LOCUS       sequence-405442-        4491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC27A1_STOP
FEATURES             Location/Qualifiers
     source          1..4491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2048..2147)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2164..2183)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2165..2183)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2188..2204)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2188..2204)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2306..2325)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2317..3126
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2384..2403)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3273..3861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3762..3781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4015..4032
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4191..4218)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4310..4396)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4445..4467
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4459..4476
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4460..4476
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgag ggcacctgga
      121 gcaggagcag catccgtggt gtctctggcc ctgctgtggc tgctgggact gccatggacc
      181 tggtccgccg cagccgccct gggcgtgtat gtgggatctg gaggatggag gttcctgagg
      241 atcgtgtgca agacagcccg gagagatctg tttggcctga gcgtgctgat cagggtgcgc
      301 ctggagctga ggcgccacca gagggccggc cacaccatcc ccagaatctt ccaggcagtg
      361 gtgcagaggc agcctgagag actggccctg gtggatgcag gaaccggaga gtgttggaca
      421 tttgcccagc tggacgccta ctccaacgcc gtggccaatc tgttccggca gctgggattt
      481 gcaccaggcg acgtggtggc catcttcctg gagggcagac ccgagtttgt gggactgtgg
      541 ctgggactgg caaaggcagg aatggaggcc gccctgctga acgtgaatct gcggagagag
      601 cctctggcat tctgcctggg cacctccgga gcaaaggccc tgatctttgg aggagagatg
      661 gtggcagcag tggcagaggt gagcggacac ctgggcaagt ccctgatcaa gttctgttct
      721 ggcgacctgg gacctgaggg aatcctgcca gacacccacc tgctggatcc tctgctgaag
      781 gaggcaagca cagcaccact ggcacagatc ccttccaagg gaatggacga taggctgttc
      841 tacatctata ccagcggaac cacaggactg ccaaaggcag ccatcgtggt gcactcccgg
      901 tactatagaa tggccgcctt tggccaccac gcatatagga tgcaggcagc agacgtgctg
      961 tacgattgcc tgcccctgta tcactctgcc ggcaacatca tcggcgtggg ccagtgtctg
     1021 atctacggcc tgaccgtggt gctgaggaag aagttcagcg cctcccgctt ttgggacgat
     1081 tgcatcaagt acaattgtac agtggtgcag tatatcggcg agatctgccg gtacctgctg
     1141 aagcagccag tgagagaggc agagaggcgc caccgggtga gactggcagt gggaaacgga
     1201 ctgaggccag caatctggga ggagttcacc gagaggtttg gcgtgcgcca gatcggcgag
     1261 ttctatggcg ccacagagtg caactgtagc atcgccaata tggatggcaa ggtcggctcc
     1321 tgtggcttta actctaggat cctgccccac gtgtacccta tccgcctggt gaaagtgaat
     1381 gaggatacca tggagctgct gagagacgcc cagggcctgt gcatcccatg tcaggcagga
     1441 gagccaggac tgctggtggg acagatcaat cagcaggacc ccctgcggag attcgatggc
     1501 tacgtgagcg agagcgccac atccaagaag atcgcccaca gcgtgttttc caagggcgat
     1561 tctgcctacc tgagcggcga cgtgctggtc atggatgagc tgggctacat gtatttcagg
     1621 gaccgctctg gcgatacctt tcggtggaga ggcgagaacg tgtctaccac agaggtggag
     1681 ggcgtgctga gcaggctgct gggacagaca gacgtggccg tgtacggcgt ggcagtgcct
     1741 ggagtggagg gcaaggcagg catggccgcc gtggccgacc ctcacagcct gctggatcca
     1801 aatgccatct atcaggagct gcagaaggtg ctggcaccat acgcaaggcc catcttcctg
     1861 agactgctgc cacaggtgga caccacaggc accttcaaga tccagaagac aaggctgcag
     1921 agggagggat ttgacccaag gcagacctct gatcgcctgt tctttctgga cctgaagcag
     1981 ggccactatc tgcctctgaa cgaggccgtg tacacacgca tctgcagcgg cgcctttgcc
     2041 ctgtaagacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     2101 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     2161 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     2221 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     2281 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     2341 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     2401 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     2461 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     2521 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     2581 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     2641 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     2701 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     2761 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     2821 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     2881 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     2941 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     3001 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     3061 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     3121 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     3181 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     3241 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3301 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3361 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3421 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3481 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3541 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3601 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3661 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3721 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3781 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3841 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3901 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3961 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4021 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     4081 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     4141 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     4201 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     4261 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     4321 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     4381 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     4441 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc c
//
