LOCUS       sequence-405430-        3114 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 41365 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-GEF
FEATURES             Location/Qualifiers
     source          1..3114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1772..2360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1852..1871)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2451..3114,1..128))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
       61 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
      121 tccctcatgc gaaacgatcc tcatcctgtc tcttgatcag atattgatcc cctgcgccat
      181 cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca
      241 gagggcgccc cagctggcaa ttccggttcg cttgctaagc ttgcatgcct gcaagtcgac
      301 tctagaggag cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
      361 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
      421 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
      481 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctcattact cgcatccatt
      541 ctcaggctgt ctcgtctcgt ctcaggtctc aggagtcgtg tgaccagcag cacttcaacc
      601 gctgtcagtg cgaactacag caaagcccct gtggaccatg ttgagactca gtattgcgag
      661 gatcgctgca atgaccaatt catgtcactc ttgctcgtac cagatcaata cttcagcacg
      721 gtaccgtctt gaagaaattc ttttccaggt agcgctcaag cagctagagt atttcaagtc
      781 taagctcttg atgtattcga gaagcaagct ccgtgtttcc ctgtattgct gagctgccat
      841 tattcgcagc taaagttgcg ctgagtctgg tcgactagcc ctacactact cttaagaagc
      901 tggacatgta taaacagaga caaaccattg cattagtaga tgcgcttctt tgctatttct
      961 aaaacaatca attgggaaca tagaggatat gaaacgagca aaatgtgacg agagcatact
     1021 tcaccggcgc cttgacgctc gcaaactcgg gtaccgaaca aggcagattc tgaagccagc
     1081 cccccactgg taggcgagat gctgtcaatt ttgttatggt gggaatggaa aagtgtacca
     1141 tggaaggaac gatgctgggt atcctgagga atagagcgaa ctaacagtaa gtgtaagcat
     1201 ttgttttcaa tcatatctcc ctttacagtc agcctttttt attcgatttt tcaaggatta
     1261 ttttgaatta gggttgaaag tcaaattgtt cccgagcaat gttgtgatca taccggtagt
     1321 ttccttcacc gtcagtctct cgctgttctg ttctcatgtt tcgaaggtta gtgtggcacc
     1381 aaccgcaact gacatcatca aaaacgagaa aggccattga cacgtactat gatagaaatt
     1441 ctactgcaac caatttccct aggataaaat gtgcgcagtc gatagacgga cgcattctac
     1501 cttttcgcac gactgatttt gagcagatca tcaatgaacg aaacatctac cacacattcc
     1561 ggatttctcc actttattgt taggtatctc tattttctcc ggaatttccc gtaccagcaa
     1621 gttggagaca atgcgagacc accaggatac atagattacc acaactccga gcccttccac
     1681 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1741 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1801 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1861 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1921 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1981 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2041 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2101 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2161 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2221 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2281 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2341 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2401 ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtgatta ttatttgccg
     2461 actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc
     2521 gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc
     2581 tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt
     2641 ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg
     2701 ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga
     2761 tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta
     2821 tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag
     2881 ataccagcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga
     2941 taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc
     3001 tcactctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt
     3061 gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggc
//
