtca tttgtgatgt
     7681 actgtttttg attggccagt cagattacaa ggatgacgac gataaggttt aagcatgcct
     7741 agatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     7801 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     7861 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     7921 gtaaaacctc tacaaatgtg gtacaatgca tctagagggg gtaccccgcg gctcgagggg
     7981 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
     8041 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
     8101 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
     8161 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
     8221 agccgcacgt ctcactagta ccctcgcaga cggacagcac cagggagcaa tggcagcgcg
     8281 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
     8341 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
     8401 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
     8461 tgaccgaatc accgacctct ctccccaggg ggatctacca tgccagagcc agcgaagtct
     8521 gctcccgccc cgaaaaaggg ctccaagaag gcggtgacta aggcgcagaa gaaaggcggc
     8581 aagaagcgca agcgcagccg caaggagagc tattccatct atgtgtacaa ggttctgaag
     8641 caggtccacc ctgacaccgg catttcgtcc aaggccatgg gcatcatgaa ctcgtttgtg
     8701 aacgacattt tcgagcgcat cgcaggtgag gcttcccgcc tggcgcatta caacaagcgc
     8761 tcgaccatca cctccaggga gatccagacg gccgtgcgcc tgctgctgcc tggggagttg
     8821 gccaagcacg ccgtgtccga gggtactaag gccatcacca agtacaccag cgctaaggat
     8881 ccaccggtcg ccaccatggc ctcctccgag gacgtcatca aggagttcat gcgcttcaag
     8941 gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     9001 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
     9061 ttcgcctggg acatcctgtc ccctcagttc cagtacggct ccaaggccta cgtgaagcac
     9121 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     9181 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     9241 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     9301 atgcagaaga agaccatggg ctgggaggcc tccaccgagc ggatgtaccc cgaggacggc
     9361 gccctgaagg gcgagatcaa gatgaggctg aagctgaagg acggcggcca ctacgacgcc
     9421 gaggtcaaga ccacctacat ggccaagaag cccgtgcagc tgcccggcgc ctacaagacc
     9481 gacatcaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     9541 cgcgccgagg gccgccactc caccggcgcc taactcgaga ctagtcgacg tgaggatcct
     9601 ctagatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     9661 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     9721 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     9781 aagtaaaacc tctacaaatg tggtacagat ctttaaacaa tttaaaggca atgctaccaa
     9841 atactaagcg cgtgtatgta cacttctgac ccactgggaa tgtgatgaaa gaaataaaag
     9901 ctgaaatgaa tcattctctc tactattatt ctgatatttc acattcttaa aataaagtgg
     9961 tgatcctaac tgaccttaag acagggaatc tttactcgga ttaaatgtca ggaattgtga
    10021 aaaagtgagt ttaaatgtat ttggctaagg tgtatgtaaa cttccgactt caactgtagt
    10081 ttaaacgggc ccggtccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
    10141 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    10201 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    10261 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    10321 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    10381 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    10441 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    10501 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    10561 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    10621 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    10681 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaacc gtggcgcttt
    10741 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    10801 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    10861 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    10921 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    10981 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    11041 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    11101 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    11161 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    11221 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    11281 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    11341 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    11401 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    11461 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    11521 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    11581 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    11641 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    11701 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    11761 gaagtaactt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    11821 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    11881 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    11941 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    12001 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    12061 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    12121 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    12181 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    12241 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    12301 acgcgccctg tagcggcgca ttaagcgcgg cgggagtggt ggttacgcgc agcgtgaccg
    12361 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
    12421 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
    12481 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
    12541 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
    12601 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
    12661 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
    12721 acgcgaattt taacaaaata ttaacgctta caatttccat tcgccattca ggctgcgcaa
    12781 ctgttgggaa gggcgatcgg tgcgggcctc ttc
//
