LOCUS       sequence-405490-       10496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For marking CRISPR HDR integrant with DsRed expressed in flight 
            muscles. Second U6 promoter for using two gRNAs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 10496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT30
FEATURES             Location/Qualifiers
     source          1..10496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             411..1202
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1448..2036
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1937..1956
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2190..2207
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2239..2257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2239..2256
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        2334..2754
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     protein_bind    2761..2884
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2907..2912,2913..2930,2931..2937)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        2980..3084
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3085..3153,3154..3945)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3303..3322)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     4466..4485
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4625..4748)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    4817..4951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4841..4860)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4895..4914
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(5059..5733,5734..5736,5737..5739))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(5121..5144)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(5319..5338)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5536..5556
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     regulatory      5736..5745
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    6610..6733
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6756..6761,6762..6779,6780..6786)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6840..7499
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6906..6925)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        7508..7953
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(7954..8040,8041..9375)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     protein_bind    complement(9414..9537)
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(9590..10386)
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     primer_bind     complement(10435..10454)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10436..10454)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
       61 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
      121 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
      181 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
      241 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
      301 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      361 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
      421 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
      481 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
      541 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
      601 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
      661 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
      721 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
      781 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
      841 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
      901 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
      961 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     1021 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1081 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1141 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     1201 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     1261 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     1321 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca
     1381 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1441 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1501 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1561 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1621 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1681 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1741 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1801 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1861 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1921 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1981 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2041 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2101 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2161 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa atcgtcacct
     2221 gcaggaaggt ttaaacgcat ttaggtgaca ctatagaagt gtgtatcgct cgagggatcc
     2281 gaattcgaag tcttggtacg gaggggatcc gatcgacaag ccgaacatat gggttttttg
     2341 ctcacctgtg attgctccta ctcaaataca aaaacatcaa attttctgtc aataaagcat
     2401 atttatttat atttatttta caggaaagaa ttccttttaa agtgtatttt aacctataat
     2461 gaaaaacgat taaaaaaaat acataaaata attcgaaaat ttttgaatag cccaggttga
     2521 taaaaattca tttcatacgt tttataactt atgcccctaa gtattttttg accatagtgt
     2581 ttcaattcta cattaatttt acagagtaga atgaaacgcc acctactcag ccaagaggcg
     2641 aaaaggttag ctcgccaagc agagagggcg ccagtgctca ctacttttta taattctcaa
     2701 cttctttttc cagactcagt tcgtatatat agacctattt tcaatttaac gtcggggcct
     2761 caactttgta taataaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     2821 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     2881 tatgggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
     2941 ggattttgag ttaggatccg tcgagatttt caggagctac gcggaacccc tatttgttta
     3001 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3061 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3121 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3181 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3241 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3301 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3361 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3421 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3481 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3541 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3601 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3661 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3721 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3781 tctggagccg gtgagcgtgg gtcccgcggt atcattgcag cactggggcc agatggtaag
     3841 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3901 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaatggat ccggcttact
     3961 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     4021 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     4081 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     4141 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     4201 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     4261 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     4321 tatcgtctgt ttgtggatgt acagagtgat attatcgata caccgggccg tcgcatggtt
     4381 attccacttg cgtctgcgcg cttgctctca gataaagtct cccgtgaact ttacccggtg
     4441 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtt
     4501 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     4561 attaacctga tgttctgggg aatataatgt caggctccct tatacacagc cagtctgcac
     4621 gtcgcatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     4681 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa cttttctata
     4741 caaagttgtc cagctccgag ctcggtacct cgcgaatgca tctagatgca ccattatgtt
     4801 ccggaggcgc gccatcgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     4861 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4921 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     4981 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     5041 tatggctgat tatgatctct acaggaacag gtggtggcgg ccctcggtgc gctcgtactg
     5101 ctccacgatg gtgtagtcct cgttgtggga ggtgatgtcc agcttggagt ccacgtagta
     5161 gtagccgggc agctgcacgg gcttcttggc catgtagatg gacttgaact ccaccaggta
     5221 gtggccgccg tccttcagct tcagggcctt gtggatctcg cccttcagca cgccgtcgcg
     5281 ggggtacagg cgctcggtgg aggcctccca gcccatggtc ttcttctgca ttacggggcc
     5341 gtcggagggg aagttcacgc cgatgaactt caccttgtag atgaagcagc cgtcctgcag
     5401 ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca
     5461 cttgaagccc tcggggaagg acagcttctt gtagtcgggg atgtcggcgg ggtgcttcac
     5521 gtacaccttg gagccgtact ggaactgggg ggacaggatg tcccaggcga agggcagggg
     5581 gccgcccttg gtcaccttca gcttcacggt gttgtggccc tcgtaggggc ggccctcgcc
     5641 ctcgccctcg atctcgaact cgtggccgtt cacggtgccc tccatgcgca ccttgaagcg
     5701 catgaactcc ttgatgacgt tcttggagga gcgcaccatg gtggcgaccg gtggatcccg
     5761 ggcccgcggt accgtcgact ctagcggtac cccgattgtt tagcttgttc agctgcgctt
     5821 gtttatttgc ttagctttcg cttagcgacg tgttcacttt gcttgtttga attgaattgt
     5881 cgctccgtag acgaagcgcc tctatttata ctccggcggt cgagggttcc taaatataga
     5941 tcacggatcc gatacgaata tgtatatgta taccacttat atatatatat atcagaatgc
     6001 tttgcatata tacggatttt cggggcgttg gtatttgtta cgtaacgcca attttcattt
     6061 agatatttgt gtacatttct ccaggtttct ttgtgaaaaa atctacagac tattgatacg
     6121 ccaaaacaat gcttattatt acaaatgtag agaggttaaa ctgtcaccac aactcactca
     6181 actggtaact actaccgttt ggaacgcgat ttcgatattt tgaacaggta tctcgatatg
     6241 gtggcgtgtt ctcatacaag caattcacga gatcgcaatg attcctttca gtaaacttcg
     6301 aaggcggaac gattttcgac aatccacact aaatcgacaa tcgaaaattc gattcgaatt
     6361 gacgcgaact tttaacgaat gcgttatttt taaaattctc gaaattattt cgaaactaaa
     6421 gtcacataca tattcgtcta tatgtaattc gtaatttcga gaagcttttt ggctcatgct
     6481 accacatggc acacatagac ataatgtata agtattggcg atctctttct aatgtctttg
     6541 gttattttta aaacatattc gaaattattt cgcccatgta tatgagccga caacgaattc
     6601 gagtataatc aactttgtat acaaaagttg aacgagaaac gtaaaatgat ataaatatca
     6661 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
     6721 tccagtcact atgggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     6781 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
     6841 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     6901 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     6961 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     7021 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     7081 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     7141 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     7201 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     7261 atatgttttt cgtgtcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     7321 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     7381 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     7441 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     7501 cgcgtggcac atatacctgc cgttcactat tatttagtga aatgagatat tatgatattt
     7561 tctgaattgt gattaaaaag gcaactttat gcccatgcaa cagaaactat aaaaaataca
     7621 gagaatgaaa agaaacagat agatttttta gttctttagg cccgtagtct gcaaatcctt
     7681 ttatgatttt ctatcaaaca aaagaggaaa atagaccagt tgcaatccaa acgagagtct
     7741 aatagaatga ggtcgaaaag taaatcgcgc gggtttgtta ctgataaagc aggcaagacc
     7801 taaaatgtgt aaagggcaaa gtgtatactt tggcgtcacc ccttacatat tttaggtctt
     7861 tttttattgt gcgtaactaa cttgccatct tcaaacagga gggctggaag aagcagaccg
     7921 ctaacacagt acataaaaaa ggagacatga acgatgaaca tcaaaaagtt tgcaaaacaa
     7981 gcaacagtat taacctttac taccgcactg ctggcaggag gcgcaactca agcgtttgcg
     8041 aaagaaacga accaaaagcc atataaggaa acatacggca tttcccatat tacacgccat
     8101 gatatgctgc aaatccctga acagcaaaaa aatgaaaaat atcaagttcc tgaattcgat
     8161 tcgtccacaa ttaaaaatat ctcttctgca aaaggcctgg acgtttggga cagctggcca
     8221 ttacaaaacg ctgacggcac tgtcgcaaac tatcacggct accacatcgt ctttgcatta
     8281 gccggagatc ctaaaaatgc ggatgacaca tcgatttaca tgttctatca aaaagtcggc
     8341 gaaacttcta ttgacagctg gaaaaacgct ggccgcgtct ttaaagacag cgacaaattc
     8401 gatgcaaatg attctatcct aaaagaccaa acacaagaat ggtcaggttc agccacattt
     8461 acatctgacg gaaaaatccg tttattctac actgatttct ccggtaaaca ttacggcaaa
     8521 caaacactga caactgcaca agttaacgta tcagcatcag acagctcttt gaacatcaac
     8581 ggtgtagagg attataaatc aatctttgac ggtgacggaa aaacgtatca aaatgtacag
     8641 cagttcatcg atgaaggcaa ctacagctca ggcgacaacc atacgctgag agatcctcac
     8701 tacgtagaag ataaaggcca caaatactta gtatttgaag caaacactgg aactgaagat
     8761 ggctaccaag gcgaagaatc tttatttaac aaagcatact atggcaaaag cacatcattc
     8821 ttccgtcaag aaagtcaaaa acttctgcaa agcgataaaa aacgcacggc tgagttagca
     8881 aacggcgctc tcggtatgat tgagctaaac gatgattaca cactgaaaaa agtgatgaaa
     8941 ccgctgattg catctaacac agtaacagat gaaattgaac gcgcgaacgt ctttaaaatg
     9001 aacggcaaat ggtacctgtt cactgactcc cgcggatcaa aaatgacgat tgacggcatt
     9061 acgtctaacg atatttacat gcttggttat gtttctaatt ctttaactgg cccatacaag
     9121 ccgctgaaca aaactggcct tgtgttaaaa atggatcttg atcctaacga tgtaaccttt
     9181 acttactcac acttcgctgt acctcaagcg aaaggaaaca atgtcgtgat tacaagctat
     9241 atgacaaaca gaggattcta cgcagacaaa caatcaacgt ttgcgccaag cttcctgctg
     9301 aacatcaaag gcaagaaaac atctgttgtc aaagacagca tccttgaaca aggacaatta
     9361 acagttaaca aataaatgtc aggctccctt atacacagcc agtctgcacg tcgcatagtg
     9421 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
     9481 atatattgat atttatatca ttttacgttt ctcgttcaac tttttaatac aaagttgcct
     9541 ccggaacata atggtgcatc tagatgccca ttcctgtaga cttcttaatc gaagttcacc
     9601 cggatatctt tcctatatat actgtactct tgcagaatat gtacttggga atcataagga
     9661 tgcgaaggac gtacgcgctg tgtctgtaac taaggggttg ctttcgggta cctaccatgc
     9721 caatgatggg actaccgccg gataggaatc ccttttgggt gcacaccgtc acctccagtt
     9781 ccgtctgcct gagttcggca atggaaatca ggtactcaaa ggatgcatcg tagacggggt
     9841 tgcagttgtc cttgatcacg ctcgtcttgc gtttcgactc cttggtgcgt ccaggcaaca
     9901 gatacagctt aacatacgga tcggggatat tgctgggatc gcgaagtggt atcttctgga
     9961 ttttgtgtat ggtcacgtct agtttttgac gctgggcgct gtagcggatc gacaactgca
    10021 ttcgtccaag gccatgttcg ccgggtgagg aggtggagtc tggaaagcgg tgggtcagca
    10081 cagaggtggc cagctcgctg atgggttttt cgctggaggt ggaggccgac atggccgggg
    10141 agattttcgt agccgccaca ggatcttctt cactgatggg cgattcggcg gacaaacgct
    10201 tgctggatgc ctgagattcc taaaagcaat ggcaaattag attcaattaa aacagaaatt
    10261 gtttatataa cagagtaaaa gcaaaaaaaa aggaatcaca gattctcccc gacggggaat
    10321 tgaaccccgt tctcccgcgt gacaggcggg gatactaacc actatactat cgaggacgtt
    10381 gaaaatgaat tcaattaaga cgtcagaatt ctcgaggcgg ccgcatgtga gtctccctat
    10441 agtgagtcgt attaatttcg cgggcgcgtc agcgggagag ccgttgtaag gcggca
//
