LOCUS       sequence-405507-       10135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For inserting a phiC31 attP site. HDR integrant is marked with 
            removable ie1-DsRed cassette, which is expressed in abdomen and 
            mouthparts..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10135)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 10135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT11
FEATURES             Location/Qualifiers
     source          1..10135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             411..1202
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1448..2036
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1937..1956
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2190..2207
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2239..2257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2239..2256
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        2334..2754
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     protein_bind    2761..2884
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2907..2912,2913..2930,2931..2937)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        2980..3084
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3085..3153,3154..3945)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3303..3322)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     4466..4485
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4625..4748)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    4821..4920
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     promoter        5249..5840
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     primer_bind     complement(6212..6232)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     polyA_signal    6817..6951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6854..6873)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6908..6927
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    7055..7178
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7201..7206,7207..7224,7225..7231)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7285..7944
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7351..7370)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        7953..8398
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(8399..8485,8486..9820)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     protein_bind    complement(9859..9982)
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(10074..10093)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10075..10093)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
       61 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
      121 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
      181 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
      241 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
      301 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      361 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
      421 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
      481 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
      541 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
      601 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
      661 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
      721 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
      781 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
      841 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
      901 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
      961 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     1021 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1081 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1141 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     1201 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     1261 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     1321 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca
     1381 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1441 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1501 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1561 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1621 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1681 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1741 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1801 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1861 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1921 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1981 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2041 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2101 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2161 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa atcgtcacct
     2221 gcaggaaggt ttaaacgcat ttaggtgaca ctatagaagt gtgtatcgct cgagggatcc
     2281 gaattcgaag tcttggtacg gaggggatcc gatcgacaag ccgaacatat gggttttttg
     2341 ctcacctgtg attgctccta ctcaaataca aaaacatcaa attttctgtc aataaagcat
     2401 atttatttat atttatttta caggaaagaa ttccttttaa agtgtatttt aacctataat
     2461 gaaaaacgat taaaaaaaat acataaaata attcgaaaat ttttgaatag cccaggttga
     2521 taaaaattca tttcatacgt tttataactt atgcccctaa gtattttttg accatagtgt
     2581 ttcaattcta cattaatttt acagagtaga atgaaacgcc acctactcag ccaagaggcg
     2641 aaaaggttag ctcgccaagc agagagggcg ccagtgctca ctacttttta taattctcaa
     2701 cttctttttc cagactcagt tcgtatatat agacctattt tcaatttaac gtcggggcct
     2761 caactttgta taataaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     2821 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     2881 tatgggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
     2941 ggattttgag ttaggatccg tcgagatttt caggagctac gcggaacccc tatttgttta
     3001 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3061 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3121 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3181 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3241 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3301 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3361 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3421 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3481 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3541 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3601 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3661 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3721 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3781 tctggagccg gtgagcgtgg gtcccgcggt atcattgcag cactggggcc agatggtaag
     3841 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3901 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaatggat ccggcttact
     3961 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     4021 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     4081 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     4141 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     4201 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     4261 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     4321 tatcgtctgt ttgtggatgt acagagtgat attatcgata caccgggccg tcgcatggtt
     4381 attccacttg cgtctgcgcg cttgctctca gataaagtct cccgtgaact ttacccggtg
     4441 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtt
     4501 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     4561 attaacctga tgttctgggg aatataatgt caggctccct tatacacagc cagtctgcac
     4621 gtcgcatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     4681 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa cttttctata
     4741 caaagttgtc cagctgtact gacggacaca ccgaagcccc ggcggcaacc ctcagcggat
     4801 gccccggggc ttcacgtttt cccaggtcag aagcggtttt cgggagtagt gccccaactg
     4861 gggtaacctt tgagttctct cagttggggg cgtagggtcg ccgacatgac acaaggggtt
     4921 gtgaccgggg tggacacgta cgcgggtgct tacgaccgtc agtcgcgcga gcgcgaggtt
     4981 gcttaagaat aagtaattct taagcaacca ttacgccaag cttgcatgcc gagctcttac
     5041 gcgcccgcgt aaaacacaat caagtatgag tcataagctg atgtcatgtt ttgcacacgg
     5101 ctcataaccg aactggcttt acgagtagaa ttctacttgt aacgcacgat cagtggatga
     5161 tgtcatttgt ttttcaaatc gagatgatgt catgttttgc acatggctca taactaaact
     5221 cgctttacgg gtagaattct acgcgcgtcg atgtctttgt gatgcgcgcg acatttttgt
     5281 aggttattga taaaatgaac ggatacgttg cccgacatta tcattaaatc cttggcgtag
     5341 aatttgtcgg gtccattgtc cgtgtgcgct agcatgcccg taacggacct cgtacttttg
     5401 gcttcaaagg ttttgcgcac agacaaaatg tgccacactt gcagctctgc atgtgtgcgc
     5461 gttaccacaa atcccaacgg cgcagtgtac ttgttgtatg caaataaatc tcgataaagg
     5521 cgcggcgcgc gaatgcagct gatcacgtac gctcctcgtg ttccgttcaa ggacggtgtt
     5581 atcgacctca gattaatgtt tatcggccga ctgttttcgt atccgctcac caaacgcgtt
     5641 tttgcattaa cattgtatgt cggcggatgt tctatatcta atttgaataa ataaacgata
     5701 accgcgttgg ttttagaggg cataataaaa gaaatattgt tatcgtgttc gccattaggg
     5761 cagtataaat tgacgttcat gttggatatt gtttcagttg caagttgaca ctggcggcga
     5821 caagatcgtg aacaaccaag tgaccattaa ccctcgaccg ccggagtata aatagaggcg
     5881 cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa
     5941 agctaagcaa ataaacaagc gcagctgaac aagctaaaca atcggggtac cgctagagtc
     6001 gacggtacga tccaccggtc gccaccccat ggtgcgctcc tccaagaacg tcatcaagga
     6061 gttcatgcgc ttcaaggtgc gcatggaggg caccgtgaac ggccacgagt tcgagatcga
     6121 gggcgagggc gagggccgcc cctacgaggg ccacaacacc gtgaagctga aggtgaccaa
     6181 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttccagt acggctccaa
     6241 ggtgtacgtg aagcaccccg ccgacatccc cgactacaag aagctgtcct tccccgaggg
     6301 cttcaagtgg gagcgcgtga tgaatttcga agatggtgga gttgtcacag tcacacaaga
     6361 ttcttcgctc caagatggtt gttttattta caaagtgaaa tttattggag tcaattttcc
     6421 gtcggatgga ccggttatgc aaaagaaaac aatgggttgg gaagcatcga cggaacgtct
     6481 gtatccacga gatggagttt tgaaaggcga aattcacaaa gctcttaaac tcaaagatgg
     6541 cggacattac cttgtagaat ttaaaagcat ttatatggct aagaaaccgg tccaactccc
     6601 tggatattat tatgtcgatt cgaaactgga tattacttca cataacgaag attatacgat
     6661 agttgagcaa tatgaacgca ctgaaggtcg tcaccatctc tttctctaaa gatcataatc
     6721 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     6781 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     6841 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6901 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat cgatggcgcg
     6961 cctccggaac ataatggtgc atctagatgc attcgcgagg taccgagctc gaattcactg
     7021 gggttgctta agaataagta attcttaagc aacccaactt tgtatacaaa agttgaacga
     7081 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
     7141 acataatact gtaaaacaca acatatccag tcactatggg ccgcattagg caccccaggc
     7201 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga
     7261 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     7321 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     7381 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     7441 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     7501 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     7561 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     7621 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     7681 tatttcccta aagggtttat tgagaatatg tttttcgtgt cagccaatcc ctgggtgagt
     7741 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     7801 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     7861 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     7921 gatgagtggc agggcggggc gtaaacgcgt ggcacatata cctgccgttc actattattt
     7981 agtgaaatga gatattatga tattttctga attgtgatta aaaaggcaac tttatgccca
     8041 tgcaacagaa actataaaaa atacagagaa tgaaaagaaa cagatagatt ttttagttct
     8101 ttaggcccgt agtctgcaaa tccttttatg attttctatc aaacaaaaga ggaaaataga
     8161 ccagttgcaa tccaaacgag agtctaatag aatgaggtcg aaaagtaaat cgcgcgggtt
     8221 tgttactgat aaagcaggca agacctaaaa tgtgtaaagg gcaaagtgta tactttggcg
     8281 tcacccctta catattttag gtcttttttt attgtgcgta actaacttgc catcttcaaa
     8341 caggagggct ggaagaagca gaccgctaac acagtacata aaaaaggaga catgaacgat
     8401 gaacatcaaa aagtttgcaa aacaagcaac agtattaacc tttactaccg cactgctggc
     8461 aggaggcgca actcaagcgt ttgcgaaaga aacgaaccaa aagccatata aggaaacata
     8521 cggcatttcc catattacac gccatgatat gctgcaaatc cctgaacagc aaaaaaatga
     8581 aaaatatcaa gttcctgaat tcgattcgtc cacaattaaa aatatctctt ctgcaaaagg
     8641 cctggacgtt tgggacagct ggccattaca aaacgctgac ggcactgtcg caaactatca
     8701 cggctaccac atcgtctttg cattagccgg agatcctaaa aatgcggatg acacatcgat
     8761 ttacatgttc tatcaaaaag tcggcgaaac ttctattgac agctggaaaa acgctggccg
     8821 cgtctttaaa gacagcgaca aattcgatgc aaatgattct atcctaaaag accaaacaca
     8881 agaatggtca ggttcagcca catttacatc tgacggaaaa atccgtttat tctacactga
     8941 tttctccggt aaacattacg gcaaacaaac actgacaact gcacaagtta acgtatcagc
     9001 atcagacagc tctttgaaca tcaacggtgt agaggattat aaatcaatct ttgacggtga
     9061 cggaaaaacg tatcaaaatg tacagcagtt catcgatgaa ggcaactaca gctcaggcga
     9121 caaccatacg ctgagagatc ctcactacgt agaagataaa ggccacaaat acttagtatt
     9181 tgaagcaaac actggaactg aagatggcta ccaaggcgaa gaatctttat ttaacaaagc
     9241 atactatggc aaaagcacat cattcttccg tcaagaaagt caaaaacttc tgcaaagcga
     9301 taaaaaacgc acggctgagt tagcaaacgg cgctctcggt atgattgagc taaacgatga
     9361 ttacacactg aaaaaagtga tgaaaccgct gattgcatct aacacagtaa cagatgaaat
     9421 tgaacgcgcg aacgtcttta aaatgaacgg caaatggtac ctgttcactg actcccgcgg
     9481 atcaaaaatg acgattgacg gcattacgtc taacgatatt tacatgcttg gttatgtttc
     9541 taattcttta actggcccat acaagccgct gaacaaaact ggccttgtgt taaaaatgga
     9601 tcttgatcct aacgatgtaa cctttactta ctcacacttc gctgtacctc aagcgaaagg
     9661 aaacaatgtc gtgattacaa gctatatgac aaacagagga ttctacgcag acaaacaatc
     9721 aacgtttgcg ccaagcttcc tgctgaacat caaaggcaag aaaacatctg ttgtcaaaga
     9781 cagcatcctt gaacaaggac aattaacagt taacaaataa atgtcaggct cccttataca
     9841 cagccagtct gcacgtcgca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
     9901 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
     9961 tcaacttttt aatacaaagt tgcctccgga acataatggt gcatctagat gcccattcct
    10021 gtagacttct taattaagac gtcagaattc tcgaggcggc cgcatgtgag tctccctata
    10081 gtgagtcgta ttaatttcgc gggcgcgtca gcgggagagc cgttgtaagg cggca
//
