LOCUS       sequence-405382-        6811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  thrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration 
            vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with
            multi cloning sites (MCS) for fast and interchangeable cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   Altinoglu I,  Carballido-Lopez R
  TITLE     New PALM-compatible integration vectors for use in the Gram-positive
            model bacterium Bacillus subtilis
  JOURNAL   bioRxiv 2023
REFERENCE   2  (bases 1 to 6811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023"
COMMENT     Sequence Label: pIA028
FEATURES             Location/Qualifiers
     source          1..6811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             794..1501
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein"
                     /label=PAmCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALKG
                     EVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(936..954)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1204..1223
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     rep_origin      complement(4982..5570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5062..5081)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5741..6532,6533..6601))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6364..6383
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6602..6706)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6774..6792
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aattcatgta aaagatgagg ttggttcatt ctcgaaaatt acatctgtgt tctcagagcg
       61 gggcgtgagc tttgaaaaaa tccttcagct gccaattaaa ggccatgatg agttagctga
      121 aatcgtaatt gtcacacatc atacatcaga agctgatttc agtgatatcc tgcaaaacct
      181 aaatgatttg gaagtcgttc aagaagtcaa aagcacatat cgtgtagaag ggaacggttg
      241 gagctaatgt ggaaaggact tatccatcaa tataaagaat ttttacctgt aacagatcaa
      301 acaccggcgc taactttaca tgaaggaaac acacctctta ttcacctgcc gaagctgtct
      361 gagcagctcg gaattgagct tcatgtcaaa acggaaggcg tcaatcctac gggatcattt
      421 aaagatcgcg gaatggttat ggctgtggca aaggcaaaag aagaaggcaa tgacacgatt
      481 atgtgcgcgt caacaggtaa cacttccgct gctgcggcag catatgcagc ccgtgctaac
      541 atgaaatgca ttgtcatcat cccgaacgga aaaattgcat ttggaaaact cgctcaagct
      601 gtcatgtacg gagccgagat tatcgcaatt gacggaaact ttgacgatgc gcttaaaatt
      661 gtccgttcca tctgtgagaa atcaccgatt gcccttgtca actcagtcaa cccttaccgc
      721 attgaaggcc aaaaaactgc tgccttcgga tcgctagcga attcgagctc ggtacgggga
      781 tcctgcggcc gccgtgagca agggcgagga ggataacatg gccatcatta aggagttcat
      841 gcgcttcaag gtgcacatgg aggggtccgt gaacggccac gtgttcgaga tcgagggcga
      901 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
      961 ccccctgccc ttcacctggg acatcctgtc ccctcaattc atgtacggct ccaatgccta
     1021 cgtgaagcac cccgccgaca tccccgacta ctttaagctg tccttccccg agggcttcaa
     1081 gtgggagcgc gtgatgaaat tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     1141 cctgcaggac ggtgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     1201 cggccccgta atgcagaaga agaccatggg ctgggaggcc ctctccgagc ggatgtaccc
     1261 cgaggacggc gccctgaagg gcgaggtcaa gccgagagtg aagctgaagg acggcggcca
     1321 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     1381 ctacaacgtc aaccgcaagt tggacatcac ctcacacaac gaggactaca ccatcgtgga
     1441 acagtacgaa cgtgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta
     1501 aaggcatgca agcttggctg taattcgata accctaaagt tatggaaata agacttagaa
     1561 gcaaacttaa gagtgtgttg atagtgcatt atcttaaaat tttgtataat aggaattgaa
     1621 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
     1681 aaatgcgtaa tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
     1741 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
     1801 agataactaa aattacaaac aaatcgttta acttctgtat ttatttatag atgtaatcac
     1861 ttcaggagtg attacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
     1921 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
     1981 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
     2041 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaacatt
     2101 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
     2161 aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
     2221 gaaagccatg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
     2281 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
     2341 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
     2401 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
     2461 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
     2521 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     2581 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg
     2641 aaagttacac gttactaaag ggaatgtaga taaattatta ggtatactac tgacagcttc
     2701 caaggagcta aagaggtccc tagcgcctac ggggaatttg tatcgaagtg tgcgaacagc
     2761 tgggggaagc gcctgatgtt ttagcgatcc cggtcggaaa tgctggaaac atcactgcgt
     2821 actggaaggg cttcaaggaa tatcatgaga aaaacggcac aggccttccg aaaatgcgcg
     2881 gctttgaagc tgagggtgcg gcggcaatcg tgcgcaatga agtgattgaa aatccggaaa
     2941 caatagcgac agccattcgt atcggaaacc cggcaagctg ggacaaagct gtaaaggcag
     3001 ccgaggaatc caatgggaaa attgacgaag tcactgatga tgaaatcctt cacgcatatc
     3061 agctgatcgc ccgtgtagaa ggcgtgtttg cagaaccagg ttcttgcgcg tctatcgcag
     3121 gagtgctgaa acaggtgaaa tccggagaaa ttccgaaagg cagcaaggtc gtagctgtgt
     3181 taacaggaaa cggactgaaa gatccgaaca cagcggtcga catttcagaa atcaagcctg
     3241 tcacattgcc gactgatgaa gacagcatcc ttgaatatgt aaaaggagcg gcccgtgtat
     3301 gaacgaagcc gacatgctgt tctctgtcac tgttcccgga agcacagcta acctaggccc
     3361 cggctttgat tcagtcggaa tggcgctcag cagatatttg aagctgaccg tctttgaaag
     3421 cgacaaatgg tcttttgagg ctgaaacaga aacagtcgcc ggaattaatt cgagctcctt
     3481 taacttcctc accagtagtt gtatcggtac cataagtaga agcagcaacc caagtagctt
     3541 taccagcatc cggttcaacc agcatagtaa gaatcttact ggacatcggc agttcttcga
     3601 acagtgcgcc aactaccagc tctttctgca gttcattcag ggcaccggag aacctgcgtg
     3661 caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat ccatggatta
     3721 cgcgttaacc cgggcccgcg gatgcatatg atcagatctt aaggcctagg tctagaggat
     3781 cgatctgtat aataaagaat aattattaat ctgtagacaa attgtgaaag gatgtactta
     3841 aacgctaacg gtcagcttta ttgaacagta atttaagtat atgtccaatc tagggtaagt
     3901 aaattgagta tcaatataaa ctttatatga acataatcaa cgaggtgaaa tcatgagcaa
     3961 tttgattaac ggaaaaatac caaatcaagc gattcaaaca ttaaaaatcg taaaagattt
     4021 atttggaagt tcaatagttg gagtatatct atttggttca gcagtaaatg gtggtttacg
     4081 cattaacagc gatgtagatg ttctagtcgt cgtgaatcat agtttacctc aattaactcg
     4141 aaaaaaacta acagaaagac taatgactat atcaggaaag attggaaata cggattctgt
     4201 tagaccactt gaagttacgg ttataaatag gagtgaagtt gtcccttggc aatatcctcc
     4261 aaaaagagaa tttatatacg gtgagtggct caggggtgaa tttgagaatg gacaaattca
     4321 ggaaccaagc tatgatcctg atttggctat tgttttagca caagcaagaa agaatagtat
     4381 ttctctattt ggtcctgatt cttcaagtat acttgtctcc gtacctttga cagatattcg
     4441 aagagcaatt aaggattctt tgccagaact aattgagggg ataaaaggtg atgagcgtaa
     4501 tgtaatttta accctagctc gaatgtggca aacagtgact actggtgaaa ttacctcgaa
     4561 agatgtcgct gcagaatggg ctatacctct tttacctaaa gagcatgtaa ctttactgga
     4621 tatagctaga aaaggctatc ggggagagtg tgatgataag tgggaaggac tatattcaaa
     4681 ggtgaaagca ctcgttaagt atatgaaaaa ttctatagaa acttctctca attaggctaa
     4741 ttttattgca ataacaggtg cttacttttc tggagttctt tagcaaattt ttttattagc
     4801 tgaacttagt attagtggcc atactcctcc aatccaaagc tatttagaaa gattactata
     4861 tcctcaaaca ggcggtaacc ggcctcttca tcgggaatgc gcgcgacctt cagcatcgcc
     4921 ggcatgtccc cctggcggac gggaagtatc cagctcgagg tcgggccgcg ttgctggcgt
     4981 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5041 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5101 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5161 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5221 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5281 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5341 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5401 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     5461 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5521 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5581 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5641 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     5701 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     5761 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     5821 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     5881 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     5941 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6001 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     6061 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6121 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6181 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6241 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6301 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     6361 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     6421 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     6481 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     6541 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     6601 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     6661 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     6721 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     6781 gtatcacgag gccctttcgt cttcaagaat t
//
