LOCUS       sequence-405487-       19931 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prime Editing plasmid for tag insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19931)
  AUTHORS   Li J, Ding J, Zhu J, Xu R, Gu D, Liu X, Liang J, Qiu C, Wang H, Li 
            M, Qin R, Wei P
  TITLE     Prime editing-mediated precise knockin of protein tag sequences in 
            the rice genome.
  JOURNAL   Plant Commun. 2023 Mar 7:100572. doi: 10.1016/j.xplc.2023.100572.
  PUBMED    36883004
REFERENCE   2  (bases 1 to 19931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2023 Mar 7:100572. doi: 10.1016/j.xplc.2023.100572."
COMMENT     Sequence Label: ePE2
FEATURES             Location/Qualifiers
     source          1..19931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     233..255
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     247..264
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     248..264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1623..2414
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     ncRNA           2566..2633
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     terminator      2664..2912
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     regulatory      4904..4913
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4916..4936
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9311..9331
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10901..10921
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10931..10957
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     protein_bind    complement(10983..11007)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      11020..11029
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(11306..11322)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11306..11322)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11319..11341)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11330..11346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11354..11360,11361..11378,11379..11384))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11399..11420
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11611..12287
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     12224..12244
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             12354..13379
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    13420..13594
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    13672..13696
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             14121..14915
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      15002..15590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15491..15510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15744..15761
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15776..15916
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15842..15861)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     16002..16024
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      16260..16454
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16520..17593)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(18022..18651)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
      121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      241 cgacgttgta aaacgacggc cagtgccaag cttatggagt caaagattca aatagaggac
      301 ctaacagaac tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat
      361 gacaagaaga aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat
      421 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
      481 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
      541 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
      601 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      661 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgattg gcagacatac
      721 tgtcccacaa atgaagatgg aatctgtaaa agaaaacgcg tgaaataatg cgtctgacaa
      781 aggttaggtc ggctgccttt aatcaatacc aaagtggtcc ctaccacgat ggaaaaactg
      841 tgcagtcggt ttggcttttt ctgacgaaca aataagattc gtggccgaca ggtgggggtc
      901 caccatgtga aggcatcttc agactccaat aatggagcaa tgacgtaagg gcttacgaaa
      961 taagtaaggg tagtttggga aatgtccact cacccgtcag tctataaata cttagcccct
     1021 ccctcattgt taagggagca aaatctcaga gagatagtcc tagagagaga aagagagcaa
     1081 gtagcctaga agtagtcaag gcggcgaagt attcaggcac gtggccagga agaagaaaag
     1141 ccaagacgac gaaaacaggt aagagctaag catctagaaa gttgaaaaca atcttcaaaa
     1201 gtcccacatc gcttagataa gaaaacgaag ctgagtttat atacagctag agtcgaagta
     1261 gtgattgaac aaagcaccag tggtctagtg gtagaatagt accctgccac ggtacagacc
     1321 cgggttcgat tcccggctgg tgcaagagac caacccagtg gacataagcc tgttcggttc
     1381 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     1441 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     1501 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     1561 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     1621 tcatggggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     1681 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     1741 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     1801 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     1861 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     1921 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     1981 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2041 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2101 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2161 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2221 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     2281 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     2341 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     2401 tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg
     2461 cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca
     2521 agtctgcttt tattattttt aagcgtgcat aataagccgg tctcaggccg gcatggtccc
     2581 agcctcctcg ctggcgccgg ctgggcaaca tgcttcggca tggcgaatgg gacttttttt
     2641 tgatatctcc ggggctaatt gaatatgaag atgaagatga aatatttggt gtgtcaaata
     2701 aaaagctggt gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg
     2761 ctttttctaa tattaaatga atgtaagatc tcattataat gaataaacaa atgtttctat
     2821 aatccattgt gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat
     2881 ggtatggact atggaatatg attaaagata agaagctttg cagcgtgacc cggtcgtgcc
     2941 cctctctaga gataatgagc attgcatgtc taagttataa aaaattacca catatttttt
     3001 ttgtcacact tgtttgaagt gcagtttatc tatctttata catatattta aactttactc
     3061 tacgaataat ataatctata gtactacaat aatatcagtg ttttagagaa tcatataaat
     3121 gaacagttag acatggtcta aaggacaatt gagtattttg acaacaggac tctacagttt
     3181 tatcttttta gtgtgcatgt gttctccttt ttttttgcaa atagcttcac ctatataata
     3241 cttcatccat tttattagta catccattta gggtttaggg ttaatggttt ttatagacta
     3301 atttttttag tacatctatt ttattctatt ttagcctcta aattaagaaa actaaaactc
     3361 tattttagtt tttttattta ataatttaga tataaaatag aataaaataa agtgactaaa
     3421 aattaaacaa atacccttta agaaattaaa aaaactaagg aaacattttt cttgtttcga
     3481 gtagataatg ccagcctgtt aaacgccgtc gacgagtcta acggacacca accagcgaac
     3541 cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg catctctgtc gctgcctctg
     3601 gacccctctc gagagttccg ctccaccgtt ggacttgctc cgctgtcggc atccagaaat
     3661 tgcgtggcgg agcggcagac gtgagccggc acggcaggcg gcctcctcct cctctcacgg
     3721 caccggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc
     3781 cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc
     3841 acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg
     3901 ccgctcgtcc tccccccccc cctctctacc ttctctagat cggcgttccg gtccatggtt
     3961 agggcccggt agttctactt ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc
     4021 gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca gacacgttct gattgctaac
     4081 ttgccagtgt ttctctttgg ggaatcctgg gatggctcta gccgttccgc agacgggatc
     4141 gatttcatga ttttttttgt ttcgttgcat agggtttggt ttgccctttt cctttatttc
     4201 aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat gctttttttt gtcttggttg
     4261 tgatgatgtg gtctggttgg gcggtcgttc tagatcggag tagaattctg tttcaaacta
     4321 cctggtggat ttattaattt tggatctgta tgtgtgtgcc atacatattc atagttacga
     4381 attgaagatg atggatggaa atatcgatct aggataggta tacatgttga tgcgggtttt
     4441 actgatgcat atacagagat gctttttgtt cgcttggttg tgatgatgtg gtgtggttgg
     4501 gcggtcgttc attcgttcta gatcggagta gaatactgtt tcaaactacc tggtgtattt
     4561 attaattttg gaactgtatg tgtgtgtcat acatcttcat agttacgagt ttaagatgga
     4621 tggaaatatc gatctaggat aggtatacat gttgatgtgg gttttactga tgcatataca
     4681 tgatggcata tgcagcatct attcatatgc tctaaccttg agtacctatc tattataata
     4741 aacaagtatg ttttataatt attttgatct tgatatactt ggatgatggc atatgcagca
     4801 gctatatgtg gattttttta gccctgcctt catacgctat ttatttgctt ggtactgttt
     4861 cttttgtcga tgctcaccct gttgtttggt gttacttctg caggccacca tggccccaaa
     4921 gaagaagcgc aaggtcgaca agaagtactc catcggcctc gacatcggca ccaattctgt
     4981 tggctgggcc gtgatcaccg acgagtacaa ggtgccgtcc aagaagttca aggtcctcgg
     5041 caacaccgac cgccactcca tcaagaagaa tctcatcggc gccctgctgt tcgactctgg
     5101 cgagacagcc gaggctacaa ggctcaagag gaccgctaga cgcaggtaca ccaggcgcaa
     5161 gaaccgcatc tgctacctcc aagagatctt ctccaacgag atggccaagg tggacgacag
     5221 cttcttccac aggctcgagg agagcttcct cgtcgaggag gacaagaagc acgagcgcca
     5281 tccgatcttc ggcaacatcg tggatgaggt ggcctaccac gagaagtacc cgaccatcta
     5341 ccacctccgc aagaagctcg tcgactccac cgataaggcc gacctcaggc tcatctacct
     5401 cgccctcgcc cacatgatca agttcagggg ccacttcctc atcgagggcg acctcaaccc
     5461 ggacaactcc gatgtggaca agctgttcat ccagctcgtg cagacctaca accagctgtt
     5521 cgaggagaac ccgatcaacg cctctggcgt tgacgccaag gctattctct ctgccaggct
     5581 ctctaagtcc cgcaagctcg agaatctgat cgcccaactt ccgggcgaga agaagaatgg
     5641 cctcttcggc aacctgatcg ccctctctct tggcctcacc ccgaacttca agtccaactt
     5701 cgacctcgcc gaggacgcca agctccagct ttccaaggac acctacgacg acgacctcga
     5761 caatctcctc gcccagattg gcgatcagta cgccgatctg ttcctcgccg ccaagaatct
     5821 ctccgacgcc atcctcctca gcgacatcct cagggtgaac accgagatca ccaaggcccc
     5881 actctccgcc tccatgatca agaggtacga cgagcaccac caggacctca cactcctcaa
     5941 ggccctcgtg agacagcagc tcccagagaa gtacaaggag atcttcttcg accagtccaa
     6001 gaacggctac gccggctaca tcgatggcgg cgcttctcaa gaggagttct acaagttcat
     6061 caagccgatc ctcgagaaga tggacggcac cgaggagctg ctcgtgaagc tcaagagaga
     6121 ggacctcctc cgcaagcagc gcaccttcga taatggctcc atcccgcacc agatccacct
     6181 cggcgagctt catgctatcc tccgcaggca agaggacttc tacccgttcc tcaaggacaa
     6241 ccgcgagaag attgagaaga tcctcacctt ccgcatcccg tactacgtgg gcccgctcgc
     6301 caggggcaac tccaggttcg cctggatgac cagaaagtcc gaggagacaa tcaccccctg
     6361 gaacttcgag gaggtggtgg ataagggcgc ctctgcccag tctttcatcg agcgcatgac
     6421 caacttcgac aagaacctcc cgaacgagaa ggtgctcccg aagcactcac tcctctacga
     6481 gtacttcacc gtgtacaacg agctgaccaa ggtgaagtac gtgaccgagg ggatgaggaa
     6541 gccagctttc cttagcggcg agcaaaagaa ggccatcgtc gacctgctgt tcaagaccaa
     6601 ccgcaaggtg accgtgaagc agctcaagga ggactacttc aagaaaatcg agtgcttcga
     6661 ctccgtcgag atctccggcg tcgaggatag gttcaatgcc tccctcggga cctaccacga
     6721 cctcctcaag attatcaagg acaaggactt cctcgacaac gaggagaacg aggacatcct
     6781 cgaggacatc gtgctcaccc tcaccctctt cgaggaccgc gagatgatcg aggagcgcct
     6841 caagacatac gcccacctct tcgacgacaa ggtgatgaag cagctgaagc gcaggcgcta
     6901 taccggctgg ggcaggctct ctaggaagct catcaacggc atccgcgaca agcagtccgg
     6961 caagacgatc ctcgacttcc tcaagtccga cggcttcgcc aaccgcaact tcatgcagct
     7021 catccacgac gactccctca ccttcaagga ggacatccaa aaggcccagg tgtccggcca
     7081 aggcgattcc ctccatgaac atatcgccaa tctcgccggc tccccggcta tcaagaaggg
     7141 cattctccag accgtgaagg tggtggacga gctggtgaag gtgatgggca ggcacaagcc
     7201 agagaacatc gtgatcgaga tggcccgcga gaaccagacc acacagaagg gccaaaagaa
     7261 ctcccgcgag cgcatgaaga ggatcgagga gggcattaag gagctgggct cccagatcct
     7321 caaggagcac ccagtcgaga acacccagct ccagaacgag aagctctacc tctactacct
     7381 ccagaacggc cgcgacatgt acgtggacca agagctggac atcaaccgcc tctccgacta
     7441 cgacgtggac gctattgtgc cgcagtcctt cctgaaggac gactccatcg acaacaaggt
     7501 gctcacccgc tccgacaaga acaggggcaa gtccgataac gtgccgtccg aagaggtcgt
     7561 caagaagatg aagaactact ggcgccagct cctcaacgcc aagctcatca cccagaggaa
     7621 gttcgacaac ctcaccaagg ccgagagagg cggcctttcc gagcttgata aggccggctt
     7681 catcaagcgc cagctcgtcg agacacgcca gatcacaaag cacgtggccc agatcctcga
     7741 ctcccgcatg aacaccaagt acgacgagaa cgacaagctc atccgcgagg tgaaggtcat
     7801 caccctcaag tccaagctcg tgtccgactt ccgcaaggac ttccagttct acaaggtgcg
     7861 cgagatcaac aactaccacc acgcccacga cgcctacctc aatgccgtgg tgggcacagc
     7921 cctcatcaag aagtacccaa agctcgagtc cgagttcgtg tacggcgact acaaggtgta
     7981 cgacgtgcgc aagatgatcg ccaagtccga gcaagagatc ggcaaggcga ccgccaagta
     8041 cttcttctac tccaacatca tgaatttctt caagaccgag atcacgctcg ccaacggcga
     8101 gattaggaag aggccgctca tcgagacaaa cggcgagaca ggcgagatcg tgtgggacaa
     8161 gggcagggat ttcgccacag tgcgcaaggt gctctccatg ccgcaagtga acatcgtgaa
     8221 gaagaccgag gttcagaccg gcggcttctc caaggagtcc atcctcccaa agcgcaactc
     8281 cgacaagctg atcgcccgca agaaggactg ggacccgaag aagtatggcg gcttcgattc
     8341 tccgaccgtg gcctactctg tgctcgtggt tgccaaggtc gagaagggca agagcaagaa
     8401 gctcaagtcc gtcaaggagc tgctgggcat cacgatcatg gagcgcagca gcttcgagaa
     8461 gaacccaatc gacttcctcg aggccaaggg ctacaaggag gtgaagaagg acctcatcat
     8521 caagctcccg aagtacagcc tcttcgagct tgagaacggc cgcaagagaa tgctcgcctc
     8581 tgctggcgag cttcagaagg gcaacgagct tgctctcccg tccaagtacg tgaacttcct
     8641 ctacctcgcc tcccactacg agaagctcaa gggctcccca gaggacaacg agcaaaagca
     8701 gctgttcgtc gagcagcaca agcactacct cgacgagatc atcgagcaga tctccgagtt
     8761 ctccaagcgc gtgatcctcg ccgatgccaa cctcgataag gtgctcagcg cctacaacaa
     8821 gcaccgcgat aagccaattc gcgagcaggc cgagaacatc atccacctct tcaccctcac
     8881 caacctcggc gctccagccg ccttcaagta cttcgacacc accatcgacc gcaagcgcta
     8941 cacctctacc aaggaggttc tcgacgccac cctcatccac cagtctatca caggcctcta
     9001 cgagacacgc atcgacctct cacaactcgg cggcgatagt ggttctgaaa ctcctggaac
     9061 atcagaatcg gcgacgccgg agagcgctac cgtcgtgtcc gggcagaaac aagatagaca
     9121 aggcggcgag aggaggcgta gccagctgga ccgcgaccag tgcgcctact gcaaggagaa
     9181 gggccactgg gcaaaagatt gtccaaagaa gccccgcggg ccaagaggac ctcggccgca
     9241 gacctccctc ctttcaggcg gctccagcgg cggctctaag cggaccgccg acggatcaga
     9301 gttcgagagc ccgaagaaga agaggaaggt gtccggcggc tcatctggcg gctccacact
     9361 caatatcgag gacgagtaca ggctgcatga gacatccaag gagcctgacg tctccctcgg
     9421 cagcacatgg ctctcagatt tcccacaggc ctgggccgag acaggcggca tgggcctcgc
     9481 cgtccgccag gcgccgctca tcattccact gaaggcgacc tccacaccgg tgagcatcaa
     9541 gcagtaccca atgtctcagg aggcaaggct tggcatcaag ccacacattc agaggctcct
     9601 ggaccagggc attctggtgc cttgccagag cccgtggaac acccctctcc tgccggtgaa
     9661 gaagcctggc acaaatgact accgcccggt ccaggatctc agggaggtga acaagcgcgt
     9721 cgaggatatc catccgacag tcccgaaccc atacaatctc ctgtcaggcc tcccgccatc
     9781 tcaccagtgg tacaccgtgc tcgacctgaa ggatgcgttc ttctgcctca ggctgcatcc
     9841 aacaagccag cctctcttcg ccttcgagtg gcgcgatccg gagatgggca tttcaggcca
     9901 gctcacctgg acacggctgc cacagggctt caagaactct cctaccctct tcaatgaggc
     9961 gctccatcgg gacctggccg atttcaggat ccagcaccca gacctcattc tcctccagta
    10021 tgtggacgat ctcctgctcg ccgcgacatc cgagctggat tgccagcagg gaacccgcgc
    10081 gctgctccag acactgggaa atctgggata cagggcatca gcgaagaagg cacagatctg
    10141 ccagaagcag gtcaagtacc tcggctacct gctcaaggag ggacagaggt ggctgacaga
    10201 ggcaaggaag gagacagtga tgggccagcc taccccgaag acaccacggc agctcaggga
    10261 gttcctgggc aaggcgggct tctgccgcct cttcatccca ggattcgcgg agatggcggc
    10321 gccactctac cctctgacca agcctggcac actgttcaac tggggaccag accagcagaa
    10381 ggcgtaccag gagattaagc aggccctgct cacagcacct gccctcggcc tgccggacct
    10441 cacaaagcca ttcgagctgt tcgtggatga gaagcagggc tacgcgaagg gagtcctgac
    10501 acagaagctg ggaccatgga ggcgcccagt ggcctacctc tccaagaagc tggacccagt
    10561 ggctgccggc tggcctccgt gcctgaggat ggtggcggcc attgccgtcc tcaccaagga
    10621 tgccggcaag ctgacaatgg gccagcctct cgtcattctg gcgccgcatg cggtggaggc
    10681 gctcgtcaag cagccacctg ataggtggct gtccaacgcg cgcatgaccc actaccaggc
    10741 cctgctcctg gacacagata gggtgcagtt cggcccagtg gtcgccctca atcctgccac
    10801 actgctgcca ctccctgagg agggcctcca gcataactgc ctcgatattc tggcggaggc
    10861 ccatggaacc cgccctgacc tcacagatca gccgctgagc ccgaagaaga agaggaaagt
    10921 gggatcagga ccagccgcca agagggtgaa gctcgattga gagctcaagg gtgggcgcgc
    10981 cgacccagct ttcttgtaca aagtggtgat atcccgcggc catggcggcc gggagcatgc
    11041 gacgtcgatc taactgacta gccgcggcca tgctagagtc cgcaaaaatc accagtctct
    11101 ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag ttcccagata
    11161 agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt
    11221 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
    11281 ccagtgacct ggaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc
    11341 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    11401 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    11461 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    11521 gctagagcag cttgccaaca tggtggagca cgacactctc gtctactcca agaatatcaa
    11581 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg
    11641 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa
    11701 ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
    11761 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
    11821 agacgttcca accacgtctt caaagcaagt ggattgatgt gaacatggtg gagcacgaca
    11881 ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga
    11941 cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc
    12001 acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata
    12061 aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
    12121 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
    12181 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
    12241 cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaaat caccagtctc
    12301 tctctacaaa tctatctctc tcgagctttc gcagatcccg ggggcaatga gatatgaaaa
    12361 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
    12421 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
    12481 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
    12541 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggagt
    12601 ttagcgagag cctgacctat tgcatctccc gccgttcaca gggtgtcacg ttgcaagacc
    12661 tgcctgaaac cgaactgccc gctgttctac aaccggtcgc ggaggctatg gatgcgatcg
    12721 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
    12781 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
    12841 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
    12901 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
    12961 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
    13021 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
    13081 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccacgactcc
    13141 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
    13201 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
    13261 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
    13321 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aagaaataga
    13381 gtagatgccg accgggatct gtcgatcgac aagctcgagt ttctccataa taatgtgtga
    13441 gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat
    13501 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
    13561 cctaaaacca aaatccagta ctaaaatcca gatcccccga attaattcgg cgttaattca
    13621 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
    13681 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
    13741 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
    13801 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
    13861 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
    13921 gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac
    13981 tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac
    14041 agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga
    14101 aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga
    14161 tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc
    14221 tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca
    14281 cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc
    14341 caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg
    14401 atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc
    14461 tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga
    14521 atagcttaga cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg
    14581 tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt
    14641 ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca
    14701 gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca
    14761 gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg
    14821 aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa
    14881 taaaatatta tattttactg gatgaattgt tttagtacct agaatgcatg accaaaatcc
    14941 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    15001 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    15061 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    15121 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
    15181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    15241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    15301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    15361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    15421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    15481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    15541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    15601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    15661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    15721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggat gagcgcctga
    15781 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
    15841 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
    15901 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
    15961 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
    16021 gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc
    16081 ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag
    16141 ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc
    16201 gcagcgaccg aagggtaggc gctttttgca gcacttcggc tgtgcgctgg ccagacagtt
    16261 atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa
    16321 aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg
    16381 gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt
    16441 acgtgctatc cacaggaaac agaccttttc gacctttttc ccctgctagg gcaatttgcc
    16501 ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt
    16561 agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca
    16621 ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc
    16681 tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc
    16741 ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt
    16801 gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta
    16861 gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa
    16921 tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct
    16981 gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct
    17041 ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc
    17101 ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa
    17161 ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa
    17221 cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct
    17281 tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat
    17341 agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg
    17401 gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc
    17461 acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct
    17521 tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt
    17581 tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca
    17641 ggcaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac
    17701 ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca
    17761 tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca
    17821 gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg
    17881 ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg
    17941 cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca
    18001 ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg
    18061 gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc
    18121 agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc
    18181 ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac
    18241 aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag
    18301 gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc
    18361 tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa
    18421 gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac
    18481 ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga
    18541 atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat
    18601 ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc
    18661 ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg
    18721 ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag
    18781 ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc
    18841 acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg
    18901 atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt
    18961 cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac
    19021 ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc
    19081 ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt
    19141 cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc
    19201 caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta
    19261 gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg
    19321 tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc
    19381 gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg
    19441 tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc
    19501 tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc
    19561 gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg
    19621 cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc
    19681 gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa
    19741 cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga
    19801 ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg gaaccctgtg
    19861 gttggcatgc acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc
    19921 gattattcta a
//
