LOCUS       sequence-405492-        2301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 3b part for Modular Cloning in Yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Simakin P, Koch C, Herrmann JM
  TITLE     A modular cloning (MoClo) toolkit for reliable intracellular protein
            targeting in the yeast Saccharomyces cerevisiae.
  JOURNAL   Microb Cell. 2023 Feb 28;10(4):78-87. doi: 10.15698/mic2023.04.794. 
            eCollection 2023 Apr 3.
  PUBMED    37009624
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15698/mic2023.04.794"; journalName: "Microb Cell"; date: 
            "2023-02-28- 28"; volume: "10"; issue: "4"; pages: "78-87"
COMMENT     Sequence Label: pHHYTK122
FEATURES             Location/Qualifiers
     source          1..2301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(666..700)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(771..1430)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1345..1364
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1612..2200
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2101..2120
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat tcttctaaag gtgaagaatt gtttactggt gttgttccaa ttttggttga
       61 attggatggt gatgttaatg gtcataaatt ttctgttaga ggtgaaggtg aaggtgatgc
      121 tactattggt aaattgactt tgaaatttat ttgtactact ggtaaattgc cagttccatg
      181 gccaactttg gttactactt tgacttatgg tgttcaatgt ttttctagat atccagatca
      241 tatgaaacaa catgattttt ttaaatctgc tatgccagaa ggttatgttc aagaaagaac
      301 tayttctttt aaagatgatg gtaaatataa aactagagct gttgttaaat ttgaaggtga
      361 tactttggtt aatagaattg aattgaaagg tactgatttt aaagaagatg gtaatatttt
      421 gggtcataaa ttggaatata attttaattc tcataatgtt tatattactg ctgataaaca
      481 aaaaaatggt attaaagcta attttactgt tagacataat gttgaagatg gttctgttca
      541 attggctgat cattatcaac aaaatactcc aattggtgat ggtccagttt tgttgccaga
      601 taatcattat ttgtctactc aaactgtttt gtctaaagat ccaaatggat cctgagacca
      661 gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
      721 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
      781 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
      841 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
      901 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
      961 tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac
     1021 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     1081 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     1141 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     1201 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     1261 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     1321 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     1381 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     1441 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     1501 tggtgaaagt tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga
     1561 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1621 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1681 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1741 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1801 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1861 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1921 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1981 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2041 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2101 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2161 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2221 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2281 tgattctgtg gataaccgta g
//
