LOCUS       sequence-405450-       10832 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac-based transposon vector plasmid which encodes the 
            expression units of liver-enriched transcription factor genes 
            (hHNF1-beta-hHNF4-alpha-hHNF6) under control of the TRE/PCMVmin 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10832)
  AUTHORS   Kitano H, Kawabe Y, Kamihira M
  TITLE     HepG2-Based Designer Cells with Heat-Inducible Enhanced Liver 
            Functions.
  JOURNAL   Cells. 2022 Apr 1;11(7):1194. doi: 10.3390/cells11071194.
  PUBMED    35406758
REFERENCE   2  (bases 1 to 10832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2022-04-1"; volume: "11(7):1194. doi"; pages: " 
            10.3390/cells11071194"
COMMENT     Sequence Label: 
            PB513B-1/TRE-hHNF1-beta-hHNF4-alpha-hHNF6-EF1-alpha-Bla
FEATURES             Location/Qualifiers
     source          1..10832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1368..1386
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1404..1422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1439..1457
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1475..1493
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1511..1529
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1546..1564
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1582..1600
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        1612..1650
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1647..1671
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1689..1698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3375..3431
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4869..4922
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5292..5321
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             5295..5324
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     promoter        6359..6570
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6583..6851
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     primer_bind     complement(7334..7354)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    7402..7523
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7439..7458)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7493..7512
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8286..8320
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8811..8827)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8811..8827)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8824..8846)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8835..8851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8859..8865,8866..8883,8884..8889))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8904..8925
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9042..9059)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9213..9801)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9293..9312)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9972..10763,10764..10832))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10595..10614
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gttttactcc ctatcagtga
     1381 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc
     1441 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
     1501 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata
     1561 gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt
     1621 gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaat
     1681 ctagagccgc caccatggtt tccaagctga ccagcctgca gcaagagctg ctgtctgctc
     1741 tgctgtctag cggcgtgacc aaagaggtgc tggttcaggc cctggaagaa ctgctgccct
     1801 ctcctaactt cggcgtgaag ctggaaaccc tgcctctgtc tcctggatct ggcgccgagc
     1861 ctgataccaa gcctgtgttt cacaccctga ccaacggcca cgccaagggt agactgagcg
     1921 gagatgaggg atctgaggac ggcgacgact acgacacacc tcctatcctg aaagagctgc
     1981 aggccctgaa cacagaggaa gccgctgaac agagggccga agtggacaga atgctgagcg
     2041 aggacccttg gagggccgcc aagatgatca agggctacat gcagcagcac aacatccctc
     2101 agcgcgaggt ggtggatgtg accggcctga atcagagcca cctgtctcag cacctgaaca
     2161 agggcacccc tatgaagacc cagaagcggg ctgccctgta cacttggtac gtgcggaagc
     2221 agagagagat cctgcggcag ttcaaccaga ccgtgcagag cagcggcaac atgaccgata
     2281 agagcagcca ggaccagctg ctgtttctgt tccccgagtt cagccagcag tctcacggac
     2341 ctggccagtc tgatgatgcc tgtagcgagc ccaccaacaa gaagatgcgg cggaaccggt
     2401 ttaagtgggg acctgcctct cagcagatcc tgtaccaggc ctacgaccgg cagaagaacc
     2461 ccagcaaaga agaaagagag gccctggtcg aggaatgcaa cagagccgag tgtctgcaga
     2521 gaggggtgtc cccatctaaa gcccacggcc tgggaagcaa cctggttact gaagtgcggg
     2581 tgtacaattg gttcgccaac cggcggaaag aagaggcctt cagacagaaa ctggccatgg
     2641 acgcctacag cagcaatcag acccacagcc tgaatcctct gctgagccac ggcagccctc
     2701 accaccaacc ttcaagcagc cctcctaaca agctgagcgg cgtgcggtat tctcagcagg
     2761 gcaacaacga gatcaccagc agctccacca tcagccacca cggcaatagc gccatggtca
     2821 caagccagag cgtgctccag caagtgtctc cagcttctct ggaccccggc cacaatctgc
     2881 ttagccccga cggaaagatg atctccgtgt ctggcggagg cctgcctcct gtgtctaccc
     2941 tgacaaacat ccatagcctg agccaccaca atcctcagca gtcccagaac ctgatcatga
     3001 cccctctgtc cggcgtgatg gctatcgccc agtctctgaa tacctctcag gcccagtccg
     3061 tgcctgtgat caattctgtg gccggatctc tggccgctct gcagcctgtt cagttttccc
     3121 agcagctgca cagccctcat cagcagcctc tgatgcagca gagccctggc tctcatatgg
     3181 cccagcagcc ttttatggcc gccgtgacac agctgcagaa cagccatatg tacgcccaca
     3241 agcaagagcc tccacagtac agccacacca gcagattccc cagcgctatg gtggtcaccg
     3301 acaccagctc tatcagcaca ctgaccaaca tgagcagcag caagcagtgc ccactgcagg
     3361 cttggggatc tggcgccacc aacttcagcc tgctgaaaca ggctggcgac gtggaagaga
     3421 accccggacc tatgagactg agcaagaccc tggtggacat ggacatggcc gattactctg
     3481 ccgctctgga ccctgcctac accacactgg aatttgagaa cgtgcaggtc ctgaccatgg
     3541 gcaacgacac atctccaagc gagggcacca acctgaacgc ccctaatagc ctgggagtgt
     3601 ctgccctgtg tgccatctgt ggcgatagag ccacaggcaa gcactacggc gccagctctt
     3661 gtgatggctg caagggcttc tttcggcgga gcgtgcggaa gaaccacatg tacagctgcc
     3721 ggttcagccg gcagtgcgtg gtggacaagg ataagcggaa ccagtgccgg tactgcagac
     3781 tgaagaagtg cttcagagcc ggcatgaaga aagaggccgt ccagaacgag cgggacagaa
     3841 tcagcaccag aagaagcagc tacgaggaca gcagcctgcc tagcatcaat gccctgctgc
     3901 aggccgaagt gctgagcaga cagatcacaa gccctgtgtc cggcatcaac ggcgacatca
     3961 gagccaagaa gatcgccagc attgccgacg tgtgcgagag catgaaggaa cagctgctgg
     4021 tgctggtgga atgggccaag tacatccccg ccttttgcga gctgcccctg gatgatcaag
     4081 tggccctgct gagagcacac gccggcgaac atttgctgct gggagccacc aagcggagca
     4141 tggtgttcaa ggatgtgctg ctgctcggca acgattacat cgtgcccaga cactgtcctg
     4201 agctggccga gatgagccgg gtgtccatca gaatcctgga cgaactggtg ctgcccttcc
     4261 aagagctgca gatcgacgac aacgagtacg cctacctgaa ggccatcatc tttttcgacc
     4321 ccgacgccaa gggcctgagc gatcctggaa agatcaagcg gctgcggagc caggtgcaag
     4381 tgtccctgga agattacatc aacgaccggc agtacgacag ccggggcaga tttggagaac
     4441 tgctgctcct gctgccaaca ctgcagagca tcacctggca gatgatcgag cagatccagt
     4501 tcatcaagct gttcggcatg gccaagatcg acaacctgct gcaagagatg ctgcttggcg
     4561 gcagcccttc tgatgcccct catgctcatc accctctgca ccctcacctg atgcaagaac
     4621 acatgggcac caatgtgatc gtggccaaca caatgcccac acacctgagc aacggccaga
     4681 tgtgcgaatg gcctagacct agaggacagg ccgccacacc tgaaacacct cagccttctc
     4741 cacctggcgg ctctggctct gagccttata agcttctgcc tggcgctgtg gccaccatcg
     4801 tgaaacctct gtctgccatt cctcagccta ccatcaccaa gcaagaagtg atcgaattcg
     4861 gcagcggcga aggcagaggc agccttctta catgtggcga cgtggaagag aaccccggac
     4921 ctatgaatgc ccagctgacc atggaagcca tcggagagct gcacggtgtc tctcacgaac
     4981 ctgttccagc tccagccgat ctgctcggag gatctcctca cgctagaagc agcgtggccc
     5041 acagaggatc tcatctgcct ccagctcacc ccagatccat gggcatggct tctctgctgg
     5101 atggcggatc tggcggcgga gattatcacc accatcacag agcccctgag cactctctgg
     5161 ctggacctct gcaccctacc atgaccatgg cctgtgaaac ccctccaggc atgagcatgc
     5221 ccaccaccta caccacactg acccctctgc aacccctgcc tcctatcagc accgtgtccg
     5281 acaagttccc acaccatcac catcatcacc accaccatca ccatccacat catcaccaga
     5341 gactggccgg caatgtgtcc ggcagcttta ccctgatgag ggacgaacgc ggactggcca
     5401 gcatgaacaa cctgtacacc ccttaccaca aggacgtggc cggcatggga cagtctctgt
     5461 ctccactgtc tagcagcggc ctgggcagca tccacaattc tcagcagggc ctgcctcact
     5521 acgctcatcc tggtgctgcc atgcctaccg acaagatgct gacccctaat ggcttcgagg
     5581 cccaccatcc tgctatgctg ggtagacacg gcgagcagca cctgacacct acatctgctg
     5641 gcatggtgcc catcaacggc ctgccacctc atcaccctca cgctcatctg aacgcccaag
     5701 gacatggaca gctgctggga accgccagag aacccaatcc ttctgtgaca ggcgcccagg
     5761 tgtccaacgg ctctaatagc ggacagatgg aagagatcaa caccaaagag gtggcccagc
     5821 ggatcaccac cgagctgaag agatacagca tccctcaggc catcttcgcc cagagggtgc
     5881 tgtgtagaag ccagggcaca ctgagcgacc tgctgaggaa tcctaagcct tggagcaagc
     5941 tgaagtccgg cagagaaacc ttccggcgga tgtggaagtg gctgcaagag cccgagttcc
     6001 agagaatgag cgccctgaga ctggctgcct gcaagcggaa agagcaagag cacggcaagg
     6061 acaggggcaa cacccctaag aaaccccggc tggtgttcac cgacgtgcag agaagaacac
     6121 tgcacgccat cttcaaagag aacaagcggc ccagcaaaga gctgcagatc acaatcagcc
     6181 agcagctcgg cctggaactg tctaccgtgt ccaacttctt catgaacgcc agacggcgga
     6241 gcctggacaa gtggcaggat gagggcagca gcaacagcgg caatagcagc agcagctcct
     6301 ccacctgtac caaggcttga gctagcaagg atctgcgatc gctccggtgc ccgtcagtgg
     6361 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg
     6421 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     6481 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     6541 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     6601 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     6661 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     6721 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     6781 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     6841 cgttacagat ccaagctgtg accggcgcct acggatccgc catggctaaa cctctgagcc
     6901 aagaggaaag caccctgatc gagagagcca ccgccacaat caatagcatc cccatcagcg
     6961 aggactactc cgtggcttct gctgcactga gcagcgacgg cagaatcttc acaggcgtga
     7021 acgtgtacca cttcaccggc ggaccttgtg ccgaactggt ggttcttgga acagctgccg
     7081 ctgctgcagc cggcaatctg acatgtatcg tggccatcgg caacgagaac cggggcattc
     7141 tcagcccttg tggcagatgc agacaggtcc tgctcgatct gcacccaggc atcaaggcca
     7201 tcgtgaagga ttctgacggc cagcctacag ccgtgggcat tagagaactg ctgcctagcg
     7261 gctatgtgtg ggagggttga aatcaacctc tggattacaa aatttgtgaa agattgactg
     7321 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     7381 atcatgcacg cgtgttaact aaacttgttt attgcagctt ataatggtta caaataaagc
     7441 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7501 tccaaactca tcaatgtatc ttatcatgtc tggaattgac tcaaatgatg tcaattagtc
     7561 tatcagaagc tcatctggtc tcccttccgg gggacaagac atccctgttt aatatttaaa
     7621 cagcagtgtt cccaaactgg gttcttatat cccttgctct ggtcaaccag gttgcagggt
     7681 ttcctgtcct cacaggaacg aagtccctaa agaaacagtg gcagccaggt ttagccccgg
     7741 aattgactgg attccttttt tagggcccat tggtatggct ttttccccgt atccccccag
     7801 gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc cgtgccacct
     7861 tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg gagcgccgga
     7921 ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga cgtaattaca
     7981 tccctggggg ctttgggggg gggctgtccc tgatatctat aacaagaaaa tatatatata
     8041 ataagttatc acgtaagtag aacatgaaat aacaatataa ttatcgtatg agttaaatct
     8101 taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggtcgt tatagttcaa
     8161 aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag cggcgactga
     8221 gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc aatatttcaa
     8281 gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaatctagc tgcatcagga
     8341 tcatatcgtc gggtcttttt tccggctcag tcatcgccca agctggcgct atctgggcat
     8401 cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga aagagtttgc
     8461 cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc acgtttacca tgatgattcg
     8521 ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc gttcaggcca cctgggatac
     8581 cagttcgtcg cggcttttcc ggacacagtt ccggatggtc agcccgaagc gcatcagcaa
     8641 cccgaacaat accggcgaca gccggaactg ccgtgccggt gtgcagatta atgacagcgg
     8701 tgcggcgctg ggatattacg tcagcgagga cgggtatcct ggctggatgc cgcagaaatg
     8761 gacatggata ccccgtgagt tacccggcgg gcgcgcttgg cgtaatcatg gtcatagctg
     8821 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8881 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8941 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     9001 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     9061 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     9121 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9181 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9241 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9301 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9361 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     9421 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9481 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9541 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9601 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9661 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9721 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9781 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9841 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9901 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9961 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10021 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10081 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10141 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10201 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10261 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10321 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10381 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10441 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10501 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10561 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10621 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10681 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10741 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10801 ataagggcga cacggaaatg ttgaatactc at
//
