LOCUS       sequence-405379-        2307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the tetracycline resistance gene insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo9
FEATURES             Location/Qualifiers
     source          1..2307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             258..1448
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYASSASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(610..629)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     terminator      complement(1468..1499)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     terminator      complement(1623..1659)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     oriT            1835..1944
                     /note="incP origin of transfer"
     rep_origin      join(2059..2307,1..140)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 aaaaaaaaga atatataagg cttttaaagc ttttaaggtt taacggttgt ggacaacaag
       61 ccagggatgt aacgcactga gaagccctta gagcctctca aagcaatttt gagtgacaca
      121 ggaacactta acggctgaca tgggggtctc gcgctccggt agtacgtaag aggttccaac
      181 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca
      241 ggagttaaga agctaaaatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca
      301 ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata
      361 tcgtccattc cgacagcatc gccagtcact atggcgtgct gctagcgcta tatgcgttga
      421 tgcaatttct atgcgcaccc gttctcggag cactgtccga ccgctttggc cgccgcccag
      481 tcctgctcgc ttcgctactt ggagccacta tcgactacgc gatcatggcg accacacccg
      541 tcctgtggat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
      601 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
      661 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
      721 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
      781 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaccg atgcccttga
      841 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
      901 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
      961 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
     1021 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
     1081 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
     1141 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca
     1201 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac
     1261 agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcattgga ccgctgatcg
     1321 tcacggcgat ttatgcctcc tcggcgagca catggaacgg gttggcatgg attgtaggcg
     1381 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct
     1441 cgacctgaat ggaagggatc cccctcaagt caaaagcctc cggtcggagg tttttgactt
     1501 tctgctatgg aggtcaggta tgatttaaat ggtcagtatt gagcgatatc tagagaattc
     1561 gtcaacgaat tcaagcttga tatcattcag gacgagcctc agactccagc gtaactggac
     1621 tgaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg ttccggagcg agaccgtcaa
     1681 ttcgaggggg atcaattccg ttttccgctg cataaccctg cttcggggtc attatagcga
     1741 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg
     1801 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac
     1861 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga
     1921 atcctgctct gcgaggctgg ccggctaccg ccggcgtaac agatgagggc aagcggatgg
     1981 ctgatgaaac caagccaacc aggaagggca gcccacctat cacggaattg atccggccac
     2041 gatgcgtccg gcgtagagga tctgaagatc agcagttcaa cctgttgata gtacgtacta
     2101 agctctcatg tttcacgtac taagctctca tgtttaacgt actaagctct catgtttaac
     2161 gaactaaacc ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg
     2221 tttcacgtac taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat
     2281 agcctctaag gttttaagtt ttataag
//
