LOCUS       sequence-405433-        2654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing the promoter from gene 54730 from 
            Phaeodactylum tricornutum with AC overhangs used to build a level 1 
            construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_AC-54730
FEATURES             Location/Qualifiers
     source          1..2654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      771..1359
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1260..1279
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2543..2654,1..680)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
       61 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
      121 gagagagtga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
      181 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
      241 ttgctggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
      301 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
      361 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
      421 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
      481 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
      541 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
      601 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
      661 ccaaggtagt cggcaaataa taatcaccta gaatgcatga ccaaaatccc ttaacgtgag
      721 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      781 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      841 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      901 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      961 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1021 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1081 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     1141 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1201 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1261 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1321 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     1381 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     1441 gattctgtgg gtggaagggc tcggagttgt ggtaatctat gtatcctggt ggtctcgcat
     1501 tagcttgttt gtcggtacgt gccagagtgg aggcccgtcg tatacactat tctagatgaa
     1561 agccagaacg aacgaacgaa cgcgttagac gatgtatccg ctatccgatc cgaaaaagac
     1621 gagagagaag gacctgcgtt ggtcctaaca ctcttttttc tagtcgccac gacaatgtac
     1681 gaatcaattg ttctgttccc aaaagcaagg acaaaccgag caactaccta tctagctaga
     1741 tttgggttcg tgccgtctcc ttcgcctaac gtcctcgcag tcaggccgtt tgcattctcg
     1801 cacgcgttga agcgcgttgt gtgtgagaga taattgtgtc gatttgtcgc gcattagccg
     1861 agagagggta gggctcataa actgacgacg tacaggcttt tactggcctc aaagtcaaag
     1921 tcacctgtaa atgacatcat tatcgtggat gacggtcgtt gttgaacctt acatgaccgg
     1981 atagagcagg aaaccgggtc tttccaacgc ctgcgccagg gctcgattct tcattttgta
     2041 atcaagcgat ctaaccgcgt aggttgtctg acgttagaca tgattccttc tccgactcct
     2101 gagacctgag acgagacgag acagcctgag aatggatgcg agtaatgagc tcggcacaaa
     2161 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
     2221 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc
     2281 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc
     2341 gttgctgcct gtgatcaccg ctcctctaga gtcgacttgc aggcatgcaa gcttagcaag
     2401 cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa
     2461 ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaatat ctgatcaaga
     2521 gacaggatga ggatcgtttc gcatgaggga agcggtgatc gccgaagtat cgactcaact
     2581 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     2641 gtacggctcc gcag
//
