LOCUS       sequence-405423-        3063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 54499 from 
            Phaeodactylum tricornutum with AB overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AB-OEE3
FEATURES             Location/Qualifiers
     source          1..3063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             415..1206
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1297..1885
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1786..1805
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agaatggatg cgagtaatga gctcggcaca aaatcaccac tcgatacagg cagcccatca
       61 gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg
      121 atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga
      181 gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcac cgctcctcta
      241 gagtcgactt gcaggcatgc aagcttagca agcgaaccgg aattgccagc tggggcgccc
      301 tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc
      361 tgatggcgca ggggatcaat atctgatcaa gagacaggat gaggatcgtt tcgcatgagg
      421 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
      481 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
      541 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
      601 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagtgagatt
      661 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
      721 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc tggtatcttc
      781 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
      841 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
      901 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
      961 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     1021 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     1081 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     1141 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
     1201 aaataataat cacctagaat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1261 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1321 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1381 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1441 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1501 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1561 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1621 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1681 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1741 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1801 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1861 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     1921 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtgggtgg
     1981 aagggctcgg agttgtggta atctatgtat cctggtggtc tcgagtaggt tacagttgat
     2041 gtgcaatgga tccaaattga atgctgtgct gctcgtcgta tggctgctgc tggatgcaaa
     2101 ggatgctgtg ttgacgtcct atcttgctgg gtttcgtatc aattgcatgc gtatccgcaa
     2161 tgttcaacct caaggaattc gactatttca gtcccaatct caagccattc cctcggagcg
     2221 aagaccagac catttcttgt agtccgtcct tagacagtct ctaccaacgt ttttacggaa
     2281 tttggcacta gactatcatt ctctgaaatt ttcagcgaag tgtcgcgtct agactttacg
     2341 caaactgtaa acaattaaag atgcaagcag attcaacaaa tagcgacgga atgacgagaa
     2401 gagtcaagaa aatactagcc cattacaaag agtgtttgtg ttgaatgctc cgtaggatgg
     2461 ctaactgtaa ggtgtgattg gtatgagcgt cgcaacgcca cctcaacgtc atcatctttc
     2521 gtcggatttc cgtttatgga tactgctgcc cacagtcgtt ccaaaatttg tgatatacat
     2581 ggatattatt gataaatcat tctagatagc tcctcgtaaa agttactaat tgagtaggaa
     2641 tttttgttag tgtactctgg tttgtcttgt gtgaacggaa ctgaaaccaa tcttccggcg
     2701 cattcgcaga aatgcaacag atattggata gtagtgacac cgttgtcgga caaaaggcat
     2761 aaacatcgaa ctctctaagc ttcccgtcca atcgagggca ttttggatac gtacacggtt
     2821 tgcccggcaa tcatacccat acagctgatt ccaatataag ttgtaaacgt gaacaaaaaa
     2881 aagaacatga tacctgcaag aatattgggc gtcatctgca catagtaaac tccggagaga
     2941 tcctggttgt tgttgccgtc agctgcctga tcgagacgtc gggcgttgga tgccaacata
     3001 ctgcttccag ctttctgcat ggcctgcata ctcctgagac ctgagacgag acgagacagc
     3061 ctg
//
