LOCUS       sequence-405422-        3566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 49202 from 
            Phaeodactylum tricornutum with AB overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AB-P49
FEATURES             Location/Qualifiers
     source          1..3566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(2720..3308)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2800..2819)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3399..3566,1..624))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
       61 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt
      121 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
      181 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
      241 gtcgatcgtg gctggctcga agataccagc aagaatgtca ttgcgctgcc attctccaaa
      301 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
      361 gacttctaca gcgcggagaa tctcactctc tccaggggaa gccgaagttt ccaaaaggtc
      421 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
      481 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
      541 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
      601 ttcggcgatc accgcttccc tcatgcgaaa cgatcctcat cctgtctctt gatcagatat
      661 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
      721 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctaagcttgc
      781 atgcctgcaa gtcgactcta gaggagcggt gatcacaggc agcaacgctc tgtcatcgtt
      841 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
      901 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
      961 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctc
     1021 attactcgca tccattctca ggctgtctcg tctcgtctca ggtctccgga gtccaaacgg
     1081 tacgtcagat cccacgtcac taccgcaccc ggttattcca gtgcgtaagc tcgtagtgga
     1141 agaacaggct gcggtagtga cgtggaattt gacactttcc aatcttcaag acctctagcc
     1201 agcaaagact tcttgaatct gcaaggatat tctatttaga tataagtata tacttgacag
     1261 tgaggggggt cgtgaccgtc tctcgctggg atagagaaga tggcaaccat tgaattgaga
     1321 actcgaaact tgagcttggt tggatcttga tcattctgaa tttgcaggat aggttaacag
     1381 taaaagtcaa tcttgcgctc gccatctacc agaaagcacg tgggtgccca gatcctccca
     1441 acgagcgaac ggacggatgc tagtcggggg gatctctgtg ggccattcaa acgaaaggca
     1501 gatggcatcg ttccaagatc tattggtcat tgggcttctc atacagtcat tgacaccacg
     1561 attgaagaca aatttttgtt gacagtgaaa tcgttgcgga cgcgctccga ttcaaaagtt
     1621 tccctactcg gtcgcgtcaa ccgaccttcc cctcgtgagt ttacagcttg atacgcccta
     1681 tttggcttcc tatgtaaagt ctccagtgat catattaaag tcattggtca tgacactctc
     1741 cttgttcaaa atttggtacc atacaatgcg cggagcagtc agttctcatt tccagcgatc
     1801 gttaaaacaa ttagagaaat gccttacttc tctctttata tctgtattac tcagagtcag
     1861 tgagtagtac gaatttccaa cgtcctagct ccaatttatg gtttcaaaca atccgaattc
     1921 gttggtccac agtcaaggaa ttcgcaagaa tccctatttc actaggcgtg cactatgggg
     1981 tcgcttttga ggttctcatt tgcggcgatc gttaaaacga ttacagaaat gctacacttc
     2041 gctctttata tatattactc attgagcatc aggaatgtcc aatgtcgtaa ctccgattca
     2101 tggtttcaaa caatccgaat tcgttggtcc gaggaatccg caaaaagtaa ttgtagtcta
     2161 tgtacgaccg agtcgctggt gagggttttt tgtcgttgga ttgctcgagc gaattctccg
     2221 agattgtaca gaaagctcaa ctttcggaag gcatctatgt ggtccaacca atctattcaa
     2281 atatatcaat tgttaagagc agagttcgat cctcactatg tggaggggca tggtgagatt
     2341 acggagtgcc gagacataag agctccctgc aatcaagttg atggacatgc ttcgaaaatg
     2401 ccatgactga gaacgagaac gcccagatgc tccgcggcac gagaactcga tgaacacgaa
     2461 ccgaatccaa cgccaaaaac gcgttgtcca aaacattgca actctctctg ataaaaacca
     2521 aaacgccgca aattgcacca gcttttttgg ttgtcctacg cctctttccg caccacctac
     2581 tcgagaccac caggatacat agattaccac aactccgagc ccttccaccc acagaatcag
     2641 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2701 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2761 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2821 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2881 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2941 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3001 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3061 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3121 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3181 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3241 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3301 gatctcaaga agatcctttg atcttttcta cggggtctaa cgctcagtgg aacgaaaact
     3361 cacgttaagg gattttggtc atgcattcta ggtgattatt atttgccgac taccttggtg
     3421 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
     3481 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
     3541 ggcaggcgct ccattgccca gtcggc
//
