LOCUS       sequence-405441-        4911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF132 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF132
FEATURES             Location/Qualifiers
     source          1..4911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2222..2321)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2338..2357)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2339..2357)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2362..2378)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2362..2378)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2809..3600
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3693..4281
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4182..4201
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4435..4452
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4611..4638)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4730..4816)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4865..4887
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4879..4896
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4880..4896
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca ccatggccct gcccagccca
      121 caggttctaa tggggttgcc agcgttgctg atgggcccgg cgcagcatac ttcttggccc
      181 tgtggctcag ctgtccccac attgaagagc atggtaacct ttgaagatgt ggctgtatac
      241 ttctcccaag aggagtggga gctccttgat gcagcccaga ggcacctgta ccacagtgtg
      301 atgctggaaa accttgagct tgtgacctca cttggttctt ggcatggagt agagggtgag
      361 ggggcccatc ccaagcagaa tgtttctgta gaagtgttac aggtcaggat ccctaatgca
      421 gatccttcca ccaagaaagc taactcctgt gacatgtgtg ggccattctt gaaagacatt
      481 ttgcacctgg ctgagcatca gggaacacag tctgaggaga aaccctacac atgtggagca
      541 tgtgggagag acttttggtt gaatgcaaac cttcaccagc accagaagga gcacagtgga
      601 gggaagccct ttagatggta caaggacagg gacgcactta tgaagagctc taaagtccac
      661 ctgtcagaga accccttcac ttgcagggaa ggtgggaagg tcatcctggg cagctgtgac
      721 ctcctccagc ttcaagctgt tgacagtggg cagaagccat attccaatct tgggcagctt
      781 ccagaagtct gtaccacaca gaaactcttc gagtgcagca actgtggaaa agccttcctg
      841 aagagctcca ctctccccaa ccatctgaga actcactctg aagagatacc atttacatgc
      901 ccaacaggtg gaaatttctt agaggagaaa tcaatccttg gtaataaaaa atttcacact
      961 ggggaaatac cccatgtgtg taaggagtgt gggaaggcct ttagtcactc atctaagctg
     1021 aggaagcacc agaaatttca cactgaagta aaatattatg agtgcattgc atgtgggaaa
     1081 accttcaacc acaaactcac atttgttcat catcagagaa ttcactcagg agaaagacct
     1141 tatgagtgtg atgaatgtgg gaaagccttc agtaacagat cacacctcat tcggcatgag
     1201 aaagttcaca ctggagaaag gccttttgag tgcctgaaat gtggaagagc cttcagccaa
     1261 agctccaatt tccttcggca tcagaaagtt cacacacagg taagacctta tgagtgcagt
     1321 caatgtggta aatccttcag ccgaagctct gctctcattc agcactggag agttcacact
     1381 ggagaaagac cgtatgaatg cagtgaatgt ggaagagctt ttaacaataa ctccaacctt
     1441 gctcagcacc agaaagttca caccggagaa cggccttttg agtgcagtga atgtggaaga
     1501 gacttcagcc aaagctccca tctccttcga catcagaaag ttcacactgg agaacggcct
     1561 tttgaatgct gtgattgtgg taaagccttc agtaatagct ccaccctcat ccagcaccag
     1621 aaagtacata ctgggcaaag gccttatgag tgcagcgaat gtaggaaatc cttcagccgc
     1681 agctccagcc tgattcagca ctggagaatt cacactggag aaaagcctta cgagtgtagt
     1741 gagtgtggga aagcctttgc tcacagctcc actctcattg aacactggag agttcacaca
     1801 aaagaaaggc cttatgagtg caatgaatgt gggaaattct ttagccaaaa ctccattctc
     1861 attaagcatc agaaagttca tactggagaa aagccttata aatgcagtga atgtgggaaa
     1921 ttctttagcc gaaaatccag ccttatttgt cactggagag ttcacactgg agaaaggcct
     1981 tacgaatgca gtgaatgtgg gagagccttt agcagtaact cccacctggt tcgtcatcag
     2041 agagttcaca cacaagaaag gccctatgag tgcatccagt gtggaaaagc ctttagtgaa
     2101 agatctacac ttgttcggca ccagaaagtt cacaccagag aaaggactta tgagtgtagc
     2161 cagtgtggga aactcttcag ccatctttgt aaccttgcac agcataaaaa gattcatacc
     2221 ttgccaactt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     2281 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     2341 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     2401 aatctctgat gttacattgc acaagataaa aatatatcat catgcctcct ctagaccagc
     2461 caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg
     2521 aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
     2581 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     2641 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
     2701 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     2761 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg
     2821 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     2881 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     2941 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     3001 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     3061 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     3121 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     3181 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     3241 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     3301 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     3361 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     3421 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     3481 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     3541 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
     3601 ccctcgagcc acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     3661 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3721 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3781 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3841 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3901 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3961 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4021 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4081 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4141 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4201 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4261 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4321 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4381 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4441 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     4501 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     4561 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     4621 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     4681 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     4741 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     4801 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     4861 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc c
//
