LOCUS       sequence-405440-        4863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF770 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF770
FEATURES             Location/Qualifiers
     source          1..4863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2174..2273)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2290..2309)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2291..2309)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2314..2330)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2314..2330)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2761..3552
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3645..4233
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4134..4153
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4387..4404
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4563..4590)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4682..4768)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4817..4839
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4831..4848
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4832..4848
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgatggctga aaacaattta
      121 aaaatgctaa agattcaaca gtgtgtggta gccaacaaac tacctagaaa caggccatat
      181 gtttgcaata tttgttttaa gcactttgaa acaccatcaa aattagctag gcactatctc
      241 attcatactg gtcaaaagcc atttgaatgt gatgtgtgtc ataaaacctt tagacaacta
      301 gttcatctgg agaggcatca actaactcat agtctgcctt ttaaatgtag tatttgtcag
      361 cgtcacttta aaaatctgaa gacatttgtg aagcaccaac aacttcacaa tgaaacctat
      421 cagaataatg ttaaacaggt cagaagattg ctggaggcca agcaagaaaa gtcaatgtat
      481 ggagtgtata atacttttac cacagaggaa agatgggcat tacacccgtg ctctaagtct
      541 gatcccatgt atagcatgaa aagaagaaag aatattcatg catgtacaat ctgtggcaag
      601 atgtttccat cacagtcaaa acttgatagg catgtactta ttcatactgg tcagaggcct
      661 tttaaatgtg tcttgtgtac taaatctttt cgacagtcaa ctcacttaaa aatccaccaa
      721 cttacacatt cagaagaaag accttttcaa tgttgttttt gtcaaaaagg atttaagatt
      781 caaagcaaac ttctgaagca taaacaaatc catactagga ataaggcttt tcgggctctt
      841 ttattaaaga agaggcgtac agaatctcgc cccctgccta ataagttaaa tgcaaatcag
      901 ggtggttttg aaaatggtga gattggtgaa tctgaggaga ataatccact tgatgtccac
      961 tcaatttata ttgtcccttt tcaatgtcca aagtgtgaaa agtgttttga atcagagcag
     1021 attctcaatg aacacagctg ttttgctgct agaagtggca aaattccaag caggttcaaa
     1081 agaagctaca actataaaac cattgttaaa aaaatcttgg ccaagcttaa gcgtgctagg
     1141 agtaaaaaat tagataactt tcaatctgag aaaaaagttt ttaaaaagag tttcttgaga
     1201 aattgtgatc ttatttctgg tgagcagagc tctgaacaaa cccagagaac atttgtgggt
     1261 tctcttggca aacatggaac atataaaaca attggcaata gaaagaagaa aacattgact
     1321 ttgccatttt cttggcaaaa tatgggaaaa aatttgaaag gcatccttac gacagaaaac
     1381 atattaagca ttgataattc agtgaataag aaagacttgt caatctgtgg ttcatcaggt
     1441 gaggaattct ttaataactg tgaggtactt cagtgtggtt tttcagttcc aagggaaaac
     1501 atacgtacta gacataagat atgtccttgt gacaaatgtg agaaggtatt tccttctata
     1561 tccaaactaa aaagacacta tttaattcat actggacaga ggccctttgg ctgtaatatt
     1621 tgtgggaaat cttttagaca gtcagctcac ttaaaaagac atgaacagac tcataatgaa
     1681 aagagtcctt atgcatctct ttgccaagta gaatttggaa acttcaacaa tctttctaat
     1741 cattcaggta ataatgttaa ctataatgct tcccaacaat gtcaggctcc tggtgttcaa
     1801 aaatacgagg tctcagagtc agatcaaatg tcaggagtta aggcagagtc acaggatttt
     1861 attcctggta gcaccgggca accctgtctt cctaatgtac ttttggaatc agagcaaagc
     1921 aatccttttt gcagttattc agagcatcag gagaaaaatg atgtcttcct gtaccgatgc
     1981 agtgtttgtg ctaaaagttt ccgatctcca tctaaactgg aaagacacta cctaattcat
     2041 gcagggcaga aaccatttga atgctcagtt tgtggcaaaa cattcagaca ggctcctcac
     2101 tggaagagac atcagcttac tcactttaaa gaacgaccac aagggaaagt ggttgcctta
     2161 gattcggtta tgtacccaac tttcttgtac aaagttggca ttataagaaa gcattgctta
     2221 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     2281 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     2341 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc
     2401 ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga
     2461 cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt
     2521 aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     2581 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
     2641 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     2701 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt
     2761 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     2821 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     2881 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     2941 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     3001 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     3061 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     3121 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     3181 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     3241 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     3301 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     3361 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     3421 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     3481 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
     3541 gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg
     3601 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3661 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3721 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3781 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3841 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3901 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3961 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4021 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4081 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4141 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4201 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4261 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4321 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4381 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     4441 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     4501 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     4561 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     4621 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     4681 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     4741 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     4801 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     4861 ccc
//
