LOCUS       sequence-405429-        2588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 47740 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-47740
FEATURES             Location/Qualifiers
     source          1..2588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             880..1671
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1762..2350
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2251..2270
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctttactga cgaacatggt caatggagag ggagtgaggc gtcgtcggtt gcgggctgcc
       61 gtatcgactc ttccggatgc gtatacggtc cggtgtacgt agatatgaac gtaccaagga
      121 aggtccgtca atgaactgtc ggtacgcctt cctgagcacc caaataccct aagttttcta
      181 ccaatgatag ggttcaagga ctaacgtaac gagtcggtcg acaattcttt cccagaaatt
      241 cttctcgcag taggaataca atgtcactaa ctagtagagt atatttactg ttgactgcaa
      301 acagagacga gcacgtacta gcacaaaatc aatcctggag aactctctaa ctccaccgaa
      361 agaagtatcc cttgtgaatt tccgttgctt caattcgatc gagcgcgtcg ttttgttgga
      421 tgaagctggg ccctcctgag acctgagacg agacgagaca gcctgagaat ggatgcgagt
      481 aatgagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
      541 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
      601 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
      661 tgtaacgatg acagagcgtt gctgcctgtg atcaccgctc ctctagagtc gacttgcagg
      721 catgcaagct tagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg
      781 aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga
      841 tcaatatctg atcaagagac aggatgagga tcgtttcgca tgagggaagc ggtgatcgcc
      901 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
      961 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
     1021 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     1081 aacgaccttt tggaaacttc ggcttcccct ggagagagtg agattctccg cgctgtagaa
     1141 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     1201 caatttggag aatggcagcg caatgacatt cttgctggta tcttcgagcc agccacgatc
     1261 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     1321 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     1381 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     1441 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     1501 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     1561 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     1621 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata ataatcacct
     1681 agaatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1741 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1801 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1861 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1921 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1981 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2041 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2101 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2161 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2221 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2281 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2341 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2401 tgctcacatg ttctttcctg cgttatcccc tgattctgtg ggtggaaggg ctcggagttg
     2461 tggtaatcta tgtatcctgg tggtctcgca tttatagtcg agagtgagaa tagaaagagg
     2521 ctggtgaccc tgagcgctag tacaaacgac taaattggag attcgatgca gaactaacag
     2581 taagccag
//
