LOCUS       sequence-405401-        2717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Receiver cloning vector for odd-1 level constructs. Contains lacZ 
            for blue/white screening..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pCA_o-1
FEATURES             Location/Qualifiers
     source          1..2717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    274..295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(310..315,316..333,334..340)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    348..364
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     353..375
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     372..388
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     372..388
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             384..707
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    401..457
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(458..475)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(458..474)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(467..489)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(683..702)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(965..1553)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1045..1064)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1641..2456)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1641..2456)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2364..2383
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
       61 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
      121 gagtggtgat tttgtgccga gctcattact cgcatccatt ctcaggctgt ctcgtctcgt
      181 ctccgagctc ttcaatgagg agtgagacca ttgacagcgc agctggcacg acaggtttcc
      241 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
      301 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
      361 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttgcat gcctgcaggt
      421 cgactctaga ggatccccgg gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc
      481 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
      541 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
      601 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
      661 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
      721 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
      781 acagacaagc tgtgacacta gtgagcacgg tctcacgcta gcatgaagag caccccagga
      841 tacatagatt accacaactc cgagcccttc cacccacaga atcaggggat aacgcaggaa
      901 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1381 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     1441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1621 tggtcatgca ttctaggtga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
     1681 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
     1741 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
     1801 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     1861 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc
     1921 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     1981 ccgttattca ttcgtgattg cgcctgagcg agtcgaaata cgcgatcgct gttaaaagga
     2041 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     2101 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc tgggatcgca
     2161 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     2221 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     2281 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcggtagatt
     2341 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     2401 atgttggaat ttaatcgcgg ccttgagcaa gacgtttccc gttgaatatg gctcataaca
     2461 gaacttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
     2521 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
     2581 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
     2641 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
     2701 ccgcgagatc atccgtg
//
