LOCUS       sequence-405489-       14587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Viral silencing plasmid targeting the CrGLK gene from C. roseus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14587)
  AUTHORS   Cole-Osborn LF, McCallan SA, Prifti O, Abu R, Sjoelund V, 
            Lee-Parsons CWT
  TITLE     The role of the Golden2-like (GLK) transcription factor in 
            regulating terpenoid indole alkaloid biosynthesis in Catharanthus 
            roseus.
  JOURNAL   Plant Cell Rep. 2024 May 14;43(6):141. doi: 
            10.1007/s00299-024-03208-9.
  PUBMED    38743349
REFERENCE   2  (bases 1 to 14587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2024-05-14"; volume: "43(6):141. doi"; pages: " 
            10.1007/s00299-024-03208-9"
COMMENT     Sequence Label: pUN378 - pTRV2-CrGLK
FEATURES             Location/Qualifiers
     source          1..14587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    547..568
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(583..588,589..606,607..613)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    621..637
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     626..648
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     645..661
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     645..661
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        765..1110
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1053..1073
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    2773..2797
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2986..3010)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3541..3558)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3541..3557)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3550..3572)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3697..3716)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3907..3928
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    4175..4199
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(5795..6943)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(7242..8036)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  8228..8995
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      9618..10329
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(10456..10827)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            10860..10969
                     /note="incP origin of transfer"
     primer_bind     11192..11211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11445..11462
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(11650..12300)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    12765..12789
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        12913..13092
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        12945..13128
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             13149..13943
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13203..13222)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     13813..13832
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      14333..14585
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 gcctcctgtc aatgctggcg gcggctctgg tggtggttct ggtggcggct ctgagggtgg
       61 tggctctgag ggtggcggtt ctgagggtgg cggctctgag ggaggcggtt ccggtggtgg
      121 ctctggttcc ggtgattttg attatgaaaa gatggcaaac gctaataagg gggctatgac
      181 cgaaaatgcc gatgaaaacg cgctacagtc tgacgctaaa ggcaaacttg attctgtcgc
      241 tactgattac ggtgctgcta tcgatggttt cattggtgac gtttccggcc ttgctaatgg
      301 taatggtgct actggtgatt ttgctggctc taattcccaa atggctcaag tcggtgacgg
      361 tgataattca cctttaatga ataatttccg tcaatattta ccttccctcc ctcaatcggt
      421 tgaatgtcgc ccttttgtct ttggcccaat acgcaaaccg cctctccccg cgcgttggcc
      481 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
      541 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
      601 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
      661 ccatgattac gccaagcttg gctgcaggtc aacatggtgg agcacgacac tctcgtctac
      721 tccaagaata tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa
      781 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa
      841 aggacagtag aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct
      901 atcgttcaag atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac
      961 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     1021 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     1081 taaggaagtt catttcattt ggagaggata aaacattgca cctatggtgt tgccctggct
     1141 ggggtatgtc agtgatcgca gtagaatgta ctaattgaca agttggagaa tacggtagaa
     1201 cgtccttatc caacacagcc tttatccctc tccctgacga ggtttttgtc agtgtaatat
     1261 ttctttttga actatccagc ttagtaccgt acgggaaagt gactggtgtg cttatctttg
     1321 aaatgttact ttgggtttcg gttctttagg ttagtaagaa agcacttgtc ttctcataca
     1381 aaggaaaacc tgagacgtat cgcttacgaa agtagcaatg aaagaaaggt ggtggtttta
     1441 atcgctaccg caaaaacgat ggggtcgttt taattaactt ctcctacgca agcgtctaaa
     1501 cggacgttgg ggttttgcta gtttctttag agaaaactag ctaagtcttt aatgttatca
     1561 ttagagatgg cataaatata atacttgtgt ctgctgataa gatcatttta atttggacga
     1621 ttagacttgt tgaactacag gttactgaat cacttgcgct aatcaacatg ggagatatgt
     1681 acgatgaatc atttgacaag tcgggcggtc ctgctgactt gatggacgat tcttgggtgg
     1741 aatcagtttc gtggaaagat ctgttgaaga agttacacag cataaaattt gcactacagt
     1801 ctggtagaga tgagatcact gggttactag cggcactgaa tagacagtgt ccttattcac
     1861 catatgagca gtttccagat aagaaggtgt atttcctttt agactcacgg gctaacagtg
     1921 ctcttggtgt gattcagaac gcttcagcgt tcaagagacg agctgatgag aagaatgcag
     1981 tggcgggtgt tacaaatatt cctgcgaatc caaacacaac ggttacgacg aaccaaggga
     2041 gtactactac taccaaggcg aacactggct cgactttgga agaagacttg tacacttatt
     2101 acaaattcga tgatgcctct acagctttcc acaaatctct aacttcgtta gagaacatgg
     2161 agttgaagag ttattaccga aggaactttg agaaagtatt cgggattaag tttggtggag
     2221 cagctgctag ttcatctgca ccgcctccag cgagtggagg tccgatacgt cctaatccct
     2281 agggatttaa ggacgtgaac tctgttgaga tctctgtgaa attcagaggg tgggtgatac
     2341 catattcact gatgccatta gcgacatcta aatagggcta attgtgacta atttgaggga
     2401 atttccttta ccattgacgt cagtgtcgtt ggtagcattt gagtttcgca atgcacgaat
     2461 tacttaggaa gtggcttgac gacactaatg tgttattgtt agataatggt ttggtggtca
     2521 aggtacgtag tagagtccca catattcgca cgtatgaagt aattggaaag ttgtcagttt
     2581 ttgataattc actgggagat gatacgctgt ttgagggaaa agtagagaac gtatttgttt
     2641 ttatgttcag gcggttcttg tgtgtcaaca aagatggaca ttgttactca aggaagcacg
     2701 atgagcttta ttattacgga cgagtggact tagattctgt gagtaaggtt accgaattct
     2761 ctagaaggat caaccacttt gtacaagaaa gctgggtcct cagcttctct tgctagcaaa
     2821 tgtttcctgt gggatctgta tttttgaaga tggctagcaa tgttgtggcg agtaagacaa
     2881 tcaattccca taagttcaag aattcttgaa gggactgcct tatccacacc tagctgctcc
     2941 actgcttgta caaatcttct gtgcagctct ggtgtccaat ccaccagcct gcttttttgt
     3001 acaaacttgt tgatcctcca tggggatccg gtaccgagct cacgcgtctc gaggcccggg
     3061 catgtcccga agacattaaa ctacggttct ttaagtagat ccgtgtctga agttttaggt
     3121 tcaatttaaa cctacgagat tgacattctc gactgatctt gattgatcgg taagtctttt
     3181 gtaatttaat tttctttttg attttatttt aaattgttat ctgtttctgt gtatagactg
     3241 tttgagatcg gcgtttggcc gactcattgt cttaccatag gggaacggac tttgtttgtg
     3301 ttgttatttt atttgtattt tattaaaatt ctcaacgatc tgaaaaagcc tcgcggctaa
     3361 gagattgttg gggggtgagt aagtactttt aaagtgatga tggttacaaa ggcaaaaggg
     3421 gtaaaacccc tcgcctacgt aagcgttatt acgcccgtct gtacttatat cagtacactg
     3481 acgagtccct aaaggacgaa acgggagaac gctagccacc accaccacca cctcgaattc
     3541 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     3601 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     3661 cccttcccaa cagttgcgca gcctgaatgg cgcccgctcc tttcgctttc ttcccttcct
     3721 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3781 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     3841 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     3901 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt
     3961 ttgatttata agggattttg ccgatttcgg aaccaccatc aaacaggatt ttcgcctgct
     4021 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
     4081 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ccagtacatt aaaaacgtcc
     4141 gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac
     4201 cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca
     4261 gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca
     4321 tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat
     4381 ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaaat
     4441 atcatctccc tcgcagagat ccgaattatc agccttctta ttcatttctc gcttaaccgt
     4501 gacaggctgt cgatcttgag aactatgccg acataatagg aaatcgctgg ataaagccgc
     4561 tgaggaagct gagtggcgct atttctttag aagtgaacgt tgacgatatc aactccccta
     4621 tccattgctc accgaatggt acaggtcggg gacccgaagt tccgactgtc ggcctgatgc
     4681 atccccggct gatcgacccc agatctgggg ctgagaaagc ccagtaagga aacaactgta
     4741 ggttcgagtc gcgagatccc ccggaaccaa aggaagtagg ttaaacccgc tccgatcagg
     4801 ccgagccacg ccaggccgag aacattggtt cctgtaggca tcgggattgg cggatcaaac
     4861 actaaagcta ctggaacgag cagaagtcct ccggccgcca gttgccaggc ggtaaaggtg
     4921 agcagaggca cgggaggttg ccacttgcgg gtcagcacgg ttccgaacgc catggaaacc
     4981 gcccccgcca ggcccgctgc gacgccgaca ggatctagcg ctgcgtttgg tgtcaacacc
     5041 aacagcgcca cgcccgcagt tccgcaaata gcccccagga ccgccatcaa tcgtatcggg
     5101 ctacctagca gagcggcaga gatgaacacg accatcagcg gctgcacagc gcctaccgtc
     5161 gccgcgaccc cgcccggcag gcggtagacc gaaataaaca acaagctcca gaatagcgaa
     5221 atattaagtg cgccgaggat gaagatgcgc atccaccaga ttcccgttgg aatctgtcgg
     5281 acgatcatca cgagcaataa acccgccggc aacgcccgca gcagcatacc ggcgacccct
     5341 cggcctcgct gttcgggctc cacgaaaacg ccggacagat gcgccttgtg agcgtccttg
     5401 gggccgtcct cctgtttgaa gaccgacagc ccaatgatct cgccgtcgat gtaggcgccg
     5461 aatgccacgg catctcgcaa ccgttcagcg aacgcctcca tgggcttttt ctcctcgtgc
     5521 tcgtaaacgg acccgaacat ctctggagct ttcttcaggg ccgacaatcg gatctcgcgg
     5581 aaatcctgca cgtcggccgc tccaagccgt cgaatctgag ccttaatcac aattgtcaat
     5641 tttaatcctc tgtttatcgg cagttcgtag agcgcgccgt gcgtcccgag cgatactgag
     5701 cgaagcaagt gcgtcgagca gtgcccgctt gttcctgaaa tgccagtaaa gcgctggctg
     5761 ctgaaccccc agccggaact gaccccacaa ggccctagcg tttgcaatgc accaggtcat
     5821 cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga
     5881 cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc gcacatgagg cggaaggttt
     5941 ccagcttgag cgggtacggc tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg
     6001 tcggcgacag cttgcggtac ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca
     6061 gcacgacgat ttcctcgtcg atcaggacct ggcaacggga cgttttcttg ccacggtcca
     6121 ggacgcggaa gcggtgcagc agcgacaccg attccaggtg cccaacgcgg tcggacgtga
     6181 agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg taataccggc
     6241 cattgatcga ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc
     6301 cgataggggt gcgcttcgcg tactccaaca cctgctgcca caccagttcg tcatcgtcgg
     6361 cccgcagctc gacgccggtg taggtgatct tcacgtcctt gttgacgtgg aaaatgacct
     6421 tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg gcagagcggg
     6481 ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa
     6541 gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc ttggcctcgc
     6601 tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc atagttcctc
     6661 gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgaga cgacgcgaac
     6721 gctccacggc ggccgatggc gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct
     6781 cgatcttggc cgtagcttgc tggaccatcg agccgacgga ctggaaggtt tcgcggggcg
     6841 cacgcatgac ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga
     6901 tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt cattcaccct ccttgcggga
     6961 ttgccccgac tcacgccggg gcaatgtgcc cttattcctg atttgacccg cctggtgcct
     7021 tggtgtccag ataatccacc ttatcggcaa tgaagtcggt cccgtagacc gtctggccgt
     7081 ccttctcgta cttggtattc cgaatcttgc cctgcacgaa taccagcgac cccttgccca
     7141 aatacttgcc gtgggcctcg gcctgagagc caaaacactt gatgcggaag aagtcggtgc
     7201 gctcctgctt gtcgccggca tcgttgcgcc acatctaggt actaaaacaa ttcatccagt
     7261 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
     7321 tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca
     7381 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct
     7441 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc
     7501 ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc
     7561 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag
     7621 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg
     7681 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc
     7741 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca
     7801 aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc
     7861 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt
     7921 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg
     7981 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat
     8041 tatttccttc ctcttttcta cagtatttaa agatccccca agaagctaat tataacaaga
     8101 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt
     8161 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccac
     8221 aattatgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc
     8281 cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta
     8341 acggcaaaag caccgccgga catcagcgct atctctgctc tcactgccgt aaaacatggc
     8401 aactgcagtt cacttacacc gcttctcaac ccggtacgca ccagaaaatc attgatatgg
     8461 ccatgaatgg cgttggatgc cgggcaacag cccgcattat gggcgttggc ctcaacacga
     8521 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac agccgggcag
     8581 tgacgtcatc gtctgcgcgg aaatggacga acagtggggc tatgtcgggg ctaaatcgcg
     8641 ccagcgctgg ctgttttacg cgtatgacag tctccggaag acggttgttg cgcacgtatt
     8701 cggtgaacgc actatggcga cgctggggcg tcttatgagc ctgctgtcac cctttgacgt
     8761 ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg gaaagctgca
     8821 cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc tgaggcagca
     8881 cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa
     8941 agtcatcggg cattatctga acataaaaca ctatcaataa gttggagtca ttacccaatt
     9001 atgatagaat ttacaagcta taaggttatt gtcctgggtt tcaagcatta gtccatgcaa
     9061 gtttttatgc tttgcccatt ctatagatat attgataagc gcgctgccta tgccttgccc
     9121 cctgaaatcc ttacatacgg cgatatcttc tatataaaag atatattatc ttatcagtat
     9181 tgtcaatata ttcaaggcaa tctgcctcct catcctcttc atcctcttcg tcttggtagc
     9241 tttttaaata tggcgcttca tagagtaatt ctgtaaaggt ccaattctcg ttttcatacc
     9301 tcggtataat cttacctatc acctcaaatg gttcgctggg tttatcgcac ccccgaacac
     9361 gagcacggca cccgcgacca ctatgccaag aatgcccaag gtaaaaattg ccggccccgc
     9421 catgaagtcc gtgaatgccc cgacggccga agtgaagggc aggccgccac ccaggccgcc
     9481 gccctcactg cccggcacct ggtcgctgaa tgtcgatgcc agcacctgcg gcacgtcaat
     9541 gcttccgggc gtcgcgctcg ggctgatcgc ccatcccgtt actgccccga tcccggcaat
     9601 ggcaaggact gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg aggggcgcag
     9661 cccctggggg gatgggaggc ccgcgttagc gggccgggag ggttcgagaa gggggggcac
     9721 cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa acaaggttta
     9781 taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa aacgggcgga
     9841 aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc aataggtgcg
     9901 cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct catctgtcag
     9961 tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt gtccacatca
    10021 tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct ggccagctcc
    10081 acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg ggtgagtcgg
    10141 cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt tccgcgaggt
    10201 atccacaacg ccggcggccg cggtgtctcg cacacggctt cgacggcgtt tctggcgcgt
    10261 ttgcagggcc atagacggcc gccagcccag cggcgagggc aaccagcccg gtgagcgtcg
    10321 gaaaggcgct cggtcttgcc ttgctcgtcg gtgatgtact tcaccagctc cgcgaagtcg
    10381 ctcttcttga tggagcgcat ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt
    10441 tagcggctaa aaaagtcatg gctctgccct cgggcggacc acgcccatca tgaccttgcc
    10501 aagctcgtcc tgcttctctt cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa
    10561 ccgcgccgtg cgcgggtcgt cggtgagcca gagtttcagc aggccgccca ggcggcccag
    10621 gtcgccattg atgcgggcca gctcgcggac gtgctcatag tccacgacgc ccgtgatttt
    10681 gtagccctgg ccgacggcca gcaggtaggc cgacaggctc atgccggccg ccgccgcctt
    10741 ttcctcaatc gctcttcgtt cgtctggaag gcagtacacc ttgataggtg ggctgccctt
    10801 cctggttggc ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc
    10861 cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt
    10921 gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg
    10981 ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa
    11041 aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga
    11101 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    11161 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11221 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11281 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    11341 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    11401 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    11461 aggaagcgga agagcgccag aaggccgcca gagaggccga gcgcggccgt gaggcttgga
    11521 cgctagggca gggcatgaaa aagcccgtag cgggctgcta cgggcgtctg acgcggtgga
    11581 aagggggagg ggatgttgtc tacatggctc tgctgtagtg agtgggttgc gctccggcag
    11641 cggtcctgat caatcgtcac cctttctcgg tccttcaacg ttcctgacaa cgagcctcct
    11701 tttcgccaat ccatcgacaa tcaccgcgag tccctgctcg aacgctgcgt ccggaccggc
    11761 ttcgtcgaag gcgtctatcg cggcccgcaa cagcggcgag agcggagcct gttcaacggt
    11821 gccgccgcgc tcgccggcat cgctgtcgcc ggcctgctcc tcaagcacgg ccccaacagt
    11881 gaagtagctg attgtcatca gcgcattgac ggcgtccccg gccgaaaaac ccgcctcgca
    11941 gaggaagcga agctgcgcgt cggccgtttc catctgcggt gcgcccggtc gcgtgccggc
    12001 atggatgcgc gcgccatcgc ggtaggcgag cagcgcctgc ctgaagctgc gggcattccc
    12061 gatcagaaat gagcgccagt cgtcgtcggc tctcggcacc gaatgcgtat gattctccgc
    12121 cagcatggct tcggccagtg cgtcgagcag cgcccgcttg ttcctgaagt gccagtaaag
    12181 cgccggctgc tgaaccccca accgttccgc cagtttgcgt gtcgtcagac cgtctacgcc
    12241 gacctcgttc aacaggtcca gggcggcacg gatcactgta ttcggctgca actttgtcat
    12301 gcttgacact ttatcactga taaacataat atgtccacca acttatcagt gataaagaat
    12361 ccgcgcgttc aatcggacca gcggaggctg gtccggaggc cagacgtgaa acccaacata
    12421 cccctgatcg taattctgag cactgtcgcg ctcgacgctg tcggcatcgg cctgattatg
    12481 ccggtgctgc cgggcctcct gcgcgatctg gttcactcga acgacgtcac cgcccactat
    12541 ggcattctgc tggcgctgta tgcgttggtg caatttgcct gcgcacctgt gctgggcgcg
    12601 ctgtcggatc gtttcgggcg gcggccaatc ttgctcgtct cgctggccgg cgccagatct
    12661 ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc ttttcacgcc
    12721 cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat
    12781 atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg atcatgagcg
    12841 gagaattaag ggagtcacgt tatgaccccc gccgatgacg cgggacaagc cgttttacgt
    12901 ttggaactga cagaaccgca acgttgaagg agccactcag ccgcgggttt ctggagttta
    12961 atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac
    13021 tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata aattcccctc
    13081 ggtatccaat tagagtctca tattcactct caatccaaat aatctgcacc ggatctggat
    13141 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
    13201 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
    13261 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
    13321 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
    13381 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
    13441 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
    13501 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
    13561 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
    13621 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
    13681 tgcccgacgg cgatgatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
    13741 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
    13801 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
    13861 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
    13921 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
    13981 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
    14041 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
    14101 gttcttcgcc cacgggatct ctgcggaaca ggcggtcgaa ggtgccgata tcattacgac
    14161 agcaacggcc gacaagcaca acgccacgat cctgagcgac aatatgatcg ggcccggcgt
    14221 ccacatcaac ggcgtcggcg gcgactgccc aggcaagacc gagatgcacc gcgatatctt
    14281 gctgcgttcg gatattttcg tggagttccc gccacagacc cggatgatcc ccgatcgttc
    14341 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
    14401 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
    14461 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
    14521 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
    14581 agatcgg
//
