LOCUS       sequence-405488-       15316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas9 under UAS control. HDR integrant markers with DsRed 
            expressed in flight muscles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15316)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 15316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT57
FEATURES             Location/Qualifiers
     source          1..15316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             411..1202
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1448..2036
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1937..1956
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2190..2207
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2239..2257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2239..2256
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        2334..2754
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     protein_bind    2761..2884
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2907..2912,2913..2930,2931..2937)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        2980..3084
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3085..3153,3154..3945)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3303..3322)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     4466..4485
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4625..4748)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    4952..5046
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5080..5174
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5205..5443
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     5390..5411
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          5458..5524
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             5557..5577
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5578..7293
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     RBS             9135..9143
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9664..9684
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10443..10577
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10467..10486)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10521..10540
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(10685..11359,11360..11362,11363..11365))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(10747..10770)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(10945..10964)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     11162..11182
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     regulatory      11362..11371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    12236..12359
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(12382..12387,12388..12405,12406..12412)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             12466..13125
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(12532..12551)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        13134..13579
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(13580..13666,13667..15001)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     protein_bind    complement(15040..15163)
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(15255..15274)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(15256..15274)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
       61 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
      121 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
      181 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
      241 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
      301 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      361 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
      421 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
      481 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
      541 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
      601 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
      661 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
      721 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
      781 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
      841 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
      901 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
      961 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     1021 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1081 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1141 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     1201 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     1261 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     1321 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca
     1381 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1441 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1501 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1561 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1621 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1681 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1741 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1801 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1861 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1921 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1981 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2041 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2101 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2161 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa atcgtcacct
     2221 gcaggaaggt ttaaacgcat ttaggtgaca ctatagaagt gtgtatcgct cgagggatcc
     2281 gaattcgaag tcttggtacg gaggggatcc gatcgacaag ccgaacatat gggttttttg
     2341 ctcacctgtg attgctccta ctcaaataca aaaacatcaa attttctgtc aataaagcat
     2401 atttatttat atttatttta caggaaagaa ttccttttaa agtgtatttt aacctataat
     2461 gaaaaacgat taaaaaaaat acataaaata attcgaaaat ttttgaatag cccaggttga
     2521 taaaaattca tttcatacgt tttataactt atgcccctaa gtattttttg accatagtgt
     2581 ttcaattcta cattaatttt acagagtaga atgaaacgcc acctactcag ccaagaggcg
     2641 aaaaggttag ctcgccaagc agagagggcg ccagtgctca ctacttttta taattctcaa
     2701 cttctttttc cagactcagt tcgtatatat agacctattt tcaatttaac gtcggggcct
     2761 caactttgta taataaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     2821 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     2881 tatgggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
     2941 ggattttgag ttaggatccg tcgagatttt caggagctac gcggaacccc tatttgttta
     3001 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3061 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3121 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3181 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3241 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3301 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3361 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3421 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3481 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3541 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3601 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3661 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3721 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3781 tctggagccg gtgagcgtgg gtcccgcggt atcattgcag cactggggcc agatggtaag
     3841 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3901 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaatggat ccggcttact
     3961 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     4021 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     4081 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     4141 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     4201 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     4261 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     4321 tatcgtctgt ttgtggatgt acagagtgat attatcgata caccgggccg tcgcatggtt
     4381 attccacttg cgtctgcgcg cttgctctca gataaagtct cccgtgaact ttacccggtg
     4441 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtt
     4501 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     4561 attaacctga tgttctgggg aatataatgt caggctccct tatacacagc cagtctgcac
     4621 gtcgcatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     4681 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa cttttctata
     4741 caaagttgtc cagctccgag ctcggtacct cgcatgttgt tgctcaggtc gcagacgttt
     4801 tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa
     4861 ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg
     4921 gccagggccg caagcttgca tgcctgcagg tcggagtact gtcctccgag cggagtactg
     4981 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
     5041 tccgagcgga gactctagcc ctagggcatg cctgcaggtc ggagtactgt cctccgagcg
     5101 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
     5161 tactgtcctc cgagcggaga ctctagcgct agcgacgtcg agcgccggag tataaataga
     5221 ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag
     5281 cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatctgc agtaaagtgc
     5341 aagttaaagt gaatcaatta aaagtaacca gcaaccaagt aaatcaactg caactactga
     5401 aatctgccaa gaagtaatta ttgaatacaa gaagagaact ctgaatagat ctaaaaggta
     5461 ggttcaacca ctgatgccta ggcacaccga aacgactaac cctaattctt atcctttact
     5521 tcaggcggcc gcggctcgag ggtaccaatc aaaatgccca aaaagaaacg caaggtggac
     5581 aagaaatact ccatcggact ggacatcggg acgaactcag tgggttgggc cgtgatcacc
     5641 gatgaataca aggtgcccag caaaaagttc aaggtgctgg gcaacaccga ccgccactcc
     5701 atcaaaaaga acctcattgg cgctctcctg ttcgacagcg gcgaaactgc tgaggccaca
     5761 cgcctgaagc gcaccgcacg cagaaggtac accaggagaa aaaaccgcat ctgctatctg
     5821 caggaaatct tttccaatga gatggctaag gtggatgaca gctttttcca ccgcctcgaa
     5881 gagtcttttc tggtggaaga ggacaaaaag cacgagcgcc accctatctt tggtaacatt
     5941 gtggacgagg tggcgtatca cgagaagtat cctaccattt accaccttcg caaaaagctg
     6001 gtcgatagca ctgataaggc cgatctgcgc ctcatctacc tggccttggc ccatatgatc
     6061 aagttccgtg ggcacttcct catcgaaggt gatctgaacc ctgacaatag cgacgtggat
     6121 aagctgttca tccagctggt ccagacttac aaccagctgt tcgaggaaaa ccccatcaat
     6181 gcttccgggg tagatgctaa ggctatcctg agtgctaggc tgtctaagtc tcggcgtctg
     6241 gagaatctga ttgcgcagct gcctggtgag aaaaagaatg gactgttcgg caacctgatc
     6301 gccctgtccc tgggccttac cccgaacttc aagtctaact ttgacctcgc tgaggacgcc
     6361 aaacttcagc tgagcaagga cacatacgac gatgaccttg acaacctgct cgcccagatc
     6421 ggtgaccagt acgccgatct gttcctggca gccaagaacc tgtctgacgc aatcttgctc
     6481 agcgatatcc tgcgcgtcaa caccgagatc accaaggcac ctctgagcgc aagcatgatt
     6541 aaacgctatg acgaacatca ccaggacctc acgctcctga aagccctggt ccgccaacag
     6601 ctgcccgaga aatacaagga gattttcttt gatcagtcca agaacgggta cgctggctat
     6661 atcgatggcg gagcgagcca ggaagagttc tacaagttta tcaagccgat cctggagaaa
     6721 atggacggca ccgaagagct cctggtgaag ctgaacaggg aggaccttct gcgcaaacag
     6781 cggacctttg ataatgggag catcccccac cagattcatt tgggtgagct gcacgccatc
     6841 ctgcgccgtc aagaggactt ctaccccttc ctcaaagata accgcgaaaa gattgaaaag
     6901 attctgacct ttcgcatccc atattacgtg ggccccctcg ccagaggcaa cagccgcttt
     6961 gcatggatga cgcgcaagtc agaagagacc attaccccct ggaactttga agaggtcgtt
     7021 gacaaaggtg catcagctca gagctttatc gagcggatga ctaacttcga taagaacctg
     7081 ccaaacgaga aggtactgcc taagcattct ctcctgtacg aatactttac cgtgtataat
     7141 gagctgacga aggtgaaata cgtcactgag ggtatgcgca agcctgcgtt ccttagcgga
     7201 gagcagaaaa aggctattgt ggatctcttg tttaagacaa acaggaaggt taccgtgaag
     7261 caactgaagg aggattattt caaaaagatt gagtgtttcg actctgtgga aatctccggc
     7321 gtcgaggacc ggttcaacgc atctttgggc acgtatcatg acctcctgaa gattatcaag
     7381 gacaaagact ttctggataa tgaagagaac gaagatatcc tcgaggacat cgtgctgact
     7441 ctcactctgt ttgaggaccg cgaaatgatc gaagagcgcc tcaagactta tgcccacctt
     7501 ttcgatgaca aagtgatgaa gcagctcaaa cgtcggcgtt ataccggatg gggacgcctg
     7561 tctcgtaagc tgatcaacgg catccgggac aagcaatccg gcaagaccat tctggacttc
     7621 cttaagagcg atggtttcgc caatagaaat ttcatgcaac tcatccacga tgacagtctt
     7681 acattcaagg aggacatcca gaaggcccag gtgagcggac agggcgacag ccttcatgag
     7741 cacatcgcta acctggcagg atcccccgcc atcaaaaagg gcatcctgca gaccgtcaag
     7801 gttgtggacg agttggtgaa agtgatgggc cgccacaagc ctgagaatat cgtgattgag
     7861 atggcgcgtg aaaaccagac tacccaaaag ggacagaaga acagtcgcga acgcatgaag
     7921 cgcatcgaag agggtattaa ggagctgggt agtcagattc tgaaggagca ccctgtggag
     7981 aacacccagc tccagaacga gaaactctac ctgtactatc tgcagaacgg acgcgatatg
     8041 tacgtggacc aggagctcga catcaacagg cttagcgact acgacgtaga tcatatcgtg
     8101 cctcagtctt tcctgaaaga tgactctatc gacaacaagg tgctgacacg ctctgataag
     8161 aatcgtggca agtccgacaa cgttccttct gaagaggtgg tcaagaaaat gaagaattac
     8221 tggaggcagc tcctgaacgc taaactgatc actcaaagaa aattcgacaa cctgaccaag
     8281 gctgaacgcg gtggcctgag cgagctggat aaagcgggtt tcatcaagag acagctggtc
     8341 gaaacgcgtc aaatcacaaa gcacgtagct cagatcctgg attccaggat gaacaccaaa
     8401 tacgacgaaa acgataagct gattcgcgag gtgaaggtga ttacccttaa gtctaagctg
     8461 gtgagcgatt ttcgcaaaga ctttcagttt tataaagtcc gtgagatcaa caattatcat
     8521 cacgctcatg atgcctatct gaatgctgtg gtcggaactg ctctgattaa aaagtaccct
     8581 aagctcgaga gcgaattcgt atatggagac tacaaagtgt atgatgtgag gaagatgatc
     8641 gccaagtccg agcaggaaat cggaaaggcg accgccaagt actttttcta tagcaacatc
     8701 atgaattttt tcaaaaccga gattaccttg gctaacggtg agatccgcaa gcgtcccctc
     8761 atcgaaacga acggcgaaac gggtgaaatc gtttgggata aaggtcggga cttcgccaca
     8821 gtgcgcaagg tgctctcaat gccacaggtg aacatcgtga aaaagaccga ggtgcaaact
     8881 ggcggtttca gcaaagagtc catcctcccg aaacgcaact ccgacaagct gattgcccgg
     8941 aaaaaggatt gggatcccaa aaagtatggt ggcttcgact ctccaacagt ggcttattct
     9001 gtgttggttg tcgcgaaggt ggagaagggc aaaagtaaga aactgaagtc cgtgaaggag
     9061 ctcctgggta tcaccatcat ggagagatct tccttcgaga agaaccccat cgatttcctc
     9121 gaggccaagg gctataagga ggtgaaaaag gatttgatca ttaagcttcc caagtacagc
     9181 ctgttcgagc tggagaacgg ccgcaagcgc atgctcgcta gtgctggtga gctgcagaag
     9241 ggaaacgagc tggcactccc aagtaagtat gtgaactttc tgtatctggc tagccattat
     9301 gagaagctca aaggtagccc agaggacaat gagcagaaac agctttttgt ggaacagcac
     9361 aagcactatt tggacgaaat tatcgagcag atcagcgagt ttagcaagag ggtaatcctg
     9421 gccgatgcta acctggacaa ggtcctgagt gcttataaca agcaccgcga caagccgatc
     9481 cgtgaacagg ccgagaatat tatccacctc ttcactctta cgaatctggg cgctccagct
     9541 gccttcaagt acttcgatac gactattgac agaaaaaggt atacctccac gaaggaagtg
     9601 ctggatgcca ccctgatcca ccaatccatc actggtttgt atgaaacgcg catcgacctg
     9661 tcacccaaga aaaagcgcaa ggtgcagctg ggcggggact agtctagaat gaatcgtttt
     9721 taaaataaca aatcaattgt tttataatat tcgtacgatt ctttgattat gtaataaaat
     9781 gtgatcatta ggaagattac gaaaaatata aaaaatatga gttctgtgtg tataacaaat
     9841 gctgtaaacg ccacaattgt gtttgttgca aataaaccca tgattatttg attaaaattg
     9901 ttgttttctt tgttcataga caatagtgtg ttttgcctaa acgtgtactg cataaactcc
     9961 atgcgagtgt atagcgagct agtggctaac gcttgcccca ccaaagtaga ttcgtcaaaa
    10021 tcctcaattt catcaccctc ctccaagttt aacatttggc cgtcggaatt aacttctaaa
    10081 gatgccacat aatctaataa atgaaataga gattcaaacg tggcgtcatc gtccgtttcg
    10141 accatttccg aaaagaactc gggcataaac tctatgattt ctctggacgt ggtgttgtcg
    10201 aaactctcaa agtacgcagt caggaacgtg cgcgacatgt cgtcgggaaa ctcgcgcgga
    10261 aacatgttgt tgtaaccgaa cgggtcccat agcgccaaaa ccaaatctgc cagcgtcaat
    10321 agaatgagca cgatgccgac aatggagctg gcttggatag cgattcgagt taacggccgg
    10381 ccgtttaaac gaattcttcg aatgcatcta gatgcaccat tatgttccgg aggcgcgcca
    10441 tcgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
    10501 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
    10561 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
    10621 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
    10681 atctctacag gaacaggtgg tggcggccct cggtgcgctc gtactgctcc acgatggtgt
    10741 agtcctcgtt gtgggaggtg atgtccagct tggagtccac gtagtagtag ccgggcagct
    10801 gcacgggctt cttggccatg tagatggact tgaactccac caggtagtgg ccgccgtcct
    10861 tcagcttcag ggccttgtgg atctcgccct tcagcacgcc gtcgcggggg tacaggcgct
    10921 cggtggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg gaggggaagt
    10981 tcacgccgat gaacttcacc ttgtagatga agcagccgtc ctgcagggag gagtcctggg
    11041 tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
    11101 ggaaggacag cttcttgtag tcggggatgt cggcggggtg cttcacgtac accttggagc
    11161 cgtactggaa ctggggggac aggatgtccc aggcgaaggg cagggggccg cccttggtca
    11221 ccttcagctt cacggtgttg tggccctcgt aggggcggcc ctcgccctcg ccctcgatct
    11281 cgaactcgtg gccgttcacg gtgccctcca tgcgcacctt gaagcgcatg aactccttga
    11341 tgacgttctt ggaggagcgc accatggtgg cgaccggtgg atcccgggcc cgcggtaccg
    11401 tcgactctag cggtaccccg attgtttagc ttgttcagct gcgcttgttt atttgcttag
    11461 ctttcgctta gcgacgtgtt cactttgctt gtttgaattg aattgtcgct ccgtagacga
    11521 agcgcctcta tttatactcc ggcggtcgag ggttcctaaa tatagatcac ggatccgata
    11581 cgaatatgta tatgtatacc acttatatat atatatatca gaatgctttg catatatacg
    11641 gattttcggg gcgttggtat ttgttacgta acgccaattt tcatttagat atttgtgtac
    11701 atttctccag gtttctttgt gaaaaaatct acagactatt gatacgccaa aacaatgctt
    11761 attattacaa atgtagagag gttaaactgt caccacaact cactcaactg gtaactacta
    11821 ccgtttggaa cgcgatttcg atattttgaa caggtatctc gatatggtgg cgtgttctca
    11881 tacaagcaat tcacgagatc gcaatgattc ctttcagtaa acttcgaagg cggaacgatt
    11941 ttcgacaatc cacactaaat cgacaatcga aaattcgatt cgaattgacg cgaactttta
    12001 acgaatgcgt tatttttaaa attctcgaaa ttatttcgaa actaaagtca catacatatt
    12061 cgtctatatg taattcgtaa tttcgagaag ctttttggct catgctacca catggcacac
    12121 atagacataa tgtataagta ttggcgatct ctttctaatg tctttggtta tttttaaaac
    12181 atattcgaaa ttatttcgcc catgtatatg agccgacaac gaattcgagt ataatcaact
    12241 ttgtatacaa aagttgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
    12301 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg
    12361 gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt
    12421 ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
    12481 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
    12541 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
    12601 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
    12661 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
    12721 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
    12781 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
    12841 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtg
    12901 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
    12961 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
    13021 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
    13081 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggcacatat
    13141 acctgccgtt cactattatt tagtgaaatg agatattatg atattttctg aattgtgatt
    13201 aaaaaggcaa ctttatgccc atgcaacaga aactataaaa aatacagaga atgaaaagaa
    13261 acagatagat tttttagttc tttaggcccg tagtctgcaa atccttttat gattttctat
    13321 caaacaaaag aggaaaatag accagttgca atccaaacga gagtctaata gaatgaggtc
    13381 gaaaagtaaa tcgcgcgggt ttgttactga taaagcaggc aagacctaaa atgtgtaaag
    13441 ggcaaagtgt atactttggc gtcacccctt acatatttta ggtctttttt tattgtgcgt
    13501 aactaacttg ccatcttcaa acaggagggc tggaagaagc agaccgctaa cacagtacat
    13561 aaaaaaggag acatgaacga tgaacatcaa aaagtttgca aaacaagcaa cagtattaac
    13621 ctttactacc gcactgctgg caggaggcgc aactcaagcg tttgcgaaag aaacgaacca
    13681 aaagccatat aaggaaacat acggcatttc ccatattaca cgccatgata tgctgcaaat
    13741 ccctgaacag caaaaaaatg aaaaatatca agttcctgaa ttcgattcgt ccacaattaa
    13801 aaatatctct tctgcaaaag gcctggacgt ttgggacagc tggccattac aaaacgctga
    13861 cggcactgtc gcaaactatc acggctacca catcgtcttt gcattagccg gagatcctaa
    13921 aaatgcggat gacacatcga tttacatgtt ctatcaaaaa gtcggcgaaa cttctattga
    13981 cagctggaaa aacgctggcc gcgtctttaa agacagcgac aaattcgatg caaatgattc
    14041 tatcctaaaa gaccaaacac aagaatggtc aggttcagcc acatttacat ctgacggaaa
    14101 aatccgttta ttctacactg atttctccgg taaacattac ggcaaacaaa cactgacaac
    14161 tgcacaagtt aacgtatcag catcagacag ctctttgaac atcaacggtg tagaggatta
    14221 taaatcaatc tttgacggtg acggaaaaac gtatcaaaat gtacagcagt tcatcgatga
    14281 aggcaactac agctcaggcg acaaccatac gctgagagat cctcactacg tagaagataa
    14341 aggccacaaa tacttagtat ttgaagcaaa cactggaact gaagatggct accaaggcga
    14401 agaatcttta tttaacaaag catactatgg caaaagcaca tcattcttcc gtcaagaaag
    14461 tcaaaaactt ctgcaaagcg ataaaaaacg cacggctgag ttagcaaacg gcgctctcgg
    14521 tatgattgag ctaaacgatg attacacact gaaaaaagtg atgaaaccgc tgattgcatc
    14581 taacacagta acagatgaaa ttgaacgcgc gaacgtcttt aaaatgaacg gcaaatggta
    14641 cctgttcact gactcccgcg gatcaaaaat gacgattgac ggcattacgt ctaacgatat
    14701 ttacatgctt ggttatgttt ctaattcttt aactggccca tacaagccgc tgaacaaaac
    14761 tggccttgtg ttaaaaatgg atcttgatcc taacgatgta acctttactt actcacactt
    14821 cgctgtacct caagcgaaag gaaacaatgt cgtgattaca agctatatga caaacagagg
    14881 attctacgca gacaaacaat caacgtttgc gccaagcttc ctgctgaaca tcaaaggcaa
    14941 gaaaacatct gttgtcaaag acagcatcct tgaacaagga caattaacag ttaacaaata
    15001 aatgtcaggc tcccttatac acagccagtc tgcacgtcgc atagtgactg gatatgttgt
    15061 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
    15121 atatcatttt acgtttctcg ttcaactttt taatacaaag ttgcctccgg aacataatgg
    15181 tgcatctaga tgcccattcc tgtagacttc ttaattaaga cgtcagaatt ctcgaggcgg
    15241 ccgcatgtga gtctccctat agtgagtcgt attaatttcg cgggcgcgtc agcgggagag
    15301 ccgttgtaag gcggca
//
