LOCUS       sequence-405439-        4386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF545 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF545
FEATURES             Location/Qualifiers
     source          1..4386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1697..1796)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1813..1832)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1814..1832)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1837..1853)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1837..1853)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2284..3075
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3168..3756
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3657..3676
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3910..3927
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4086..4113)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4205..4291)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4340..4362
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4354..4371
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4355..4371
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggcccttcg atcagtgatg
      121 ttcagtgatg tatccataga cttctctcca gaagagtggg aatacctgga cttggaacaa
      181 aaggacttgt acagagatgt catgttggag aactacagca acttggtctc actgggatgc
      241 ttcatttcta aaccagatgt gatttcctca ttggagcaag gaaaagagcc ttggaaagtt
      301 gtgaggaaag gaagaagaca atatccagat ttggagacca agtatgagac caagaagtta
      361 tctttagaaa atgacattta tgaaataaat ttatcccagt ggaagataat ggaaagaatt
      421 gaaaaccatg gccttaaggg tctcatttta aaaaatgatt gggaatccac aggaaaaatt
      481 gaaggacagg agagacctca agaaggatac ttcagtagtg tgaaaatgcc atctgaaaag
      541 gtgtcctctt accagaaacg cacatctgtt actccacatc agagacttca ttttgttgat
      601 aaaccctatg aatgtaagga atgtgggaag gcgttcagag tgcgccaaca gcttactttt
      661 catcacagaa ttcatactgg tgaaaaaccg tatgaatgta aggaatgtgg gatggccttc
      721 agacagactg cacaccttac tcgacatcag agacttcatt ctggtgaaaa actctatgaa
      781 tgtaaggaat gtggggaagc tttcatatgt ggtgcagatc ttagagtaca tcagaaaatg
      841 catattggtg agaagcccta tgaatgtaaa gaatgtggga aggcttttag ggtacgagga
      901 caacttactc tgcatcagag gattcatact ggtgagaaac cctatgtgtg taaagagtgt
      961 ggaaaagcct ttagacagta cgcacacctg actcggcatc agaagcttaa tagtgctgac
     1021 aggctctatg aatgcaaaga atgtgggaag gcctttttgt gtggctctgg tcttagagta
     1081 catcacaaac ttcatactgg tgagaaaccc tatgaatgta aggaatgcgg gaaggccttt
     1141 agagtgcgac aacaactaac actccatcag agaattcata ctggtgagaa accctatgaa
     1201 tgtaaggaat gtggaaagac ctttagccgt ggctatcatc ttattctcca tcacagaatt
     1261 catactggtg aaaaacctta cgaatgtaag gaatgctgga aagcctttag tcgctactca
     1321 caacttattt cacatcagag tattcatatt ggtgttaagc cctatgactg taaggaatgc
     1381 gggaaggcct tcagactact ttcacaactc acacagcatc agagtattca tattggtgag
     1441 aaaccttata aatgtaagga atgtggcaag gcctttagat tgcgccaaaa acttactcta
     1501 catcagagca ttcatactgg cgaaaaaccc tttgagtgta aggaatgtag gaaggccttt
     1561 agacttaatt catcccttat tcaacatctg agaattcatt ctggtgagaa accctatgaa
     1621 tgtaaggaat gtaagaaggc ctttaggcaa cattcacacc ttactcatca tctgaaaatt
     1681 cataatgtaa aaatctaccc aactttcttg tacaaagttg gcattataag aaagcattgc
     1741 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     1801 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     1861 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc
     1921 ctcctctaga ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg
     1981 tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt
     2041 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     2101 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
     2161 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     2221 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     2281 attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     2341 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     2401 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     2461 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     2521 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     2581 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     2641 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     2701 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     2761 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     2821 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     2881 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     2941 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     3001 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
     3061 gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg
     3121 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3181 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3241 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3301 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3361 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3421 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3481 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3541 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3601 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3661 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3721 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3781 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3841 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3901 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3961 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     4021 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     4081 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     4141 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     4201 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     4261 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     4321 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     4381 gggccc
//
