LOCUS       sequence-405428-        3459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 51305 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-BCA
FEATURES             Location/Qualifiers
     source          1..3459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1833..2421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1913..1932)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2512..3303)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 atgcctgcaa gtcgactcta gaggagcggt gatcacaggc agcaacgctc tgtcatcgtt
       61 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
      121 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
      181 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctc
      241 attactcgca tccattctca ggctgtctcg tctcgtctca ggtctcagga gtgtgtacga
      301 tccagagcat ccactactcc catcttgata acattctcac cagcagaaat cttgcgttcc
      361 aattccatca aggtaactgc tgctgatcga caaggtatag caactacctt tcctaaaatg
      421 tcaacaccgg gagcagctga tagaatcgat ggtgcacgaa taaatactcc aggaaaagaa
      481 ccactctgct ccatgccggg tggtgaaggt gtttccattt caaaactcga cacctgagaa
      541 ccaaagtaat ttcggcacac tgtgatatct ataccaccaa tgagtgcctg gccgtcttca
      601 atcaccgcgg aggttcccac gcacttgtca gcaagtaaaa tcatcccagc acaagtcccc
      661 cacgttggtt tagatcgtga gaattcccgc aaagcttccc acatggtttt tccggacgaa
      721 tctttcccag atgaggttcc gatcaatccc atggcggtgg attctccacc tggcaatacg
      781 atgccatcga ggccttccaa attcatggga gtccgtacct acagaagaaa acaagataaa
      841 cataaaataa gatcgattcg tagccagagg caaatgatcg atcaacattg gtgcaactca
      901 atgtcgcgtc tcgtacctgc actgttcggc aaccaaccgc ttccaagcat ttttgatgat
      961 cttcaaacgc gccttgaaca gcaagaaccc caatgagagg ctctgccatt ttagccacgg
     1021 atctttgata gcctctgaac cgcttcgtga gggatgttga caaaaatacc cttacctgaa
     1081 cctcccgctt cttatgatgg caattgccaa acttcctcgg tcggtttcgg aatgccgact
     1141 cgtaggtacc gcatagtcgt gcaaaagttt cgtctcactc gtgagacatc gacaggcgag
     1201 atgtctccgg ccccacccta ctagtatcaa tgatcagaat cgatcaagcg ctgcctgacg
     1261 cagaacgaca tctgacttcg atcttgacca aagacactga agatttaaca attatgcgtc
     1321 atcgatgagt cacattatac acttcaagat ttcagcttcc ttttctggaa gacgtcagca
     1381 gagtgaattc gaagacaagt agaagacttc gacttaccaa tgacttctat atcgctttca
     1441 tatctataaa ttgtaataca gagtggacga tgataaatga acttccttta acggttttaa
     1501 aaaacgtatt taagggatat tatcatcaag atacccggaa aggtgacgtt tttcctcgaa
     1561 tacgtcacga tgccgtaggg agtgacgcac gagcttcatc atcgatcttg acggcaaaat
     1621 acattctcac agctcaccga ccatcttgaa actgtttcat cacaattcct agcagaaaat
     1681 catcgaaaca aatgcgagac caccaggata catagattac cacaactccg agcccttcca
     1741 cccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1801 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1861 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1921 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1981 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2041 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2101 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2161 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2221 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     2281 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2341 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2401 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2461 tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtgatt attatttgcc
     2521 gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg
     2581 cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg
     2641 ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat
     2701 tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc
     2761 gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag
     2821 atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct
     2881 atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa
     2941 gataccagca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg
     3001 ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat
     3061 ctcactctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt
     3121 tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag
     3181 gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg
     3241 ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct
     3301 catgcgaaac gatcctcatc ctgtctcttg atcagatatt gatcccctgc gccatcagat
     3361 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     3421 cgccccagct ggcaattccg gttcgcttgc taagcttgc
//
