LOCUS       sequence-405498-       11903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for PE protein in bacteria..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11903)
  AUTHORS   Ponnienselvan K, Liu P, Nyalile T, Oikemus S, Maitland SA, Lawson 
            ND, Luban J, Wolfe SA
  TITLE     Reducing the inherent auto-inhibitory interaction within the pegRNA 
            enhances prime editing efficiency.
  JOURNAL   Nucleic Acids Res. 2023 May 29:gkad456. doi: 10.1093/nar/gkad456.
  PUBMED    37246708
REFERENCE   2  (bases 1 to 11903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 May 29:gkad456. doi: 10.1093/nar/gkad456."
COMMENT     Sequence Label: PE-Max-pet21A
FEATURES             Location/Qualifiers
     source          1..11903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     939..958
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(978..1055)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1261..1280
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1364..1383
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1364..1382
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1383..1407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1422..1444
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1452..1484
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             1494..1520
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             1566..1586
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1587..5687
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5730..5756
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5778..5798
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7911..7931
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7944..7991
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7998..8015
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8068..8086)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8082..8129
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      8166..8621
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8253..8272)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8463..8484
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8647..8751
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8752..8820,8821..9612)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8970..8989)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9783..10371
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10272..10291
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10525..10542
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10557..10696
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10622..10641)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10782..10804
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(10798..10989)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(11798..11903,1..977))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt
       61 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt
      121 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc
      181 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg
      241 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga
      301 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga
      361 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc
      421 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta
      481 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat
      541 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga
      601 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag
      661 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg
      721 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca
      781 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa
      841 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc
      901 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat
      961 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc
     1021 ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg
     1081 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
     1141 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
     1201 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
     1261 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
     1321 gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata
     1381 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
     1441 gagatataca tatggctagc atgactggtg gacagcaaat gggtcgcgcc gctccggcag
     1501 ctaagaaaaa gaaactggat ggcagcgtcg acaaacggac agccgacgga agcgagttcg
     1561 agtcaccaaa gaagaagcgg aaagtcgaca agaagtacag catcggcctg gacatcggca
     1621 ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca
     1681 aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt
     1741 tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca
     1801 ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg
     1861 tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag gataagaagc
     1921 acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac gagaagtacc
     1981 ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc gacctgcggc
     2041 tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg
     2101 acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg cagacctaca
     2161 accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag gccatcctgt
     2221 ctgccagact gagcaagagc agaaagctgg aaaatctgat cgcccagctg cccggcgaga
     2281 agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc cccaacttca
     2341 agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac acctacgacg
     2401 acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg tttctggccg
     2461 ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac accgagatca
     2521 ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac caggacctga
     2581 ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag attttcttcg
     2641 accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag gaagagttct
     2701 acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc
     2761 tgaagagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc atcccccacc
     2821 agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt tacccattcc
     2881 tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc tactacgtgg
     2941 gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc gaggaaacca
     3001 tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag agcttcatcg
     3061 agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc
     3121 tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac gtgaccgagg
     3181 gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt
     3241 tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg
     3301 agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca
     3361 cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg
     3421 aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg
     3481 aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc
     3541 ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca
     3601 agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact
     3661 tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg
     3721 tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca
     3781 ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc
     3841 ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg
     3901 gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca
     3961 gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc
     4021 tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc
     4081 tgtccgacta cgatgtggac gctatcgtgc ctcagagctt tctgaaggac gactccatcg
     4141 acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg
     4201 aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta
     4261 cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata
     4321 aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac
     4381 agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag
     4441 tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt
     4501 acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg
     4561 tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact
     4621 acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta
     4681 ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg
     4741 ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg
     4801 tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga
     4861 atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca
     4921 agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg
     4981 gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca
     5041 agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca
     5101 gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg
     5161 acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa
     5221 tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc tccaaatatg
     5281 tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg
     5341 agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga
     5401 tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg
     5461 cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt
     5521 ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc accatcgacc
     5581 ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca
     5641 ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggtgactcc ggcggaagct
     5701 ctggtggcag caagcggacc gccggctctt acccatacga tgttccagat tacgctgacg
     5761 gctctgaatt cgagagccct aagaagaaaa gaaaggtgag cggaggctct agcggcggaa
     5821 gcaccctgaa cattgaagac gagtatagac tgcatgaaac aagcaaggaa cccgacgtgt
     5881 ccctgggctc cacctggctg tccgactttc cccaggcctg ggccgagaca ggaggaatgg
     5941 gcctggccgt gcggcaggca cccctgatca tccctctgaa ggccacctct acacccgtga
     6001 gcatcaagca gtaccctatg tctcaggagg ccagactggg catcaagcct cacatccaga
     6061 ggctgctgga ccagggcatc ctggtgccat gccagagccc ctggaacaca ccactgctgc
     6121 ccgtgaagaa gccaggcacc aatgactata gacccgtgca ggatctgaga gaggtgaaca
     6181 agagggtgga ggatatccac cccaccgtgc ccaaccctta caatctgctg tccggcctgc
     6241 ccccttctca ccagtggtat acagtgctgg acctgaagga tgccttcttt tgtctgagac
     6301 tgcaccctac cagccagcca ctgttcgcct ttgagtggag ggaccctgag atgggcatct
     6361 ctggccagct gacctggaca cgcctgcctc agggcttcaa gaatagccca acactgttta
     6421 acgaggccct gcaccgcgac ctggcagatt tccggatcca gcacccagat ctgatcctgc
     6481 tgcagtacgt ggacgatctg ctgctggccg ccaccagcga gctggattgc cagcagggaa
     6541 cacgcgccct gctgcagacc ctgggaaacc tgggatatag ggcatccgcc aagaaggccc
     6601 agatctgtca gaagcaggtg aagtacctgg gctatctgct gaaggagggc cagagatggc
     6661 tgacagaggc caggaaggag acagtgatgg gccagccaac acccaagacc ccaagacagc
     6721 tgagggagtt cctgggcaaa gcaggatttt gcaggctgtt catcccagga ttcgcagaga
     6781 tggcagcacc tctgtaccca ctgaccaagc cgggcaccct gtttaattgg ggccctgacc
     6841 agcagaaggc ctatcaggag atcaagcagg ccctgctgac agcaccagcc ctgggcctgc
     6901 cagacctgac caagcctttc gagctgtttg tggatgagaa gcagggctac gccaagggcg
     6961 tgctgaccca gaagctggga ccatggagac ggcccgtggc ctatctgtcc aagaagctgg
     7021 acccagtggc agcaggatgg ccaccatgcc tgaggatggt ggcagcaatc gccgtgctga
     7081 caaaggatgc cggcaagctg accatgggac agccactggt catcctggca ccacacgcag
     7141 tggaggccct ggtgaagcag cctccagatc gctggctgtc taacgcccgg atgacacact
     7201 accaggccct gctgctggac accgatcgcg tgcagtttgg ccctgtggtg gccctgaatc
     7261 cagccaccct gctgcctctg ccagaggagg gcctgcagca caactgtctg gacatcctgg
     7321 cagaggcaca cggaacaagg ccagacctga ccgatcagcc cctgcctgac gccgatcaca
     7381 catggtatac cgatggaagc tccctgctgc aggagggcca gaggaaggca ggagcagcag
     7441 tgaccacaga gacagaagtg atctgggcca aggccctgcc agcaggcaca tccgcccagc
     7501 gggccgagct gatcgccctg acccaggccc tgaagatggc cgagggcaag aagctgaacg
     7561 tgtacacaga ctccagatat gccttcgcca ccgcacacat ccacggagag atctacaggc
     7621 gccggggctg gctgacctct gagggcaagg agatcaagaa caaggatgag atcctggccc
     7681 tgctgaaggc cctgtttctg cccaagcggc tgagcatcat ccactgtcct ggacaccaga
     7741 agggacactc cgccgaggca aggggcaatc ggatggccga ccaggccgcc agaaaggctg
     7801 ctattactga aactcccgac acttccactc tgctgattga aaactcctcc ccttctggcg
     7861 gctcaaccgg tggtggtccc gggggtggtg cggccgcagg cagcggaagc cctaagaaaa
     7921 aacgaaaagt tggcagcgga agcaaaaggc cggcggccac gaaaaaggcc ggccaggcaa
     7981 aaaagaaaaa gctcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
     8041 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     8101 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga
     8161 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     8221 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     8281 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     8341 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     8401 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     8461 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     8521 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     8581 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     8641 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8701 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8761 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8821 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8881 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8941 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     9001 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     9061 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     9121 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     9181 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     9241 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     9301 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     9361 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     9421 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     9481 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     9541 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     9601 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     9661 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9721 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9781 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9841 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9901 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     9961 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10021 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10081 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10141 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10201 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    10261 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    10321 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    10381 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    10441 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    10501 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
    10561 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca
    10621 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
    10681 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
    10741 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
    10801 gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg
    10861 gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc
    10921 cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg
    10981 tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat
    11041 gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga
    11101 acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca
    11161 gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca
    11221 tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact
    11281 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
    11341 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
    11401 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc
    11461 cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac
    11521 gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt
    11581 cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc
    11641 tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc
    11701 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc
    11761 ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    11821 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    11881 tattgggcgc cagggtggtt ttt
//
