LOCUS       sequence-405468-        7709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector mediating inducible expression of codon optimized 
            immediate early gene IE180 of pseudorabies virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   Du W, Li E, Guo J, Arano R, Kim Y, Chen YT, Thompson A, Oh SJ, 
            Samuel A, Li Y, Oyibo HK, Xu W
  TITLE     Directed stepwise tracing of polysynaptic neuronal circuits with 
            replication-deficient pseudorabies virus.
  JOURNAL   Cell Rep Methods. 2023 Jun 16;3(6):100506. doi: 
            10.1016/j.crmeth.2023.100506. eCollection 2023 Jun 26.
  PUBMED    37426757
REFERENCE   2  (bases 1 to 7709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2023-06-16"; volume: "3(6):100506. doi"; pages: " 
            10.1016/j.crmeth.2023.100506. eCollection 2023 Jun 26"
COMMENT     Sequence Label: pAAV-TRE-IEo
FEATURES             Location/Qualifiers
     source          1..7709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     69..88
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(105..560)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(242..263)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     454..473
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   635..775
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   646..775
                     /label=AAV2 ITR
     protein_bind    809..827
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    844..862
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    880..898
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    916..934
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    951..969
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    987..1005
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        1017..1055
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1052..1076
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5463..5597
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5500..5519)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5554..5573
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5617..5746
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(5808..6396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5888..5907)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6567..7358,7359..7427))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7190..7209
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7428..7532)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7600..7618
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7656..7678)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
       61 taactatgcg gcatcagagc agattgtact gagagtgcac cataaaattg taaacgttaa
      121 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaatagac
      181 cgaaatcggc aaaatccctt ataaatcaaa agaatagccc gagatagagt tgagtgttgt
      241 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
      301 aaccgtctat cagggcgatg gcccactacg tgaaccatca cccaaatcaa gttttttggg
      361 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
      421 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
      481 taaggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
      541 tgcgccgcta cagggcgcgt actatggttg ctttgacgta tgcggtgtga aataccgcac
      601 agatgcgtaa ggagaaaata ccgcatcagg cgcccctgca ggcagctgcg cgctcgctcg
      661 ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca
      721 gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttcctgcggc
      781 cgcacgcgtg gaggctagac gagtttactc cctatcagtg atagagaacg atgtcgagtt
      841 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
      901 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag
      961 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta
     1021 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcac
     1081 cggaattcgc caccatggct gacgacttgt tcgactttat cgaaaccgag ggaaactttt
     1141 cacagcttct tgcagcagct gccgcagcag cagaggagga aggtatcgca agtggacccg
     1201 acggtggtag tcaggggtca aggcgccgcg gatcttcagg tgaggacctg ctgtttggtc
     1261 ctggaggatt gtttagtgat gatgctgcag aggcagaagc tgaggccgcc gtgctcgccg
     1321 cagccgctgg cgctacacgg cccccccgac ctcccagtgc tcagcaacag cagcctcgcc
     1381 gaggttcagg tgagattgtc gttctcgacg atgaggatga agaagaagat gagcctggca
     1441 gccccgctgc tggaagtcca ggacgagcct tgcatcaagg gtccgaacat gggcatcttg
     1501 tgcttggtcc tagaagccga gcagggagtg gccccagacc acccacaccc gccgctcttg
     1561 ctgccgctga ggcaggtgcc cctgggggcc ccggcaggag cagcccttct gctgcttccc
     1621 cagccagttc cagcggctca cctggcccat ccgccgcccc aaggcgatgg agtcctgccg
     1681 gcgatcctgt tggagaacct ggtccagccg ccagaccaag aacccccgct cctcctgctc
     1741 agcctgccgc agttgctgca gctccagcac gacgcggacc agcctcacca gcacctcctg
     1801 ccgcaggacc agttagcgct ccaggtggag gaggtgcacc cagcgccggt ggagacagag
     1861 gtcgccacca ccaccagcat agggaacctc ttttggacga acccgccgca gccagacggt
     1921 tggaccccag gccacttggt gcaaggtccc ctgtctcatc aaaccccaat tcaaactcct
     1981 ccagcaccac aactgtagca gttgagccag tcgctagggg gcccgagaag gatgaagatg
     2041 gactgggtcc cgccggggat ggaggggcac ctccacaacg acagcccagg cgacggcgag
     2101 ccggcgaagg agcattgaga aggggacgag gatttagcag ttcaagcagc ggagggagcg
     2161 actctgacct gagtcccgct aggtccccaa gcgccccccg cgctctggct gctgctgctg
     2221 ctaggcgaag tgcttccagt agctcaagct cctcatcctc cagttctagc tctagttctt
     2281 cagaaggcga ggaagacgaa ggtgttaggc ctggtgcccc actggcacga gcaggaccac
     2341 caccatctcc ccctgcacct gccgccgcac ctagacctag cgcctcctct gcctctagta
     2401 gtgccgcagc cagccctgcc cccgcacccg aacctgcccg accccctcgc agaaagagac
     2461 gatcaacaaa taaccatctt tcattgatgg ccgatggacc acctccaact gacggaccat
     2521 tgcttacccc actgggtgag ccttggccag ggtctgatcc cccagcagac ggccgcgtcc
     2581 ggtacggggg tgccggcgat tcccgcgaag gactctggga cgaagacgat gtacgccaag
     2641 cagctgcccg ctatcgcgcc gccgcaggac ccgttcccgt gttcattcct gagatgggcg
     2701 attcacggaa acaacacgaa gcccttgtaa gactcattta ctctggtgca gcaggagaag
     2761 caatgtcttg gctccaaaat cccagaatgc aagctcccga ccaacgattc aaccagttct
     2821 gtcaaagaag agtgcatgca ccccatgggc acggaagctt tatcactgga agcgttactc
     2881 cccctctgcc acatataggt gacgctatgg cagcacaaga cccactgtgg gctctgcccc
     2941 atgctgtgtc tgcagttgcc atgtcccgcc ggtatgatcg gactcaaaaa acattcattc
     3001 tgcaatcact tcgccgcgct tacgcagaca tggcatatcc tggtagagcc gcagatcctc
     3061 gggccggtga agccactgta gaggccctgt gcgccagggt tcgggccgct ttcgcagcag
     3121 cccagccagg ccgggttcca cgagaattgg ctgatgcttg cgtacttgca tgccgcggtg
     3181 tactcgaacg cttgcttccc tgccccttgc gcctgccagc acctgccaga gctcctgctg
     3241 ccctggggcc cgcatgtttg gaggaagtca ccgctgctct tctggcactc agagatgcta
     3301 tccccggagc cggccctgca gaacgacaac aagctgcaga cgcagtagca ctcgtagcaa
     3361 gaaccgttgc ccccctggtt cgatattccg tagatggtgc tcgggctcgc gaagctgctt
     3421 ggacctacgc cgctgcattg ttcgcccctg caaatgtcgc tgctgcaaga ctggctgaag
     3481 ctgccgctcg gccaggtccc gccgaacctg cccctggcct ccccccactt tggcccgaac
     3541 aacccgggtt ggtcgtgcca gctcctgcac ctgctgcagc tggcgctccc agtggattgc
     3601 ccggctccgg tcctagttca ccagcctcta ctaaaagttc aagctcaacc gagtccggga
     3661 gcagcaccaa gtcatcttcc gggaccaaat ctggcctgag tggctcttct ggttacgctt
     3721 ccagtcctgc agcagggcca gaccccgccc ctgaacggcg caaaaagaag agacgagcac
     3781 ccggagcacg gcgacccggg gacggtgaag aggatgaagg gctctctggt gccgccctcc
     3841 gcggcgacgg ccacggtcat agggacgatg aggaggatcg cggccctaga cggaaacgga
     3901 gaagtctggg gcttggtcca gcacccgacc ccgcaccagc tctgctcagc agtagcagct
     3961 catccgaaga cgacagattg cgacggcccc tcggacctat gcctgaacat cctgctccag
     4021 acggcggttt ccgaagagta ccagccgggg agactcatac tcctagacct agcgaagccg
     4081 cccttgcagc atattgtccc cctgaggtag ctagagcttt ggtggaccaa gaggtttttc
     4141 ccgagctgtg gcgccctgct cttacatttg accccgctgc cctcgctcac atcgctgctc
     4201 ggcgcggagc tgccggcgct cctttgcgac ggagagctgc atggatgcgc caaatcgccg
     4261 accccgaaga cgttcgagtt gtcgtccttt acgaccccct gccccatgag gaactttgcg
     4321 cagagcctgc tgaaggcgcc ccaaggccag cttgggatcc tagacgaggt ggcctttccg
     4381 ctctgcttgc agcatttgct caccggctgt gcactccaga ttcccacgct tgggcaggaa
     4441 attggactgg gagacccgat atcggtcggt tgaatgccca aggtgtactc ctcttgtctg
     4501 ctcgcgacct cggatttgca ggcgcagtgg agtatctctg ctctcgcctg ggagcagcta
     4561 ggcggaggct tattgttctt gacactattg aagattggcc tgcagacggg cccgccgtcg
     4621 gggattacca tgtgtacgtt cgcgccaggt tggatcccgc cgcccagtgc gctgtacgat
     4681 ggcccggatg cagggaactt agggctgccg tgttggactc tagttcaatc gttggtcctg
     4741 catgctttgc tcgagcagag gcttcatttg caaggttgca ccccggcgcc gaacccttga
     4801 gactctgccg acaggacaat gtgcggtaca ctgtatctac acgagcaggg ccacggacac
     4861 ctgttcctct tcccccaaga gcataccgcc aacgggtact ccctacagta gacggatgta
     4921 aggacatggc tcgccagcgc tcagctttgg gactcggcga ccccgacttc gacgctggag
     4981 cagccttcgg tcatcgggca gccaacaggt gggggctcgg ggccccattg cgccctgtct
     5041 tcgtgtcctg tggaagaagg gggctggcag aattgcgcgg cccagagggc cttccagccg
     5101 agttgcgggc cttttgtgcc gctgccctcc ttgagcctga tgccgaagca gctcccttgg
     5161 tgctcacccc cggggccgta gctgcagccg gtgcacctcc agctgtcttg tgggattttg
     5221 ccccatttga aacttccgtt cgagcagcag ctgggggagc cgtggaaact cgacgccccg
     5281 caggggcttc aggggccggc gccggaccag gagaagatgg tgattctgtt gagattgtcg
     5341 gggtgcgggg tggggacgga cgaccacggg ggcccctcgg accaattaag gttgaggcaa
     5401 tctctgacga tgaagaagca gaagatgctg gcaaccctta ccttcttctg cgctaatcta
     5461 gaaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5521 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5581 cttatcatgt ctggatcgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     5641 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     5701 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggacat gtgagcaaaa
     5761 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5821 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5881 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5941 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6001 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6061 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6121 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6181 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6241 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6301 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6361 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6421 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6481 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6541 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6601 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6661 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6721 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6781 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6841 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6901 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     6961 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7021 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7081 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7141 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7201 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7261 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7321 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7381 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7441 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7501 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     7561 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     7621 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     7681 acggtcacag cttgtctgta agcggatgc
//
