LOCUS       sequence-405395-        1556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the Sma ES3 insert with a chloramphenicol
            resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo36
FEATURES             Location/Qualifiers
     source          1..1556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     389..408
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(475..577)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      636..1024
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      1323..1350
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(1371..1556,1..474))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGE"
ORIGIN
        1 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
       61 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
      121 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
      181 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
      241 tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
      301 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
      361 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
      421 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
      481 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
      541 attatggtga aagttggaac ctcttacgtg ccgatcagtc aattcgaggg ggatcaattc
      601 cgtattgatc cggccacgat gcgtccggcg tagaggatct gaagatcagc agttcaacct
      661 gttgatagta cgtactaagc tctcatgttt cacgtactaa gctctcatgt ttaacgtact
      721 aagctctcat gtttaacgaa ctaaaccctc atggctaacg tactaagctc tcatggctaa
      781 cgtactaagc tctcatgttt cacgtactaa gctctcatgt ttgaacaata aaattaatat
      841 aaatcagcaa cttaaatagc ctctaaggtt ttaagtttta taagaaaaaa aagaatatat
      901 aaggctttta aagcttttaa ggtttaacgg ttgtggacaa caagccaggg atgtaacgca
      961 ctgagaagcc cttagagcct ctcaaagcaa ttttgagtga cacaggaaca cttaacggct
     1021 gacatggggg tctcggagac ggtttagcct cgatcggctg ccagcccttg tcacgcctag
     1081 ccgggcaggg gatgcggctg aatgtcgggg ctatcgcttt ttcctgaaaa cacgcgtatc
     1141 gttcgaccca ctgtgtttgc tagggtcacc gtgaatcgtg gcctggtgca gttgatctca
     1201 cgatccgctc atcgatccgc tttaccaacg cctgcaactc agtctcggcc ccgatacccg
     1261 gtggctgcga tttttttcaa catgtgtttc aggagttagt aactattacg agacctcaca
     1321 ctggctcacc ttcgggtggg cctttctgcg tttataccta ggagtcaaaa ttactccccg
     1381 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctaccgac atggaagcca
     1441 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     1501 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtt
//
