LOCUS       sequence-405420-        2680 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 54983 from 
            Phaeodactylum tricornutum with AB overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AB-NR
FEATURES             Location/Qualifiers
     source          1..2680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1330..1918)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1410..1429)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2009..2680,1..120))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc
       61 gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat
      121 gcgaaacgat cctcatcctg tctcttgatc agatattgat cccctgcgcc atcagatcct
      181 tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc
      241 cccagctggc aattccggtt cgcttgctaa gcttgcatgc ctgcaagtcg actctagagg
      301 agcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg
      361 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa
      421 catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg
      481 gctgcctgta tcgagtggtg attttgtgcc gagctcatta ctcgcatcca ttctcaggct
      541 gtctcgtctc gtctcaggtc tccggaggac agtcaccaac ggaatgcttt cgttaagagc
      601 aaggcgacaa cggttccttc gttgagatgg tggttccact gtgcaggtca cgtagtgaag
      661 tgatgttgcg gtagtctgca atcccgctag atacacgtca ttcgtgaaag gcctgttgtt
      721 ttcatgtccg ggtctgcacg ggcactagaa ggaaggaagc aaagacgaac ggaagtgact
      781 gtaaacgaga agtgcacgaa gccttttctt gtgacgtcac aacccgaata gccctacgtg
      841 gcgtgcgact ttccggcact ttggatatcg ttgccctata tgtattgggt gtatacatgt
      901 cgtattccaa ctacgacaga aaacagtcag ttacgggtat tcgaacttcc ggcacctgca
      961 gcgaggagtt ttgtgtccgt cgcacatcct ccgtgctttc ggcacctacg taggcggcaa
     1021 acttcctgcc ttccgccccc gccttgcggc attccggctc cgggccagaa ttgcccgggt
     1081 gttcacaatt ttgcctcctc acgaaaaaac gttctacttt gtattttggt cgggtttcgg
     1141 atccttccag caaccattct cattcaaagt caccacttgt gcgaacgtac tcgagaccac
     1201 caggatacat agattaccac aactccgagc ccttccaccc acagaatcag gggataacgc
     1261 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1321 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1381 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1441 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1501 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1561 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1621 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1681 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1741 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1801 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1861 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1921 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1981 gattttggtc atgcattcta ggtgattatt atttgccgac taccttggtg atctcgcctt
     2041 tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt
     2101 gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct
     2161 ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc
     2221 aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt
     2281 tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa
     2341 agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca
     2401 agatagccag atcaatgtcg atcgtggctg gctcgaagat accagcaaga atgtcattgc
     2461 gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt
     2521 cgtgcacaac aatggtgact tctacagcgc ggagaatctc actctctcca ggggaagccg
     2581 aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca
     2641 ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc
//
